Transcriptome Analysis of Human Peripheral Blood Mononuclear Cells Exposed to Lassa Virus and to the Attenuated Mopeia/Lassa Reassortant 29 (ML29), a Vaccine Candidate
Lassa virus (LASV) is the causative agent of Lassa Fever and is responsible for several hundred thousand infections and thousands of deaths annually in West Africa. LASV and the non-pathogenic Mopeia virus (MOPV) are both rodent-borne African arenaviruses. A live attenuated reassortant of MOPV and L...
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| Published in: | PLoS neglected tropical diseases Vol. 7; no. 9; p. e2406 |
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| Main Authors: | , , , , , , , , , , , |
| Format: | Journal Article |
| Language: | English |
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Public Library of Science
01.09.2013
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| ISSN: | 1935-2735, 1935-2727, 1935-2735 |
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| Abstract | Lassa virus (LASV) is the causative agent of Lassa Fever and is responsible for several hundred thousand infections and thousands of deaths annually in West Africa. LASV and the non-pathogenic Mopeia virus (MOPV) are both rodent-borne African arenaviruses. A live attenuated reassortant of MOPV and LASV, designated ML29, protects rodents and primates from LASV challenge and appears to be more attenuated than MOPV. To gain better insight into LASV-induced pathology and mechanism of attenuation we performed gene expression profiling in human peripheral blood mononuclear cells (PBMC) exposed to LASV and the vaccine candidate ML29. PBMC from healthy human subjects were exposed to either LASV or ML29. Although most PBMC are non-permissive for virus replication, they remain susceptible to signal transduction by virus particles. Total RNA was extracted and global gene expression was evaluated during the first 24 hours using high-density microarrays. Results were validated using RT-PCR, flow cytometry and ELISA. LASV and ML29 elicited differential expression of interferon-stimulated genes (ISG), as well as genes involved in apoptosis, NF-kB signaling and the coagulation pathways. These genes could eventually serve as biomarkers to predict disease outcomes. The remarkable differential expression of thrombomodulin, a key regulator of inflammation and coagulation, suggests its involvement with vascular abnormalities and mortality in Lassa fever disease. |
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| AbstractList | Lassa virus (LASV) is the causative agent of Lassa Fever and is responsible for several hundred thousand infections and thousands of deaths annually in West Africa. LASV and the non-pathogenic Mopeia virus (MOPV) are both rodent-borne African arenaviruses. A live attenuated reassortant of MOPV and LASV, designated ML29, protects rodents and primates from LASV challenge and appears to be more attenuated than MOPV. To gain better insight into LASV-induced pathology and mechanism of attenuation we performed gene expression profiling in human peripheral blood mononuclear cells (PBMC) exposed to LASV and the vaccine candidate ML29. PBMC from healthy human subjects were exposed to either LASV or ML29. Although most PBMC are non-permissive for virus replication, they remain susceptible to signal transduction by virus particles. Total RNA was extracted and global gene expression was evaluated during the first 24 hours using high-density microarrays. Results were validated using RT-PCR, flow cytometry and ELISA. LASV and ML29 elicited differential expression of interferon-stimulated genes (ISG), as well as genes involved in apoptosis, NF-kB signaling and the coagulation pathways. These genes could eventually serve as biomarkers to predict disease outcomes. The remarkable differential expression of thrombomodulin, a key regulator of inflammation and coagulation, suggests its involvement with vascular abnormalities and mortality in Lassa fever disease. Lassa virus (LASV) is the causative agent of Lassa Fever and is responsible for several hundred thousand infections and thousands of deaths annually in West Africa. LASV and the non-pathogenic Mopeia virus (MOPV) are both rodent-borne African arenaviruses. A live attenuated reassortant of MOPV and LASV, designated ML29, protects rodents and primates from LASV challenge and appears to be more attenuated than MOPV. To gain better insight into LASV-induced pathology and mechanism of attenuation we performed gene expression profiling in human peripheral blood mononuclear cells (PBMC) exposed to LASV and the vaccine candidate ML29. PBMC from healthy human subjects were exposed to either LASV or ML29. Although most PBMC are non-permissive for virus replication, they remain susceptible to signal transduction by virus particles. Total RNA was extracted and global gene expression was evaluated during the first 24 hours using high-density microarrays. Results were validated using RT-PCR, flow cytometry and ELISA. LASV and ML29 elicited differential expression of interferon-stimulated genes (ISG), as well as genes involved in apoptosis, NF-kB signaling and the coagulation pathways. These genes could eventually serve as biomarkers to predict disease outcomes. The remarkable differential expression of thrombomodulin, a key regulator of inflammation and coagulation, suggests its involvement with vascular abnormalities and mortality in Lassa fever disease. Lassa virus (LASV) is the causative agent of Lassa Fever and is responsible for several hundred thousand infections and thousands of deaths annually in West Africa. LASV and the non-pathogenic Mopeia virus (MOPV) are both