In situ cultivation of previously uncultivable microorganisms using the ichip

This protocol details the construction and use of the ichip, a platform developed to isolate previously uncultivable microorganisms from a range of environmental samples, by enabling exposure to natural growth factors through in situ culture. Most microbial species remain uncultivated, and modifying...

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Bibliographic Details
Published in:	Nature protocols Vol. 12; no. 10; pp. 2232 - 2242
Main Authors:	Berdy, Brittany, Spoering, Amy L, Ling, Losee L, Epstein, Slava S
Format:	Journal Article
Language:	English
Published:	London Nature Publishing Group UK 01.10.2017 Nature Publishing Group
Subjects:	631/154 631/1647/2234 631/326/171 631/326/41 631/326/41/2537 Analytical Chemistry Bacteria Bacteria - cytology Bacteria - metabolism Bacteriological Techniques - instrumentation Biochemistry & Molecular Biology Biological Techniques Cell culture Cell Culture Techniques - instrumentation Cell Culture Techniques - methods Chambers Colonies Computational Biology/Bioinformatics Construction Cultivation Culture media Culture techniques Diffusion chambers Dilution Domestication Environmental Microbiology Equipment Design Germfree Growth factors Harvesting Humans Innovations Life Sciences Microarray Analysis - instrumentation Microarrays Microorganisms Miniaturization Mouth - microbiology Organic Chemistry protocol Pure culture Soil bacteria Soil microorganisms
ISSN:	1754-2189, 1750-2799, 1750-2799
Online Access:	Get full text
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