Relaxed Selection Drives a Noisy Noncoding Transcriptome in Members of the Mycobacterium tuberculosis Complex
Related species are often used to understand the molecular underpinning of virulence through examination of a shared set of biological features attributable to a core genome of orthologous genes. An important but insufficiently studied issue, however, is the extent to which the regulatory architectu...
Gespeichert in:
| Veröffentlicht in: | mBio Jg. 5; H. 4; S. e01169 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Journal Article |
| Sprache: | Englisch |
| Veröffentlicht: |
United States
American Society for Microbiology
05.08.2014
American Society of Microbiology |
| Schlagworte: | |
| ISSN: | 2161-2129, 2150-7511, 2150-7511 |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| Abstract | Related species are often used to understand the molecular underpinning of virulence through examination of a shared set of biological features attributable to a core genome of orthologous genes. An important but insufficiently studied issue, however, is the extent to which the regulatory architectures are similarly conserved. A small number of studies have compared the primary transcriptomes of different bacterial species, but few have compared closely related species with clearly divergent evolutionary histories. We addressed the impact of differing modes of evolution within the genus
Mycobacterium
through comparison of the primary transcriptome of
M. marinum
with that of a closely related lineage,
M. bovis
. Both are thought to have evolved from an ancestral generalist species, with
M. bovis
and other members of the
M. tuberculosis
complex having subsequently undergone downsizing of their genomes during the transition to obligate pathogenicity.
M. marinum
, in contrast, has retained a large genome, appropriate for an environmental organism, and is a broad-host-range pathogen. We also examined changes over a shorter evolutionary time period through comparison of the primary transcriptome of
M. bovis
with that of another member of the
M. tuberculosis
complex (
M. tuberculosis
) which possesses an almost identical genome but maintains a distinct host preference.
IMPORTANCE
Our comparison of the transcriptional start site (TSS) maps of
M. marinum
and
M. bovis
uncovers a pillar of conserved promoters, noncoding RNA (NCRNA), and a genome-wide signal in the −35 promoter regions of both species. We identify evolutionarily conserved transcriptional attenuation and highlight its potential contribution to multidrug resistance mediated through the transcriptional regulator
whiB7
. We show that a species population history is reflected in its transcriptome and posit relaxed selection as the main driver of an abundance of canonical −10 promoter sites in
M. bovis
relative to
M. marinum
. It appears that transcriptome composition in mycobacteria is driven primarily by the availability of such sites and that their frequencies diverge significantly across the mycobacterial clade. Finally, through comparison of
M. bovis
and
M. tuberculosis
, we illustrate that single nucleotide polymorphism (SNP)-driven promoter differences likely underpin many of the transcriptional differences between
M. tuberculosis
complex lineages.
Our comparison of the transcriptional start site (TSS) maps of
M. marinum
and
M. bovis
uncovers a pillar of conserved promoters, noncoding RNA (NCRNA), and a genome-wide signal in the −35 promoter regions of both species. We identify evolutionarily conserved transcriptional attenuation and highlight its potential contribution to multidrug resistance mediated through the transcriptional regulator
whiB7
. We show that a species population history is reflected in its transcriptome and posit relaxed selection as the main driver of an abundance of canonical −10 promoter sites in
M. bovis
relative to
M. marinum
. It appears that transcriptome composition in mycobacteria is driven primarily by the availability of such sites and that their frequencies diverge significantly across the mycobacterial clade. Finally, through comparison of
M. bovis
and
M. tuberculosis
, we illustrate that single nucleotide polymorphism (SNP)-driven promoter differences likely underpin many of the transcriptional differences between
M. tuberculosis
complex lineages. |
|---|---|
| AbstractList | Related species are often used to understand the molecular underpinning of virulence through examination of a shared set of biological features attributable to a core genome of orthologous genes. An important but insufficiently studied issue, however, is the extent to which the regulatory architectures are similarly conserved. A small number of studies have compared the primary transcriptomes of different bacterial species, but few have compared closely related species with clearly divergent evolutionary histories. We addressed the impact of differing modes of evolution within the genus Mycobacterium through comparison of the primary transcriptome of M. marinum with that of a closely related lineage, M. bovis. Both are thought to have evolved from an ancestral generalist species, with M. bovis and other members of the M. tuberculosis complex having subsequently undergone downsizing of their genomes during the transition to obligate pathogenicity. M. marinum, in