Rapid identification of Staphylococcus aureus and the mecA gene from BacT/ALERT blood culture bottles by using the LightCycler system

One hundred BacT/ALERT blood culture bottles growing gram-positive cocci in clusters were cultured and studied by LightCycler PCR for the sa442 and mecA genes. PCR was 100% sensitive and specific for detecting Staphylococcus aureus and methicillin resistance in S. aureus but was less accurate for me...

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Bibliographic Details
Published in:Journal of clinical microbiology Vol. 40; no. 7; p. 2659
Main Authors: Shrestha, Nabin K, Tuohy, Marion J, Hall, Gerri S, Isada, Carlos M, Procop, Gary W
Format: Journal Article
Language:English
Published: United States 01.07.2002
Subjects:
Bacteremia - diagnosis
Bacterial Proteins
Bacteriological Techniques - methods
Bacteriological Techniques - statistics & numerical data
Base Sequence
Carrier Proteins - genetics
DNA Primers - genetics
Genes, Bacterial
Hexosyltransferases
Humans
Methicillin Resistance - genetics
Muramoylpentapeptide Carboxypeptidase - genetics
Penicillin-Binding Proteins
Peptidyl Transferases
Polymerase Chain Reaction - methods
Polymerase Chain Reaction - statistics & numerical data
Sensitivity and Specificity
Staphylococcal Infections - diagnosis
Staphylococcus aureus - drug effects
Staphylococcus aureus - genetics
Staphylococcus aureus - isolation & purification
ISSN:0095-1137
Online Access:Get more information
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Summary:One hundred BacT/ALERT blood culture bottles growing gram-positive cocci in clusters were cultured and studied by LightCycler PCR for the sa442 and mecA genes. PCR was 100% sensitive and specific for detecting Staphylococcus aureus and methicillin resistance in S. aureus but was less accurate for methicillin resistance in coagulase-negative staphylococci.
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ISSN:0095-1137
DOI:10.1128/JCM.40.7.2659-2661.2002