Neuronal polarity selection by topography-induced focal adhesion control

Interaction between differentiating neurons and the extracellular environment guides the establishment of cell polarity during nervous system development. Developing neurons read the physical properties of the local substrate in a contact-dependent manner and retrieve essential guidance cues. In pre...

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Vydané v:	Biomaterials Ročník 31; číslo 17; s. 4682 - 4694
Hlavní autori:	Ferrari, Aldo, Cecchini, Marco, Serresi, Michela, Faraci, Paolo, Pisignano, Dario, Beltram, Fabio
Médium:	Journal Article
Jazyk:	English
Vydavateľské údaje:	Netherlands Elsevier Ltd 01.06.2010
Predmet:	Actins - metabolism Advanced Basic Science Animals Cell contractility Cell Differentiation - physiology Cell polarity Cell Polarity - physiology Contact guidance Dentistry Focal adhesions Focal Adhesions - metabolism Focal Adhesions - physiology Microscopy, Confocal Neuron Neurons - cytology PC12 Cells Rats rho-Associated Kinases - metabolism Topography Cell polarity Focal adhesions Neuron Topography Cell contractility Contact guidance
ISSN:	0142-9612, 1878-5905, 1878-5905
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Abstract	Interaction between differentiating neurons and the extracellular environment guides the establishment of cell polarity during nervous system development. Developing neurons read the physical properties of the local substrate in a contact-dependent manner and retrieve essential guidance cues. In previous works we demonstrated that PC12 cell interaction with nanogratings (alternating lines of ridges and grooves of submicron size) promotes bipolarity and alignment to the substrate topography. Here, we investigate the role of focal adhesions, cell contractility, and actin dynamics in this process. Exploiting nanoimprint lithography techniques and a cyclic olefin copolymer, we engineered biocompatible nanostructured substrates designed for high-resolution live-cell microscopy. Our results reveal that neuronal polarization and contact guidance are based on a geometrical constraint of focal adhesions resulting in an angular modulation of their maturation and persistence. We report on ROCK1/2-myosin-II pathway activity and demonstrate that ROCK-mediated contractility contributes to polarity selection during neuronal differentiation. Importantly, the selection process confined the generation of actin-supported membrane protrusions and the initiation of new neurites at the poles. Maintenance of the established polarity was independent from NGF stimulation. Altogether our results imply that focal adhesions and cell contractility stably link the topographical configuration of the extracellular environment to a corresponding neuronal polarity state.
AbstractList	Interaction between differentiating neurons and the extracellular environment guides the establishment of cell polarity during nervous system development. Developing neurons read the physical properties of the local substrate in a contact-dependent manner and retrieve essential guidance cues. In previous works we demonstrated that PC12 cell interaction with nanogratings (alternating lines of ridges and grooves of submicron size) promotes bipolarity and alignment to the substrate topography. Here, we investigate the role of focal adhesions, cell contractility, and actin dynamics in this process. Exploiting nanoimprint lithography techniques and a cyclic olefin copolymer, we engineered biocompatible nanostructured substrates designed for high-resolution live-cell microscopy. Our results reveal that neuronal polarization and contact guidance are based on a geometrical constraint of focal adhesions resulting in an angular modulation of their maturation and persistence. We report on ROCK1/2-myosin-II pathway activity and demonstrate that ROCK-mediated contractility contributes to polarity selection during neuronal differentiation. Importantly, the selection process confined the generation of actin-supported membrane protrusions and the initiation of new neurites at the poles. Maintenance of the established polarity was independent from NGF stimulation. Altogether our results imply that focal adhesions and cell contractility stably link the topographical configuration of the extracellular environment to a corresponding neuronal polarity state. Interaction between differentiating neurons and the extracellular environment guides the establishment of cell polarity during nervous system development. Developing neurons read the physical