Genome transplantation in bacteria: changing one species to another

As a step toward propagation of synthetic genomes, we completely replaced the genome of a bacterial cell with one from another species by transplanting a whole genome as naked DNA. Intact genomic DNA from Mycoplasma mycoides large colony (LC), virtually free of protein, was transplanted into Mycopla...

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Vydáno v:Science (American Association for the Advancement of Science) Ročník 317; číslo 5838; s. 632
Hlavní autoři: Lartigue, Carole, Glass, John I, Alperovich, Nina, Pieper, Rembert, Parmar, Prashanth P, Hutchison, 3rd, Clyde A, Smith, Hamilton O, Venter, J Craig
Médium: Journal Article
Jazyk:angličtina
Vydáno: United States 03.08.2007
Témata:
Acetate Kinase - chemistry
Acetate Kinase - genetics
Amino Acid Sequence
DNA, Bacterial - genetics
DNA, Bacterial - isolation & purification
Genome, Bacterial
Genotype
Molecular Sequence Data
Mycoplasma - chemistry
Mycoplasma - genetics
Mycoplasma mycoides - chemistry
Mycoplasma mycoides - genetics
Phenotype
Polyethylene Glycols
Proteome - analysis
Recombination, Genetic
Sequence Analysis, DNA
Transformation, Bacterial
ISSN:1095-9203, 1095-9203
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Abstract As a step toward propagation of synthetic genomes, we completely replaced the genome of a bacterial cell with one from another species by transplanting a whole genome as naked DNA. Intact genomic DNA from Mycoplasma mycoides large colony (LC), virtually free of protein, was transplanted into Mycoplasma capricolum cells by polyethylene glycol-mediated transformation. Cells selected for tetracycline resistance, carried by the M. mycoides LC chromosome, contain the complete donor genome and are free of detectable recipient genomic sequences. These cells that result from genome transplantation are phenotypically identical to the M. mycoides LC donor strain as judged by several criteria.
AbstractList As a step toward propagation of synthetic genomes, we completely replaced the genome of a bacterial cell with one from another species by transplanting a whole genome as naked DNA. Intact genomic DNA from Mycoplasma mycoides large colony (LC), virtually free of protein, was transplanted into Mycoplasma capricolum cells by polyethylene glycol-mediated transformation. Cells selected for tetracycline resistance, carried by the M. mycoides LC chromosome, contain the complete donor genome and are free of detectable recipient genomic sequences. These cells that result from genome transplantation are phenotypically identical to the M. mycoides LC donor strain as judged by several criteria.
As a step toward propagation of synthetic genomes, we completely replaced the genome of a bacterial cell with one from another species by transplanting a whole genome as naked DNA. Intact genomic DNA from Mycoplasma mycoides large colony (LC), virtually free of protein, was transplanted into Mycoplasma capricolum cells by polyethylene glycol-mediated transformation. Cells selected for tetracycline resistance, carried by the M. mycoides LC chromosome, contain the complete donor genome and are free of detectable recipient genomic sequences. These cells that result from genome transplantation are phenotypically identical to the M. mycoides LC donor strain as judged by several criteria.As a step toward propagation of synthetic genomes, we completely replaced the genome of a bacterial cell with one from another species by transplanting a whole genome as naked DNA. Intact genomic DNA from Mycoplasma mycoides large colony (LC), virtually free of protein, was transplanted into Mycoplasma capricolum cells by polyethylene glycol-mediated transformation. Cells selected for tetracycline resistance, carried by the M. mycoides LC chromosome, contain the complete donor genome and are free of detectable recipient genomic sequences. These cells that result from genome transplantation are phenotypically identical to the M. mycoides LC donor strain as judged by several criteria.
Author Hutchison, 3rd, Clyde A
Venter, J Craig
Alperovich, Nina
Pieper, Rembert
Lartigue, Carole
Glass, John I
Parmar, Prashanth P
Smith, Hamilton O
Author_xml – sequence: 1
  givenname: Carole
  surname: Lartigue
  fullname: Lartigue, Carole
  organization: J. Craig Venter Institute, Rockville, MD 20850, USA
– sequence: 2
  givenname: John I
  surname: Glass
  fullname: Glass, John I
– sequence: 3
  givenname: Nina
  surname: Alperovich
  fullname: Alperovich, Nina
– sequence: 4
  givenname: Rembert
  surname: Pieper
  fullname: Pieper, Rembert
– sequence: 5
  givenname: Prashanth P
  surname: Parmar
  fullname: Parmar, Prashanth P
– sequence: 6
  givenname: Clyde A
  surname: Hutchison, 3rd
  fullname: Hutchison, 3rd, Clyde A
– sequence: 7
  givenname: Hamilton O
  surname: Smith
  fullname: Smith, Hamilton O
– sequence: 8
  givenname: J Craig
  surname: Venter
  fullname: Venter, J Craig
BackLink https://www.ncbi.nlm.nih.gov/pubmed/17600181$$D View this record in MEDLINE/PubMed
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PublicationTitle Science (American Association for the Advancement of Science)
PublicationTitleAlternate Science
PublicationYear 2007
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Snippet As a step toward propagation of synthetic genomes, we completely replaced the genome of a bacterial cell with one from another species by transplanting a whole...
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StartPage 632
SubjectTerms Acetate Kinase - chemistry
Acetate Kinase - genetics
Amino Acid Sequence
DNA, Bacterial - genetics
DNA, Bacterial - isolation & purification
Genome, Bacterial
Genotype
Molecular Sequence Data
Mycoplasma - chemistry
Mycoplasma - genetics
Mycoplasma mycoides - chemistry
Mycoplasma mycoides - genetics
Phenotype
Polyethylene Glycols
Proteome - analysis
Recombination, Genetic
Sequence Analysis, DNA
Transformation, Bacterial
Title Genome transplantation in bacteria: changing one species to another
URI https://www.ncbi.nlm.nih.gov/pubmed/17600181
https://www.proquest.com/docview/68125309
Volume 317
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