contrast, has retained a large genome, appropriate for an environmental organism, and is a broad-host-range pathogen. We also examined changes over a shorter evolutionary time period through comparison of the primary transcriptome of M. bovis with that of another member of the M. tuberculosis complex (M. tuberculosis) which possesses an almost identical genome but maintains a distinct host preference. Our comparison of the transcriptional start site (TSS) maps of M. marinum and M. bovis uncovers a pillar of conserved promoters, noncoding RNA (NCRNA), and a genome-wide signal in the −35 promoter regions of both species. We identify evolutionarily conserved transcriptional attenuation and highlight its potential contribution to multidrug resistance mediated through the transcriptional regulator whiB7. We show that a species population history is reflected in its transcriptome and posit relaxed selection as the main driver of an abundance of canonical −10 promoter sites in M. bovis relative to M. marinum. It appears that transcriptome composition in mycobacteria is driven primarily by the availability of such sites and that their frequencies diverge significantly across the mycobacterial clade. Finally, through comparison of M. bovis and M. tuberculosis, we illustrate that single nucleotide polymorphism (SNP)-driven promoter differences likely underpin many of the transcriptional differences between M. tuberculosis complex lineages. Related species are often used to understand the molecular underpinning of virulence through examination of a shared set of biological features attributable to a core genome of orthologous genes. An important but insufficiently studied issue, however, is the extent to which the regulatory architectures are similarly conserved. A small number of studies have compared the primary transcriptomes of different bacterial species, but few have compared closely related species with clearly divergent evolutionary histories. We addressed the impact of differing modes of evolution within the genus Mycobacterium through comparison of the primary transcriptome of M. marinum with that of a closely related lineage, M. bovis. Both are thought to have evolved from an ancestral generalist species, with M. bovis and other members of the M. tuberculosis complex having subsequently undergone downsizing of their genomes during the transition to obligate pathogenicity. M. marinum, in contrast, has retained a large genome, appropriate for an environmental organism, and is a broad-host-range pathogen. We also examined changes over a shorter evolutionary time period through comparison of the primary transcriptome of M. bovis with that of another member of the M. tuberculosis complex (M. tuberculosis) which possesses an almost identical genome but maintains a distinct host preference. Importance: Our comparison of the transcriptional start site (TSS) maps of M. marinum and M. bovis uncovers a pillar of conserved promoters, noncoding RNA (NCRNA), and a genome-wide signal in the -35 promoter regions of both species. We identify evolutionarily conserved transcriptional attenuation and highlight its potential contribution to multidrug resistance mediated through the transcriptional regulator whiB7. We show that a species population history is reflected in its transcriptome and posit relaxed selection as the main driver of an abundance of canonical -10 promoter sites in M. bovis relative to M. marinum. It appears that transcriptome composition in mycobacteria is driven primarily by the availability of such sites and that their frequencies diverge significantly across the mycobacterial clade. Finally, through comparison of M. bovis and M. tuberculosis, we illustrate that single nucleotide polymorphism (SNP)-driven promoter differences likely underpin many of the transcriptional differences between M. tuberculosis complex lineages.Related species are often used to understand the molecular underpinning of virulence through examination of a shared set of biological features attributable to a core genome of orthologous genes. An important but insufficiently studied issue, however, is the extent to which the regulatory architectures are similarly conserved. A small number of studies have compared the primary transcriptomes of different bacterial species, but few have compared closely related species with clearly divergent evolutionary histories. We addressed the impact of differing modes of evolution within the genus Mycobacterium through comparison of the primary transcriptome of M. marinum with that of a closely related lineage, M. bovis. Both are thought to have evolved from an ancestral generalist species, with M. bovis and other members of the M. tuberculosis complex having subsequently undergone downsizing of their genomes during the transition to obligate pathogenicity. M. marinum, in contrast, has retained a large genome, appropriate for an environmental organism, and is a broad-host-range pathogen. We also examined changes over a shorter evolutionary time period through comparison of the primary transcriptome of M. bovis with that of another member of the M. tuberculosis complex (M. tuberculosis) which possesses an almost identical genome but maintains a distinct host preference. Importance: Our comparison of the