properties of the local substrate in a contact-dependent manner and retrieve essential guidance cues. In previous works we demonstrated that PC12 cell interaction with nanogratings (alternating lines of ridges and grooves of submicron size) promotes bipolarity and alignment to the substrate topography. Here, we investigate the role of focal adhesions, cell contractility, and actin dynamics in this process. Exploiting nanoimprint lithography techniques and a cyclic olefin copolymer, we engineered biocompatible nanostructured substrates designed for high-resolution live-cell microscopy. Our results reveal that neuronal polarization and contact guidance are based on a geometrical constraint of focal adhesions resulting in an angular modulation of their maturation and persistence. We report on ROCK1/2-myosin-II pathway activity and demonstrate that ROCK-mediated contractility contributes to polarity selection during neuronal differentiation. Importantly, the selection process confined the generation of actin-supported membrane protrusions and the initiation of new neurites at the poles. Maintenance of the established polarity was independent from NGF stimulation. Altogether our results imply that focal adhesions and cell contractility stably link the topographical configuration of the extracellular environment to a corresponding neuronal polarity state.Interaction between differentiating neurons and the extracellular environment guides the establishment of cell polarity during nervous system development. Developing neurons read the physical properties of the local substrate in a contact-dependent manner and retrieve essential guidance cues. In previous works we demonstrated that PC12 cell interaction with nanogratings (alternating lines of ridges and grooves of submicron size) promotes bipolarity and alignment to the substrate topography. Here, we investigate the role of focal adhesions, cell contractility, and actin dynamics in this process. Exploiting nanoimprint lithography techniques and a cyclic olefin copolymer, we engineered biocompatible nanostructured substrates designed for high-resolution live-cell microscopy. Our results reveal that neuronal polarization and contact guidance are based on a geometrical constraint of focal adhesions resulting in an angular modulation of their maturation and persistence. We report on ROCK1/2-myosin-II pathway activity and demonstrate that ROCK-mediated contractility contributes to polarity selection during neuronal differentiation. Importantly, the selection process confined the generation of actin-supported membrane protrusions and the initiation of new neurites at the poles. Maintenance of the established polarity was independent from NGF stimulation. Altogether our results imply that focal adhesions and cell contractility stably link the topographical configuration of the extracellular environment to a corresponding neuronal polarity state. Abstract Interaction between differentiating neurons and the extracellular environment guides the establishment of cell polarity during nervous system development. Developing neurons read the physical properties of the local substrate in a contact-dependent manner and retrieve essential guidance cues. In previous works we demonstrated that PC12 cell interaction with nanogratings (alternating lines of ridges and grooves of submicron size) promotes bipolarity and alignment to the substrate topography. Here, we investigate the role of focal adhesions, cell contractility, and actin dynamics in this process. Exploiting nanoimprint lithography techniques and a cyclic olefin copolymer, we engineered biocompatible nanostructured substrates designed for high-resolution live-cell microscopy. Our results reveal that neuronal polarization and contact guidance are based on a geometrical constraint of focal adhesions resulting in an angular modulation of their maturation and persistence. We report on ROCK1/2-myosin-II pathway activity and demonstrate that ROCK-mediated contractility contributes to polarity selection during neuronal differentiation. Importantly, the selection process confined the generation of actin-supported membrane protrusions and the initiation of new neurites at the poles. Maintenance of the established polarity was independent from NGF stimulation. Altogether our results imply that focal adhesions and cell contractility stably link the topographical configuration of the extracellular environment to a corresponding neuronal polarity state.
Author	Pisignano, Dario Ferrari, Aldo Beltram, Fabio Faraci, Paolo Cecchini, Marco Serresi, Michela
