Lausannevirus, a giant amoebal virus encoding histone doublets

Summary Large viruses infecting algae or amoebae belong to the NucleoCytoplasmic Large DNA Viruses (NCLDV) and present genotypic and phenotypic characteristics that have raised major interest among microbiologists. Here, we describe a new large virus discovered in Acanthamoeba castellanii co‐culture...

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Published in:Environmental microbiology Vol. 13; no. 6; pp. 1454 - 1466
Main Authors: Thomas, Vincent, Bertelli, Claire, Collyn, François, Casson, Nicola, Telenti, Amalio, Goesmann, Alexander, Croxatto, Antony, Greub, Gilbert
Format: Journal Article
Language:English
Published: Oxford, UK Blackwell Publishing Ltd 01.06.2011
Subjects:
Acanthamoeba castellanii
Acanthamoeba castellanii - virology
Amoeba
Base Sequence
Biological Evolution
classification
coculture
DNA Viruses
DNA Viruses - classification
DNA Viruses - genetics
DNA Viruses - isolation & purification
genes
genetics
Genome, Viral
histones
Histones - genetics
host range
Host Specificity
isolation & purification
Mimivirus
Molecular Sequence Data
Phylogeny
Rivers
Rivers - virology
virology
viruses
Water Microbiology
ISSN:1462-2912, 1462-2920, 1462-2920
Online Access:Get full text
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Summary:Summary Large viruses infecting algae or amoebae belong to the NucleoCytoplasmic Large DNA Viruses (NCLDV) and present genotypic and phenotypic characteristics that have raised major interest among microbiologists. Here, we describe a new large virus discovered in Acanthamoeba castellanii co‐culture of an environmental sample. The virus, referred to as Lausannevirus, has a very limited host range, infecting Acanthamoeba spp. but being unable to infect other amoebae and mammalian cell lines tested. Within A. castellanii, this icosahedral virus of about 200 nm exhibits a development cycle similar to Mimivirus, with an eclipse phase 2 h post infection and a logarithmic growth leading to amoebal lysis in less than 24 h. The 346 kb Lausannevirus genome presents similarities with the recently described Marseillevirus, sharing 89% of genes, and thus belongs to the same family as confirmed by core gene phylogeny. Interestingly, Lausannevirus and Marseillevirus genomes both encode three proteins with predicted histone folds, including two histone doublets, that present similarities to eukaryotic and archaeal histones. The discovery of Lausannevirus and the analysis of its genome provide some insight in the evolution of these large amoebae‐infecting viruses.
Bibliography:ark:/67375/WNG-KKVFP1RK-5
ArticleID:EMI2446
istex:D423FCB169DEFA6B3331150631C35D9DF6439978
Equal contribution.
Present address: STERIS SA R&D – 18, Route du Panorama, 92260 Fontenay‐aux‐Roses, France.
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ISSN:1462-2912
1462-2920
1462-2920
DOI:10.1111/j.1462-2920.2011.02446.x