rodent-borne African arenaviruses. A live attenuated reassortant of MOPV and LASV, designated ML29, protects rodents and primates from LASV challenge and appears to be more attenuated than MOPV. To gain better insight into LASV-induced pathology and mechanism of attenuation we performed gene expression profiling in human peripheral blood mononuclear cells (PBMC) exposed to LASV and the vaccine candidate ML29. PBMC from healthy human subjects were exposed to either LASV or ML29. Although most PBMC are non-permissive for virus replication, they remain susceptible to signal transduction by virus particles. Total RNA was extracted and global gene expression was evaluated during the first 24 hours using high-density microarrays. Results were validated using RT-PCR, flow cytometry and ELISA. LASV and ML29 elicited differential expression of interferon-stimulated genes (ISG), as well as genes involved in apoptosis, NF-kB signaling and the coagulation pathways. These genes could eventually serve as biomarkers to predict disease outcomes. The remarkable differential expression of thrombomodulin, a key regulator of inflammation and coagulation, suggests its involvement with vascular abnormalities and mortality in Lassa fever disease. The virulent Lassa fever virus (LASV) and the non-pathogenic Mopeia virus (MOPV) infect rodents and, incidentally, people in West Africa. The mechanism of LASV damage in human beings is unclear. There is no licensed Lassa fever vaccine and therapeutic intervention is usually too late. The ML29 vaccine candidate derived from Lassa and Mopeia viruses protects rodents and primates from Lassa fever disease. Peripheral blood mononuclear cells from healthy human subjects were exposed to either LASV or ML29 in order to identify early cellular responses that could be attributed to the difference in virulence between the two viruses. Differential expression of interferon-stimulated genes as well as coagulation-related genes could lead to an explanation for Lassa fever pathogenesis and indicate protective treatments for Lassa fever disease. Lassa virus (LASV) is the causative agent of Lassa Fever and is responsible for several hundred thousand infections and thousands of deaths annually in West Africa. LASV and the non-pathogenic Mopeia virus (MOPV) are both rodent-borne African arenaviruses. A live attenuated reassortant of MOPV and LASV, designated ML29, protects rodents and primates from LASV challenge and appears to be more attenuated than MOPV. To gain better insight into LASV-induced pathology and mechanism of attenuation we performed gene expression profiling in human peripheral blood mononuclear cells (PBMC) exposed to LASV and the vaccine candidate ML29. PBMC from healthy human subjects were exposed to either LASV or ML29. Although most PBMC are non-permissive for virus replication, they remain susceptible to signal transduction by virus particles. Total RNA was extracted and global gene expression was evaluated during the first 24 hours using high-density microarrays. Results were validated using RT-PCR, flow cytometry and ELISA. LASV and ML29 elicited differential expression of interferon-stimulated genes (ISG), as well as genes involved in apoptosis, NF-kB signaling and the coagulation pathways. These genes could eventually serve as biomarkers to predict disease outcomes. The remarkable differential expression of thrombomodulin, a key regulator of inflammation and coagulation, suggests its involvement with vascular abnormalities and mortality in Lassa fever disease.Lassa virus (LASV) is the causative agent of Lassa Fever and is responsible for several hundred thousand infections and thousands of deaths annually in West Africa. LASV and the non-pathogenic Mopeia virus (MOPV) are both rodent-borne African arenaviruses. A live attenuated reassortant of MOPV and LASV, designated ML29, protects rodents and primates from LASV challenge and appears to be more attenuated than MOPV. To gain better insight into LASV-induced pathology and mechanism of attenuation we performed gene expression profiling in human peripheral blood mononuclear cells (PBMC) exposed to LASV and the vaccine candidate ML29. PBMC from healthy human subjects were exposed to either LASV or ML29. Although most PBMC are non-permissive for virus replication, they remain susceptible to signal transduction by virus particles. Total RNA was extracted and global gene expression was evaluated during the first 24 hours using high-density microarrays. Results were validated using RT-PCR, flow cytometry and ELISA. LASV and ML29 elicited differential expression of interferon-stimulated genes (ISG), as well as genes involved in apoptosis, NF-kB signaling and the coagulation pathways. These genes could eventually serve as biomarkers to predict disease outcomes. The remarkable differential expression of thrombomodulin, a key regulator of inflammation and coagulation, suggests its involvement with vascular abnormalities and mortality in Lassa fever disease. |
| Audience | Academic |
| Author | Patterson, Jean L. Mani, Sachin Poonia, Bhawna Crasta, Oswald Djavani, Mahmoud White, David M. Lukashevich, Igor S. Zhang, Yan Jett, Marti Zapata, Juan Carlos Carrion, Ricardo Salvato, Maria S. |
| AuthorAffiliation | 1 Institute of Human Virology, University of Maryland School of Medicine, Baltimore, Maryland, United States of America 4 Walter Reed Army Institute of Research, Silver Spring, Maryland, United States of America 2 Texas Biomedical Research Institute, San Antonio, Texas, United States of America 3 Virginia Bioinformatics Institute at Virginia Tech, Blacksburg, Virginia, United States of America 5 Center for Disease Control and Prevention, Atlanta, Georgia, United States of America University of Texas Medical Branch, United States of America |