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BackLink	https://www.ncbi.nlm.nih.gov/pubmed/20304485$$D View this record in MEDLINE/PubMed
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Cites_doi	10.1074/jbc.270.10.5039 10.1021/nl801483w 10.1039/B803595D 10.1088/1478-3975/4/2/004 10.1016/j.medengphy.2005.04.005 10.1016/j.biomaterials.2006.07.043 10.1126/science.3055291 10.1126/science.285.5430.1028 10.1083/jcb.101.3.697 10.1016/j.biomaterials.2007.08.034 10.1523/JNEUROSCI.4209-05.2006 10.1074/jbc.271.38.23022 10.1038/340159a0 10.1529/biophysj.106.089730 10.1016/j.ejcb.2005.11.001 10.1083/jcb.200201105 10.1126/science.274.5290.1123 10.1016/j.biomaterials.2004.03.015 10.1038/nrn2236 10.1073/pnas.0500254102 10.1016/j.ydbio.2005.12.048 10.1042/bst0320416 10.1109/TBME.2009.2027424 10.1242/jcs.018648 10.1038/nrm2593 10.1088/1742-6596/100/1/012003 10.1016/j.biomaterials.2009.12.010 10.1039/b804643n 10.1016/j.biomaterials.2005.07.047 10.1088/0957-4484/18/50/505103 10.1038/35074532 10.1038/nn1762 10.1038/nrn2056 10.1111/j.1600-0854.2007.00636.x 10.1083/jcb.200307081 10.1242/dev.02854 10.1091/mbc.12.10.3103 10.1371/journal.pone.0003398 10.1126/science.1071552 10.1242/jcs.00993 10.1016/j.biomaterials.2004.09.048 10.1242/jcs.110.23.2905 10.1038/35099066 10.1242/dev.01830 10.1038/ncb1654
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Keywords	Cell polarity Focal adhesions Neuron Topography Cell contractility Contact guidance
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References	Geiger, Spatz, Bershadsky (bib40) 2009; 10 Pak, Flynn, Bamburg (bib41) 2008; 9 Mahoney, Chen, Tan, Saltzman (bib21) 2005; 26 Wu, Ramirez, Cui, Ding, Delcroix, Valletta (bib37) 2007; 8 Vaudry, Stork, Lazarovici, Eiden (bib38) 2002; 296 Shemesh, Geiger, Bershadsky, Kozlov (bib43) 2005; 102 Zaidel-Bar, Cohen, Addadi, Geiger (bib9) 2004; 32 Rottner, Krause, Gimona, Small, Wehland (bib26) 2001; 12 Dulyaninova, Patskovsky, Bresnick (bib30) 2004; 117 Gladilin, Micoulet, Hosseini, Rohr, Spatz, Eils (bib45) 2007; 4 Lamoureux, Buxbaum, Heidemann (bib34) 1989; 340 Foley, Grunwald, Nealey, Murphy (bib17) 2005; 26 Dalby (bib14) 2005; 27 Katsumi, Milanini, Kiosses, del Pozo, Kaunas, Chien (bib44) 2002; 158 Cecchini, Bumma, Serresi, Beltram (bib16) 2007; 18 Sorensen, Alekseeva, Katechia, Robertson, Riehle, Barnett (bib23) 2007; 28 Dent, Kwiatkowski, Mebane, Philippar, Barzik, Rubinson (bib36) 2007; 9 Chilton (bib3) 2006; 292 Woo, Gomez (bib13) 2006; 26 Rajnicek, Britland, McCaig (bib24) 1997; 110 Ferrari, Faraci, Cecchini, Beltram (bib25) 2010; 31 Fujisawa, Fujita, Ishizaki, Saito, Narumiya (bib28) 1996; 271 Johansson, Carlberg, Danielsen, Montelius, Kanje (bib18) 2006; 27 Ohshima, Hirasawa, Tabata, Mutoh, Adachi, Suzuki (bib6) 2007; 134 Robles, Gomez (bib4) 2006; 9 tessier-lavigne M (bib5) 1996; 274 Charron, Tessier-Lavigne (bib2) 2005; 132 Geiger, Bershadsky, Pankov, Yamada (bib8) 2001; 2 Cecchini, Ferrari, Beltram (bib19) 2008 Ferrari, Veligodskiy, Berge, Lucas, Kroschewski (bib35) 2008; 121 Huang, Borchers, Schaller, Jacobson (bib12) 2004; 164 Arnold, Hirschfeld-Warneken, Lohmuller, Heil, Blummel, Cavalcanti-Adam (bib32) 2008; 8 Giancotti, Ruoslahti (bib7) 1999; 285 Gomez, Lu, Chen, Schmidt (bib15) 2007; 28 Raz-Ben Aroush, Zaidel-Bar, Bershadsky, Wagner (bib33) 2008; 4 Cavalcanti-Adam, Volberg, Micoulet, Kessler, Geiger, Spatz (bib31) 2007; 92 Bershadsky, Ballestrem, Carramusa, Zilberman, Gilquin, Khochbin (bib11) 2006; 85 Arimura, Kaibuchi (bib1) 2007; 8 Hanson, Motala, Heien, Gillette, Sweedler, Nuzzo (bib22) 2009; 9 Dodd, Jessell (bib39) 1988; 242 Salgia, Li, Lo, Brunkhorst, Kansas, Sobhany (bib27) 1995; 270 Joshi, Chu, Buxbaum, Heidemann (bib42) 1985; 101 Ferrari, Cecchini (bib20) 2009; 56 Shea, Wells, Garner, Jones (bib29) 2008; 3 Balaban, Schwarz, Riveline, Goichberg, Tzur, Sabanay (bib10) 2001; 3 Cecchini (10.1016/j.biomaterials.2010.02.032_bib19) 2008 Pak (10.1016/j.biomaterials.2010.02.032_bib41) 2008; 9 Foley (10.1016/j.biomaterials.2010.02.032_bib17) 2005; 26 Rajnicek (10.1016/j.biomaterials.2010.02.032_bib24) 1997; 110 Giancotti (10.1016/j.biomaterials.2010.02.032_bib7) 1999; 285 Gomez (10.1016/j.biomaterials.2010.02.032_bib15) 2007; 28 Wu (10.1016/j.biomaterials.2010.02.032_bib37) 2007; 8 Joshi (10.1016/j.biomaterials.2010.02.032_bib42) 1985; 101 Geiger (10.1016/j.biomaterials.2010.02.032_bib8) 2001; 2 Balaban (10.1016/j.biomaterials.2010.02.032_bib10) 2001; 3 Shemesh (10.1016/j.biomaterials.2010.02.032_bib43) 2005; 102 Dalby (10.1016/j.biomaterials.2010.02.032_bib14) 2005; 27 Sorensen (10.1016/j.biomaterials.2010.02.032_bib23) 2007; 28 Zaidel-Bar (10.1016/j.biomaterials.2010.02.032_bib9) 2004; 32 Dent (10.1016/j.biomaterials.2010.02.032_bib36) 2007; 9 Vaudry (10.1016/j.biomaterials.2010.02.032_bib38) 2002; 296 Gladilin (10.1016/j.biomaterials.2010.02.032_bib45) 2007; 4 Charron (10.1016/j.biomaterials.2010.02.032_bib2) 2005; 132 Ferrari (10.1016/j.biomaterials.2010.02.032_bib35) 2008; 121 Johansson (10.1016/j.biomaterials.2010.02.032_bib18) 2006; 27 Lamoureux (10.1016/j.biomaterials.2010.02.032_bib34) 1989; 340 Robles (10.1016/j.biomaterials.2010.02.032_bib4) 2006; 9 Dodd (10.1016/j.biomaterials.2010.02.032_bib39) 1988; 242 tessier-lavigne M (10.1016/j.biomaterials.2010.02.032_bib5) 1996; 274 Chilton (10.1016/j.biomaterials.2010.02.032_bib3) 2006; 292 Hanson (10.1016/j.biomaterials.2010.02.032_bib22) 2009; 9 Bershadsky (10.1016/j.biomaterials.2010.02.032_bib11) 2006; 85 Rottner (10.1016/j.biomaterials.2010.02.032_bib26) 2001; 12 Fujisawa (10.1016/j.biomaterials.2010.02.032_bib28) 1996; 271 Mahoney (10.1016/j.biomaterials.2010.02.032_bib21) 2005; 26 Ferrari (10.1016/j.biomaterials.2010.02.032_bib20) 2009; 56 Raz-Ben Aroush (10.1016/j.biomaterials.2010.02.032_bib33) 2008; 4 Arimura (10.1016/j.biomaterials.2010.02.032_bib1) 2007; 8 Ohshima (10.1016/j.biomaterials.2010.02.032_bib6) 2007; 134 Ferrari (10.1016/j.biomaterials.2010.02.032_bib25) 2010; 31 Katsumi (10.1016/j.biomaterials.2010.02.032_bib44) 2002; 158 Huang (10.1016/j.biomaterials.2010.02.032_bib12) 2004; 164 Cavalcanti-Adam (10.1016/j.biomaterials.2010.02.032_bib31) 2007; 92 Woo (10.1016/j.biomaterials.2010.02.032_bib13) 2006; 26 Geiger (10.1016/j.biomaterials.2010.02.032_bib40) 2009; 10 Salgia (10.1016/j.biomaterials.2010.02.032_bib27) 1995; 270 Shea (10.1016/j.biomaterials.2010.02.032_bib29) 2008; 3 Arnold (10.1016/j.biomaterials.2010.02.032_bib32) 2008; 8 Cecchini (10.1016/j.biomaterials.2010.02.032_bib16) 2007; 18 Dulyaninova (10.1016/j.biomaterials.2010.02.032_bib30) 2004; 117
References_xml	– start-page: 012003 year: 2008 ident: bib19 article-title: PC12 polarity on biopolymer nanogratings publication-title: J Phys Conf Ser – volume: 8 start-page: 194 year: 2007 end-page: 205 ident: bib1 article-title: Neuronal polarity: from extracellular signals to intracellular mechanisms publication-title: Nat Rev Neurosci – volume: 28 start-page: 271 year: 2007 end-page: 284 ident: bib15 article-title: Immobilized nerve growth factor and microtopography have distinct effects on polarization versus axon elongation in hippocampal cells in culture publication-title: Biomaterials – volume: 26 start-page: 1418 year: 2006 end-page: 1428 ident: bib13 article-title: Rac1 and RhoA promote neurite outgrowth through formation and stabilization of growth cone point contacts publication-title: J Neurosci – volume: 132 start-page: 2251 year: 2005 end-page: 2262 ident: bib2 article-title: Novel brain wiring functions for classical morphogens: a role as graded positional cues in axon guidance publication-title: Development – volume: 101 start-page: 697 year: 1985 end-page: 705 ident: bib42 article-title: Tension and compression in the cytoskeleton of PC 12 neurites publication-title: J Cell Biol – volume: 9 start-page: 122 year: 2009 end-page: 131 ident: bib22 article-title: Textural guidance cues for controlling process outgrowth of mammalian neurons publication-title: Lab Chip – volume: 285 start-page: 1028 year: 1999 end-page: 1032 ident: bib7 article-title: Transduction – integrin signaling publication-title: Science – volume: 271 start-page: 23,022 year: 1996 end-page: 23,028 ident: bib28 article-title: Identification of the Rhobinding domain of p160ROCK, a Rho-associated coiled-coil containing protein kinase publication-title: J Biol Chem – volume: 3 start-page: 466 year: 2001 end-page: 472 ident: bib10 article-title: Force and focal adhesion assembly: a close relationship studied using elastic micropatterned substrates publication-title: Nat Cell Biol – volume: 121 start-page: 3649 year: 2008 end-page: 3663 ident: bib35 article-title: ROCK-mediated contractility, tight junctions and channels contribute to the conversion of a preapical patch into apical surface during isochoric lumen initiation publication-title: J Cell Sci – volume: 18 start-page: 1 year: 2007 end-page: 7 ident: bib16 article-title: PC12 differentiation on biopolymer nanostructures publication-title: Nanotechnology – volume: 27 start-page: 1251 year: 2006 end-page: 1258 ident: bib18 article-title: Axonal outgrowth on nano-imprinted patterns publication-title: Biomaterials – volume: 110 start-page: 2905 year: 1997 end-page: 2913 ident: bib24 article-title: Contact guidance of CNS neurites on grooved quartz: influence of groove dimensions, neuronal age and cell type publication-title: J Cell Sci – volume: 9 start-page: 1347 year: 2007 end-page: 1359 ident: bib36 article-title: Filopodia are required for cortical neurite initiation publication-title: Nat Cell Biol – volume: 296 start-page: 1648 year: 2002 end-page: 1649 ident: bib38 article-title: Signaling pathways for PC12 cell differentiation: making the right connections publication-title: Science – volume: 8 start-page: 2063 year: 2008 end-page: 2069 ident: bib32 article-title: Induction of cell polarization and migration by a gradient of nanoscale variations in adhesive ligand spacing publication-title: Nano Lett – volume: 2 start-page: 793 year: 2001 end-page: 805 ident: bib8 article-title: Transmembrane extracellular matrix-cytoskeleton crosstalk publication-title: Nat Rev Mol Cell Biol – volume: 31 start-page: 2565 year: 2010 end-page: 2573 ident: bib25 article-title: The effect of alternative neuronal differentiation pathways on PC12 cell adhesion and neurite alignment to nanogratings publication-title: Biomaterials – volume: 164 start-page: 593 year: 2004 end-page: 602 ident: bib12 article-title: Phosphorylation of paxillin by p38MAPK is involved in the neurite extension of PC-12 cells publication-title: J Cell Biol – volume: 85 start-page: 165 year: 2006 end-page: 173 ident: bib11 article-title: Assembly and mechanosensory function of focal adhesions: experiments and models publication-title: Eur J Cell Biol – volume: 4 start-page: 104 year: 2007 end-page: 113 ident: bib45 article-title: 3D finite element analysis of uniaxial cell stretching: from image to insight publication-title: Phys Biol – volume: 3 start-page: e3398 year: 2008 ident: bib29 article-title: ROCK1 and LIMK2 interact in spread but not blebbing cancer cells publication-title: PLoS One – volume: 92 start-page: 2964 year: 2007 end-page: 2974 ident: bib31 article-title: Cell spreading and focal adhesion dynamics are regulated by spacing of integrin ligands publication-title: Biophys J – volume: 242 start-page: 692 year: 1988 end-page: 699 ident: bib39 article-title: Axon guidance and the patterning of neuronal projections in vertebrates publication-title: Science – volume: 117 start-page: 1481 year: 2004 end-page: 1493 ident: bib30 article-title: The N-terminus of the long MLCK induces a disruption in normal spindle morphology and metaphase arrest publication-title: J Cell Sci – volume: 158 start-page: 153 year: 2002 end-page: 164 ident: bib44 article-title: Effects of cell tension on the small GTPase Rac publication-title: J Cell Biol – volume: 340 start-page: 156 year: 1989 end-page: 162 ident: bib34 article-title: Direct evidence that growth cones pull publication-title: Nature – volume: 270 start-page: 5039 year: 1995 end-page: 5047 ident: bib27 article-title: Molecular cloning of human paxillin, a focal adhesion protein phosphorylated by P210BCR/ABL publication-title: J Biol Chem – volume: 134 start-page: 2273 year: 2007 end-page: 2282 ident: bib6 article-title: Cdk5 is required for multipolar-to-bipolar transition during radial neuronal migration and proper dendrite development of pyramidal neurons in the cerebral cortex publication-title: Development – volume: 274 start-page: 1123 year: 1996 end-page: 1133 ident: bib5 article-title: Goodman CS. The molecular biology of axon guidance publication-title: Science – volume: 9 start-page: 1274 year: 2006 end-page: 1283 ident: bib4 article-title: Focal adhesion kinase signaling at sites of integrin-mediated adhesion controls axon pathfinding publication-title: Nat Neurosci – volume: 32 start-page: 416 year: 2004 end-page: 420 ident: bib9 article-title: Hierarchical assembly of cell-matrix adhesion complexes publication-title: Biochem Soc Trans – volume: 292 start-page: 13 year: 2006 end-page: 24 ident: bib3 article-title: Molecular mechanisms of axon guidance publication-title: Dev Biol – volume: 56 start-page: 2692 year: 2009 end-page: 2696 ident: bib20 article-title: Degl Innocenti R, Beltram F. Directional PC12 cell migration along plastic nanotracks publication-title: IEEE Trans Biomed Eng – volume: 9 start-page: 136 year: 2008 end-page: 147 ident: bib41 article-title: Actin-binding proteins take the reins in growth cones publication-title: Nat Rev Neurosci – volume: 4 start-page: 2410 year: 2008 end-page: 2417 ident: bib33 article-title: Temporal evolution of cell focal adhesions: experimental