Identification of autoantibody biomarkers for primary Sjögren's syndrome using protein microarrays

Sjögren's syndrome (SS) is a chronic, progressive autoimmune disease primarily affecting women. Diagnosis of SS requires an invasive salivary gland tissue biopsy and a long delay from the start of the symptoms to final diagnosis has been frequently observed. In this study, we aim to identify sa...

Celý popis

Uloženo v:

Podrobná bibliografie
Vydáno v:	Proteomics (Weinheim) Ročník 11; číslo 8; s. 1499 - 1507
Hlavní autoři:	Hu, Shen, Vissink, Arjan, Arellano, Martha, Roozendaal, Caroline, Zhou, Hui, Kallenberg, Cees G. M., Wong, David T.
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	Weinheim WILEY-VCH Verlag 01.04.2011 WILEY‐VCH Verlag Wiley-VCH Wiley Subscription Services, Inc
Témata:	Analytical, structural and metabolic biochemistry Antibodies, Antinuclear - analysis Autoantibodies - analysis Autoantibodies - immunology Autoantibody biomarker Biological and medical sciences Biomarkers - analysis Enzyme-Linked Immunosorbent Assay Fundamental and applied biological sciences. Psychology Histones - immunology Humans Lupus Erythematosus, Systemic - immunology Medical sciences Miscellaneous Protein Array Analysis Protein arrays Protein microarray Proteins Saliva - immunology Sarcoidosis. Granulomatous diseases of unproved etiology. Connective tissue diseases. Elastic tissue diseases. Vasculitis Sjogren-Larsson Syndrome - diagnosis Sjogren-Larsson Syndrome - immunology Sjogren-Larsson Syndrome - metabolism Sjögren's syndrome Transglutaminases - immunology Autoimmunity Immunopathology Stomatology Autoantibody Biological marker Autoantibody biomarker Autoimmune disease Identification Protein microarray Sjögren syndrome Protein Eye disease Protein arrays Sjögren's syndrome Systemic disease Proteomics
ISSN:	1615-9853, 1615-9861, 1615-9861
On-line přístup:	Získat plný text
Tagy:	Přidat tag Žádné tagy, Buďte první, kdo vytvoří štítek k tomuto záznamu!

Popis
Shrnutí:	Sjögren's syndrome (SS) is a chronic, progressive autoimmune disease primarily affecting women. Diagnosis of SS requires an invasive salivary gland tissue biopsy and a long delay from the start of the symptoms to final diagnosis has been frequently observed. In this study, we aim to identify salivary autoantibody biomarkers for primary SS (pSS) using a protein microarray approach. Immune‐response protoarrays were used to profile saliva autoantibodies from patients with pSS (n=14), patients with systemic lupus erythematosus (SLE, n=13), and healthy control subjects (n=13). We identified 24 potential autoantibody biomarkers that can discriminate patients with pSS from both patients with SLE and healthy individuals. Four saliva autoantibody biomarkers, anti‐transglutaminase, anti‐histone, anti‐SSA, and anti‐SSB, were further tested in independent pSS (n=34), SLE (n=34), and healthy control (n=34) subjects and all were successfully validated with ELISA. This study has demonstrated the potential of a high‐throughput protein microarray approach for the discovery of autoantibody biomarkers. The identified saliva autoantibody biomarkers may lead to a clinical tool for simple, noninvasive detection of pSS at low cost.
Bibliografie:	Colour Online: See the article online to view Fig. 3 in colour PHS - No. R01DE017593 ark:/67375/WNG-QGMSQFTQ-6 istex:07E075540AAC843959C84DFD4BF792D75E5D6F49 ArticleID:PMIC201000206 3 See the article online to view Fig. Colour Online in colour ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 ObjectType-Article-2 ObjectType-Feature-1 Additional corresponding author: Dr. David T. Wong, E-mail: dtww@ucla.edu
ISSN:	1615-9853 1615-9861 1615-9861
DOI:	10.1002/pmic.201000206