Fine Epitope Mapping of the Central Immunodominant Region of Nucleoprotein from Crimean-Congo Hemorrhagic Fever Virus (CCHFV)
Crimean-Congo hemorrhagic fever (CCHF), a severe viral disease known to have occurred in over 30 countries and distinct regions, is caused by the tick-borne CCHF virus (CCHFV). Nucleocapsid protein (NP), which is encoded by the S gene, is the primary antigen detectable in infected cells. The goal of...
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| Abstract | Crimean-Congo hemorrhagic fever (CCHF), a severe viral disease known to have occurred in over 30 countries and distinct regions, is caused by the tick-borne CCHF virus (CCHFV). Nucleocapsid protein (NP), which is encoded by the S gene, is the primary antigen detectable in infected cells. The goal of the present study was to map the minimal motifs of B-cell epitopes (BCEs) on NP. Five precise BCEs (E1, 247FDEAKK252; E2a, 254VEAL257; E2b, 258NGYLNKH264; E3, 267EVDKA271; and E4, 274DSMITN279) identified through the use of rabbit antiserum, and one BCE (E5, 258NGYL261) recognized using a mouse monoclonal antibody, were confirmed to be within the central region of NP and were partially represented among the predicted epitopes. Notably, the five BCEs identified using the rabbit sera were able to react with positive serum mixtures from five sheep which had been infected naturally with CCHFV. The multiple sequence alignment (MSA) revealed high conservation of the identified BCEs among ten CCHFV strains from different areas. Interestingly, the identified BCEs with only one residue variation can apparently be recognized by the positive sera of sheep naturally infected with CCHFV. Computer-generated three-dimensional structural models indicated that all the antigenic motifs are located on the surface of the NP stalk domain. This report represents the first identification and mapping of the minimal BCEs of CCHFV-NP along with an analysis of their primary and structural properties. Our identification of the minimal linear BCEs of CCHFV-NP may provide fundamental data for developing rapid diagnostic reagents and illuminating the pathogenic mechanism of CCHFV. |
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| AbstractList | Crimean-Congo hemorrhagic fever (CCHF), a severe viral disease known to have occurred in over 30 countries and distinct regions, is caused by the tick-borne CCHF virus (CCHFV). Nucleocapsid protein (NP), which is encoded by the S gene, is the primary antigen detectable in infected cells. The goal of the present study was to map the minimal motifs of B-cell epitopes (BCEs) on NP. Five precise BCEs (E1, 247FDEAKK252; E2a, 254VEAL257; E2b, 258NGYLNKH264; E3, 267EVDKA271; and E4, 274DSMITN279) identified through the use of rabbit antiserum, and one BCE (E5, 258NGYL261) recognized using a mouse monoclonal antibody, were confirmed to be within the central region of NP and were partially represented among the predicted epitopes. Notably, the five BCEs identified using the rabbit sera were able to react with positive serum mixtures from five sheep which had been infected naturally with CCHFV. The multiple sequence alignment (MSA) revealed high conservation of the identified BCEs among ten CCHFV strains from different areas. Interestingly, the identified BCEs with only one residue variation can apparently be recognized by the positive sera of sheep naturally infected with CCHFV. Computer-generated three-dimensional structural models indicated that all the antigenic motifs are located on the surface of the NP stalk domain. This report represents the first identification and mapping of the minimal BCEs of CCHFV-NP along with an analysis of their primary and structural properties. Our identification of the minimal linear BCEs of CCHFV-NP may provide fundamental data for developing rapid diagnostic reagents and illuminating the pathogenic mechanism of CCHFV. Crimean-Congo hemorrhagic fever (CCHF), a severe viral disease known to have occurred in over 30 countries and distinct regions, is caused by the tick-borne CCHF virus (CCHFV). Nucleocapsid protein (NP), which is encoded by the S gene, is the primary antigen detectable in infected cells. The goal of the present study was to map the minimal motifs of B-cell epitopes (BCEs) on NP. Five precise BCEs (E1, 247FDEAKK252; E2a, 254VEAL257; E2b, 258NGYLNKH264; E3, 267EVDKA271; and E4, 274DSMITN279) identified through the use of rabbit