Casein kinase 1 family regulates PRR5 and TOC1 in the Arabidopsis circadian clock

The circadian clock provides organisms with the ability to adapt to daily and seasonal cycles. Eukaryotic clocks mostly rely on lineage-specific transcriptional-translational feedback loops (TTFLs). Posttranslational modifications are also crucial for clock functions in fungi and animals, but the po...

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Vydáno v:Proceedings of the National Academy of Sciences - PNAS Ročník 116; číslo 23; s. 11528
Hlavní autoři: Uehara, Takahiro N, Mizutani, Yoshiyuki, Kuwata, Keiko, Hirota, Tsuyoshi, Sato, Ayato, Mizoi, Junya, Takao, Saori, Matsuo, Hiromi, Suzuki, Takamasa, Ito, Shogo, Saito, Ami N, Nishiwaki-Ohkawa, Taeko, Yamaguchi-Shinozaki, Kazuko, Yoshimura, Takashi, Kay, Steve A, Itami, Kenichiro, Kinoshita, Toshinori, Yamaguchi, Junichiro, Nakamichi, Norihito
Médium: Journal Article
Jazyk:angličtina
Vydáno: United States 04.06.2019
Témata:
Arabidopsis - genetics
Arabidopsis Proteins - genetics
Casein Kinase I - genetics
Circadian Clocks - genetics
Circadian Rhythm - genetics
Gene Expression Regulation, Plant - genetics
Phosphorylation - genetics
Protein Processing, Post-Translational - genetics
Transcription Factors - genetics
Transcription, Genetic - genetics
small molecule
Arabidopsis
circadian clock
posttranslational regulation
ISSN:1091-6490, 1091-6490
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Shrnutí:The circadian clock provides organisms with the ability to adapt to daily and seasonal cycles. Eukaryotic clocks mostly rely on lineage-specific transcriptional-translational feedback loops (TTFLs). Posttranslational modifications are also crucial for clock functions in fungi and animals, but the posttranslational modifications that affect the plant clock are less understood. Here, using chemical biology strategies, we show that the CASEIN KINASE 1 LIKE (CKL) family is involved in posttranslational modification in the plant clock. Chemical screening demonstrated that an animal CDC7/CDK9 inhibitor, PHA767491, lengthens the circadian period. Affinity proteomics using a chemical probe revealed that PHA767491 binds to and inhibits multiple CKL proteins, rather than CDC7/CDK9 homologs. Simultaneous knockdown of CKL-encoding genes lengthened the circadian period. CKL4 phosphorylated transcriptional repressors PSEUDO-RESPONSE REGULATOR 5 (PRR5) and TIMING OF CAB EXPRESSION 1 (TOC1) in the TTFL. PHA767491 treatment resulted in accumulation of PRR5 and TOC1, accompanied by decreasing expression of PRR5- and TOC1-target genes. A double mutant was hyposensitive to PHA767491-induced period lengthening. Together, our results reveal posttranslational modification of transcriptional repressors in plant clock TTFL by CK1 family proteins, which also modulate nonplant circadian clocks.
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ISSN:1091-6490
1091-6490
DOI:10.1073/pnas.1903357116