Accelerated and Improved Differentiation of Retinal Organoids from Pluripotent Stem Cells in Rotating-Wall Vessel Bioreactors

Pluripotent stem cells can be differentiated into 3D retinal organoids, with major cell types self-patterning into a polarized, laminated architecture. In static cultures, organoid development may be hindered by limitations in diffusion of oxygen and nutrients. Herein, we report a bioprocess using r...

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Bibliographic Details
Published in:	Stem cell reports Vol. 10; no. 1; pp. 300 - 313
Main Authors:	DiStefano, Tyler, Chen, Holly Yu, Panebianco, Christopher, Kaya, Koray Dogan, Brooks, Matthew J., Gieser, Linn, Morgan, Nicole Y., Pohida, Tom, Swaroop, Anand
Format:	Journal Article
Language:	English
Published:	United States Elsevier Inc 09.01.2018 Elsevier
Subjects:	3-D organoid culture Animals bioreactor Bioreactors Cell Culture Techniques - instrumentation Cell Culture Techniques - methods embryonic stem cell in vitro organogenesis iPSC Mice Mice, Transgenic Organoids - cytology Organoids - metabolism Pluripotent Stem Cells - cytology Pluripotent Stem Cells - metabolism Resource Retina - cytology Retina - metabolism retina development retinal disease RNA-seq transcriptome retinal disease embryonic stem cell retina development RNA-seq transcriptome iPSC 3-D organoid culture in vitro organogenesis bioreactor
ISSN:	2213-6711, 2213-6711
Online Access:	Get full text
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