antiserum, and one BCE (E5, 258NGYL261) recognized using a mouse monoclonal antibody, were confirmed to be within the central region of NP and were partially represented among the predicted epitopes. Notably, the five BCEs identified using the rabbit sera were able to react with positive serum mixtures from five sheep which had been infected naturally with CCHFV. The multiple sequence alignment (MSA) revealed high conservation of the identified BCEs among ten CCHFV strains from different areas. Interestingly, the identified BCEs with only one residue variation can apparently be recognized by the positive sera of sheep naturally infected with CCHFV. Computer-generated three-dimensional structural models indicated that all the antigenic motifs are located on the surface of the NP stalk domain. This report represents the first identification and mapping of the minimal BCEs of CCHFV-NP along with an analysis of their primary and structural properties. Our identification of the minimal linear BCEs of CCHFV-NP may provide fundamental data for developing rapid diagnostic reagents and illuminating the pathogenic mechanism of CCHFV.Crimean-Congo hemorrhagic fever (CCHF), a severe viral disease known to have occurred in over 30 countries and distinct regions, is caused by the tick-borne CCHF virus (CCHFV). Nucleocapsid protein (NP), which is encoded by the S gene, is the primary antigen detectable in infected cells. The goal of the present study was to map the minimal motifs of B-cell epitopes (BCEs) on NP. Five precise BCEs (E1, 247FDEAKK252; E2a, 254VEAL257; E2b, 258NGYLNKH264; E3, 267EVDKA271; and E4, 274DSMITN279) identified through the use of rabbit antiserum, and one BCE (E5, 258NGYL261) recognized using a mouse monoclonal antibody, were confirmed to be within the central region of NP and were partially represented among the predicted epitopes. Notably, the five BCEs identified using the rabbit sera were able to react with positive serum mixtures from five sheep which had been infected naturally with CCHFV. The multiple sequence alignment (MSA) revealed high conservation of the identified BCEs among ten CCHFV strains from different areas. Interestingly, the identified BCEs with only one residue variation can apparently be recognized by the positive sera of sheep naturally infected with CCHFV. Computer-generated three-dimensional structural models indicated that all the antigenic motifs are located on the surface of the NP stalk domain. This report represents the first identification and mapping of the minimal BCEs of CCHFV-NP along with an analysis of their primary and structural properties. Our identification of the minimal linear BCEs of CCHFV-NP may provide fundamental data for developing rapid diagnostic reagents and illuminating the pathogenic mechanism of CCHFV. Crimean-Congo hemorrhagic fever (CCHF), a severe viral disease known to have occurred in over 30 countries and distinct regions, is caused by the tick-borne CCHF virus (CCHFV). Nucleocapsid protein (NP), which is encoded by the S gene, is the primary antigen detectable in infected cells. The goal of the present study was to map the minimal motifs of B-cell epitopes (BCEs) on NP. Five precise BCEs (E1, 247 FDEAKK 252 ; E2a, 254 VEAL 257 ; E2b, 258 NGYLNKH 264 ; E3, 267 EVDKA 271 ; and E4, 274 DSMITN 279 ) identified through the use of rabbit antiserum, and one BCE (E5, 258 NGYL 261 ) recognized using a mouse monoclonal antibody, were confirmed to be within the central region of NP and were partially represented among the predicted epitopes. Notably, the five BCEs identified using the rabbit sera were able to react with positive serum mixtures from five sheep which had been infected naturally with CCHFV. The multiple sequence alignment (MSA) revealed high conservation of the identified BCEs among ten CCHFV strains from different areas. Interestingly, the identified BCEs with only one residue variation can apparently be recognized by the positive sera of sheep naturally infected with CCHFV. Computer-generated three-dimensional structural models indicated that all the antigenic motifs are located on the surface of the NP stalk domain. This report represents the first identification and mapping of the minimal BCEs of CCHFV-NP along with an analysis of their primary and structural properties. Our identification of the minimal linear BCEs of CCHFV-NP may provide fundamental data for developing rapid diagnostic reagents and illuminating the pathogenic mechanism of CCHFV. |