observations and shear stress profiles publication-title: Soft Matter – volume: 28 start-page: 5498 year: 2007 end-page: 5508 ident: bib23 article-title: Longterm neurite orientation on astrocyte monolayers aligned by microtopography publication-title: Biomaterials – volume: 12 start-page: 3103 year: 2001 end-page: 3113 ident: bib26 article-title: Zyxin is not colocalized with vasodilator-stimulated phosphoprotein (VASP) at lamellipodial tips and exhibits different dynamics to vinculin, paxillin, and VASP in focal adhesions publication-title: Mol Biol Cell – volume: 26 start-page: 3639 year: 2005 end-page: 3644 ident: bib17 article-title: Cooperative modulation of neuritogenesis by PC12 cells by topography and nerve growth factor publication-title: Biomaterials – volume: 26 start-page: 771 year: 2005 end-page: 778 ident: bib21 article-title: The influence of microchannels on neurite growth and architecture publication-title: Biomaterials – volume: 10 start-page: 21 year: 2009 end-page: 33 ident: bib40 article-title: Environmental sensing through focal adhesions publication-title: Nat Rev Mol Cell Biol – volume: 102 start-page: 12383 year: 2005 end-page: 12388 ident: bib43 article-title: Focal adhesions as mechanosensors: a physical mechanism publication-title: Proc Natl Acad Sci U S A – volume: 27 start-page: 730 year: 2005 end-page: 742 ident: bib14 article-title: Topographically induced direct cell mechanotransduction publication-title: Med Eng Phys – volume: 8 start-page: 1503 year: 2007 end-page: 1520 ident: bib37 article-title: A functional dynein-microtubule network is required for NGF signaling through the Rap1/MAPK pathway publication-title: Traffic – volume: 270 start-page: 5039 year: 1995 ident: 10.1016/j.biomaterials.2010.02.032_bib27 article-title: Molecular cloning of human paxillin, a focal adhesion protein phosphorylated by P210BCR/ABL publication-title: J Biol Chem doi: 10.1074/jbc.270.10.5039 – volume: 8 start-page: 2063 year: 2008 ident: 10.1016/j.biomaterials.2010.02.032_bib32 article-title: Induction of cell polarization and migration by a gradient of nanoscale variations in adhesive ligand spacing publication-title: Nano Lett doi: 10.1021/nl801483w – volume: 9 start-page: 122 year: 2009 ident: 10.1016/j.biomaterials.2010.02.032_bib22 article-title: Textural guidance cues for controlling process outgrowth of mammalian neurons publication-title: Lab Chip doi: 10.1039/B803595D – volume: 4 start-page: 104 year: 2007 ident: 10.1016/j.biomaterials.2010.02.032_bib45 article-title: 3D finite element analysis of uniaxial cell stretching: from image to insight publication-title: Phys Biol doi: 10.1088/1478-3975/4/2/004 – volume: 27 start-page: 730 year: 2005 ident: 10.1016/j.biomaterials.2010.02.032_bib14 article-title: Topographically induced direct cell mechanotransduction publication-title: Med Eng Phys doi: 10.1016/j.medengphy.2005.04.005 – volume: 28 start-page: 271 year: 2007 ident: 10.1016/j.biomaterials.2010.02.032_bib15 article-title: Immobilized nerve growth factor and microtopography have distinct effects on polarization versus axon elongation in hippocampal cells in culture publication-title: Biomaterials doi: 10.1016/j.biomaterials.2006.07.043 – volume: 242 start-page: 692 year: 1988 ident: 10.1016/j.biomaterials.2010.02.032_bib39 article-title: Axon guidance and the patterning of neuronal projections in vertebrates publication-title: Science doi: 10.1126/science.3055291 – volume: 285 start-page: 1028 year: 1999 ident: 10.1016/j.biomaterials.2010.02.032_bib7 article-title: Transduction – integrin signaling publication-title: Science doi: 10.1126/science.285.5430.1028 – volume: 101 start-page: 697 year: 1985 ident: 10.1016/j.biomaterials.2010.02.032_bib42 article-title: Tension and compression in the cytoskeleton of PC 12 neurites publication-title: J Cell Biol doi: 10.1083/jcb.101.3.697 – volume: 28 start-page: 5498 year: 2007 ident: 10.1016/j.biomaterials.2010.02.032_bib23 article-title: Longterm neurite orientation on astrocyte monolayers aligned by microtopography publication-title: Biomaterials doi: 10.1016/j.biomaterials.2007.08.034 – volume: 26 start-page: 1418 year: 2006 ident: 10.1016/j.biomaterials.2010.02.032_bib13 article-title: Rac1 and RhoA promote neurite outgrowth through formation and stabilization of growth cone point contacts publication-title: J Neurosci doi: 10.1523/JNEUROSCI.4209-05.2006 – volume: 271 start-page: 23,022 year: 1996 ident: 10.1016/j.biomaterials.2010.02.032_bib28 article-title: Identification of the Rhobinding domain of p160ROCK, a Rho-associated