transcriptional start site (TSS) maps of M. marinum and M. bovis uncovers a pillar of conserved promoters, noncoding RNA (NCRNA), and a genome-wide signal in the -35 promoter regions of both species. We identify evolutionarily conserved transcriptional attenuation and highlight its potential contribution to multidrug resistance mediated through the transcriptional regulator whiB7. We show that a species population history is reflected in its transcriptome and posit relaxed selection as the main driver of an abundance of canonical -10 promoter sites in M. bovis relative to M. marinum. It appears that transcriptome composition in mycobacteria is driven primarily by the availability of such sites and that their frequencies diverge significantly across the mycobacterial clade. Finally, through comparison of M. bovis and M. tuberculosis, we illustrate that single nucleotide polymorphism (SNP)-driven promoter differences likely underpin many of the transcriptional differences between M. tuberculosis complex lineages. Related species are often used to understand the molecular underpinning of virulence through examination of a shared set of biological features attributable to a core genome of orthologous genes. An important but insufficiently studied issue, however, is the extent to which the regulatory architectures are similarly conserved. A small number of studies have compared the primary transcriptomes of different bacterial species, but few have compared closely related species with clearly divergent evolutionary histories. We addressed the impact of differing modes of evolution within the genus Mycobacterium through comparison of the primary transcriptome of M. marinum with that of a closely related lineage, M. bovis . Both are thought to have evolved from an ancestral generalist species, with M. bovis and other members of the M. tuberculosis complex having subsequently undergone downsizing of their genomes during the transition to obligate pathogenicity. M. marinum , in contrast, has retained a large genome, appropriate for an environmental organism, and is a broad-host-range pathogen. We also examined changes over a shorter evolutionary time period through comparison of the primary transcriptome of M. bovis with that of another member of the M. tuberculosis complex ( M. tuberculosis ) which possesses an almost identical genome but maintains a distinct host preference. IMPORTANCE Our comparison of the transcriptional start site (TSS) maps of M. marinum and M. bovis uncovers a pillar of conserved promoters, noncoding RNA (NCRNA), and a genome-wide signal in the −35 promoter regions of both species. We identify evolutionarily conserved transcriptional attenuation and highlight its potential contribution to multidrug resistance mediated through the transcriptional regulator whiB7 . We show that a species population history is reflected in its transcriptome and posit relaxed selection as the main driver of an abundance of canonical −10 promoter sites in M. bovis relative to M. marinum . It appears that transcriptome composition in mycobacteria is driven primarily by the availability of such sites and that their frequencies diverge significantly across the mycobacterial clade. Finally, through comparison of M. bovis and M. tuberculosis , we illustrate that single nucleotide polymorphism (SNP)-driven promoter differences likely underpin many of the transcriptional differences between M. tuberculosis complex lineages. Our comparison of the transcriptional start site (TSS) maps of M. marinum and M. bovis uncovers a pillar of conserved promoters, noncoding RNA (NCRNA), and a genome-wide signal in the −35 promoter regions of both species. We identify evolutionarily conserved transcriptional attenuation and highlight its potential contribution to multidrug resistance mediated through the transcriptional regulator whiB7 . We show that a species population history is reflected in its transcriptome and posit relaxed selection as the main driver of an abundance of canonical −10 promoter sites in M. bovis relative to M. marinum . It appears that transcriptome composition in mycobacteria is driven primarily by the availability of such sites and that their frequencies diverge significantly across the mycobacterial clade. Finally, through comparison of M. bovis and M. tuberculosis , we illustrate that single nucleotide polymorphism (SNP)-driven promoter differences likely underpin many of the transcriptional differences between M. tuberculosis complex lineages. Related species are often used to understand the molecular underpinning of virulence through examination of a shared set of biological features attributable to a core genome of orthologous genes. An important but insufficiently studied issue, however, is the extent to which the regulatory architectures are similarly conserved. A small number of studies have compared the primary transcriptomes of different bacterial species, but few have compared closely related species with clearly divergent evolutionary histories. We addressed the impact of differing modes of evolution within the genus Mycobacterium through comparison of the primary transcriptome of M. marinum with that of a closely related lineage, M. bovis. Both are thought to have evolved from an ancestral generalist species, with