| Author | Kou, Chun Zhang, Fuchun Yang, Jianhua Zhang, Yujiang Liu, Dongliang Deng, Fei Wang, Yongxing Li, Yijie Zhao, Jing Ma, Ji Hu, Zhihong Duan, Xiaomei Sun, Surong Li, Yang Wu, Ting |
| AuthorAffiliation | 2 State Key Laboratory of Virology, Chinese Academy of Sciences, Wuhan, Hubei, China 1 Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi, Xinjiang, China 3 Center for Disease Control and Prevention of the Xinjiang Uyghur Autonomous Region, Urumqi, Xinjiang, China 4 Texas Children's Cancer Center, Department of Pediatrics, Dan L. Duncan Cancer Center, Baylor College of Medicine, Houston, Texas, United States of America Division of Clinical Research, United States of America |
| AuthorAffiliation_xml | – name: 4 Texas Children's Cancer Center, Department of Pediatrics, Dan L. Duncan Cancer Center, Baylor College of Medicine, Houston, Texas, United States of America – name: 1 Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi, Xinjiang, China – name: 3 Center for Disease Control and Prevention of the Xinjiang Uyghur Autonomous Region, Urumqi, Xinjiang, China – name: 2 State Key Laboratory of Virology, Chinese Academy of Sciences, Wuhan, Hubei, China – name: Division of Clinical Research, United States of America |
| Author_xml | – sequence: 1 givenname: Dongliang surname: Liu fullname: Liu, Dongliang – sequence: 2 givenname: Yang surname: Li fullname: Li, Yang – sequence: 3 givenname: Jing surname: Zhao fullname: Zhao, Jing – sequence: 4 givenname: Fei surname: Deng fullname: Deng, Fei – sequence: 5 givenname: Xiaomei surname: Duan fullname: Duan, Xiaomei – sequence: 6 givenname: Chun surname: Kou fullname: Kou, Chun – sequence: 7 givenname: Ting surname: Wu fullname: Wu, Ting – sequence: 8 givenname: Yijie surname: Li fullname: Li, Yijie – sequence: 9 givenname: Yongxing surname: Wang fullname: Wang, Yongxing – sequence: 10 givenname: Ji surname: Ma fullname: Ma, Ji – sequence: 11 givenname: Jianhua surname: Yang fullname: Yang, Jianhua – sequence: 12 givenname: Zhihong surname: Hu fullname: Hu, Zhihong – sequence: 13 givenname: Fuchun surname: Zhang fullname: Zhang, Fuchun – sequence: 14 givenname: Yujiang surname: Zhang fullname: Zhang, Yujiang – sequence: 15 givenname: Surong surname: Sun fullname: Sun, Surong |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/25365026$$D View this record in MEDLINE/PubMed |
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| Cites_doi | 10.1128/JVI.00752-06 10.1128/JVI.01859-09 10.1128/JVI.78.8.4323-4329.2004 10.1093/jmedent/15.4.307 10.1128/CDLI.10.3.489-491.2003 10.1074/jbc.M110.149369 10.1007/978-1-4613-1571-1_10 10.1016/0022-2836(82)90515-0 10.1073/pnas.1200808109 10.1073/pnas.89.6.2429 10.1002/jmv.1890460402 10.1016/j.tmaid.2010.04.008 10.1002/(SICI)1096-9071(199606)49:2<83::AID-JMV2>3.0.CO;2-F 10.1099/0022-1317-71-3-501 10.1007/BF01195768 10.1007/s12250-010-3067-7 10.1038/227680a0 10.1016/j.jviromet.2011.11.020 10.1016/S0166-3542(04)00163-9 10.1016/0022-1759(89)90402-X 10.1016/j.vetmic.2009.07.011 10.1128/JVI.55.3.836-839.1985 10.1016/j.jcv.2005.02.016 10.1007/978-1-61779-974-7_37 10.1093/nar/gkg500 10.1016/j.cbpa.2005.06.003 10.4269/ajtmh.1987.36.120 10.1002/jmv.20270 10.1016/j.jviromet.2011.09.023 10.1111/j.1600-0897.2012.01177.x 10.1089/088282402317340323 10.1016/j.ijid.2008.08.002 10.1016/S1473-3099(06)70435-2 10.1002/9780470122778.ch6 10.1128/JVI.01555-12 10.1128/genomeA.00571-13 10.1073/pnas.87.16.6378 10.1007/978-3-540-73259-4_15 10.1080/02648725.2007.10648092 10.1073/pnas.71.5.1743 10.1073/pnas.76.9.4350 10.1073/pnas.97.22.12266 10.1016/j.jviromet.2013.07.055 10.1128/JVI.01627-12 10.1099/vir.0.035105-0 10.3201/eid0801.010087 10.1128/JCM.40.5.1587-1591.2002 10.1016/j.jri.2009.04.004 |
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| Copyright | 2014 Liu et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. 2014 Liu et al 2014 Liu et al |
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| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 Conceived and designed the experiments: SRS DLL YJZ FCZ. Performed the experiments: DLL YL JZ XMD CK TW. Analyzed the data: SRS DLL YJL JM YXW. Contributed reagents/materials/analysis tools: DLL SRS YJZ ZHH FD. Wrote the paper: DLL SRS JHY YJZ ZHH. Competing Interests: The authors have declared that no competing interests exist. |
