Fine Epitope Mapping of the Central Immunodominant Region of Nucleoprotein from Crimean-Congo Hemorrhagic Fever Virus (CCHFV)
Crimean-Congo hemorrhagic fever (CCHF), a severe viral disease known to have occurred in over 30 countries and distinct regions, is caused by the tick-borne CCHF virus (CCHFV). Nucleocapsid protein (NP), which is encoded by the S gene, is the primary antigen detectable in infected cells. The goal of...
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| Vydáno v: | PloS one Ročník 9; číslo 11; s. e108419 |
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Public Library of Science
03.11.2014
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| ISSN: | 1932-6203, 1932-6203 |
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| Abstract | Crimean-Congo hemorrhagic fever (CCHF), a severe viral disease known to have occurred in over 30 countries and distinct regions, is caused by the tick-borne CCHF virus (CCHFV). Nucleocapsid protein (NP), which is encoded by the S gene, is the primary antigen detectable in infected cells. The goal of the present study was to map the minimal motifs of B-cell epitopes (BCEs) on NP. Five precise BCEs (E1, 247FDEAKK252; E2a, 254VEAL257; E2b, 258NGYLNKH264; E3, 267EVDKA271; and E4, 274DSMITN279) identified through the use of rabbit antiserum, and one BCE (E5, 258NGYL261) recognized using a mouse monoclonal antibody, were confirmed to be within the central region of NP and were partially represented among the predicted epitopes. Notably, the five BCEs identified using the rabbit sera were able to react with positive serum mixtures from five sheep which had been infected naturally with CCHFV. The multiple sequence alignment (MSA) revealed high conservation of the identified BCEs among ten CCHFV strains from different areas. Interestingly, the identified BCEs with only one residue variation can apparently be recognized by the positive sera of sheep naturally infected with CCHFV. Computer-generated three-dimensional structural models indicated that all the antigenic motifs are located on the surface of the NP stalk domain. This report represents the first identification and mapping of the minimal BCEs of CCHFV-NP along with an analysis of their primary and structural properties. Our identification of the minimal linear BCEs of CCHFV-NP may provide fundamental data for developing rapid diagnostic reagents and illuminating the pathogenic mechanism of CCHFV. |
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| AbstractList | Crimean-Congo hemorrhagic fever (CCHF), a severe viral disease known to have occurred in over 30 countries and distinct regions, is caused by the tick-borne CCHF virus (CCHFV). Nucleocapsid protein (NP), which is encoded by the S gene, is the primary antigen detectable in infected cells. The goal of the present study was to map the minimal motifs of B-cell epitopes (BCEs) on NP. Five precise BCEs (E1, 247FDEAKK252; E2a, 254VEAL257; E2b, 258NGYLNKH264; E3, 267EVDKA271; and E4, 274DSMITN279) identified through the use of rabbit antiserum, and one BCE (E5, 258NGYL261) recognized using a mouse monoclonal antibody, were confirmed to be within the central region of NP and were partially represented among the predicted epitopes. Notably, the five BCEs identified using the rabbit sera were able to react with positive serum mixtures from five sheep which had been infected naturally with CCHFV. The multiple sequence alignment (MSA) revealed high conservation of the identified BCEs among ten CCHFV strains from different areas. Interestingly, the identified BCEs with only one residue variation can apparently be recognized by the positive sera of sheep naturally infected with CCHFV. Computer-generated three-dimensional structural models indicated that all the antigenic motifs are located on the surface of the NP stalk domain. This report represents the first identification and mapping of the minimal BCEs of CCHFV-NP along with an analysis of their primary and structural properties. Our identification of the minimal linear BCEs of CCHFV-NP may provide fundamental data for developing rapid diagnostic reagents and illuminating the pathogenic mechanism of CCHFV. Crimean-Congo hemorrhagic fever (CCHF), a severe viral disease known to have occurred in over 30 countries and distinct regions, is caused by the tick-borne CCHF virus (CCHFV). Nucleocapsid protein (NP), which is encoded by the S gene, is the primary antigen detectable in infected cells. The goal of the present study was to map the minimal motifs of B-cell epitopes (BCEs) on NP. Five precise BCEs (E1, 247 FDEAKK 252 ; E2a, 254 VEAL 257 ; E2b, 258 NGYLNKH 264 ; E3, 267 EVDKA 271 ; and E4, 274 DSMITN 279 ) identified through the use of rabbit antiserum, and one