M. bovis and other members of the M. tuberculosis complex having subsequently undergone downsizing of their genomes during the transition to obligate pathogenicity. M. marinum, in contrast, has retained a large genome, appropriate for an environmental organism, and is a broad-host-range pathogen. We also examined changes over a shorter evolutionary time period through comparison of the primary transcriptome of M. bovis with that of another member of the M. tuberculosis complex (M. tuberculosis) which possesses an almost identical genome but maintains a distinct host preference.IMPORTANCE Our comparison of the transcriptional start site (TSS) maps of M. marinum and M. bovis uncovers a pillar of conserved promoters, noncoding RNA (NCRNA), and a genome-wide signal in the −35 promoter regions of both species. We identify evolutionarily conserved transcriptional attenuation and highlight its potential contribution to multidrug resistance mediated through the transcriptional regulator whiB7. We show that a species population history is reflected in its transcriptome and posit relaxed selection as the main driver of an abundance of canonical −10 promoter sites in M. bovis relative to M. marinum. It appears that transcriptome composition in mycobacteria is driven primarily by the availability of such sites and that their frequencies diverge significantly across the mycobacterial clade. Finally, through comparison of M. bovis and M. tuberculosis, we illustrate that single nucleotide polymorphism (SNP)-driven promoter differences likely underpin many of the transcriptional differences between M. tuberculosis complex lineages. ABSTRACT Related species are often used to understand the molecular underpinning of virulence through examination of a shared set of biological features attributable to a core genome of orthologous genes. An important but insufficiently studied issue, however, is the extent to which the regulatory architectures are similarly conserved. A small number of studies have compared the primary transcriptomes of different bacterial species, but few have compared closely related species with clearly divergent evolutionary histories. We addressed the impact of differing modes of evolution within the genus Mycobacterium through comparison of the primary transcriptome of M. marinum with that of a closely related lineage, M. bovis. Both are thought to have evolved from an ancestral generalist species, with M. bovis and other members of the M. tuberculosis complex having subsequently undergone downsizing of their genomes during the transition to obligate pathogenicity. M. marinum, in contrast, has retained a large genome, appropriate for an environmental organism, and is a broad-host-range pathogen. We also examined changes over a shorter evolutionary time period through comparison of the primary transcriptome of M. bovis with that of another member of the M. tuberculosis complex (M. tuberculosis) which possesses an almost identical genome but maintains a distinct host preference. IMPORTANCE Our comparison of the transcriptional start site (TSS) maps of M. marinum and M. bovis uncovers a pillar of conserved promoters, noncoding RNA (NCRNA), and a genome-wide signal in the −35 promoter regions of both species. We identify evolutionarily conserved transcriptional attenuation and highlight its potential contribution to multidrug resistance mediated through the transcriptional regulator whiB7. We show that a species population history is reflected in its transcriptome and posit relaxed selection as the main driver of an abundance of canonical −10 promoter sites in M. bovis relative to M. marinum. It appears that transcriptome composition in mycobacteria is driven primarily by the availability of such sites and that their frequencies diverge significantly across the mycobacterial clade. Finally, through comparison of M. bovis and M. tuberculosis, we illustrate that single nucleotide polymorphism (SNP)-driven promoter differences likely underpin many of the transcriptional differences between M. tuberculosis complex lineages. Related species are often used to understand the molecular underpinning of virulence through examination of a shared set of biological features attributable to a core genome of orthologous genes. An important but insufficiently studied issue, however, is the extent to which the regulatory architectures are similarly conserved. A small number of studies have compared the primary transcriptomes of different bacterial species, but few have compared closely related species with clearly divergent evolutionary histories. We addressed the impact of differing modes of evolution within the genus Mycobacterium through comparison of the primary transcriptome of M. marinum with that of a closely related lineage, M. bovis. Both are thought to have evolved from an ancestral generalist species, with M. bovis and other members of the M. tuberculosis complex having subsequently undergone downsizing of their genomes during the transition to obligate pathogenicity. M. marinum, in contrast, has retained a large genome, appropriate for an environmental organism, and is a broad-host-range pathogen. We also examined changes over a shorter evolutionary time period through comparison of the primary transcriptome of M. bovis