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| References | FJ Burt (ref48) 2013; 193 HC Maltezou (ref6) 2010; 8 S Garcia (ref20) 2006; 35 I Andersson (ref50) 2004; 78 J Kyte (ref36) 1982; 157 Z Zhou (ref41) 2013; 1 H Towbin (ref43) 1979; 76 TF Schwarz (ref15) 1996; 49 Q Tang (ref18) 2003; 10 BA Jameson (ref39) 1988; 4 M Saijo (ref19) 2005; 75 JP Kints (ref32) 1989; 119 F Magurano (ref14) 1999; 6 AS Lo (ref52) 2008; 181 H Karlberg (ref56) 2011; 286 RR Samudzi (ref47) 2012; 179 M Saijo (ref21) 2002; 40 SE Cwirla (ref25) 1990; 87 ref45 VM Deyde (ref4) 2006; 80 R Chenna (ref44) 2003; 13 Y Wang (ref57) 2012; 86 M Stocks (ref54) 2005; 9 EA Emini (ref38) 1985; 55 É Bergeron (ref11) 2010; 84 BL Roberts (ref26) 1992; 89 Ö Ergönül (ref2) 2006; 6 CT Walter (ref13) 2011; 92 E Tse (ref55) 2000; 97 RM Elliott (ref1) 1990; 71 ref3 RB Merrifield (ref24) 1968; 32 WX Xu (ref28) 2009; 81 S Moutel (ref53) 2012; 907 X Dai (ref33) 2006; 27 SD Carter (ref58) 2012; 86 ref34 Y Guo (ref59) 2012; 109 SR Sun (ref10) 2009; 47 ref30 PF Wei (ref22) 2010; 25 O Vapalahti (ref16) 1995; 46 JF Morrow (ref23) 1974; 71 ZW Zhang (ref40) 2010; 140 H Hoogstraal (ref5) 1979; 15 R Swanepoel (ref7) 1987; 36 S Chinikar (ref8) 2009; 1 CA Whitehouse (ref12) 2004; 64 PY Chou (ref35) 1978; 47 RC Ladner (ref27) 2007; 24 A Papa (ref49) 2002; 8 PA Karplus (ref37) 1985; 72 UK Laemmli (ref42) 1970; 227 WX Xu (ref29) 2012; 68 Å Lundkvist (ref46) 2002; 1 SD Dowall (ref17) 2012; 179 Y Gürbüz (ref9) 2009; 13 P Srinivasan (ref51) 2011; 2 DL Liu (ref31) 2013; 29 18071953 - Handb Exp Pharmacol. 2008;(181):343-73 18948048 - Int J Infect Dis. 2009 May;13(3):e105-7 388439 - Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4 11050246 - Proc Natl Acad Sci U S A. 2000 Oct 24;97(22):12266-71 21865443 - J Gen Virol. 2011 Nov;92(Pt 11):2467-84 17415983 - Zhonghua Liu Xing Bing Xue Za Zhi. 2006 Dec;27(12):1048-52 8991940 - J Med Virol. 1996 Jun;49(2):83-6 2179464 - J Gen Virol. 1990 Mar;71 ( Pt 3):501-22 20541133 - Travel Med Infect Dis. 2010 May;8(3):139-43 9874664 - Clin Diagn Lab Immunol. 1999 Jan;6(1):55-60 21123175 - J Biol Chem. 2011 Feb 4;286(5):3227-34 22875964 - J Virol. 2012 Oct;86(20):10914-23 22303367 - Front Genet. 2011 Nov 02;2:72 364941 - Adv Enzymol Relat Areas Mol Biol. 1978;47:45-148 11749748 - Emerg Infect Dis. 2002 Jan;8(1):50-3 11980926 - J Clin Microbiol. 2002 May;40(5):1587-91 2723442 - J Immunol Methods. 1989 May 12;119(2):241-5 4307033 - Adv Enzymol Relat Areas Mol Biol. 1969;32:221-96 20960283 - Virol Sin. 2010 Feb;25(1):45-51 22907379 - Methods Mol Biol. 2012;907:667-79 22162720 - Clin Dev Immunol. 2012;2012:831010 5432063 - Nature. 1970 Aug 15;227(5259):680-5 19553586 - J Clin Microbiol. 2009 Aug;47(8):2536-43 4600264 - Proc Natl Acad Sci U S A. 1974 May;71(5):1743-7 22421137 - Proc Natl Acad Sci U S A. 2012 Mar 27;109(13):5046-51 19539378 - J Reprod Immunol. 2009 Jul;81(1):9-16 15047845 - J Virol. 2004 Apr;78(8):4323-9 15550268 - Antiviral Res. 2004 Dec;64(3):145-60 23908296 - Genome Announc. 2013 Aug 01;1(4):null 2991600 - J Virol. 1985 Sep;55(3):836-9 23948410 - Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2013 Aug;29(8):838-41 7595404 - J Med Virol. 1995 Aug;46(4):293-303 2454713 - Comput Appl Biosci. 1988 Mar;4(1):181-6 15979379 - Curr Opin Chem Biol. 2005 Aug;9(4):359-65 16912331 - J Virol. 2006 Sep;80(17):8834-42 18059626 - Biotechnol Genet Eng Rev. 2007;24:1-30 22001274 - J Virol Methods. 2012 Jan;179(1):70-6 19864393 - J Virol. 2010 Jan;84(1):216-26 22155577 - J Virol Methods. 2012 Feb;179(2):335-41 22860757 - Am J Reprod Immunol. 2012 Dec;68(6):465-75 1549606 - Proc Natl Acad Sci U S A. 1992 Mar 15;89(6):2429-33 11952140 - Viral Immunol. 2002;15(1):177-92 22951837 - J Virol. 2012 Nov;86(22):12294-303 15602720 - J Med Virol. 2005 Feb;75(2):295-9 3101525 - Am J Trop Med Hyg. 1987 Jan;36(1):120-32 19699594 - Vet Microbiol. 2010 Jan 6;140(1-2):25-33 16554245 - Lancet Infect Dis. 2006 Apr;6(4):203-14 23933073 - J Virol Methods. 2013 Nov;193(2):706-12 113533 - J Med Entomol. 1979 May 22;15(4):307-417 12824352 - Nucleic Acids Res. 2003 Jul 1;31(13):3497-500 2201029 - Proc Natl Acad Sci U S A. 1990 Aug;87(16):6378-82 7108955 - J Mol Biol. 1982 May 5;157(1):105-32 12738657 - Clin Diagn Lab Immunol. 2003 May;10(3):489-91 16087395 - J Clin Virol. 2006 Feb;35(2):154-9 |
