Estimation of high-order aberrations and anisotropic magnification from cryo-EM data sets in RELION -3.1

Methods are presented that detect three types of aberrations in single-particle cryo-EM data sets: symmetrical and antisymmetrical optical aberrations and magnification anisotropy. Because these methods only depend on the availability of a preliminary 3D reconstruction from the data, they can be use...

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Bibliographic Details
Published in:	IUCrJ Vol. 7; no. 2; pp. 253 - 267
Main Authors:	Zivanov, Jasenko, Nakane, Takanori, Scheres, Sjors H. W.
Format:	Journal Article
Language:	English
Published:	England International Union of Crystallography 01.03.2020
Subjects:	Aberration aberrations Algorithms Analysis anisotropic magnification Anisotropy cryo-em Datasets Detection equipment Microscopy Open source software Optical equipment Radiation (Physics) Reconstruction relion Research Papers Source code Technology anisotropic magnification aberrations RELION cryo-EM
ISSN:	2052-2525, 2052-2525
Online Access:	Get full text
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