with that of another member of the M. tuberculosis complex (M. tuberculosis) which possesses an almost identical genome but maintains a distinct host preference. Importance: Our comparison of the transcriptional start site (TSS) maps of M. marinum and M. bovis uncovers a pillar of conserved promoters, noncoding RNA (NCRNA), and a genome-wide signal in the -35 promoter regions of both species. We identify evolutionarily conserved transcriptional attenuation and highlight its potential contribution to multidrug resistance mediated through the transcriptional regulator whiB7. We show that a species population history is reflected in its transcriptome and posit relaxed selection as the main driver of an abundance of canonical -10 promoter sites in M. bovis relative to M. marinum. It appears that transcriptome composition in mycobacteria is driven primarily by the availability of such sites and that their frequencies diverge significantly across the mycobacterial clade. Finally, through comparison of M. bovis and M. tuberculosis, we illustrate that single nucleotide polymorphism (SNP)-driven promoter differences likely underpin many of the transcriptional differences between M. tuberculosis complex lineages. |
| Author | Conlon, Kevin M. Dinan, Adam M. Lohan, Amanda J. Malone, Kerri M. Gordon, Stephen V. Miranda-CasoLuengo, Aleksandra A. Loftus, Brendan J. Tong, Pin |
| Author_xml | – sequence: 1 givenname: Adam M. surname: Dinan fullname: Dinan, Adam M. organization: UCD Conway Institute, University College Dublin, Dublin, Ireland, School of Medicine and Medical Science, UCD Conway Institute, University College Dublin, Dublin, Ireland – sequence: 2 givenname: Pin surname: Tong fullname: Tong, Pin organization: Wellcome Trust Cell Biology Centre, The University of Edinburgh, Edinburgh, United Kingdom – sequence: 3 givenname: Amanda J. surname: Lohan fullname: Lohan, Amanda J. organization: UCD Conway Institute, University College Dublin, Dublin, Ireland – sequence: 4 givenname: Kevin M. surname: Conlon fullname: Conlon, Kevin M. organization: UCD School of Veterinary Medicine, University College Dublin, Dublin, Ireland – sequence: 5 givenname: Aleksandra A. surname: Miranda-CasoLuengo fullname: Miranda-CasoLuengo, Aleksandra A. organization: UCD Conway Institute, University College Dublin, Dublin, Ireland, School of Medicine and Medical Science, UCD Conway Institute, University College Dublin, Dublin, Ireland – sequence: 6 givenname: Kerri M. surname: Malone fullname: Malone, Kerri M. organization: UCD School of Veterinary Medicine, University College Dublin, Dublin, Ireland – sequence: 7 givenname: Stephen V. surname: Gordon fullname: Gordon, Stephen V. organization: UCD Conway Institute, University College Dublin, Dublin, Ireland, UCD School of Veterinary Medicine, University College Dublin, Dublin, Ireland, School of Medicine and Medical Science, UCD Conway Institute, University College Dublin, Dublin, Ireland – sequence: 8 givenname: Brendan J. surname: Loftus fullname: Loftus, Brendan J. organization: School of Medicine and Medical Science, UCD Conway Institute, University College Dublin, Dublin, Ireland |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/25096875$$D View this record in MEDLINE/PubMed |
| BookMark | eNqFkk1v3CAQhq0qVfPRHHutkHrpxSkDGPClUrv9ipS0UpueEcazG1a22YAdZf99cDapmqhSOQBi3nkYhvew2BvCgEXxCugJANPv-o8-nFAAWZcgnhUHDCpaqgpgb95LKBmwer84TmlN8-AcNKcvin1W0VpqVR0U_U_s7A225Bd26EYfBvIp-mtMxJLvwadtngcXWj-syEW0Q3LRb8bQI_EDOce-wZhIWJLxEsn51oXGuhGjn3oyTjnmpi4kn8gi9JsOb14Wz5e2S3h8vx4Vv798vlh8K89-fD1dfDgrXaXFWDaKUlYJAGwAW6eYssppAN0KLpVWPMtorRVVgkkuLRUOa8GQUUsrcJofFac7bhvs2myi723cmmC9uTsIcWVsHL3r0LTLRihgwLVlgnNma5DYMEaltNA6kVnvd6zN1PS5GhzGaLtH0MeRwV-aVbg2Iv8QryAD3t4DYriaMI2m98lh19kBw5QMKCEFlVyo_0urCjSjumJZ-uaJdB2mOOSuGs5mWK3ZfPfrv4v_U_WDAbKA7wQuhpQiLo3zo519kN_iOwPUzE4zs9PMndMMzD0pn2Q9gP-tvwXUv9OB |
| CitedBy_id | crossref_primary_10_1002_mlf2_70025 crossref_primary_10_1038_srep28628 crossref_primary_10_1111_mmi_13890 crossref_primary_10_1371_journal_pgen_1007282 crossref_primary_10_1016_j_micres_2020_126674 crossref_primary_10_3389_fmicb_2019_00591 crossref_primary_10_1016_j_csbj_2024_10_042 crossref_primary_10_1038_s41467_019_11948_6 crossref_primary_10_1016_j_bbamcr_2024_119787 crossref_primary_10_1016_j_jmb_2020_05_003 crossref_primary_10_1186_s12864_016_2868_y crossref_primary_10_1111_mmi_14862 crossref_primary_10_1111_mmi_14917 crossref_primary_10_1038_s41598_018_22237_5 crossref_primary_10_1186_s12864_021_07572_8 crossref_primary_10_3390_pathogens13121041 crossref_primary_10_1111_mmi_14834 |
| Cites_doi | 10.1186/1471-2105-10-154 10.1038/35059006 10.1046/j.1365-2958.1997.6361999.x 10.1074/jbc.M111.302588 10.1128/IAI.01495-08 10.1093/nar/gkq454 10.1101/gr.092759.109 10.1038/nrmicro1472 10.1371/journal.ppat.1002342 10.1371/journal.pone.0074209 10.1128/JB.05947-11 10.1186/gb-2011-12-10-r98 10.1261/rna.034116.112 10.1186/1471-2164-14-710 10.1038/nature08756 10.1371/journal.pgen.1002867 10.1146/annurev.micro.62.081307.162832 10.1128/mBio.00156-12 10.1128/AAC.02191-12 10.1101/gr.075069.107 10.1073/pnas.0505446102 10.1371/journal.pone.0079411 10.1016/j.molcel.2013.10.013 10.1371/journal.pone.0051950 10.1016/j.tim.2010.12.008 10.1016/j.tube.2012.11.012 10.1016/j.celrep.2013.10.031 10.1111/j.1365-2958.2008.06576.x 10.1073/pnas.1315974111 10.1073/pnas.1130426100 10.1099/mic.0.2007/009894-0 10.1186/1471-2164-14-145 10.1093/gbe/evt138 10.1093/nar/gkq101 10.1371/journal.pbio.0060311 10.1371/journal.pgen.1001115 10.1038/nmeth.1226 10.1186/gb-2010-11-9-r97 10.1186/gb-2009-10-3-r25 10.1016/j.tube.2010.08.003 10.1101/gr.129544.111 10.1016/j.molcel.2008.02.026 10.1074/jbc.M113.516385 10.1016/j.tube.2007.02.004 10.1016/j.ijmm.2013.09.010 10.1038/nmeth.2646 10.1371/journal.pone.0000745 10.1186/1471-2164-13-120 10.1016/j.molcel.2011.08.022 10.1126/science.1089370 10.1038/ncomms4501 10.1093/bioinformatics/btm404 |