| References_xml | – volume: 80 start-page: 8834 year: 2006 ident: ref4 article-title: Crimean-Congo hemorrhagic fever virus genomics and global diversity publication-title: J Virol doi: 10.1128/JVI.00752-06 – volume: 84 start-page: 216 year: 2010 ident: ref11 article-title: Crimean-Congo hemorrhagic fever virus-encoded ovarian tumor protease activity is dispensable for virus RNA polymerase function publication-title: J Virol doi: 10.1128/JVI.01859-09 – volume: 78 start-page: 4323 year: 2004 ident: ref50 article-title: Human MxA protein inhibits the replication of Crimean-Congo hemorrhagic fever virus publication-title: J Virol doi: 10.1128/JVI.78.8.4323-4329.2004 – volume: 15 start-page: 307 year: 1979 ident: ref5 article-title: The epidemiology of tick-borne Crimean-Congo hemorrhagic fever in Asia, Europe, and Africa publication-title: J Med Entomol doi: 10.1093/jmedent/15.4.307 – volume: 10 start-page: 489 year: 2003 ident: ref18 article-title: A patient with Crimean-Congo hemorrhagic fever serologically diagnosed by recombinant nucleoprotein-based antibody detection systems publication-title: Clin Vaccine Immunol doi: 10.1128/CDLI.10.3.489-491.2003 – volume: 286 start-page: 3227 year: 2011 ident: ref56 article-title: Induction of caspase activation and cleavage of the viral nucleocapsid protein in different cell types during Crimean-Congo hemorrhagic fever virus infection publication-title: J Biol Chem doi: 10.1074/jbc.M110.149369 – ident: ref34 doi: 10.1007/978-1-4613-1571-1_10 – volume: 157 start-page: 105 year: 1982 ident: ref36 article-title: A simple method for displaying the hydropathic character of a protein publication-title: J Mol Biol doi: 10.1016/0022-2836(82)90515-0 – volume: 109 start-page: 5046 year: 2012 ident: ref59 article-title: Crimean-Congo hemorrhagic fever virus nucleoprotein reveals endonuclease activity in bunyaviruses publication-title: Proc Natl Acad Sci doi: 10.1073/pnas.1200808109 – volume: 89 start-page: 2429 year: 1992 ident: ref26 article-title: Directed evolution of a protein: selection of potent neutrophil elastase inhibitors displayed on M13 fusion phage publication-title: Proc Natl Acad Sci doi: 10.1073/pnas.89.6.2429 – volume: 29 start-page: 838 year: 2013 ident: ref31 article-title: Preparation of polyclonal antibody to nucleoprotein from Xinjiang hemorrhagic fever virus and its immunological evaluation publication-title: Chinese J Cell Mol Immunol – volume: 46 start-page: 293 year: 1995 ident: ref16 article-title: Human B-cell epitopes of puumala virus nucleocapsid protein, the major antigen in early serological response publication-title: J Med Virol doi: 10.1002/jmv.1890460402 – volume: 1 start-page: 7 year: 2009 ident: ref8 article-title: An overview of Crimean-Congo hemorrhagic fever in Iran. Iran publication-title: J Microbiol – volume: 8 start-page: 139 year: 2010 ident: ref6 article-title: Crimean–Congo hemorrhagic fever: Risk for emergence of new endemic foci in Europe? publication-title: Travel Med Infect Di doi: 10.1016/j.tmaid.2010.04.008 – volume: 49 start-page: 83 year: 1996 ident: ref15 article-title: Immunoblot detection of antibodies to Toscana virus publication-title: J Med Virol doi: 10.1002/(SICI)1096-9071(199606)49:2<83::AID-JMV2>3.0.CO;2-F – ident: ref30 – volume: 71 start-page: 501 year: 1990 ident: ref1 article-title: Molecular biology of the Bunyaviridae publication-title: J Gen Virol doi: 10.1099/0022-1317-71-3-501 – volume: 72 start-page: 212 year: 1985 ident: ref37 article-title: Prediction of chain flexibility in proteins publication-title: Naturwissenschaften doi: 10.1007/BF01195768 – volume: 25 start-page: 45 year: 2010 ident: ref22 article-title: Serial expression of the.truncated fragments of the nucleocapsid protein of CCHFV and identification of the epitope region publication-title: Virol Sin doi: 10.1007/s12250-010-3067-7 – volume: 27 start-page: 1048 year: 2006 ident: ref33 article-title: Geography and host distribution of Crimean - Congo hemorrhagic fever in the Tarim Basin publication-title: Chin J Epidemiol – volume: 227 start-page: 680 year: 1970 ident: ref42 article-title: Cleavage of structural proteins during the assembly of the head of bacteriophage T4 publication-title: Nature doi: 10.1038/227680a0 – volume: 179 start-page: 335 year: 2012 ident: ref17 article-title: Development of an indirect ELISA method for the parallel measurement of IgG and IgM antibodies against Crimean-Congo haemorrhagic fever (CCHF) virus using recombinant nucleoprotein as antigen publication-title: J Virol Methods doi: 10.1016/j.jviromet.2011.11.020 – volume: 64 start-page: 145 year: 2004 