Isolation of skeletal muscle stem cells by fluorescence-activated cell sorting

In this protocol, limb muscles are physically and enzymatically dissociated to maximally release resident mononucleated cells. Pure populations of either quiescent or activated muscle stem cells are then isolated by flow cytometry. The prospective isolation of purified stem cell populations has dram...

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Bibliographic Details
Published in:	Nature protocols Vol. 10; no. 10; pp. 1612 - 1624
Main Authors:	Liu, Ling, Cheung, Tom H, Charville, Gregory W, Rando, Thomas A
Format:	Journal Article
Language:	English
Published:	London Nature Publishing Group UK 01.10.2015 Nature Publishing Group
Subjects:	631/1647/1407/1492 631/532/2439 Analytical Chemistry Animals Biological Techniques Cell separation Computational Biology/Bioinformatics Cytological Techniques - methods Flow Cytometry Fluorescence Genetic Markers - genetics Hindlimb - cytology Leukocytes, Mononuclear - cytology Life Sciences Methods Mice Microarrays Muscle, Skeletal - cytology Muscles Organic Chemistry Physiological aspects protocol Stem cells Stem Cells - cytology United States
ISSN:	1754-2189, 1750-2799, 1750-2799
Online Access:	Get full text
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