| ContentType | Journal Article |
| Copyright | Copyright © 2014 Dinan et al. 2014. This work is published under http://creativecommons.org/licenses/by/3.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. Copyright © 2014 Dinan et al. 2014 Dinan et al. |
| Copyright_xml | – notice: Copyright © 2014 Dinan et al. – notice: 2014. This work is published under http://creativecommons.org/licenses/by/3.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. – notice: Copyright © 2014 Dinan et al. 2014 Dinan et al. |
| DBID | AAYXX CITATION CGR CUY CVF ECM EIF NPM 8C1 8FE 8FH ABUWG AFKRA AZQEC BBNVY BENPR BHPHI CCPQU DWQXO FYUFA GHDGH GNUQQ HCIFZ LK8 M7P PHGZM PHGZT PIMPY PJZUB PKEHL PPXIY PQEST PQGLB PQQKQ PQUKI PRINS 7X8 7S9 L.6 5PM DOA |
| DOI | 10.1128/mBio.01169-14 |
| DatabaseName | CrossRef Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed Public Health Database ProQuest SciTech Collection ProQuest Natural Science Collection ProQuest Central (Alumni) ProQuest Central UK/Ireland ProQuest Central Essentials - QC Biological Science Collection ProQuest Central Natural Science Collection ProQuest One Community College ProQuest Central Korea ProQuest Health & Medical Collection Health Research Premium Collection (Alumni) ProQuest Central Student SciTech Premium Collection Biological Sciences Biological Science Database Proquest Central Premium ProQuest One Academic Publicly Available Content Database ProQuest Health & Medical Research Collection ProQuest One Academic Middle East (New) ProQuest One Health & Nursing ProQuest One Academic Eastern Edition (DO NOT USE) ProQuest One Applied & Life Sciences ProQuest One Academic (retired) ProQuest One Academic UKI Edition ProQuest Central China MEDLINE - Academic AGRICOLA AGRICOLA - Academic PubMed Central (Full Participant titles) Directory of Open Access Journals (DOAJ) |
| DatabaseTitle | CrossRef MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) Publicly Available Content Database ProQuest Central Student ProQuest One Academic Middle East (New) ProQuest Central Essentials ProQuest Central (Alumni Edition) SciTech Premium Collection ProQuest One Community College ProQuest One Health & Nursing ProQuest Natural Science Collection ProQuest Central China ProQuest Central ProQuest One Applied & Life Sciences ProQuest Health & Medical Research Collection Health Research Premium Collection Natural Science Collection ProQuest Central Korea Health & Medical Research Collection Biological Science Collection ProQuest Central (New) ProQuest Public Health ProQuest Biological Science Collection ProQuest One Academic Eastern Edition Health Research Premium Collection (Alumni) Biological Science Database ProQuest SciTech Collection ProQuest One Academic UKI Edition ProQuest One Academic ProQuest One Academic (New) MEDLINE - Academic AGRICOLA AGRICOLA - Academic |
| DatabaseTitleList | MEDLINE - Academic CrossRef Publicly Available Content Database AGRICOLA MEDLINE |
| Database_xml | – sequence: 1 dbid: DOA name: DOAJ Directory of Open Access Journals url: https://www.doaj.org/ sourceTypes: Open Website – sequence: 2 dbid: NPM name: PubMed url: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 3 dbid: PIMPY name: Publicly Available Content Database url: http://search.proquest.com/publiccontent sourceTypes: Aggregation Database |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Biology |
| DocumentTitleAlternate | Relaxed Selection Drives Noisy Noncoding Transcriptome |
| EISSN | 2150-7511 |
| ExternalDocumentID | oai_doaj_org_article_dfb4712138a24332a916eb22066a1dc4 PMC4128351 25096875 10_1128_mBio_01169_14 |
| Genre | Research Support, Non-U.S. Gov't Journal Article |
| GrantInformation_xml | – fundername: Wellcome Trust grantid: 099817/Z/12/Z – fundername: Wellcome Trust |
| GroupedDBID | --- 0R~ 53G 5VS 8C1 AAFWJ AAGFI AAUOK AAYXX ABUWG ADBBV ADRAZ AENEX AFFHD AFKRA AFPKN ALMA_UNASSIGNED_HOLDINGS AOIJS BAWUL BBNVY BCNDV BENPR BHPHI BTFSW CCPQU CITATION DIK E3Z EBS EJD FRP FYUFA GROUPED_DOAJ GX1 H13 HCIFZ HYE HZ~ KQ8 M48 M7P O5R O5S O9- OK1 P2P PGMZT PHGZM PHGZT PIMPY PJZUB PPXIY PQGLB RHI RNS RPM RSF UKHRP CGR CUY CVF ECM EIF NPM 8FE 8FH AZQEC DWQXO GNUQQ LK8 PKEHL PQEST PQQKQ PQUKI PRINS 7X8 7S9 L.6 5PM |
| ID | FETCH-LOGICAL-c584t-b70025411eb1edc727a7c8118d4367873c5809870742636a04ce942e20a051c83 |
| IEDL.DBID | M7P |
| ISICitedReferencesCount | 19 |
| ISICitedReferencesURI | http://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=Summon&SrcAuth=ProQuest&DestLinkType=CitingArticles&DestApp=WOS_CPL&KeyUT=000341588100023&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D |
| ISSN | 2161-2129 2150-7511 |
