Active uptake of dendritic cell-derived exovesicles by epithelial cells induces the release of inflammatory mediators through a TNF-alpha-mediated pathway

Dendritic cells (DCs) can release hundreds of membrane vesicles, called exovesicles, which are able to activate resting DCs and distribute antigen. Here, we examined the role of mature DC-derived exovesicles in innate and adaptive immunity, in particular their capacity to activate epithelial cells....

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Vydané v:The American journal of pathology Ročník 175; číslo 2; s. 696
Hlavní autori: Obregon, Carolina, Rothen-Rutishauser, Barbara, Gerber, Peter, Gehr, Peter, Nicod, Laurent P
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: United States 01.08.2009
Predmet:
Antigens, CD - metabolism
CD40 Antigens - metabolism
CD83 Antigen
Dendritic Cells - immunology
Dendritic Cells - metabolism
Endocytosis - immunology
Epithelial Cells - immunology
Exosomes - metabolism
Histocompatibility Antigens Class II - metabolism
Humans
Immunoglobulins - metabolism
Inflammation Mediators - metabolism
Membrane Glycoproteins - metabolism
Tumor Necrosis Factor-alpha - metabolism
ISSN:1525-2191, 1525-2191
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Abstract Dendritic cells (DCs) can release hundreds of membrane vesicles, called exovesicles, which are able to activate resting DCs and distribute antigen. Here, we examined the role of mature DC-derived exovesicles in innate and adaptive immunity, in particular their capacity to activate epithelial cells. Our analysis of exovesicle contents showed that exovesicles contain major histocompatibility complex-II, CD40, and CD83 molecules in addition to tumor necrosis factor (TNF) receptors, TNFRI and TNFRII, and are important carriers of TNF-alpha. These exovesicles are rapidly internalized by epithelial cells, inducing the release of cytokines and chemokines, but do not transfer an alloantigen-presenting capacity to epithelial cells. Part of this activation appears to involve the TNF-alpha-mediated pathway, highlighting the key role of DC-derived exovesicles, not only in adaptive immunity, but also in innate immunity by triggering innate immune responses and activating neighboring epithelial cells to release cytokines and chemokines, thereby amplifying the magnitude of the innate immune response.
AbstractList Dendritic cells (DCs) can release hundreds of membrane vesicles, called exovesicles, which are able to activate resting DCs and distribute antigen. Here, we examined the role of mature DC-derived exovesicles in innate and adaptive immunity, in particular their capacity to activate epithelial cells. Our analysis of exovesicle contents showed that exovesicles contain major histocompatibility complex-II, CD40, and CD83 molecules in addition to tumor necrosis factor (TNF) receptors, TNFRI and TNFRII, and are important carriers of TNF-alpha. These exovesicles are rapidly internalized by epithelial cells, inducing the release of cytokines and chemokines, but do not transfer an alloantigen-presenting capacity to epithelial cells. Part of this activation appears to involve the TNF-alpha-mediated pathway, highlighting the key role of DC-derived exovesicles, not only in adaptive immunity, but also in innate immunity by triggering innate immune responses and activating neighboring epithelial cells to release cytokines and chemokines, thereby amplifying the magnitude of the innate immune response.Dendritic cells (DCs) can release hundreds of membrane vesicles, called exovesicles, which are able to activate resting DCs and distribute antigen. Here, we examined the role of mature DC-derived exovesicles in innate and adaptive immunity, in particular their capacity to activate epithelial cells. Our analysis of exovesicle contents showed that exovesicles contain major histocompatibility complex-II, CD40, and CD83 molecules in addition to tumor necrosis factor (TNF) receptors, TNFRI and TNFRII, and are important carriers of TNF-alpha. These exovesicles are rapidly internalized by epithelial cells, inducing the release of cytokines and chemokines, but do not transfer an alloantigen-presenting capacity to epithelial cells. Part of this activation appears to involve the TNF-alpha-mediated pathway, highlighting the key role of DC-derived exovesicles, not only in adaptive immunity, but also in innate immunity by triggering innate immune responses and activating neighboring epithelial cells to release cytokines and chemokines, thereby amplifying the magnitude of the innate immune response.
Dendritic cells (DCs) can release hundreds of membrane vesicles, called exovesicles, which are able to activate resting DCs and distribute antigen. Here, we examined the role of mature DC-derived exovesicles in innate and adaptive immunity, in particular their capacity to activate epithelial cells. Our analysis of exovesicle contents showed that exovesicles contain major histocompatibility complex-II, CD40, and CD83 molecules in addition to tumor necrosis factor (TNF) receptors, TNFRI and TNFRII, and are important carriers of TNF-alpha. These exovesicles are rapidly internalized by epithelial cells, inducing the release of cytokines and chemokines, but do not transfer an alloantigen-presenting capacity to epithelial cells. Part of this activation appears to involve the TNF-alpha-mediated pathway, highlighting the key role of DC-derived exovesicles, not only in adaptive immunity, but also in innate immunity by triggering innate immune responses and activating neighboring epithelial cells to release cytokines and chemokines, thereby amplifying the magnitude of the innate immune response.
Author Obregon, Carolina
Rothen-Rutishauser, Barbara
Nicod, Laurent P
Gerber, Peter
Gehr, Peter
Author_xml – sequence: 1
  givenname: Carolina
  surname: Obregon
  fullname: Obregon, Carolina
  organization: Médecin chef, Service de pneumologie, CHUV, 1011 Lausanne. Switzerland
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  givenname: Barbara
  surname: Rothen-Rutishauser
  fullname: Rothen-Rutishauser, Barbara
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  givenname: Peter
  surname: Gerber
  fullname: Gerber, Peter
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  givenname: Peter
  surname: Gehr
  fullname: Gehr, Peter
– sequence: 5
  givenname: Laurent P
  surname: Nicod
  fullname: Nicod, Laurent P
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Snippet Dendritic cells (DCs) can release hundreds of membrane vesicles, called exovesicles, which are able to activate resting DCs and distribute antigen. Here, we...
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SubjectTerms Antigens, CD - metabolism
CD40 Antigens - metabolism
CD83 Antigen
Dendritic Cells - immunology
Dendritic Cells - metabolism
Endocytosis - immunology
Epithelial Cells - immunology
Exosomes - metabolism
Histocompatibility Antigens Class II - metabolism
Humans
Immunoglobulins - metabolism
Inflammation Mediators - metabolism
Membrane Glycoproteins - metabolism
Tumor Necrosis Factor-alpha - metabolism
Title Active uptake of dendritic cell-derived exovesicles by epithelial cells induces the release of inflammatory mediators through a TNF-alpha-mediated pathway
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