Triple-negative vimentin-positive heterogeneous feline mammary carcinomas as a potential comparative model for breast cancer

BACKGROUND: Human breast cancer is a heterogeneous disease classified by molecular subtyping into luminal A, luminal B, HER2-overexpressing, basal-like, claudin-low and normal-breast like. The routinely applied and standardized immunohistochemical-based surrogates of this classification group togeth...

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Vydané v:BMC veterinary research Ročník 10; číslo 1; s. 185
Hlavní autori: Caliari, Diego, Zappulli, Valentina, Rasotto, Roberta, Cardazzo, Barbara, Frassineti, Federica, Goldschmidt, Michael H, Castagnaro, Massimo
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: London Springer-Verlag 25.09.2014
BioMed Central
BioMed Central Ltd
Springer Nature B.V
Predmet:
actin
Animals
Breast cancer
breast neoplasms
Breast Neoplasms - metabolism
Cat Diseases - metabolism
Cats
Comparative analysis
estrogen receptors
Female
gene expression
Gene Expression Regulation, Neoplastic - physiology
Genetic aspects
Humans
immunohistochemistry
Mammary Neoplasms, Animal - metabolism
Medical research
Medicine
Medicine & Public Health
Mortality
Muscle proteins
muscles
Oncology
Physiological aspects
Progesterone
progesterone receptors
Receptors, Cell Surface - genetics
Receptors, Cell Surface - metabolism
Research Article
Rodents
Transgenics
Tumors
Veterinary Medicine/Veterinary Science
vimentin
Vimentin - genetics
Vimentin - metabolism
women
Zoology
Vimentin
Triple-negative
Mammary tumor
Human breast cancer
Feline
Markers
ISSN:1746-6148, 1746-6148
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Shrnutí:BACKGROUND: Human breast cancer is a heterogeneous disease classified by molecular subtyping into luminal A, luminal B, HER2-overexpressing, basal-like, claudin-low and normal-breast like. The routinely applied and standardized immunohistochemical-based surrogates of this classification group together the last three entities as triple-negative breast cancer (TNBCs) that show the most diverse and complex heterogeneity and represent a therapeutic challenge. In the present work 156 feline mammary lesions consisting of feline mammary carcinomas (FMCs), benign neoplasms, and hyperplastic/dysplastic tissues were evaluated histologically and by immunohistochemistry for expression of basal and luminal cytokeratins (CK), vimentin, alpha-smooth muscle actin, calponin, estrogen receptor (ER) alpha (a), and progesterone receptor (PR). Thirty-seven FMCs with 27 matched non-neoplastic controls were also investigated for gene expression of ERa, ER beta, PR, and HER2. RESULTS: A large group of hormone receptors (HRs)-negative aggressive carcinomas - that did not overexpress HER2 - could be distinguished from the less aggressive (10.8%) and benign (8%) HRs + tumors, that showed bilineage (luminal and myoepithelial) differentiation. Immunohistochemical evaluations of cytoplasmic filaments indicated that HRs- FMCs are vimentin+, CK14+, and CK5_6+ carcinomas that may resemble the TNBCs (basal like/claudin low) described in women. The identification of luminal and myoepithelial progenitors within the mammary ductal system suggested potential cells/sites of origin of these tumors. A diffuse and never previously described CKs/vimentin luminal cell co-expression was detected in the non-neoplastic ducts, indicating a potential bilineage progenitor. CONCLUSIONS: These results indicate and potentially explain the high incidence of triple-negative, vimentin + aggressive tumors in cats that may used to elucidate some of the challenging features of TNBCs in women.
Bibliografia:http://dx.doi.org/10.1186/s12917-014-0185-8
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ISSN:1746-6148
1746-6148
DOI:10.1186/s12917-014-0185-8