ident: ref12 article-title: Crimean–Congo hemorrhagic fever publication-title: Antivir Res doi: 10.1016/S0166-3542(04)00163-9 – volume: 119 start-page: 241 year: 1989 ident: ref32 article-title: Rat monoclonal antibodies VII. Enhancement of ascites production and yield of monoclonal antibodies in rats following pretreatment with pristane and Freund's adjuvant publication-title: J Immunol Methods doi: 10.1016/0022-1759(89)90402-X – volume: 140 start-page: 25 year: 2010 ident: ref40 article-title: Screening and identification of B cell epitopes of structural proteins of foot-and-mouth disease virus serotype Asia1 publication-title: Vet Microbiol doi: 10.1016/j.vetmic.2009.07.011 – volume: 55 start-page: 836 year: 1985 ident: ref38 article-title: Induction of hepatitis A virus-neutralizing antibody by a virus-specific synthetic peptide publication-title: J Virol doi: 10.1128/JVI.55.3.836-839.1985 – volume: 35 start-page: 154 year: 2006 ident: ref20 article-title: Evaluation of a Crimean-Congo hemorrhagic fever virus recombinant antigen expressed by Semliki Forest suicide virus for IgM and IgG antibody detection in human and animal sera collected in Iran publication-title: J Clin Virol doi: 10.1016/j.jcv.2005.02.016 – volume: 907 start-page: 667 year: 2012 ident: ref53 article-title: Selection and Use of Intracellular Antibodies (Intrabodies) publication-title: Methods Mol Biol doi: 10.1007/978-1-61779-974-7_37 – ident: ref3 – volume: 13 start-page: 3497 year: 2003 ident: ref44 article-title: Multiple sequence alignment with the Clustal series of programs publication-title: Nucleic Acids Res doi: 10.1093/nar/gkg500 – ident: ref45 – volume: 9 start-page: 359 year: 2005 ident: ref54 article-title: Intrabodies as drug discovery tools and therapeutics publication-title: Curr Opin Chem Biol doi: 10.1016/j.cbpa.2005.06.003 – volume: 36 start-page: 120 year: 1987 ident: ref7 article-title: Epidemiologic and clinical features of Crimean-Congo hemorrhagic fever in southern Africa publication-title: Am J Trop Med Hyg doi: 10.4269/ajtmh.1987.36.120 – volume: 47 start-page: 2536 year: 2009 ident: ref10 article-title: J Clin Microbiol – volume: 75 start-page: 295 year: 2005 ident: ref19 article-title: Recombinant nucleoprotein-based serological diagnosis of Crimean-Congo hemorrhagic fever virus infections publication-title: J Med Virol doi: 10.1002/jmv.20270 – volume: 179 start-page: 70 year: 2012 ident: ref47 article-title: Bacterial expression of Crimean-Congo hemorrhagic fever virus nucleoprotein and its evaluation as a diagnostic reagent in an indirect ELISA publication-title: J Virol Methods doi: 10.1016/j.jviromet.2011.09.023 – volume: 68 start-page: 465 year: 2012 ident: ref29 article-title: Mapping of Epitopes Relevant for Induction of Acrosome Reaction on Human Zona Pellucida Glycoprotein-4 Using Monoclonal Antibodies. Am publication-title: J Reprod Immunol doi: 10.1111/j.1600-0897.2012.01177.x – volume: 1 start-page: 177 year: 2002 ident: ref46 article-title: Mapping of B-cell epitopes in the nucleocapsid protein of Puumala hantavirus publication-title: Viral Immunol doi: 10.1089/088282402317340323 – volume: 2 start-page: 72 year: 2011 ident: ref51 article-title: Epitope-based immunoinformatics and molecular docking studies of nucleocapsid protein and ovarian tumor domain of Crimean-Congo hemorrhagic fever virus publication-title: Front Gen – volume: 13 start-page: e105 year: 2009 ident: ref9 article-title: A case of nosocomial transmission of Crimean–Congo hemorrhagic fever from patient to patient publication-title: Int J Infect Dis doi: 10.1016/j.ijid.2008.08.002 – volume: 6 start-page: 203 year: 2006 ident: ref2 article-title: Crimean-Congo haemorrhagic fever publication-title: Lancet Infect Dis doi: 10.1016/S1473-3099(06)70435-2 – volume: 6 start-page: 55 year: 1999 ident: ref14 article-title: Humoral response in Toscana virus acute neurologic disease investigated by viral-protein-specific immunoassays publication-title: Clin Vaccine Immunol – volume: 32 start-page: 221 year: 1968 ident: ref24 article-title: Solid-phase peptide synthesis publication-title: Adv Enzymol Relat Areas Mol Biol doi: 10.1002/9780470122778.ch6 – volume: 47 start-page: 45 year: 1978 ident: ref35 article-title: Prediction of the secondary structure of proteins from their amino acid sequence publication-title: Adv Enzymol Relat Areas Mol Biol – volume: 86 start-page: 10914 year: 2012 ident: ref58 