The oral microbiota of patients with recurrent aphthous stomatitis

Specific pathogenic bacteria have been implicated in recurrent aphthous stomatitis (RAS), a chronic inflammatory condition characterised by ulcerations in the oral mucosa. However, the aetiology behind this condition still remains unclear. The buccal microbiota of patients with RAS was compared to t...

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Vydané v:Journal of oral microbiology Ročník 6; číslo 1; s. 25739 - 11
Hlavní autori: Bankvall, Maria, Sjöberg, Fei, Gale, Gita, Wold, Agnes, Jontell, Mats, Östman, Sofia
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: United States Taylor & Francis 01.01.2014
Taylor & Francis Ltd
Co-Action Publishing
Taylor & Francis Group
Predmet:
Antigens
Bacteria
Clinics
Dental health
Disease
Full text
Lesions
Methods
Microbiology
Multiculturalism & pluralism
Odontologi
Odontology
oral bacteria
oral cavity
oral microbiota
oral mucosa
oral mucosal disease
oral ulceration
Original
Polymerase chain reaction
recurrent aphtous stomatitis
Studies
T-RFLP
Terminal-Restriction Fragment Length Polymorphism
Ulcers
Sweden
oral cavity
oral bacteria
oral mucosal disease
Terminal-Restriction Fragment Length Polymorphism
oral mucosa
oral ulceration
ISSN:2000-2297, 2000-2297
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Shrnutí:Specific pathogenic bacteria have been implicated in recurrent aphthous stomatitis (RAS), a chronic inflammatory condition characterised by ulcerations in the oral mucosa. However, the aetiology behind this condition still remains unclear. The buccal microbiota of patients with RAS was compared to that of control subjects to investigate its potential role for this condition. Buccal swabs were obtained from non-ulcerative areas of 60 patients, of whom 42 patients had lesions at the time of sampling, and 60 healthy age- and gender-matched controls. Bacterial DNA was extracted and analysed by Terminal-Restriction Fragment Length Polymorphism, using enzymatic digestion of the polymerase chain reaction-amplified 16S rRNA gene, yielding a series of peaks, each representing a bacterial taxon. Two peaks, 60 and 489, were more prevalent in patients with RAS than controls. Conversely, peaks 58 and 490 were less common in patients than controls. When the patients were divided into subgroups, we found that the observed differences in peak-pattern were related to the presence of lesions during sampling. The microbiota of the non-inflamed buccal mucosa differed between patients and controls. The differences were most pronounced in patients who presented with lesions during sampling, suggesting that a disturbance in the normal buccal microbiota triggers the presence of lesions or that presence of lesions alters the microbiota.
Bibliografia:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:2000-2297
2000-2297
DOI:10.3402/jom.v6.25739