Different doses of methamphetamine alter long-term potentiation, level of BDNF and neuronal apoptosis in the hippocampus of reinstated rats

Methamphetamine (METH) is a psychostimulant. The precise mechanisms of its effects remain unknown and current relapse treatments have low efficacy. However, brain-derived neurotrophic factor (BDNF) and neuronal plasticity are essential contributors, despite paradoxical reports and a lack of comprehe...

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Vydané v:The journal of physiological sciences Ročník 69; číslo 2; s. 409 - 419
Hlavní autori: Shahidi, Siamak, Komaki, Alireza, Sadeghian, Reihaneh, Asl, Sara Soleimani
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: Japan Elsevier Inc 01.03.2019
BioMed Central
Springer Japan
Predmet:
Addictions
Animals
Antibodies
Apoptosis
Apoptosis - drug effects
Brain derived neurotrophic factor
Brain-Derived Neurotrophic Factor - metabolism
Central Nervous System Stimulants - pharmacology
Conditioned place preference
Drug dosages
Electrophysiology
Extinction
FDA approval
Hippocampus
Hippocampus - drug effects
Laboratory animals
Long-term potentiation
Long-Term Potentiation - drug effects
Male
Methamphetamine
Methamphetamine - pharmacology
Neurobiology
Neuronal apoptosis
Neuronal Plasticity - drug effects
Neuroplasticity
Original Paper
Place preference conditioning
Proteins
Rats
Rats, Wistar
Reinstatement
Software
Synaptic plasticity
Variance analysis
Western blotting
United States > US
Neuronal apoptosis
Brain derived neurotrophic factor
Conditioned place preference
Reinstatement
Long-term potentiation
Methamphetamine
ISSN:1880-6546, 1880-6562, 1880-6562
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Shrnutí:Methamphetamine (METH) is a psychostimulant. The precise mechanisms of its effects remain unknown and current relapse treatments have low efficacy. However, brain-derived neurotrophic factor (BDNF) and neuronal plasticity are essential contributors, despite paradoxical reports and a lack of comprehensive studies. Therefore, we investigated the effects of different doses of METH on long-term potentiation (LTP), BDNF expression and neuronal apoptosis in the hippocampus of reinstated rats. Rats were injected intraperitoneally with METH (1, 5, or 10 mg/kg) or saline, and trained in a conditioned place preference paradigm. Following implementation of the reinstatement model, electrophysiology, western blotting and TUNEL assay were performed to assess behavior, LTP components, BDNF expression, and neuronal apoptosis, respectively. The results demonstrated that the preference scores, population spike amplitude and BDNF expression markedly decreased in the METH (10 mg/kg) group compared with the other groups. In contrast, METH (5 mg/kg) significantly increased these factors more than the control group. There was no change in variables between METH (1 mg/kg) and the control group. Also, apoptosis of the hippocampus was increased in the METH (10 mg/kg) group compared with the METH (5 mg/kg) group. These results suggest that alterations in synaptic plasticity, expression of BDNF and neuronal apoptosis in the hippocampus has a vital role in the context-induced reinstatement of METH seeking.
Bibliografia:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:1880-6546
1880-6562
1880-6562
DOI:10.1007/s12576-019-00660-1