article-title: Structure, function, and evolution of the Crimean-Congo hemorrhagic fever virus nucleocapsid protein publication-title: J Virol doi: 10.1128/JVI.01555-12 – volume: 1 start-page: e00571 year: 2013 ident: ref41 article-title: Complete genome sequences of two Crimean-Congo hemorrhagic fever viruses isolated in China publication-title: Genome Announc doi: 10.1128/genomeA.00571-13 – volume: 4 start-page: 181 year: 1988 ident: ref39 article-title: The antigenic index: a novel algorithm for predicting antigenic determinants publication-title: Comput Appl Biosci – volume: 87 start-page: 6378 year: 1990 ident: ref25 article-title: Peptides on phage: a vast library of peptides for identifying ligands publication-title: Proc Natl Acad Sci doi: 10.1073/pnas.87.16.6378 – volume: 181 start-page: 343 year: 2008 ident: ref52 article-title: Intracellular antibodies (intrabodies) and their therapeutic potential publication-title: Handb Exp Pharmacol doi: 10.1007/978-3-540-73259-4_15 – volume: 24 start-page: 1 year: 2007 ident: ref27 article-title: Mapping the epitopes of antibodies publication-title: Biotechnol Genet Eng doi: 10.1080/02648725.2007.10648092 – volume: 71 start-page: 1743 year: 1974 ident: ref23 article-title: Replication and Transcription of Eukaryotic DNA in Esherichia coli publication-title: Proc Natl Acad Sci doi: 10.1073/pnas.71.5.1743 – volume: 76 start-page: 4350 year: 1979 ident: ref43 article-title: Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications publication-title: Proc Natl Acad Sci doi: 10.1073/pnas.76.9.4350 – volume: 97 start-page: 12266 year: 2000 ident: ref55 article-title: Intracellular antibody-caspase-mediated cell killing: An approach for application in cancer therapy publication-title: Proc Natl Acad Sci USA doi: 10.1073/pnas.97.22.12266 – volume: 193 start-page: 706 year: 2013 ident: ref48 article-title: Human defined antigenic region on the nucleoprotein of Crimean-Congo haemorrhagic fever virus identified using truncated proteins and a bioinformatics approach publication-title: J Virol Methods doi: 10.1016/j.jviromet.2013.07.055 – volume: 86 start-page: 12294 year: 2012 ident: ref57 article-title: Structure of Crimean-Congo hemorrhagic fever virus nucleoprotein: superhelical homo-oligomers and the role of caspase-3 cleavage publication-title: J Virol doi: 10.1128/JVI.01627-12 – volume: 92 start-page: 2467 year: 2011 ident: ref13 article-title: Recent advances in the molecular and cellular biology of bunyaviruses publication-title: J Gen Virol doi: 10.1099/vir.0.035105-0 – volume: 8 start-page: 50 year: 2002 ident: ref49 article-title: Genetic characterization of the M RNA segment of Crimean Congo hemorrhagic fever virus strains, China publication-title: Emerg Infect Dis doi: 10.3201/eid0801.010087 – volume: 40 start-page: 1587 year: 2002 ident: ref21 article-title: Recombinant nucleoprotein-based enzyme-linked immunosorbent assay for detection of immunoglobulin G antibodies to Crimean-Congo hemorrhagic fever virus publication-title: J Clin Microbiol doi: 10.1128/JCM.40.5.1587-1591.2002 – volume: 81 start-page: 9 year: 2009 ident: ref28 article-title: Minimal motif mapping of a known epitope on human zona pellucida protein-4 using a peptide biosynthesis strategy publication-title: J Reprod Immunol doi: 10.1016/j.jri.2009.04.004 – reference: 2991600 - J Virol. 1985 Sep;55(3):836-9 – reference: 23948410 - Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2013 Aug;29(8):838-41 – reference: 18948048 - Int J Infect Dis. 2009 May;13(3):e105-7 – reference: 364941 - Adv Enzymol Relat Areas Mol Biol. 1978;47:45-148 – reference: 19699594 - Vet Microbiol. 2010 Jan 6;140(1-2):25-33 – reference: 5432063 - Nature. 1970 Aug 15;227(5259):680-5 – reference: 20960283 - Virol Sin. 2010 Feb;25(1):45-51 – reference: 22907379 - Methods Mol Biol. 2012;907:667-79 – reference: 17415983 - Zhonghua Liu Xing Bing Xue Za Zhi. 2006 Dec;27(12):1048-52 – reference: 22860757 - Am J Reprod Immunol. 2012 Dec;68(6):465-75 – reference: 4307033 - Adv Enzymol Relat Areas Mol Biol. 1969;32:221-96 – reference: 11050246 - Proc Natl Acad Sci U S A. 2000 Oct 24;97(22):12266-71 – reference: 7108955 - J Mol Biol. 1982 May 5;157(1):105-32 – reference: 12738657 - Clin Diagn Lab Immunol. 2003 May;10(3):489-91 – reference: 12824352 - Nucleic Acids Res. 2003 Jul 1;31(13):3497-500 – reference: 15550268 - Antiviral Res. 2004 Dec;64(3):145-60 – reference: 23933073 - J Virol Methods. 