Evaluation of two molecular methods for the detection of Yellow fever virus genome

Yellow fever virus (YFV), a member of the family Flaviviridae, genus Flavivirus is endemic to tropical areas of Africa and South America and is among the arboviruses that pose a threat to public health. Recent outbreaks in Brazil, Bolivia, and Paraguay and the observation that vectors capable of tra...

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Bibliographic Details
Published in:	Journal of virological methods Vol. 174; no. 1-2; pp. 29 - 34
Main Authors:	Nunes, Marcio R.T., Palacios, Gustavo, Nunes, Keley N.B., Casseb, Samir M.M., Martins, Lívia C., Quaresma, Juarez A.S., Savji, Nazir, Lipkin, W. Ian, Vasconcelos, Pedro F.C.
Format:	Journal Article
Language:	English
Published:	Kidlington Elsevier B.V 01.06.2011 Elsevier
Subjects:	acute course Africa Animals arboviruses Biological and medical sciences blood serum Bolivia Brazil Cricetinae diagnostic techniques Flaviviridae Flavivirus Fundamental and applied biological sciences. Psychology genome Genomes Humans Mesocricetus Mesocricetus auratus Microbiology molecular diagnostic Molecular Diagnostic Techniques - methods monitoring Paraguay Public health quantitative polymerase chain reaction reverse transcriptase polymerase chain reaction Reverse Transcriptase Polymerase Chain Reaction - methods RT-hemi-nested-PCR Serum - virology South America SYBR® Green qRT-PCR Techniques used in virology urban areas Virology Virology - methods Yellow Fever - diagnosis Yellow Fever - virology Yellow fever virus Yellow fever virus - genetics Yellow fever virus - isolation & purification Brazil Bolivia Paraguay Africa South America molecular diagnostic RT-hemi-nested-PCR SYBR® Green qRT-PCR Yellow fever virus Nested polymerase chain reaction Method Flavivirus Virus Polymerase chain reaction SYBR Yellow fever virus molecular diagnostic Flaviviridae Detection Genome Yellow fever group virus Reverse transcription polymerase chain reaction Green qRT-PCR
ISSN:	0166-0934, 1879-0984, 1879-0984
Online Access:	Get full text
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Summary:	Yellow fever virus (YFV), a member of the family Flaviviridae, genus Flavivirus is endemic to tropical areas of Africa and South America and is among the arboviruses that pose a threat to public health. Recent outbreaks in Brazil, Bolivia, and Paraguay and the observation that vectors capable of transmitting YFV are presenting in urban areas underscore the urgency of improving surveillance and diagnostic methods. Two novel methods (RT-hemi-nested-PCR and SYBR® Green qRT-PCR) for efficient detection of YFV strains circulating in South America have been developed. The methods were validated using samples obtained from golden hamsters infected experimentally with wild-type YFV strains as well as human serum and tissue samples with acute disease.
Bibliography:	http://dx.doi.org/10.1016/j.jviromet.2011.02.025 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 ObjectType-Undefined-3 ObjectType-Article-2 ObjectType-Feature-1
ISSN:	0166-0934 1879-0984 1879-0984
DOI:	10.1016/j.jviromet.2011.02.025