| IngestDate | Fri Oct 03 12:39:38 EDT 2025 Tue Nov 04 01:55:22 EST 2025 Sun Nov 09 12:49:49 EST 2025 Thu Jul 10 17:17:21 EDT 2025 Sat Oct 25 01:45:55 EDT 2025 Mon Jul 21 05:53:53 EDT 2025 Tue Nov 18 21:48:49 EST 2025 Sat Nov 29 03:10:43 EST 2025 |
| IsDoiOpenAccess | true |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 4 |
| Language | English |
| License | Copyright © 2014 Dinan et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 3.0 Unported license. |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-c584t-b70025411eb1edc727a7c8118d4367873c5809870742636a04ce942e20a051c83 |
| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 Editor Julian Parkhill, The Sanger Institute |
| OpenAccessLink | https://www.proquest.com/docview/3263479821?pq-origsite=%requestingapplication% |
| PMID | 25096875 |
| PQID | 3263479821 |
| PQPubID | 7421146 |
| ParticipantIDs | doaj_primary_oai_doaj_org_article_dfb4712138a24332a916eb22066a1dc4 pubmedcentral_primary_oai_pubmedcentral_nih_gov_4128351 proquest_miscellaneous_1746406347 proquest_miscellaneous_1551820852 proquest_journals_3263479821 pubmed_primary_25096875 crossref_citationtrail_10_1128_mBio_01169_14 crossref_primary_10_1128_mBio_01169_14 |
| PublicationCentury | 2000 |
| PublicationDate | 20140805 |
| PublicationDateYYYYMMDD | 2014-08-05 |
| PublicationDate_xml | – month: 8 year: 2014 text: 20140805 day: 5 |
| PublicationDecade | 2010 |
| PublicationPlace | United States |
| PublicationPlace_xml | – name: United States – name: Washington – name: 1752 N St., N.W., Washington, DC |
| PublicationTitle | mBio |
| PublicationTitleAlternate | mBio |
| PublicationYear | 2014 |
| Publisher | American Society for Microbiology American Society of Microbiology |
| Publisher_xml | – name: American Society for Microbiology – name: American Society of Microbiology |
| References | e_1_3_2_26_2 e_1_3_2_49_2 e_1_3_2_28_2 e_1_3_2_41_2 e_1_3_2_20_2 e_1_3_2_43_2 e_1_3_2_22_2 e_1_3_2_45_2 e_1_3_2_24_2 e_1_3_2_47_2 e_1_3_2_9_2 e_1_3_2_16_2 e_1_3_2_37_2 e_1_3_2_7_2 e_1_3_2_18_2 e_1_3_2_39_2 e_1_3_2_10_2 e_1_3_2_31_2 e_1_3_2_52_2 e_1_3_2_5_2 e_1_3_2_12_2 e_1_3_2_33_2 e_1_3_2_3_2 e_1_3_2_14_2 e_1_3_2_35_2 e_1_3_2_50_2 e_1_3_2_27_2 e_1_3_2_48_2 e_1_3_2_29_2 e_1_3_2_40_2 e_1_3_2_21_2 e_1_3_2_42_2 e_1_3_2_23_2 e_1_3_2_44_2 e_1_3_2_25_2 e_1_3_2_46_2 e_1_3_2_15_2 e_1_3_2_38_2 e_1_3_2_8_2 e_1_3_2_17_2 e_1_3_2_6_2 e_1_3_2_19_2 e_1_3_2_30_2 e_1_3_2_53_2 e_1_3_2_32_2 e_1_3_2_51_2 e_1_3_2_11_2 e_1_3_2_34_2 e_1_3_2_4_2 e_1_3_2_13_2 e_1_3_2_36_2 e_1_3_2_2_2 |
| References_xml | – ident: e_1_3_2_51_2 doi: 10.1186/1471-2105-10-154 – ident: e_1_3_2_37_2 doi: 10.1038/35059006 – ident: e_1_3_2_14_2 doi: 10.1046/j.1365-2958.1997.6361999.x – ident: e_1_3_2_31_2 doi: 10.1074/jbc.M111.302588 – ident: e_1_3_2_33_2 doi: 10.1128/IAI.01495-08 – ident: e_1_3_2_34_2 doi: 10.1093/nar/gkq454 – ident: e_1_3_2_53_2 doi: 10.1101/gr.092759.109 – ident: e_1_3_2_6_2 doi: 10.1038/nrmicro1472 – ident: e_1_3_2_22_2 doi: 10.1371/journal.ppat.1002342 – ident: e_1_3_2_41_2 doi: 10.1371/journal.pone.0074209 – ident: e_1_3_2_19_2 doi: 10.1128/JB.05947-11 – ident: e_1_3_2_9_2 doi: 10.1186/gb-2011-12-10-r98 – ident: e_1_3_2_38_2 doi: 10.1261/rna.034116.112 – ident: e_1_3_2_44_2 doi: 10.1186/1471-2164-14-710 – ident: e_1_3_2_8_2 doi: 10.1038/nature08756 – ident: e_1_3_2_12_2 doi: 10.1371/journal.pgen.1002867 – ident: e_1_3_2_7_2 doi: 10.1146/annurev.micro.62.081307.162832 – ident: e_1_3_2_10_2 doi: 10.1128/mBio.00156-12 – ident: e_1_3_2_32_2 doi: 10.1128/AAC.02191-12 – ident: e_1_3_2_4_2 doi: 10.1101/gr.075069.107 – ident: e_1_3_2_26_2 doi: 10.1073/pnas.0505446102 – ident: e_1_3_2_42_2 doi: 10.1371/journal.pone.0079411 – ident: e_1_3_2_28_2 doi: 10.1016/j.molcel.2013.10.013 – ident: e_1_3_2_24_2 doi: 10.1371/journal.pone.0051950 – ident: e_1_3_2_5_2 doi: 10.1016/j.tim.2010.12.008 – ident: e_1_3_2_13_2 doi: 10.1016/j.tube.2012.11.012 – ident: e_1_3_2_18_2 doi: 10.1016/j.celrep.2013.10.031 – ident: e_1_3_2_27_2 doi: 10.1111/j.1365-2958.2008.06576.x – ident: e_1_3_2_49_2 doi: 10.1073/pnas.1315974111 – ident: e_1_3_2_3_2 doi: 10.1073/pnas.1130426100 – ident: e_1_3_2_46_2 doi: 10.1099/mic.0.2007/009894-0 – ident: e_1_3_2_15_2 doi: 10.1186/1471-2164-14-145 – ident: e_1_3_2_43_2 doi: 10.1093/gbe/evt138 – ident: e_1_3_2_40_2 doi: 10.1093/nar/gkq101 – ident: e_1_3_2_11_2 doi: 10.1371/journal.pbio.0060311 – ident: e_1_3_2_35_2 doi: 10.1371/journal.pgen.1001115 – ident: e_1_3_2_23_2 doi: 10.1038/nmeth.1226 – ident: e_1_3_2_21_2 doi: 10.1186/gb-2010-11-9-r97 – ident: e_1_3_2_50_2 doi: 10.1186/gb-2009-10-3-r25 – ident: e_1_3_2_16_2 doi: 10.1016/j.tube.2010.08.003 – ident: e_1_3_2_36_2 doi: 10.1101/gr.129544.111 – ident: e_1_3_2_29_2 doi: 10.1016/j.molcel.2008.02.026 – ident: e_1_3_2_25_2 doi: 10.1074/jbc.M113.516385 – ident: e_1_3_2_45_2 doi: 10.1016/j.tube.2007.02.004 – ident: e_1_3_2_17_2 doi: 10.1016/j.ijmm.2013.09.010 – ident: e_1_3_2_20_2 doi: 10.1038/nmeth.2646 – ident: e_1_3_2_48_2 doi: 10.1371/journal.pone.0000745 – ident: e_1_3_2_2_2 doi: 10.1186/1471-2164-13-120 – ident: e_1_3_2_39_2 doi: 10.1016/j.molcel.2011.08.022 – ident: e_1_3_2_47_2 doi: 10.1126/science.1089370 – ident: e_1_3_2_30_2 doi: 10.1038/ncomms4501 – ident: e_1_3_2_52_2 doi: 10.1093/bioinformatics/btm404 |
| SSID | ssj0000331830 |
| Score | 2.1441925 |