2013 Nov;193(2):706-12 – reference: 20541133 - Travel Med Infect Dis. 2010 May;8(3):139-43 – reference: 15979379 - Curr Opin Chem Biol. 2005 Aug;9(4):359-65 – reference: 16554245 - Lancet Infect Dis. 2006 Apr;6(4):203-14 – reference: 2723442 - J Immunol Methods. 1989 May 12;119(2):241-5 – reference: 19539378 - J Reprod Immunol. 2009 Jul;81(1):9-16 – reference: 11980926 - J Clin Microbiol. 2002 May;40(5):1587-91 – reference: 3101525 - Am J Trop Med Hyg. 1987 Jan;36(1):120-32 – reference: 11952140 - Viral Immunol. 2002;15(1):177-92 – reference: 2201029 - Proc Natl Acad Sci U S A. 1990 Aug;87(16):6378-82 – reference: 22951837 - J Virol. 2012 Nov;86(22):12294-303 – reference: 22001274 - J Virol Methods. 2012 Jan;179(1):70-6 – reference: 8991940 - J Med Virol. 1996 Jun;49(2):83-6 – reference: 15602720 - J Med Virol. 2005 Feb;75(2):295-9 – reference: 19553586 - J Clin Microbiol. 2009 Aug;47(8):2536-43 – reference: 113533 - J Med Entomol. 1979 May 22;15(4):307-417 – reference: 18059626 - Biotechnol Genet Eng Rev. 2007;24:1-30 – reference: 2454713 - Comput Appl Biosci. 1988 Mar;4(1):181-6 – reference: 15047845 - J Virol. 2004 Apr;78(8):4323-9 – reference: 22303367 - Front Genet. 2011 Nov 02;2:72 – reference: 21865443 - J Gen Virol. 2011 Nov;92(Pt 11):2467-84 – reference: 21123175 - J Biol Chem. 2011 Feb 4;286(5):3227-34 – reference: 1549606 - Proc Natl Acad Sci U S A. 1992 Mar 15;89(6):2429-33 – reference: 16912331 - J Virol. 2006 Sep;80(17):8834-42 – reference: 22155577 - J Virol Methods. 2012 Feb;179(2):335-41 – reference: 19864393 - J Virol. 2010 Jan;84(1):216-26 – reference: 22421137 - Proc Natl Acad Sci U S A. 2012 Mar 27;109(13):5046-51 – reference: 388439 - Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4 – reference: 11749748 - Emerg Infect Dis. 2002 Jan;8(1):50-3 – reference: 16087395 - J Clin Virol. 2006 Feb;35(2):154-9 – reference: 22875964 - J Virol. 2012 Oct;86(20):10914-23 – reference: 4600264 - Proc Natl Acad Sci U S A. 1974 May;71(5):1743-7 – reference: 7595404 - J Med Virol. 1995 Aug;46(4):293-303 – reference: 22162720 - Clin Dev Immunol. 2012;2012:831010 – reference: 23908296 - Genome Announc. 2013 Aug 01;1(4):null – reference: 18071953 - Handb Exp Pharmacol. 2008;(181):343-73 – reference: 2179464 - J Gen Virol. 1990 Mar;71 ( Pt 3):501-22 – reference: 9874664 - Clin Diagn Lab Immunol. 1999 Jan;6(1):55-60 |
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| Snippet | Crimean-Congo hemorrhagic fever (CCHF), a severe viral disease known to have occurred in over 30 countries and distinct regions, is caused by the tick-borne... |
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| SubjectTerms | Amino Acid Sequence Animal models Animals Antibodies - blood Antibodies - immunology Antigens Biology and Life Sciences Cancer Conservation Conserved Sequence Crimean hemorrhagic fever Diagnostic systems Epitope Mapping Epitopes, B-Lymphocyte - chemistry Epitopes, B-Lymphocyte - immunology Fever Gene Expression Genetic engineering Genomes Hemorrhage Hemorrhagic Fever Virus, Crimean-Congo - genetics Hemorrhagic Fever Virus, Crimean-Congo - immunology Hemorrhagic Fever, Crimean - immunology Humans Immunodominant Epitopes - chemistry Immunodominant Epitopes - genetics Immunodominant Epitopes - immunology Immunoglobulins Laboratories Life sciences Lymphocytes B Mapping Models, Molecular Molecular Sequence Data Monoclonal antibodies Nosocomial infections Nucleocapsids Nucleoproteins - chemistry Nucleoproteins - genetics Nucleoproteins - immunology Nucleotide sequence Peptide Fragments - chemistry Peptide Fragments - genetics Peptide Fragments - immunology Peptides Protein Conformation Proteins Reagents Recombinant Fusion Proteins - chemistry Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - immunology S gene Sequence Alignment Sheep Structural models Studies Three dimensional models Viral diseases Virology Viruses |
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| Title | Fine Epitope Mapping of the Central Immunodominant Region of Nucleoprotein from Crimean-Congo Hemorrhagic Fever Virus (CCHFV) |
| URI | https://www.ncbi.nlm.nih.gov/pubmed/25365026 https://www.proquest.com/docview/1619502529 https://www.proquest.com/docview/1620587375 https://pubmed.ncbi.nlm.nih.gov/PMC4217714 https://doaj.org/article/51e633136c8e4c8f92ad4d28b0c44472 http://dx.doi.org/10.1371/journal.pone.0108419 |
| Volume | 9 |
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