| AuthorAffiliation_xml | – name: 3 Virginia Bioinformatics Institute at Virginia Tech, Blacksburg, Virginia, United States of America – name: 5 Center for Disease Control and Prevention, Atlanta, Georgia, United States of America – name: University of Texas Medical Branch, United States of America – name: 4 Walter Reed Army Institute of Research, Silver Spring, Maryland, United States of America – name: 2 Texas Biomedical Research Institute, San Antonio, Texas, United States of America – name: 1 Institute of Human Virology, University of Maryland School of Medicine, Baltimore, Maryland, United States of America |
| Author_xml | – sequence: 1 givenname: Juan Carlos surname: Zapata fullname: Zapata, Juan Carlos – sequence: 2 givenname: Ricardo surname: Carrion fullname: Carrion, Ricardo – sequence: 3 givenname: Jean L. surname: Patterson fullname: Patterson, Jean L. – sequence: 4 givenname: Oswald surname: Crasta fullname: Crasta, Oswald – sequence: 5 givenname: Yan surname: Zhang fullname: Zhang, Yan – sequence: 6 givenname: Sachin surname: Mani fullname: Mani, Sachin – sequence: 7 givenname: Marti surname: Jett fullname: Jett, Marti – sequence: 8 givenname: Bhawna surname: Poonia fullname: Poonia, Bhawna – sequence: 9 givenname: Mahmoud surname: Djavani fullname: Djavani, Mahmoud – sequence: 10 givenname: David M. surname: White fullname: White, David M. – sequence: 11 givenname: Igor S. surname: Lukashevich fullname: Lukashevich, Igor S. – sequence: 12 givenname: Maria S. surname: Salvato fullname: Salvato, Maria S. |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/24069471$$D View this record in MEDLINE/PubMed |
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| Cites_doi | 10.1096/fj.05-5619fje 10.1111/j.1365-2141.1989.tb04321.x 10.1128/JVI.74.15.6777-6783.2000 10.1038/nature02343 10.4049/jimmunol.163.10.5427 10.1111/j.1600-6143.2009.02652.x 10.1073/pnas.94.25.13985 10.1097/00007890-197105000-00010 10.1371/journal.pntd.0002171 10.1007/s11262-006-0050-3 10.1016/j.micinf.2006.02.002 10.1586/eri.10.142 10.1093/infdis/155.3.465 10.1172/JCI12980 10.1586/14760584.3.2.189 10.1172/JCI7811 10.1016/j.mib.2008.06.001 10.2202/1544-6115.1027 10.1038/nm822 10.1083/jcb.106.4.1359 10.1182/blood-2010-05-286872 10.3201/eid1205.050052 10.1099/vir.0.044099-0 10.1128/JVI.01948-08 10.1016/j.vaccine.2008.07.057 10.1128/JVI.78.17.9499-9511.2004 10.2174/138920206778948673 10.1111/j.1432-1033.1994.00877.x 10.1016/j.cell.2009.12.017 10.4049/jimmunol.171.11.6014 10.1016/j.vaccine.2007.02.038 10.1371/journal.ppat.1002337 10.1126/science.287.5454.860 10.1160/TH09-06-0357 10.1002/jmv.2206 10.1186/1743-422X-10-52 10.1038/nm1236 10.1093/bioinformatics/17.9.763 10.1198/016214504000000683 10.1099/vir.0.82529-0 10.1002/eji.1830251241 10.1161/01.RES.84.1.84 10.1096/fj.05-5328fje 10.1016/j.cytogfr.2003.10.002 10.4049/jimmunol.170.6.2797 10.1002/jlb.59.1.67 10.1111/j.1462-5822.2012.01784.x 10.1099/vir.0.2008/002048-0 10.4269/ajtmh.1970.19.670 10.1128/JVI.77.12.7101-7105.2003 10.1093/bioinformatics/bth456 10.1002/(SICI)1096-9071(199912)59:4<552::AID-JMV21>3.0.CO;2-A 10.1128/MCB.11.4.2125 10.1128/JVI.01384-06 10.4049/jimmunol.1001328 10.1093/nar/gng015 10.1016/B978-0-12-384684-6.00058-6 10.1371/journal.pone.0007892 10.1007/s00262-004-0645-2 10.1002/jmv.1890260204 10.1161/01.ATV.0000134298.25489.92 10.1073/pnas.95.25.14863 10.1681/ASN.V981464 10.1126/science.1168676 10.1083/jcb.108.5.1917 10.1074/jbc.M205900200 10.1186/1471-2334-8-104 10.1371/journal.pntd.0000710 10.4049/jimmunol.172.5.2861 10.1128/JVI.77.3.1727-1737.2003 10.1089/107999000414853 10.1172/JCI26989 10.1038/nm1142 10.1086/320722 10.2174/157340611794859343 10.1186/1743-422X-4-82 10.1371/journal.ppat.1001258 10.1371/journal.ppat.1000455 10.1093/infdis/155.3.437 10.1128/JVI.02570-08 10.1089/107999003772084833 10.1097/MBC.0b013e32801481cb 10.1172/JCI2869 10.1016/0042-6822(92)90514-P 10.1128/JVI.00536-07 10.1038/ni.1688 10.1016/j.bios.2004.06.043 10.1128/JVI.00576-12 10.1186/1743-422X-8-205 10.1016/S0021-9258(18)83649-3 10.1006/bbrc.1993.1251 10.1046/j.1469-7580.2000.19720207.x 10.1074/jbc.273.8.4289 10.1128/JVI.79.22.13934-13942.2005 |
| ContentType | Journal Article |
| Copyright | COPYRIGHT 2013 Public Library of Science 2013 2013 Public Library of Science. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited: Citation: Zapata JC, Carrion R Jr, Patterson JL, Crasta O, Zhang Y, et al. (2013) Transcriptome Analysis of Human Peripheral Blood Mononuclear Cells Exposed to Lassa Virus and to the Attenuated Mopeia/Lassa Reassortant 29 (ML29), a Vaccine Candidate. PLoS Negl Trop Dis 7(9): e2406. doi:10.1371/journal.pntd.0002406 |
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| Issue | 9 |
| Keywords | Flow Cytometry Enzyme-Linked Immunosorbent Assay Microarray Analysis Humans Viral Vaccines Reassortant Viruses Gene Expression Profiling Leukocytes, Mononuclear Lassa virus Reverse Transcriptase Polymerase Chain Reaction |
| Language | English |
| License | This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. Creative Commons Attribution License |
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| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Current address: Center for Predictive Medicine, University of Louisville, Louisville, Kentucky, United States of America. ISL and MSS are co-senior authors. Conceived and designed the experiments: JCZ ISL MSS. Performed the experiments: JCZ SM BP RC DMW. Analyzed the data: JCZ OC YZ MD ISL MSS. Contributed reagents/materials/analysis tools: MJ JLP OC YZ. Wrote the paper: JCZ ISL MSS. The authors have declared that no competing interests exist. |
