Preparation of a Trp-BODIPY fluorogenic amino acid to label peptides for enhanced live-cell fluorescence imaging

Fluorescent peptides with excellent target specificity are useful imaging probes. This protocol describes the synthesis of a tryptophan-based fluorogenic amino acid (Fmoc-Trp(C 2 -BODIPY)-OH) and its incorporation into peptides for live-cell imaging. Fluorescent peptides are valuable tools for live-...

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Bibliographic Details
Published in:	Nature protocols Vol. 12; no. 8; pp. 1588 - 1619
Main Authors:	Mendive-Tapia, Lorena, Subiros-Funosas, Ramon, Zhao, Can, Albericio, Fernando, Read, Nick D, Lavilla, Rodolfo, Vendrell, Marc
Format:	Journal Article
Language:	English
Published:	London Nature Publishing Group UK 01.08.2017 Nature Publishing Group
Subjects:	14/10 14/34 14/63 631/1647/328/1978 631/92/611 639/624/1107/510 639/638/403/933 Amino acids Analysis Analytical Chemistry Antiinfectives and antibacterials Antimicrobial peptides Aspergillus fumigatus - cytology Biological Techniques Boron Compounds - analysis Boron Compounds - chemical synthesis Chemical synthesis Complications and side effects Computational Biology/Bioinformatics Dosage and administration Fluorescence Fungi Health aspects Imaging Life Sciences Lipophilic Microarrays Microbiological Techniques - methods Microscopy Molecular interactions Optical Imaging - methods Organic Chemistry Pathogenic microorganisms Pathogens Peptides protocol Solid phase methods Solid phase synthesis Spacer Staining and Labeling - methods Substrates Tryptophan Tryptophan - analysis Tryptophan - chemical synthesis
ISSN:	1754-2189, 1750-2799, 1750-2799
Online Access:	Get full text
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