Zinc transporters protein level in postmortem brain of depressed subjects and suicide victims

Major depressive disorder (MDD) is a serious psychiatric illness, associated with an increasing rate of suicide. The pathogenesis of depression may be associated with the disruption of zinc (Zn) homeostasis. In the brain, several proteins that regulate Zn homeostasis are present, including Zn transp...

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Vydáno v:Journal of psychiatric research Ročník 83; s. 220 - 229
Hlavní autoři: Rafalo-Ulinska, Anna, Piotrowska, Joanna, Kryczyk, Agata, Opoka, Włodzimierz, Sowa-Kucma, Magdalena, Misztak, Paulina, Rajkowska, Grazyna, Stockmeier, Craig A., Datka, Wojciech, Nowak, Gabriel, Szewczyk, Bernadeta
Médium: Journal Article
Jazyk:angličtina
Vydáno: England Elsevier Ltd 01.12.2016
Témata:
5-HT1A
Adolescent
Adult
Autopsy
Carrier Proteins - metabolism
Cohort Studies
Depressive Disorder, Major - metabolism
Depressive Disorder, Major - pathology
Depressive Disorder, Major - psychology
Disks Large Homolog 4 Protein
Electrochemical Techniques
Female
Gene Expression Regulation - physiology
Glutamate receptors
Humans
Intracellular Signaling Peptides and Proteins - metabolism
Major depressive disorder
Male
Membrane Proteins - metabolism
Middle Aged
Prefrontal Cortex - metabolism
Psychiatry
Receptor, Serotonin, 5-HT1A - metabolism
Receptors, AMPA - metabolism
Receptors, N-Methyl-D-Aspartate - metabolism
Suicide
Suicide - psychology
Young Adult
Zinc
Zn transporters
Glutamate receptors
5-HT1A
Zn transporters
Suicide
Zinc
Major depressive disorder
Postmortem brain tissue
ISSN:0022-3956, 1879-1379, 1879-1379
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Shrnutí:Major depressive disorder (MDD) is a serious psychiatric illness, associated with an increasing rate of suicide. The pathogenesis of depression may be associated with the disruption of zinc (Zn) homeostasis. In the brain, several proteins that regulate Zn homeostasis are present, including Zn transporters (ZnTs) which remove Zn from the cytosol. The present study was designed to investigate whether depression and suicide are associated with alterations in the expression of the ZnTs protein. Protein levels of ZnT1, ZnT3, ZnT4, ZnT5 and ZnT6 were measured in postmortem brain tissue from two different cohorts. Cohort A contained 10 subjects diagnosed with MDD (7 were suicide victims) and 10 psychiatrically-normal control subjects and cohort B contained 11 non-diagnosed suicide victims and 8 sudden-death control subjects. Moreover, in cohort A we measured protein level of NMDA (GluN2A subunit), AMPA (GluA1 subunit) and 5-HT1A receptors and PSD-95. Proteins were measured in the prefrontal cortex (PFC) using Western blotting. In addition, Zn concentration was measured using a voltammetric method. There was a significant increase in protein levels of ZnT1, ZnT4, ZnT5 in the PFC in MDD, relative to control subjects, while ZnT3 protein level was decreased in MDD. There was no significant difference in the Zn concentration in the PFC between control and MDD subjects. Similarly, in the PFC of suicide victims (non-diagnosed), an increase in protein levels of ZnT1, ZnT4, ZnT5 and ZnT6 was observed. Conversely, protein levels of ZnT3 were decreased in both suicide victims and subjects with MDD, in comparison with control subjects. There was also a significant decrease in the protein level of GluA1, GluN2A, PSD-95 and 5-HT1A in MDD. Our studies suggest that alterations in Zn transport proteins are associated with the pathophysiology of MDD and suicide. •MDD and suicide in associated with alterations of ZnTs protein level in the PFC.•Increase in ZnT1, ZnT4 and ZnT5 and decrease in ZnT3 protein was found in MDD.•Suicide is associated with increase in ZnT1, 4, 5, 6 and decrease in ZnT3 protein.•Total Zn concentration is not changed in PFC of MDD.
Bibliografie:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0022-3956
1879-1379
1879-1379
DOI:10.1016/j.jpsychires.2016.09.008