Light signaling controls nuclear architecture reorganization during seedling establishment

The spatial organization of chromatin can be subject to extensive remodeling in plant somatic cells in response to developmental and environmental signals. However, the mechanisms controlling these dynamic changes and their functional impact on nuclear activity are poorly understood. Here, we determ...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS Vol. 112; no. 21; pp. E2836 - E2844
Main Authors: Bourbousse, Clara, Mestiri, Imen, Zabulon, Gerald, Bourge, Mickaël, Formiggini, Fabio, Koini, Maria A, Brown, Spencer C, Fransz, Paul, Bowler, Chris, Barneche, Fredy
Format: Journal Article
Language:English
Published: United States 26.05.2015
Subjects:
Arabidopsis - genetics
Arabidopsis - growth & development
Arabidopsis - radiation effects
Arabidopsis Proteins - genetics
Arabidopsis Proteins - metabolism
Cell Nucleus - genetics
Cell Nucleus - metabolism
Cell Nucleus - radiation effects
Chromatin Assembly and Disassembly - genetics
Chromatin Assembly and Disassembly - radiation effects
Cotyledon - growth & development
Cotyledon - metabolism
Cotyledon - radiation effects
DNA Methylation
Gene Silencing
Genes, Plant
Heterochromatin - genetics
Heterochromatin - radiation effects
Light Signal Transduction - genetics
Nuclear Proteins - genetics
Nuclear Proteins - metabolism
Plants, Genetically Modified
RNA Polymerase II - metabolism
Seedlings - growth & development
Seedlings - metabolism
Seedlings - radiation effects
Ubiquitin-Protein Ligases - genetics
Ubiquitin-Protein Ligases - metabolism
plant development
photomorphogenesis
nuclear organization
light signaling
heterochromatin
ISSN:1091-6490
Online Access:Get more information
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Summary:The spatial organization of chromatin can be subject to extensive remodeling in plant somatic cells in response to developmental and environmental signals. However, the mechanisms controlling these dynamic changes and their functional impact on nuclear activity are poorly understood. Here, we determined that light perception triggers a switch between two different nuclear architectural schemes during Arabidopsis postembryonic development. Whereas progressive nucleus expansion and heterochromatin rearrangements in cotyledon cells are achieved similarly under light and dark conditions during germination, the later steps that lead to mature nuclear phenotypes are intimately associated with the photomorphogenic transition in an organ-specific manner. The light signaling integrators DE-ETIOLATED 1 and CONSTITUTIVE PHOTOMORPHOGENIC 1 maintain heterochromatin in a decondensed state in etiolated cotyledons. In contrast, under light conditions cryptochrome-mediated photoperception releases nuclear expansion and heterochromatin compaction within conspicuous chromocenters. For all tested loci, chromatin condensation during photomorphogenesis does not detectably rely on DNA methylation-based processes. Notwithstanding, the efficiency of transcriptional gene silencing may be impacted during the transition, as based on the reactivation of transposable element-driven reporter genes. Finally, we report that global engagement of RNA polymerase II in transcription is highly increased under light conditions, suggesting that cotyledon photomorphogenesis involves a transition from globally quiescent to more active transcriptional states. Given these findings, we propose that light-triggered changes in nuclear architecture underlie interplays between heterochromatin reorganization and transcriptional reprogramming associated with the establishment of photosynthesis.
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ISSN:1091-6490
DOI:10.1073/pnas.1503512112