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| References | H Zhu (ref31) 1997; 94 K Hagiwara (ref97) 1991; 11 EP Scott (ref85) 2008; 89 EJ Nascimento (ref71) 2009; 4 L Cai (ref52) 2011; 7 E Toniato (ref78) 2002; 277 HK Kim (ref45) 2007; 18 JM Rojek (ref67) 2012; 14 P Loke (ref72) 2010; 4 E Tahara Jr (ref46) 2005; 54 S Zhou (ref51) 2012; 86 R Das (ref34) 2008; 8 DI Simon (ref96) 2000; 105 H Ulger (ref98) 2000; 197 Z Wu (ref42) 2004; 99 JD Frame (ref4) 1970; 19 GK Smyth (ref43) 2004; 3 D Chansel (ref58) 2006; 20 DT Denhardt (ref74) 2001; 107 S Malhotra (ref68) 2013; 7 G Opdenakker (ref81) 1993; 191 R Hammamieh (ref32) 2004; 20 JC Zapata (ref30) 2013; 10 X Dong (ref38) 1999; 163 C Myskiw (ref55) 2009; 83 S Baize (ref19) 2004; 172 TW Geisbert (ref12) 2004; 10 ref8 T Briese (ref9) 2009; 5 EM Feeley (ref48) 2011; 7 SP Fisher-Hoch (ref95) 1987; 134 S Kunz (ref86) 2009; 102 P Proost (ref82) 1996; 59 MM Djavani (ref24) 2007; 81 M Stremlau (ref79) 2004; 427 TD Querec (ref70) 2009; 10 JB McCormick (ref2) 2002; 262 JB McCormick (ref14) 1986; 314 X Wang (ref73) 2005; 11 JM Waugh (ref91) 1999; 84 J Rumbaugh (ref53) 2006; 20 ML Moraz (ref87) 2011; 9 JS Bennett (ref66) 2005; 115 D Cummins (ref88) 1989; 72 SP Fisher-Hoch (ref15) 1987; 155 WD Staatz (ref65) 1989; 108 GC Bowick (ref35) 2006; 80 LE Hensley (ref99) 2011; 8 S Mahanty (ref22) 2003; 170 A Navarro (ref94) 2003; 23 L Feng (ref59) 2000; 105 S Baize (ref20) 2009; 83 CT Esmon (ref90) 1989; 264 JC Kash (ref69) 2004; 78 S Fisher-Hoch (ref16) 1988; 26 IS Lukashevich (ref26) 2005; 79 IS Lukashevich (ref29) 2008; 26 IS Lukashevich (ref18) 1999; 59 JB McCormick (ref1) 1988; 62 Suppl G Kirkland (ref60) 1998; 9 RS Piotrowicz (ref64) 1988; 106 SP Fisher-Hoch (ref89) 2000; 74 R Carrion Jr (ref28) 2007; 25 RA Irizarry (ref41) 2003; 31 JB McCormick (ref5) 1987; 155 S Muller (ref36) 2007; 88 E Izmailova (ref77) 2003; 9 AV Chee (ref80) 2003; 77 RN Charrel (ref11) 2008; 11 VB Mehta (ref61) 2003; 171 MB Eisen (ref39) 1998; 95 EI Boyle (ref44) 2004; 20 S Baize (ref50) 2006; 8 S Ashkar (ref54) 2000; 287 A Ishii (ref10) 2012; 93 D Nanba (ref62) 2004; 15 R Hammamieh (ref33) 2007; 4 IC Huang (ref49) 2011; 7 IS Lukashevich (ref23) 2002; 67 A Kitamura (ref76) 1994; 224 IS Lukashevich (ref17) 2003; 77 IS Lukashevich (ref25) 1992; 188 T Standal (ref75) 2004; 26 IS Lukashevich (ref7) 2006; 7 M Van de Wouwer (ref92) 2004; 24 AN Stratman (ref63) 2010; 116 W Gong (ref83) 1998; 273 W Cui (ref93) 2009; 9 Q Li (ref13) 2009; 323 AM Macher (ref6) 2006; 12 KY Yeung (ref40) 2001; 17 SJ Roth (ref84) 1995; 25 SP Fisher-Hoch (ref3) 2004; 3 A D'Haese (ref56) 2000; 20 D Tripodi (ref37) 1971; 11 DA Moshkoff (ref27) 2007; 34 AL Brass (ref47) 2009; 139 S Mahanty (ref21) 2001; 183 PY Lee (ref57) 2011; 186 |
| References_xml | – volume: 20 start-page: 1736 year: 2006 ident: ref53 article-title: Interaction of HIV Tat and matrix metalloproteinase in HIV neuropathogenesis: a new host defense mechanism publication-title: FASEB journal: official publication of the Federation of American Societies for Experimental Biology doi: 10.1096/fj.05-5619fje – volume: 72 start-page: 543 year: 1989 ident: ref88 article-title: A plasma inhibitor of platelet aggregation in patients with Lassa fever publication-title: Br J Haematol doi: 10.1111/j.1365-2141.1989.tb04321.x – volume: 74 start-page: 6777 year: 2000 ident: ref89 article-title: Effective vaccine for lassa fever publication-title: Journal of virology doi: 10.1128/JVI.74.15.6777-6783.2000 – volume: 427 start-page: 848 year: 2004 ident: ref79 article-title: The cytoplasmic body component TRIM5alpha restricts HIV-1 infection in Old World monkeys publication-title: Nature doi: 10.1038/nature02343 – volume: 163 start-page: 5427 year: 1999 ident: ref38 article-title: Binding and uptake of agalactosyl IgG by mannose receptor on macrophages and dendritic cells publication-title: Journal of immunology doi: 10.4049/jimmunol.163.10.5427 – volume: 9 start-page: 1308 year: 2009 ident: ref93 article-title: Thrombomodulin improves early outcomes after intraportal islet transplantation publication-title: American journal of transplantation: official journal of the American Society of Transplantation and the American Society of Transplant Surgeons doi: 10.1111/j.1600-6143.2009.02652.x – volume: 94 start-page: 13985 year: 1997 ident: ref31 article-title: Use of differential display analysis to assess the effect of human cytomegalovirus infection on the accumulation of cellular RNAs: induction of interferon-responsive RNAs publication-title: Proceedings of the National Academy of Sciences of the United States of America doi: 10.1073/pnas.94.25.13985 – volume: 11 start-page: 487 year: 1971 ident: ref37 article-title: Separation of peripheral leukocytes by Ficoll density gradient centrifugation publication-title: Transplantation doi: 10.1097/00007890-197105000-00010 – volume: 7 start-page: e2171 year: 2013 ident: ref68 article-title: Transcriptional profiling of the circulating immune response to lassa virus in an aerosol model of exposure publication-title: PLoS neglected tropical diseases doi: 10.1371/journal.pntd.0002171 – volume: 34 start-page: 169 year: 2007 ident: ref27 article-title: Molecular characterization of a reassortant virus derived from Lassa and Mopeia viruses publication-title: Virus Genes doi: 10.1007/s11262-006-0050-3 – volume: 8 start-page: 1194 year: 2006 ident: ref50 article-title: Role of interferons in the control of Lassa virus replication in human dendritic cells and macrophages publication-title: Microbes and infection/Institut Pasteur doi: 10.1016/j.micinf.2006.02.002 – volume: 9 start-page: 49 year: 2011 ident: ref87 article-title: Pathogenesis of arenavirus hemorrhagic fevers publication-title: Expert review of anti-infective therapy doi: 10.1586/eri.10.142 – volume: 155 start-page: 465 year: 1987 ident: ref15 article-title: Physiological and immunologic disturbances associated with shock in a primate model of Lassa fever publication-title: J Infect Dis doi: 10.1093/infdis/155.3.465 – volume: 107 start-page: 1055 year: 2001 ident: ref74 article-title: Osteopontin as a means to cope with environmental insults: regulation of inflammation, tissue remodeling, and cell survival publication-title: The Journal of clinical investigation doi: 10.1172/JCI12980 – volume: 3 start-page: 189 year: 2004 ident: ref3 article-title: Lassa fever vaccine publication-title: Expert review of vaccines doi: 10.1586/14760584.3.2.189 – volume: 105 start-page: 293 year: 2000 ident: ref96 article-title: Decreased neointimal formation in Mac-1(−/−) mice reveals a role for inflammation in vascular repair after angioplasty publication-title: The Journal of clinical investigation doi: 10.1172/JCI7811 – volume: 11 start-page: 362 year: 2008 ident: ref11 article-title: Phylogeny of the genus Arenavirus publication-title: Current opinion in microbiology doi: 10.1016/j.mib.2008.06.001 – volume: 3 start-page: Article 3 year: 2004 ident: ref43 article-title: Linear models and empirical bayes methods for assessing differential expression in microarray experiments publication-title: Statistical applications in genetics and molecular biology doi: 10.2202/1544-6115.1027 – volume: 9 start-page: 191 year: 2003 ident: ref77 article-title: HIV-1 Tat reprograms immature dendritic cells to express chemoattractants for activated T cells and macrophages publication-title: Nature Medicine doi: 10.1038/nm822 – volume: 106 start-page: 1359 year: 1988 ident: ref64 article-title: Glycoprotein Ic-IIa functions as an activation-independent fibronectin receptor on human platelets publication-title: The Journal of cell biology doi: 10.1083/jcb.106.4.1359 – volume: 314 start-page: 20 year: 1986 ident: ref14 article-title: Lassa fever. Effective therapy with ribavirin publication-title: N Engl J Med – volume: 116 start-page: 4720 year: 2010 ident: ref63 article-title: Endothelial-derived PDGF-BB and HB-EGF coordinately regulate pericyte recruitment during vasculogenic tube assembly and stabilization publication-title: Blood doi: 10.1182/blood-2010-05-286872 – volume: 12 start-page: 835 year: 2006 ident: ref6 article-title: Historical Lassa fever reports and 30-year clinical update publication-title: Emerging infectious diseases doi: 10.3201/eid1205.050052 – volume: 93 start-page: 2247 year: 2012 ident: ref10 article-title: Molecular surveillance and phylogenetic analysis of Old World arenaviruses in Zambia publication-title: The Journal of general virology doi: 10.1099/vir.0.044099-0 – volume: 83 start-page: 5890 year: 2009 ident: ref20 article-title: Early and strong immune responses are associated with control of viral replication and recovery in lassa virus-infected cynomolgus monkeys publication-title: J Virol doi: 10.1128/JVI.01948-08 – volume: 26 start-page: 5246 year: 2008 ident: ref29 article-title: Safety, immunogenicity, and efficacy of the ML29 reassortant vaccine for Lassa fever in small non-human primates publication-title: Vaccine doi: 10.1016/j.vaccine.2008.07.057 – volume: 78 start-page: 9499 year: 2004 ident: ref69 article-title: Global host immune response: pathogenesis and transcriptional profiling of type A influenza viruses expressing the hemagglutinin and neuraminidase genes from the 1918 pandemic virus publication-title: Journal of virology doi: 10.1128/JVI.78.17.9499-9511.2004 – volume: 7 start-page: 351 year: 2006 ident: ref7 article-title: Lassa Virus Genome publication-title: Current Genomics doi: 10.2174/138920206778948673 – volume: 224 start-page: 877 year: 1994 ident: ref76 article-title: Induction of the human gene for p44, a hepatitis-C-associated microtubular aggregate protein, by interferon-alpha/beta publication-title: European journal of biochemistry/FEBS doi: 10.1111/j.1432-1033.1994.00877.x – volume: 139 start-page: 1243 year: 2009 ident: ref47 article-title: The IFITM proteins mediate cellular resistance to influenza A H1N1 virus, West Nile virus, and dengue virus publication-title: Cell doi: 10.1016/j.cell.2009.12.017 – volume: 171 start-page: 6014 year: 2003 ident: ref61 article-title: Inhibition of NF-kappa B activation and its target genes by heparin-binding epidermal growth factor-like growth factor publication-title: Journal of immunology doi: 10.4049/jimmunol.171.11.6014 – volume: 25 start-page: 4093 year: 2007 ident: ref28 article-title: A ML29 reassortant virus protects guinea pigs against a distantly related Nigerian strain of Lassa virus and can provide sterilizing immunity publication-title: Vaccine doi: 10.1016/j.vaccine.2007.02.038 – volume: 262 start-page: 75 year: 2002 ident: ref2 article-title: Lassa fever publication-title: Curr Top Microbiol Immunol – volume: 7 start-page: e1002337 year: 2011 ident: ref48 article-title: IFITM3 inhibits influenza A virus infection by preventing cytosolic entry publication-title: PLoS pathogens doi: 10.1371/journal.ppat.1002337 – volume: 287 start-page: 860 year: 2000 ident: ref54 article-title: Eta-1 (osteopontin): an early component of type-1 (cell-mediated) immunity publication-title: Science doi: 10.1126/science.287.5454.860 – volume: 102 start-page: 1024 year: 2009 ident: ref86 article-title: The role of the vascular endothelium in arenavirus haemorrhagic fevers publication-title: Thrombosis and haemostasis doi: 