Radiation damage to macromolecules: kill or cure?

Radiation damage induced by X‐ray beams during macromolecular diffraction experiments remains an issue of concern in structural biology. While advances in our understanding of this phenomenon, driven in part by a series of workshops in this area, undoubtedly have been and are still being made, there...

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Vydáno v:	Journal of synchrotron radiation Ročník 22; číslo 2; s. 195 - 200
Hlavní autoři:	Garman, Elspeth F., Weik, Martin
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	5 Abbey Square, Chester, Cheshire CH1 2HU, England International Union of Crystallography 01.03.2015 John Wiley & Sons, Inc
Témata:	Amino acids Beams (radiation) Biochemistry, Molecular Biology Computer Simulation Crystallography, X-Ray Damage Deoxyribonucleic acid Diffraction FEL Humans Life Sciences macromolecular crystallography Macromolecular Substances - radiation effects Macromolecules Models, Molecular Proteins Radiation damage Radiation Injuries radiation damage induced phasing Radioactivity SAXS Scattering, Small Angle Sensitivity and Specificity simulations Structural Biology X-Ray Diffraction - methods X-ray radiation damage X-rays FEL radiation damage induced phasing X-ray radiation damage macromolecular crystallography SAXS simulations
ISSN:	1600-5775, 0909-0495, 1600-5775
On-line přístup:	Získat plný text
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Popis
Shrnutí:	Radiation damage induced by X‐ray beams during macromolecular diffraction experiments remains an issue of concern in structural biology. While advances in our understanding of this phenomenon, driven in part by a series of workshops in this area, undoubtedly have been and are still being made, there are still questions to be answered. Eight papers in this volume give a flavour of ongoing investigations, addressing various issues. These range over: a proposed new metric derived from atomic B‐factors for identifying potentially damaged amino acid residues, a study of the relative damage susceptibility of protein and DNA in a DNA/protein complex, a report of an indication of specific radiation damage to a protein determined from data collected using an X‐ray free‐electron laser (FEL), an account of the challenges in FEL raw diffraction data analysis, an exploration of the possibilities of using radiation damage induced phasing to solve structures using FELs, simulations of radiation damage as a function of FEL temporal pulse profiles, results on the influence of radiation damage during scanning X‐ray diffraction measurements and, lastly, consideration of strategies for minimizing radiation damage during SAXS experiments. In this short introduction, these contributions are briefly placed in the context of other current work on radiation damage in the field.
Bibliografie:	ArticleID:JSY2XH0008 istex:0CC974AE69A136BE43897AFC1F593483050F9B3B ark:/67375/WNG-2WBVTCDD-K ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 ObjectType-Review-3 content type line 23
ISSN:	1600-5775 0909-0495 1600-5775
DOI:	10.1107/S160057751500380X