coiled-coil containing protein kinase publication-title: J Biol Chem doi: 10.1074/jbc.271.38.23022 – volume: 340 start-page: 156 year: 1989 ident: 10.1016/j.biomaterials.2010.02.032_bib34 article-title: Direct evidence that growth cones pull publication-title: Nature doi: 10.1038/340159a0 – volume: 92 start-page: 2964 year: 2007 ident: 10.1016/j.biomaterials.2010.02.032_bib31 article-title: Cell spreading and focal adhesion dynamics are regulated by spacing of integrin ligands publication-title: Biophys J doi: 10.1529/biophysj.106.089730 – volume: 85 start-page: 165 year: 2006 ident: 10.1016/j.biomaterials.2010.02.032_bib11 article-title: Assembly and mechanosensory function of focal adhesions: experiments and models publication-title: Eur J Cell Biol doi: 10.1016/j.ejcb.2005.11.001 – volume: 158 start-page: 153 year: 2002 ident: 10.1016/j.biomaterials.2010.02.032_bib44 article-title: Effects of cell tension on the small GTPase Rac publication-title: J Cell Biol doi: 10.1083/jcb.200201105 – volume: 274 start-page: 1123 year: 1996 ident: 10.1016/j.biomaterials.2010.02.032_bib5 article-title: Goodman CS. The molecular biology of axon guidance publication-title: Science doi: 10.1126/science.274.5290.1123 – volume: 26 start-page: 771 year: 2005 ident: 10.1016/j.biomaterials.2010.02.032_bib21 article-title: The influence of microchannels on neurite growth and architecture publication-title: Biomaterials doi: 10.1016/j.biomaterials.2004.03.015 – volume: 9 start-page: 136 year: 2008 ident: 10.1016/j.biomaterials.2010.02.032_bib41 article-title: Actin-binding proteins take the reins in growth cones publication-title: Nat Rev Neurosci doi: 10.1038/nrn2236 – volume: 102 start-page: 12383 year: 2005 ident: 10.1016/j.biomaterials.2010.02.032_bib43 article-title: Focal adhesions as mechanosensors: a physical mechanism publication-title: Proc Natl Acad Sci U S A doi: 10.1073/pnas.0500254102 – volume: 292 start-page: 13 year: 2006 ident: 10.1016/j.biomaterials.2010.02.032_bib3 article-title: Molecular mechanisms of axon guidance publication-title: Dev Biol doi: 10.1016/j.ydbio.2005.12.048 – volume: 32 start-page: 416 year: 2004 ident: 10.1016/j.biomaterials.2010.02.032_bib9 article-title: Hierarchical assembly of cell-matrix adhesion complexes publication-title: Biochem Soc Trans doi: 10.1042/bst0320416 – volume: 56 start-page: 2692 year: 2009 ident: 10.1016/j.biomaterials.2010.02.032_bib20 article-title: Degl Innocenti R, Beltram F. Directional PC12 cell migration along plastic nanotracks publication-title: IEEE Trans Biomed Eng doi: 10.1109/TBME.2009.2027424 – volume: 121 start-page: 3649 year: 2008 ident: 10.1016/j.biomaterials.2010.02.032_bib35 article-title: ROCK-mediated contractility, tight junctions and channels contribute to the conversion of a preapical patch into apical surface during isochoric lumen initiation publication-title: J Cell Sci doi: 10.1242/jcs.018648 – volume: 10 start-page: 21 year: 2009 ident: 10.1016/j.biomaterials.2010.02.032_bib40 article-title: Environmental sensing through focal adhesions publication-title: Nat Rev Mol Cell Biol doi: 10.1038/nrm2593 – start-page: 012003 year: 2008 ident: 10.1016/j.biomaterials.2010.02.032_bib19 article-title: PC12 polarity on biopolymer nanogratings publication-title: J Phys Conf Ser doi: 10.1088/1742-6596/100/1/012003 – volume: 31 start-page: 2565 year: 2010 ident: 10.1016/j.biomaterials.2010.02.032_bib25 article-title: The effect of alternative neuronal differentiation pathways on PC12 cell adhesion and neurite alignment to nanogratings publication-title: Biomaterials doi: 10.1016/j.biomaterials.2009.12.010 – volume: 4 start-page: 2410 year: 2008 ident: 10.1016/j.biomaterials.2010.02.032_bib33 article-title: Temporal evolution of cell focal adhesions: experimental observations and shear stress profiles publication-title: Soft Matter doi: 10.1039/b804643n – volume: 27 start-page: 1251 year: 2006 ident: 10.1016/j.biomaterials.2010.02.032_bib18 article-title: Axonal outgrowth on nano-imprinted patterns publication-title: Biomaterials doi: 10.1016/j.biomaterials.2005.07.047 – volume: 18 start-page: 1 year: 2007 ident: 10.1016/j.biomaterials.2010.02.032_bib16 article-title: PC12 differentiation on biopolymer nanostructures publication-title: Nanotechnology doi: 10.1088/0957-4484/18/50/505103 – volume: 3 start-page: 466 year: 2001 ident: 10.1016/j.biomaterials.2010.02.032_bib10 article-title: Force and focal adhesion assembly: a close relationship studied using elastic micropatterned substrates publication-title: Nat Cell Biol doi: 10.1038/35074532 – volume: 9 start-page: 1274 year: 2006 ident: 10.1016/j.biomaterials.2010.02.032_bib4 article-title: Focal adhesion kinase signaling at sites of integrin-mediated adhesion controls axon pathfinding publication-title: Nat Neurosci doi: 10.1038/nn1762 – volume: 8 start-page: 194 year: 2007 ident: 10.1016/j.biomaterials.2010.02.032_bib1 article-title: Neuronal polarity: from extracellular signals to intracellular mechanisms publication-title: Nat Rev Neurosci doi: 10.1038/nrn2056 – volume: 8 start-page: 1503 year: 2007 ident: 10.1016/j.biomaterials.2010.02.032_bib37 article-title: A functional dynein-microtubule network is required for NGF signaling through the Rap1/MAPK pathway publication-title: Traffic doi: 10.1111/j.1600-0854.2007.00636.x – volume: 164 start-page: 593 year: 2004 ident: 10.1016/j.biomaterials.2010.02.032_bib12 article-title: Phosphorylation of paxillin by p38MAPK is involved in the neurite extension of PC-12 cells publication-title: J Cell Biol doi: 10.1083/jcb.200307081 – volume: 134 start-page: 2273 year: 2007 ident: 10.1016/j.biomaterials.2010.02.032_bib6 article-title: Cdk5 is required for multipolar-to-bipolar transition during radial neuronal migration and proper dendrite development of pyramidal neurons in the cerebral cortex publication-title: Development doi: 10.1242/dev.02854 – volume: 12 start-page: 3103 year: 2001 ident: 10.1016/j.biomaterials.2010.02.032_bib26 article-title: Zyxin is not colocalized with vasodilator-stimulated phosphoprotein (VASP) at lamellipodial tips and exhibits different dynamics to vinculin, paxillin, and VASP in focal adhesions publication-title: Mol Biol Cell doi: 10.1091/mbc.12.10.3103 – volume: 3 start-page: e3398 year: 2008 ident: 10.1016/j.biomaterials.2010.02.032_bib29 article-title: ROCK1 and LIMK2 interact in spread but not blebbing cancer cells publication-title: PLoS One doi: 10.1371/journal.pone.0003398 – volume: 296 start-page: 1648 year: 2002 ident: 10.1016/j.biomaterials.2010.02.032_bib38 article-title: Signaling pathways for PC12 cell differentiation: making the right connections publication-title: Science doi: 10.1126/science.1071552 – volume: 117 start-page: 1481 year: 2004 ident: 10.1016/j.biomaterials.2010.02.032_bib30 article-title: The N-terminus of the long MLCK induces a disruption in normal spindle morphology and metaphase arrest publication-title: J Cell Sci doi: 10.1242/jcs.00993 – volume: 26 start-page: 3639 year: 2005 ident: 10.1016/j.biomaterials.2010.02.032_bib17 article-title: Cooperative modulation of neuritogenesis by PC12 cells by topography and nerve growth factor publication-title: Biomaterials doi: 10.1016/j.biomaterials.2004.09.048 – volume: 110 start-page: 2905 issue: Pt 23 year: 1997 ident: 10.1016/j.biomaterials.2010.02.032_bib24 article-title: Contact guidance of CNS neurites on grooved quartz: influence of groove dimensions, neuronal age and cell type publication-title: J Cell Sci doi: 10.1242/jcs.110.23.2905 – volume: 2 start-page: 793 year: 2001 ident: 10.1016/j.biomaterials.2010.02.032_bib8 article-title: Transmembrane extracellular matrix-cytoskeleton crosstalk publication-title: Nat Rev Mol Cell Biol doi: 10.1038/35099066 – volume: 132 start-page: 2251 year: 2005 ident: 10.1016/j.biomaterials.2010.02.032_bib2 article-title: Novel brain wiring functions for classical morphogens: a role as graded positional cues in axon guidance publication-title: Development doi: 10.1242/dev.01830 – volume: 9 start-page: 1347 year: 2007 ident: 10.1016/j.biomaterials.2010.02.032_bib36 article-title: Filopodia are required for cortical neurite initiation publication-title: Nat Cell Biol doi: 10.1038/ncb1654
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Snippet	Interaction between differentiating neurons and the extracellular environment guides the establishment of cell polarity during nervous system development.... Abstract Interaction between differentiating neurons and the extracellular environment guides the establishment of cell polarity during nervous system...
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SubjectTerms	Actins - metabolism Advanced Basic Science Animals Cell contractility Cell Differentiation - physiology Cell polarity Cell Polarity - physiology Contact guidance Dentistry Focal adhesions Focal Adhesions - metabolism Focal Adhesions - physiology Microscopy, Confocal Neuron Neurons - cytology PC12 Cells Rats rho-Associated Kinases - metabolism Topography
Title	Neuronal polarity selection by topography-induced focal adhesion control
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