BCE (E5, 258 NGYL 261 ) recognized using a mouse monoclonal antibody, were confirmed to be within the central region of NP and were partially represented among the predicted epitopes. Notably, the five BCEs identified using the rabbit sera were able to react with positive serum mixtures from five sheep which had been infected naturally with CCHFV. The multiple sequence alignment (MSA) revealed high conservation of the identified BCEs among ten CCHFV strains from different areas. Interestingly, the identified BCEs with only one residue variation can apparently be recognized by the positive sera of sheep naturally infected with CCHFV. Computer-generated three-dimensional structural models indicated that all the antigenic motifs are located on the surface of the NP stalk domain. This report represents the first identification and mapping of the minimal BCEs of CCHFV-NP along with an analysis of their primary and structural properties. Our identification of the minimal linear BCEs of CCHFV-NP may provide fundamental data for developing rapid diagnostic reagents and illuminating the pathogenic mechanism of CCHFV. Crimean-Congo hemorrhagic fever (CCHF), a severe viral disease known to have occurred in over 30 countries and distinct regions, is caused by the tick-borne CCHF virus (CCHFV). Nucleocapsid protein (NP), which is encoded by the S gene, is the primary antigen detectable in infected cells. The goal of the present study was to map the minimal motifs of B-cell epitopes (BCEs) on NP. Five precise BCEs (E1, 247FDEAKK252; E2a, 254VEAL257; E2b, 258NGYLNKH264; E3, 267EVDKA271; and E4, 274DSMITN279) identified through the use of rabbit antiserum, and one BCE (E5, 258NGYL261) recognized using a mouse monoclonal antibody, were confirmed to be within the central region of NP and were partially represented among the predicted epitopes. Notably, the five BCEs identified using the rabbit sera were able to react with positive serum mixtures from five sheep which had been infected naturally with CCHFV. The multiple sequence alignment (MSA) revealed high conservation of the identified BCEs among ten CCHFV strains from different areas. Interestingly, the identified BCEs with only one residue variation can apparently be recognized by the positive sera of sheep naturally infected with CCHFV. Computer-generated three-dimensional structural models indicated that all the antigenic motifs are located on the surface of the NP stalk domain. This report represents the first identification and mapping of the minimal BCEs of CCHFV-NP along with an analysis of their primary and structural properties. Our identification of the minimal linear BCEs of CCHFV-NP may provide fundamental data for developing rapid diagnostic reagents and illuminating the pathogenic mechanism of CCHFV.Crimean-Congo hemorrhagic fever (CCHF), a severe viral disease known to have occurred in over 30 countries and distinct regions, is caused by the tick-borne CCHF virus (CCHFV). Nucleocapsid protein (NP), which is encoded by the S gene, is the primary antigen detectable in infected cells. The goal of the present study was to map the minimal motifs of B-cell epitopes (BCEs) on NP. Five precise BCEs (E1, 247FDEAKK252; E2a, 254VEAL257; E2b, 258NGYLNKH264; E3, 267EVDKA271; and E4, 274DSMITN279) identified through the use of rabbit antiserum, and one BCE (E5, 258NGYL261) recognized using a mouse monoclonal antibody, were confirmed to be within the central region of NP and were partially represented among the predicted epitopes. Notably, the five BCEs identified using the rabbit sera were able to react with positive serum mixtures from five sheep which had been infected naturally with CCHFV. The multiple sequence alignment (MSA) revealed high conservation of the identified BCEs among ten CCHFV strains from different areas. Interestingly, the identified BCEs with only one residue variation can apparently be recognized by the positive sera of sheep naturally infected with CCHFV. Computer-generated three-dimensional structural models indicated that all the antigenic motifs are located on the surface of the NP stalk domain. This report represents the first identification and mapping of the minimal BCEs of CCHFV-NP along with an analysis of their primary and structural properties. Our identification of the minimal linear BCEs of CCHFV-NP may provide fundamental data for developing rapid diagnostic reagents and illuminating the pathogenic mechanism of CCHFV. |
| Author | Kou, Chun Zhang, Fuchun Yang, Jianhua Zhang, Yujiang Liu, Dongliang Deng, Fei Wang, Yongxing Li, Yijie Zhao, Jing Ma, Ji Hu, Zhihong Duan, Xiaomei Sun, Surong Li, Yang Wu, Ting |
