Genome transplantation in bacteria: changing one species to another

As a step toward propagation of synthetic genomes, we completely replaced the genome of a bacterial cell with one from another species by transplanting a whole genome as naked DNA. Intact genomic DNA from Mycoplasma mycoides large colony (LC), virtually free of protein, was transplanted into Mycopla...

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Veröffentlicht in:Science (American Association for the Advancement of Science) Jg. 317; H. 5838; S. 632
Hauptverfasser: Lartigue, Carole, Glass, John I, Alperovich, Nina, Pieper, Rembert, Parmar, Prashanth P, Hutchison, 3rd, Clyde A, Smith, Hamilton O, Venter, J Craig
Format: Journal Article
Sprache:Englisch
Veröffentlicht: United States 03.08.2007
Schlagworte:
Acetate Kinase - chemistry
Acetate Kinase - genetics
Amino Acid Sequence
DNA, Bacterial - genetics
DNA, Bacterial - isolation & purification
Genome, Bacterial
Genotype
Molecular Sequence Data
Mycoplasma - chemistry
Mycoplasma - genetics
Mycoplasma mycoides - chemistry
Mycoplasma mycoides - genetics
Phenotype
Polyethylene Glycols
Proteome - analysis
Recombination, Genetic
Sequence Analysis, DNA
Transformation, Bacterial
ISSN:1095-9203, 1095-9203
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Beschreibung
Zusammenfassung:As a step toward propagation of synthetic genomes, we completely replaced the genome of a bacterial cell with one from another species by transplanting a whole genome as naked DNA. Intact genomic DNA from Mycoplasma mycoides large colony (LC), virtually free of protein, was transplanted into Mycoplasma capricolum cells by polyethylene glycol-mediated transformation. Cells selected for tetracycline resistance, carried by the M. mycoides LC chromosome, contain the complete donor genome and are free of detectable recipient genomic sequences. These cells that result from genome transplantation are phenotypically identical to the M. mycoides LC donor strain as judged by several criteria.
Bibliographie:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1095-9203
1095-9203
DOI:10.1126/science.1144622