Local externalization of phosphatidylserine mediates developmental synaptic pruning by microglia

Neuronal circuit assembly requires the fine balance between synapse formation and elimination. Microglia, through the elimination of supernumerary synapses, have an established role in this process. While the microglial receptor TREM2 and the soluble complement proteins C1q and C3 are recognized as...

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Veröffentlicht in:	The EMBO journal Jg. 39; H. 16; S. e105380 - n/a
Hauptverfasser:	Scott‐Hewitt, Nicole, Perrucci, Fabio, Morini, Raffaella, Erreni, Marco, Mahoney, Matthew, Witkowska, Agata, Carey, Alanna, Faggiani, Elisa, Schuetz, Lisa Theresia, Mason, Sydney, Tamborini, Matteo, Bizzotto, Matteo, Passoni, Lorena, Filipello, Fabia, Jahn, Reinhard, Stevens, Beth, Matteoli, Michela
Format:	Journal Article
Sprache:	Englisch
Veröffentlicht:	London Nature Publishing Group UK 17.08.2020 Springer Nature B.V John Wiley and Sons Inc
Schlagworte:	Animals Annexin V C1q Caspase-3 Circuits Coculture Techniques Complement C1q - genetics Complement C1q - metabolism Complement C3 - genetics Complement C3 - metabolism Complement component C1q Complement component C3 EMBO19 EMBO27 Exposure Hippocampus Hippocampus - metabolism Membrane Glycoproteins - genetics Membrane Glycoproteins - metabolism Mice Mice, Knockout Microglia Microglia - metabolism Neural networks Neurons - metabolism Phosphatidylserine Phosphatidylserines - genetics Phosphatidylserines - metabolism Pruning Receptors, Immunologic - genetics Receptors, Immunologic - metabolism Supernumerary Synapse elimination synapse pruning Synapses Synapses - genetics Synapses - metabolism Synaptogenesis TREM2 Visual pathways Visual system phosphatidylserine synapse pruning C1q TREM2 microglia
ISSN:	0261-4189, 1460-2075, 1460-2075
Online-Zugang:	Volltext
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Zusammenfassung:	Neuronal circuit assembly requires the fine balance between synapse formation and elimination. Microglia, through the elimination of supernumerary synapses, have an established role in this process. While the microglial receptor TREM2 and the soluble complement proteins C1q and C3 are recognized as key players, the neuronal molecular components that specify synapses to be eliminated are still undefined. Here, we show that exposed phosphatidylserine (PS) represents a neuronal “eat‐me” signal involved in microglial‐mediated pruning. In hippocampal neuron and microglia co‐cultures, synapse elimination can be partially prevented by blocking accessibility of exposed PS using Annexin V or through microglial loss of TREM2. In vivo , PS exposure at both hippocampal and retinogeniculate synapses and engulfment of PS‐labeled material by microglia occurs during established developmental periods of microglial‐mediated synapse elimination. Mice deficient in C1q, which fail to properly refine retinogeniculate connections, have elevated presynaptic PS exposure and reduced PS engulfment by microglia. These data provide mechanistic insight into microglial‐mediated synapse pruning and identify a novel role of developmentally regulated neuronal PS exposure that is common among developing brain structures. Synopsis Microglia help refine developing neural circuits through the elimination of supernumerary synapses. Here we show that exposed phosphatidylserine on pre‐ and postsynaptic membranes functions as an “eat‐me” signal contributing to microglial‐mediated synapse pruning. Phosphatidylserine exposure at both hippocampal and retinogeniculate synapses coincides with the onset of synapse elimination and PS engulfment by microglia. Microglia‐mediated synapse elimination is dependent on TREM2 and exposed phosphatidylserine in vitro . Exposed phosphatidylserine is developmentally regulated across periods of pruning in both hippocampus and visual system. In vivo developmental phosphatidylserine exposure is not caspase 3‐dependent. Loss of C1q leads to elevated phosphatidylserine‐positive presynaptic inputs and reduced microglia engulfment. Graphical Abstract Exposed phosphatidylserine on pre‐ and postsynaptic membranes functions as an “eat‐me” signal contributing to microglia‐mediated synapse pruning.
Bibliographie:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 These authors contributed equally to this work See also: G Peet et al (August 2020)
ISSN:	0261-4189 1460-2075 1460-2075
DOI:	10.15252/embj.2020105380