| AuthorAffiliation | 2 State Key Laboratory of Virology, Chinese Academy of Sciences, Wuhan, Hubei, China 1 Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi, Xinjiang, China 3 Center for Disease Control and Prevention of the Xinjiang Uyghur Autonomous Region, Urumqi, Xinjiang, China 4 Texas Children's Cancer Center, Department of Pediatrics, Dan L. Duncan Cancer Center, Baylor College of Medicine, Houston, Texas, United States of America Division of Clinical Research, United States of America |
| AuthorAffiliation_xml | – name: 4 Texas Children's Cancer Center, Department of Pediatrics, Dan L. Duncan Cancer Center, Baylor College of Medicine, Houston, Texas, United States of America – name: 1 Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi, Xinjiang, China – name: 3 Center for Disease Control and Prevention of the Xinjiang Uyghur Autonomous Region, Urumqi, Xinjiang, China – name: 2 State Key Laboratory of Virology, Chinese Academy of Sciences, Wuhan, Hubei, China – name: Division of Clinical Research, United States of America |
| Author_xml | – sequence: 1 givenname: Dongliang surname: Liu fullname: Liu, Dongliang – sequence: 2 givenname: Yang surname: Li fullname: Li, Yang – sequence: 3 givenname: Jing surname: Zhao fullname: Zhao, Jing – sequence: 4 givenname: Fei surname: Deng fullname: Deng, Fei – sequence: 5 givenname: Xiaomei surname: Duan fullname: Duan, Xiaomei – sequence: 6 givenname: Chun surname: Kou fullname: Kou, Chun – sequence: 7 givenname: Ting surname: Wu fullname: Wu, Ting – sequence: 8 givenname: Yijie surname: Li fullname: Li, Yijie – sequence: 9 givenname: Yongxing surname: Wang fullname: Wang, Yongxing – sequence: 10 givenname: Ji surname: Ma fullname: Ma, Ji – sequence: 11 givenname: Jianhua surname: Yang fullname: Yang, Jianhua – sequence: 12 givenname: Zhihong surname: Hu fullname: Hu, Zhihong – sequence: 13 givenname: Fuchun surname: Zhang fullname: Zhang, Fuchun – sequence: 14 givenname: Yujiang surname: Zhang fullname: Zhang, Yujiang – sequence: 15 givenname: Surong surname: Sun fullname: Sun, Surong |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/25365026$$D View this record in MEDLINE/PubMed |
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| Copyright | 2014 Liu et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. 2014 Liu et al 2014 Liu et al |
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| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 Conceived and designed the experiments: SRS DLL YJZ FCZ. Performed the experiments: DLL YL JZ XMD CK TW. Analyzed the data: SRS DLL YJL JM YXW. Contributed reagents/materials/analysis tools: DLL SRS YJZ ZHH FD. Wrote the paper: DLL SRS JHY YJZ ZHH. Competing Interests: The authors have declared that no competing interests exist. |
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| Snippet | Crimean-Congo hemorrhagic fever (CCHF), a severe viral disease known to have occurred in over 30 countries and distinct regions, is caused by the tick-borne... |
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| SubjectTerms | Amino Acid Sequence Animal models Animals Antibodies - blood Antibodies - immunology Antigens Biology and Life Sciences Cancer Conservation Conserved Sequence Crimean hemorrhagic fever Diagnostic systems Epitope Mapping Epitopes, B-Lymphocyte - chemistry Epitopes, B-Lymphocyte - immunology Fever Gene Expression Genetic engineering Genomes Hemorrhage Hemorrhagic Fever Virus, Crimean-Congo - genetics Hemorrhagic Fever Virus, Crimean-Congo - immunology Hemorrhagic Fever, Crimean - immunology Humans Immunodominant Epitopes - chemistry Immunodominant Epitopes - genetics Immunodominant Epitopes - immunology Immunoglobulins Laboratories Life sciences Lymphocytes B Mapping Models, Molecular Molecular Sequence Data Monoclonal antibodies Nosocomial infections Nucleocapsids Nucleoproteins - chemistry Nucleoproteins - genetics Nucleoproteins - immunology Nucleotide sequence Peptide Fragments - chemistry Peptide Fragments - genetics Peptide Fragments - immunology Peptides Protein Conformation Proteins Reagents Recombinant Fusion Proteins - chemistry Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - immunology S gene Sequence Alignment Sheep Structural models Studies Three dimensional models Viral diseases Virology Viruses |
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| Title | Fine Epitope Mapping of the Central Immunodominant Region of Nucleoprotein from Crimean-Congo Hemorrhagic Fever Virus (CCHFV) |
| URI | https://www.ncbi.nlm.nih.gov/pubmed/25365026 https://www.proquest.com/docview/1619502529 https://www.proquest.com/docview/1620587375 https://pubmed.ncbi.nlm.nih.gov/PMC4217714 https://doaj.org/article/51e633136c8e4c8f92ad4d28b0c44472 http://dx.doi.org/10.1371/journal.pone.0108419 |
| Volume | 9 |
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