10.1160/TH09-06-0357 – volume: 67 start-page: 171 year: 2002 ident: ref23 article-title: Hemorrhagic fever occurs after intravenous, but not after intragastric, inoculation of rhesus macaques with lymphocytic choriomeningitis virus publication-title: J Med Virol doi: 10.1002/jmv.2206 – volume: 10 start-page: 52 year: 2013 ident: ref30 article-title: An attenuated Lassa vaccine in SIV-infected rhesus macaques does not persist or cause arenavirus disease but does elicit Lassa virus-specific immunity publication-title: Virology journal doi: 10.1186/1743-422X-10-52 – volume: 11 start-page: 515 year: 2005 ident: ref73 article-title: Integrin alphavbeta3 is a coreceptor for human cytomegalovirus publication-title: Nature Medicine doi: 10.1038/nm1236 – volume: 17 start-page: 763 year: 2001 ident: ref40 article-title: Principal component analysis for clustering gene expression data publication-title: Bioinformatics doi: 10.1093/bioinformatics/17.9.763 – volume: 99 start-page: 909 year: 2004 ident: ref42 article-title: A model based background adjustment for oligonucleotide expression arrays publication-title: J Am Stat Assoc doi: 10.1198/016214504000000683 – volume: 88 start-page: 1568 year: 2007 ident: ref36 article-title: Analysis of gene expression in Lassa virus-infected HuH-7 cells publication-title: The Journal of general virology doi: 10.1099/vir.0.82529-0 – volume: 25 start-page: 3482 year: 1995 ident: ref84 article-title: C-C chemokines, but not the C-X-C chemokines interleukin-8 and interferon-gamma inducible protein-10, stimulate transendothelial chemotaxis of T lymphocytes publication-title: European journal of immunology doi: 10.1002/eji.1830251241 – volume: 84 start-page: 84 year: 1999 ident: ref91 article-title: Local overexpression of thrombomodulin for in vivo prevention of arterial thrombosis in a rabbit model publication-title: Circulation research doi: 10.1161/01.RES.84.1.84 – volume: 20 start-page: 1936 year: 2006 ident: ref58 article-title: Heparin binding EGF is necessary for vasospastic response to endothelin publication-title: FASEB journal: official publication of the Federation of American Societies for Experimental Biology doi: 10.1096/fj.05-5328fje – volume: 15 start-page: 13 year: 2004 ident: ref62 article-title: Dual intracellular signaling by proteolytic cleavage of membrane-anchored heparin-binding EGF-like growth factor publication-title: Cytokine & growth factor reviews doi: 10.1016/j.cytogfr.2003.10.002 – volume: 62 Suppl start-page: 353 year: 1988 ident: ref1 article-title: Lassa fever: epidemiology, therapy and vaccine development publication-title: Kansenshogaku Zasshi – volume: 170 start-page: 2797 year: 2003 ident: ref22 article-title: Cutting edge: impairment of dendritic cells and adaptive immunity by Ebola and Lassa viruses publication-title: J Immunol doi: 10.4049/jimmunol.170.6.2797 – volume: 59 start-page: 67 year: 1996 ident: ref82 article-title: Human monocyte chemotactic proteins-2 and -3: structural and functional comparison with MCP-1 publication-title: Journal of leukocyte biology doi: 10.1002/jlb.59.1.67 – volume: 14 start-page: 1122 year: 2012 ident: ref67 article-title: Binding of Lassa virus perturbs extracellular matrix-induced signal transduction via dystroglycan publication-title: Cellular microbiology doi: 10.1111/j.1462-5822.2012.01784.x – volume: 89 start-page: 2569 year: 2008 ident: ref85 article-title: Cytokine patterns in a comparative model of arenavirus haemorrhagic fever in guinea pigs publication-title: The Journal of general virology doi: 10.1099/vir.0.2008/002048-0 – volume: 19 start-page: 670 year: 1970 ident: ref4 article-title: Lassa fever, a new virus disease of man from West Africa. I. Clinical description and pathological findings publication-title: The American journal of tropical medicine and hygiene doi: 10.4269/ajtmh.1970.19.670 – volume: 77 start-page: 7101 year: 2003 ident: ref80 article-title: Promyelocytic leukemia protein mediates interferon-based anti-herpes simplex virus 1 effects publication-title: Journal of virology doi: 10.1128/JVI.77.12.7101-7105.2003 – volume: 20 start-page: 3710 year: 2004 ident: ref44 article-title: GO::TermFinder–open source software for accessing Gene Ontology information and finding significantly enriched Gene Ontology terms associated with a list of genes publication-title: Bioinformatics doi: 10.1093/bioinformatics/bth456 – volume: 59 start-page: 552 year: 1999 ident: ref18 article-title: Lassa and Mopeia virus replication in human monocytes/macrophages and in endothelial cells: different effects on IL-8 and TNF-alpha gene expression publication-title: J Med Virol doi: 10.1002/(SICI)1096-9071(199912)59:4<552::AID-JMV21>3.0.CO;2-A – volume: 11 start-page: 2125 year: 1991 ident: ref97 article-title: Organization and chromosomal localization of the human platelet-derived endothelial cell growth factor gene publication-title: Molecular and cellular biology doi: 10.1128/MCB.11.4.2125 – volume: 80 start-page: 10248 year: 2006 ident: ref35 article-title: Differential signaling networks induced by mild and lethal hemorrhagic fever virus infections publication-title: Journal of virology doi: 10.1128/JVI.01384-06 – volume: 186 start-page: 1747 year: 2011 ident: ref57 article-title: IL-1alpha modulates neutrophil recruitment in chronic inflammation induced by hydrocarbon oil publication-title: Journal of immunology doi: 10.4049/jimmunol.1001328 – volume: 31 start-page: e15 year: 2003 ident: ref41 article-title: Summaries of Affymetrix GeneChip probe level data publication-title: Nucleic acids research doi: 10.1093/nar/gng015 – ident: ref8 doi: 10.1016/B978-0-12-384684-6.00058-6 – volume: 4 