Estimation of high-order aberrations and anisotropic magnification from cryo-EM data sets in RELION -3.1

Methods are presented that detect three types of aberrations in single-particle cryo-EM data sets: symmetrical and antisymmetrical optical aberrations and magnification anisotropy. Because these methods only depend on the availability of a preliminary 3D reconstruction from the data, they can be use...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:IUCrJ Jg. 7; H. 2; S. 253 - 267
Hauptverfasser: Zivanov, Jasenko, Nakane, Takanori, Scheres, Sjors H. W.
Format: Journal Article
Sprache:Englisch
Veröffentlicht: England International Union of Crystallography 01.03.2020
Schlagworte:
Aberration
aberrations
Algorithms
Analysis
anisotropic magnification
Anisotropy
cryo-em
Datasets
Detection equipment
Microscopy
Open source software
Optical equipment
Radiation (Physics)
Reconstruction
relion
Research Papers
Source code
Technology
anisotropic magnification
aberrations
RELION
cryo-EM
ISSN:2052-2525, 2052-2525
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Methods are presented that detect three types of aberrations in single-particle cryo-EM data sets: symmetrical and antisymmetrical optical aberrations and magnification anisotropy. Because these methods only depend on the availability of a preliminary 3D reconstruction from the data, they can be used to correct for these aberrations for any given cryo-EM data set, a posteriori . Using five publicly available data sets, it is shown that considering these aberrations improves the resolution of the 3D reconstruction when these effects are present. The methods are implemented in version 3.1 of the open-source software package RELION .
Bibliographie:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 14
content type line 23
ISSN:2052-2525
2052-2525
DOI:10.1107/S2052252520000081