Surface Characteristics, Copper Release, and Toxicity of Nano- and Micrometer-Sized Copper and Copper(II) Oxide Particles: A Cross-Disciplinary Study

An interdisciplinary and multianalytical research effort is undertaken to assess the toxic aspects of thoroughly characterized nano‐ and micrometer‐sized particles of oxidized metallic copper and copper(II) oxide in contact with cultivated lung cells, as well as copper release in relevant media. All...

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Bibliographic Details
Published in:Small (Weinheim an der Bergstrasse, Germany) Vol. 5; no. 3; pp. 389 - 399
Main Authors: Midander, Klara, Cronholm, Pontus, Karlsson, Hanna L., Elihn, Karine, Möller, Lennart, Leygraf, Christofer, Wallinder, Inger Odnevall
Format: Journal Article
Language:English
Published: Weinheim WILEY-VCH Verlag 06.02.2009
WILEY‐VCH Verlag
Subjects:
Cell Line
Chemical engineering
Chemical process and manufacturing engineering
copper
Copper - chemistry
Copper - toxicity
cytotoxicity
DNA Damage
Kemisk process- och produktionsteknik
Kemiteknik
Metal Nanoparticles - chemistry
Metal Nanoparticles - toxicity
Metal Nanoparticles - ultrastructure
Microscopy, Electron, Scanning
nanoparticles
particle characterization
Spectrophotometry, Atomic
Surface and colloid chemistry
Surface Properties
TECHNOLOGY
TEKNIKVETENSKAP
Yt- och kolloidkemi
copper
particle characterization
nanoparticles
DNA damage
cytotoxicity
ISSN:1613-6810, 1613-6829, 1613-6829
Online Access:Get full text
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Summary:An interdisciplinary and multianalytical research effort is undertaken to assess the toxic aspects of thoroughly characterized nano‐ and micrometer‐sized particles of oxidized metallic copper and copper(II) oxide in contact with cultivated lung cells, as well as copper release in relevant media. All particles, except micrometer‐sized Cu, release more copper in serum‐containing cell medium (supplemented Dulbecco's minimal essential medium) compared to identical exposures in phosphate‐buffered saline. Sonication of particles for dispersion prior to exposure has a large effect on the initial copper release from Cu nanoparticles. A clear size‐dependent effect is observed from both a copper release and a toxicity perspective. In agreement with greater released amounts of copper per quantity of particles from the nanometer‐sized particles compared to the micrometer‐sized particles, the nanometer particles cause a higher degree of DNA damage (single‐strand breaks) and cause a significantly higher percentage of cell death compared to cytotoxicity induced by micrometer‐sized particles. Cytotoxic effects related to the released copper fraction are found to be significantly lower than the effects related to particles. No DNA damage is induced by the released copper fraction. Save your breath: A multianalytical approach is used to assess the toxicity to cultured lung cells of micro‐ and nanoparticles of oxidized Cu and CuO in relation to the particle characteristics (see picture). Larger amounts of copper per quantity of particles are released from nanoparticles than micrometer‐sized particles. The cytotoxic effects of nanoparticles are caused by the particles rather than the released copper fraction.
Bibliography:istex:DCAFDBC9213DFDEAA4ED9564744E3EFB7C7DBE33
ArticleID:SMLL200801220
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ISSN:1613-6810
1613-6829
1613-6829
DOI:10.1002/smll.200801220