start-page: e7892 year: 2009 ident: ref71 article-title: Gene expression profiling during early acute febrile stage of dengue infection can predict the disease outcome publication-title: PLoS One doi: 10.1371/journal.pone.0007892 – volume: 54 start-page: 729 year: 2005 ident: ref46 article-title: G1P3, an interferon inducible gene 6–16, is expressed in gastric cancers and inhibits mitochondrial-mediated apoptosis in gastric cancer cell line TMK-1 cell publication-title: Cancer immunology, immunotherapy: CII doi: 10.1007/s00262-004-0645-2 – volume: 26 start-page: 127 year: 1988 ident: ref16 article-title: Hematologic dysfunction in Lassa fever publication-title: J Med Virol doi: 10.1002/jmv.1890260204 – volume: 24 start-page: 1374 year: 2004 ident: ref92 article-title: Thrombomodulin-protein C-EPCR system: integrated to regulate coagulation and inflammation publication-title: Arteriosclerosis, thrombosis, and vascular biology doi: 10.1161/01.ATV.0000134298.25489.92 – volume: 95 start-page: 14863 year: 1998 ident: ref39 article-title: Cluster analysis and display of genome-wide expression patterns publication-title: Proceedings of the National Academy of Sciences of the United States of America doi: 10.1073/pnas.95.25.14863 – volume: 9 start-page: 1464 year: 1998 ident: ref60 article-title: Heparin-binding EGF-like growth factor mRNA is upregulated in the peri-infarct region of the remnant kidney model: in vitro evidence suggests a regulatory role in myofibroblast transformation publication-title: Journal of the American Society of Nephrology: JASN doi: 10.1681/ASN.V981464 – volume: 323 start-page: 1726 year: 2009 ident: ref13 article-title: Visualizing antigen-specific and infected cells in situ predicts outcomes in early viral infection publication-title: Science doi: 10.1126/science.1168676 – volume: 108 start-page: 1917 year: 1989 ident: ref65 article-title: The membrane glycoprotein Ia–IIa (VLA-2) complex mediates the Mg++-dependent adhesion of platelets to collagen publication-title: The Journal of cell biology doi: 10.1083/jcb.108.5.1917 – volume: 277 start-page: 37315 year: 2002 ident: ref78 article-title: TRIM8/GERP RING finger protein interacts with SOCS-1 publication-title: The Journal of biological chemistry doi: 10.1074/jbc.M205900200 – volume: 8 start-page: 104 year: 2008 ident: ref34 article-title: Early indicators of exposure to biological threat agents using host gene profiles in peripheral blood mononuclear cells publication-title: BMC infectious diseases doi: 10.1186/1471-2334-8-104 – volume: 4 start-page: e710 year: 2010 ident: ref72 article-title: Gene expression patterns of dengue virus-infected children from nicaragua reveal a distinct signature of increased metabolism publication-title: PLoS neglected tropical diseases doi: 10.1371/journal.pntd.0000710 – volume: 172 start-page: 2861 year: 2004 ident: ref19 article-title: Lassa virus infection of human dendritic cells and macrophages is productive but fails to activate cells publication-title: J Immunol doi: 10.4049/jimmunol.172.5.2861 – volume: 77 start-page: 1727 year: 2003 ident: ref17 article-title: Arenavirus-mediated liver pathology: acute lymphocytic choriomeningitis virus infection of rhesus macaques is characterized by high-level interleukin-6 expression and hepatocyte proliferation publication-title: J Virol doi: 10.1128/JVI.77.3.1727-1737.2003 – volume: 20 start-page: 667 year: 2000 ident: ref56 article-title: In vivo neutrophil recruitment by granulocyte chemotactic protein-2 is assisted by gelatinase B/MMP-9 in the mouse publication-title: Journal of interferon & cytokine research: the official journal of the International Society for Interferon and Cytokine Research doi: 10.1089/107999000414853 – volume: 115 start-page: 3363 year: 2005 ident: ref66 article-title: Structure and function of the platelet integrin alphaIIbbeta3 publication-title: The Journal of clinical investigation doi: 10.1172/JCI26989 – volume: 10 start-page: S110 year: 2004 ident: ref12 article-title: Exotic emerging viral diseases: progress and challenges publication-title: Nature Medicine doi: 10.1038/nm1142 – volume: 183 start-page: 1713 year: 2001 ident: ref21 article-title: Low levels of interleukin-8 and interferon-inducible protein-10 in serum are associated with fatal infections in acute Lassa fever publication-title: J Infect Dis doi: 10.1086/320722 – volume: 134 start-page: 231 year: 1987 ident: ref95 article-title: Pathophysiology and treatment of Lassa fever publication-title: Curr Top Microbiol Immunol – volume: 7 start-page: 121 year: 2011 ident: ref52 article-title: Identification of proteins interacting with human SP110 during the process of viral infections publication-title: Medicinal chemistry doi: 10.2174/157340611794859343 – volume: 4 start-page: 82 year: 2007 ident: ref33 article-title: Blood genomic profiles of exposures to Venezuelan equine encephalitis in Cynomolgus macaques (Macaca fascicularis) publication-title: Virology journal doi: 10.1186/1743-422X-4-82 – volume: 7 start-page: e1001258 year: 2011 ident: ref49 article-title: Distinct patterns of IFITM-mediated restriction of filoviruses, SARS coronavirus, and influenza A virus publication-title: PLoS pathogens doi: 10.1371/journal.ppat.1001258 – volume: 5 start-page: e1000455 year: 2009 ident: ref9 article-title: Genetic detection and characterization of Lujo virus, a new hemorrhagic fever-associated arenavirus from southern Africa publication-title: PLoS Pathog doi: 10.1371/journal.ppat.1000455 – volume: 155 start-page: 437 year: 1987 ident: ref5 article-title: A prospective study of the epidemiology and ecology of Lassa fever publication-title: J Infect Dis doi: 