Structure of the bluetongue virus capsid

Seven polypeptides were found to be present in the capsid of the bluetongue virus (BTV), four of which are major and three are minor components. This number and size distribution is the same as that found in reovirus, which has a similar segmented, double-stranded ribonucleic acid genome. In both vi...

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Vydáno v:Journal of virology Ročník 10; číslo 4; s. 783
Hlavní autoři: Verwoerd, D W, Els, H J, De Villiers, E M, Huismans, H
Médium: Journal Article
Jazyk:angličtina
Vydáno: United States 01.10.1972
Témata:
Animals
Bluetongue - microbiology
Cell Line
Centrifugation, Density Gradient
Cricetinae
Densitometry
DNA-Directed RNA Polymerases - metabolism
Electrophoresis, Polyacrylamide Gel
Kidney
Microscopy, Electron
Molecular Weight
Peptides - analysis
Reoviridae - analysis
RNA Viruses - analysis
RNA Viruses - enzymology
RNA Viruses - growth & development
Sheep
Sheep Diseases - microbiology
Staining and Labeling
Tritium
Uracil Nucleotides - metabolism
Viral Proteins - analysis
Virus Diseases - veterinary
ISSN:0022-538X
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Popis
Shrnutí:Seven polypeptides were found to be present in the capsid of the bluetongue virus (BTV), four of which are major and three are minor components. This number and size distribution is the same as that found in reovirus, which has a similar segmented, double-stranded ribonucleic acid genome. In both viruses an excellent correlation is found between the molecular weights of certain genome segments and those of the polypeptides, suggesting a direct coding relationship between them. Careful comparison, however, indicates that the correlation patterns of the two viruses are different, suggesting that different genome segments may be utilized to code for certain capsid proteins. This phenomenon may be related to the fact that these viruses can be distinguished morphologically, reovirus having a larger diameter and possessing a double-layered capsid against the smaller single capsid of the BTV. Utilizing the degrading effect of CsCl gradients, it was demonstrated, however, that two of the BTV polypeptides are present as a diffuse protein layer surrounding the capsid. This outer layer probably has some of the functions of the reovirus outer capsid, one being the "masking" of a viral transcriptase which could be demonstrated after its removal.
Bibliografie:ObjectType-Article-1
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ISSN:0022-538X
DOI:10.1128/jvi.10.4.783-794.1972