10.1093/infdis/155.3.437 – volume: 83 start-page: 6757 year: 2009 ident: ref55 article-title: Vaccinia virus E3 suppresses expression of diverse cytokines through inhibition of the PKR, NF-kappaB, and IRF3 pathways publication-title: Journal of virology doi: 10.1128/JVI.02570-08 – volume: 23 start-page: 723 year: 2003 ident: ref94 article-title: Thrombomodulin RNA is destabilized through its 3′-untranslated element in cells exposed to IFN-gamma publication-title: Journal of interferon & cytokine research: the official journal of the International Society for Interferon and Cytokine Research doi: 10.1089/107999003772084833 – volume: 18 start-page: 157 year: 2007 ident: ref45 article-title: Lipopolysaccharide down-regulates the thrombomodulin expression of peripheral blood monocytes: effect of serum on thrombomodulin expression in the THP-1 monocytic cell line publication-title: Blood coagulation & fibrinolysis: an international journal in haemostasis and thrombosis doi: 10.1097/MBC.0b013e32801481cb – volume: 105 start-page: 341 year: 2000 ident: ref59 article-title: Heparin-binding EGF-like growth factor contributes to reduced glomerular filtration rate during glomerulonephritis in rats publication-title: The Journal of clinical investigation doi: 10.1172/JCI2869 – volume: 188 start-page: 600 year: 1992 ident: ref25 article-title: Generation of reassortants between African arenaviruses publication-title: Virology doi: 10.1016/0042-6822(92)90514-P – volume: 81 start-page: 7960 year: 2007 ident: ref24 article-title: Early blood profiles of virus infection in a monkey model for Lassa fever publication-title: J Virol doi: 10.1128/JVI.00536-07 – volume: 10 start-page: 116 year: 2009 ident: ref70 article-title: Systems biology approach predicts immunogenicity of the yellow fever vaccine in humans publication-title: Nature immunology doi: 10.1038/ni.1688 – volume: 20 start-page: 719 year: 2004 ident: ref32 article-title: Modeling of SEB-induced host gene expression to correlate in vitro to in vivo responses publication-title: Biosensors & bioelectronics doi: 10.1016/j.bios.2004.06.043 – volume: 86 start-page: 11254 year: 2012 ident: ref51 article-title: Role of interferon regulatory factor 7 in T cell responses during acute lymphocytic choriomeningitis virus infection publication-title: Journal of virology doi: 10.1128/JVI.00576-12 – volume: 8 start-page: 205 year: 2011 ident: ref99 article-title: Pathogenesis of Lassa fever in cynomolgus macaques publication-title: Virology journal doi: 10.1186/1743-422X-8-205 – volume: 26 start-page: 179 year: 2004 ident: ref75 article-title: Role of osteopontin in adhesion, migration, cell survival and bone remodeling publication-title: Experimental oncology – volume: 264 start-page: 4743 year: 1989 ident: ref90 article-title: The roles of protein C and thrombomodulin in the regulation of blood coagulation publication-title: The Journal of biological chemistry doi: 10.1016/S0021-9258(18)83649-3 – volume: 191 start-page: 535 year: 1993 ident: ref81 article-title: Human monocyte chemotactic protein-3 (MCP-3): molecular cloning of the cDNA and comparison with other chemokines publication-title: Biochemical and biophysical research communications doi: 10.1006/bbrc.1993.1251 – volume: 197 start-page: 207 issue: Pt 2 year: 2000 ident: ref98 article-title: The growth promoting effects of bFGF, PD-ECGF and VEGF on cultured postimplantation rat embryos deprived of serum fractions publication-title: Journal of anatomy doi: 10.1046/j.1469-7580.2000.19720207.x – volume: 273 start-page: 4289 year: 1998 ident: ref83 article-title: Monocyte chemotactic protein-2 activates CCR5 and blocks CD4/CCR5-mediated HIV-1 entry/replication publication-title: The Journal of biological chemistry doi: 10.1074/jbc.273.8.4289 – volume: 79 start-page: 13934 year: 2005 ident: ref26 article-title: A live attenuated vaccine for Lassa fever made by reassortment of Lassa and Mopeia viruses publication-title: J Virol doi: 10.1128/JVI.79.22.13934-13942.2005 |
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| Snippet | Lassa virus (LASV) is the causative agent of Lassa Fever and is responsible for several hundred thousand infections and thousands of deaths annually in West... Lassa virus (LASV) is the causative agent of Lassa Fever and is responsible for several hundred thousand infections and thousands of deaths annually in West... |
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| SubjectTerms | Analysis Biology Blood Causes of Cluster analysis Disease Enzyme-Linked Immunosorbent Assay Fever Flow Cytometry Gene expression Gene Expression Profiling Genetic aspects Health aspects Health care Human subjects Humans Immune response Infections Interferon Lassa fever Lassa virus - growth & development Lassa virus - immunology Leukocytes, Mononuclear - immunology Leukocytes, Mononuclear - virology Medicine Microarray Analysis Mortality Pathogenesis Prevention Reassortant Viruses - growth & development Reassortant Viruses - immunology Reverse Transcriptase Polymerase Chain Reaction Rodents Signal transduction Vaccines Viral Vaccines - immunology Viruses |
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| Title | Transcriptome Analysis of Human Peripheral Blood Mononuclear Cells Exposed to Lassa Virus and to the Attenuated Mopeia/Lassa Reassortant 29 (ML29), a Vaccine Candidate |
| URI | https://www.ncbi.nlm.nih.gov/pubmed/24069471 https://www.proquest.com/docview/1437580802 https://pubmed.ncbi.nlm.nih.gov/PMC3772037 https://doaj.org/article/db9c4924deca43d4a4ecfeda84ade5ef http://dx.doi.org/10.1371/journal.pntd.0002406 |
| Volume | 7 |
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