| Snippet | Related species are often used to understand the molecular underpinning of virulence through examination of a shared set of biological features attributable to... ABSTRACT Related species are often used to understand the molecular underpinning of virulence through examination of a shared set of biological features... |
| SourceID | doaj pubmedcentral proquest pubmed crossref |
| SourceType | Open Website Open Access Repository Aggregation Database Index Database Enrichment Source |
| StartPage | e01169 |
| SubjectTerms | Evolution Evolution, Molecular Genes Genome, Bacterial - genetics Genomes Genomics Host preferences Multidrug resistance multiple drug resistance Mycobacterium marinum Mycobacterium tuberculosis Mycobacterium tuberculosis - genetics non-coding RNA Pathogenicity Pathogens Polymorphism, Single Nucleotide - genetics promoter regions Promoter Regions, Genetic - genetics Single-nucleotide polymorphism Species Transcription transcription (genetics) transcription factors transcriptome Transcriptome - genetics Transcriptomes Tuberculosis Virulence |
| SummonAdditionalLinks | – databaseName: Directory of Open Access Journals (DOAJ) dbid: DOA link: http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV1La9wwEBYltNBLadKXm6SoUHqqG0vWruRjkyb0kqXQFnITsjSmhl07rNcl--87I3uX3dLHpReDrcHoMY9vkPQNY2-kAZCTSqUB4pUc8GkRpiEtSwynRVk6AB-LTejZzNzcFJ93Sn3RmbCBHniYuLNQleg_pciNk8S15RDPYDZILOROBB-ZQBH17CRT0QfnpKvZhlRTmrPFed2-p12HIhVqLwhFrv7fAcxfz0nuBJ6rx-zRiBj5h6Gnh-weNEfswVBDcv2ELeg42x0E_iVWtMFp5h-XxCXLHZ-1dbfGZ-NbClE8BqboJtoF8Lrh10D1QDreVhyBIL9eezTvSN_cL_iqxzbfz9uu7jj5jTncPWXfri6_XnxKxxoKqUdosUpLHe-7C4E-GQeCaMVpbzCrCCrHOKVzFMsKNFpNzO1TlykPhZIgM4fm6k3-jB00bQMvGNd5qCowlcpyUCIrnQbIVND4XgaYZAl7t5lU60eCcapzMbcx0ZDG0hrYuAaYcSTs7Vb8dmDW-JPgOa3QVogIseMHVBM7qon9l5ok7GSzvna00s4idKWLtEaKhL3eNqN90aaJa6DtOxsp66iQqfyLjFZTBEb4r4Q9H1Rm21tJ_DqYFCZM7ynT3nD2W5r6e-T5VoLI8MTL_zH-Y_YQoZ6KRxcnJ-xgtezhlN33P1Z1t3wVjecnKYkfpw priority: 102 providerName: Directory of Open Access Journals |
| Title | Relaxed Selection Drives a Noisy Noncoding Transcriptome in Members of the Mycobacterium tuberculosis Complex |
| URI | https://www.ncbi.nlm.nih.gov/pubmed/25096875 https://www.proquest.com/docview/3263479821 https://www.proquest.com/docview/1551820852 https://www.proquest.com/docview/1746406347 https://pubmed.ncbi.nlm.nih.gov/PMC4128351 https://doaj.org/article/dfb4712138a24332a916eb22066a1dc4 |
| Volume | 5 |
| WOSCitedRecordID | wos000341588100023&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| journalDatabaseRights | – providerCode: PRVAON databaseName: DOAJ Directory of Open Access Journals customDbUrl: eissn: 2150-7511 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0000331830 issn: 2161-2129 databaseCode: DOA dateStart: 20100101 isFulltext: true titleUrlDefault: https://www.doaj.org/ providerName: Directory of Open Access Journals – providerCode: PRVPQU databaseName: Biological Science Database (ProQuest) customDbUrl: eissn: 2150-7511 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0000331830 issn: 2161-2129 databaseCode: M7P dateStart: 20100501 isFulltext: true titleUrlDefault: http://search.proquest.com/biologicalscijournals providerName: ProQuest – providerCode: PRVPQU databaseName: ProQuest Central customDbUrl: eissn: 2150-7511 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0000331830 issn: 2161-2129 databaseCode: BENPR dateStart: 20100501 isFulltext: true titleUrlDefault: https://www.proquest.com/central providerName: ProQuest – providerCode: PRVPQU databaseName: Public Health Database customDbUrl: eissn: 2150-7511 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0000331830 issn: 2161-2129 databaseCode: 8C1 dateStart: 20100501 isFulltext: true titleUrlDefault: https://search.proquest.com/publichealth providerName: ProQuest – providerCode: PRVPQU databaseName: Publicly Available Content Database customDbUrl: eissn: 2150-7511 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0000331830 issn: 2161-2129 databaseCode: PIMPY dateStart: 20100501 isFulltext: true titleUrlDefault: http://search.proquest.com/publiccontent providerName: ProQuest |
| link | http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV3db9MwELfYBhIvfA8CozIS4omwxHFr5wmxsQkeWkV8SOUpSuwLRGqS0TRo_e-5c9NAEeyFl0iJT1Gcs-9-Pp9_x9hzoQHEuJC-BXckB4wf24n18xzdaZznGYBxxSbUbKbn8zjpA25tn1a5tYnOUNvGUIz8GGEGHXrUInx98d2nqlG0u9qX0NhjB8SSELnUvWSIsQQRjVgKswgENj5a6XhLsyn0cZWXzSvah4j9UO64Jcfe_zfI-Wfm5G-u6Pz2_3biDrvVg1D-ZjNq7rJrUN9jNzZlKdf3WUUZcpdg-UdXJAc1x98uiZ6WZ3zWlO0ar7VpyOtx5-uc5Wkq4GXNp0AlRlreFByxJZ-uDVoMxwjdVXzVYZvpFk1btpxM0QIuH7DP52efTt_5fVkG3yBaWfm5ckfowxDNPP4JBECZMhoXKlZG6PpUhGJBjHZAERn8JAukgVgKEEGGFsDo6JDt100NjxhXkS0K0IUMIpBhkGcKIJBW4X1uYRx47OVWK6npOcupdMYidWsXodMK_03qlIiLGI-9GMQvNmQd_xI8IRUPQsSx7R40y69pP2VTW-TouUUY6UwQy1uGSBpyQfz3WWgNvuRoq-S0n_ht-kvDHns2NOOUpX2YrIama1PHgke1UcUVMkpOEGvhuzz2cDPmhq8VRNmD60yPqZ3RuNOd3Za6_Oaow2VI_Hrh46s__Qm7ibhQujzH8RHbXy07eMqumx-rsl2O2J6aa7zq03DEDk7OZsmHkQtljNzsw2fJ-2ny5SfYrjaL |
| linkProvider | ProQuest |
| linkToHtml | http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMw1V1Lb9NAEB6VFAQX3o9AgUUCTpja601sHxACStWoTRSJIrUnY--OwVJslziB5k_xG5nZ2IEg6K0HLpbiHa1s59tvZvbxDcBTGSLKXqYcg_ZIDmonMn3jpCm50yhNE0Rti00Eo1F4dBSNN-BHexaGt1W2nGiJ2lSa58i3KczgQ4-h9F6ffHW4ahSvrrYlNJaw2MfFd0rZ6leDHfp_n0m5-_7w3Z7TVBVwNDnbmZMG9gS45xFLodHkv5NAhxRnG-UTcwc-mbmUiXPS2Pf7ias0RkqidBMCsA596vcCbCoCe9iBzfFgOD5ezeq4Po8RntiRFEo55BeiVthThttFmlcveeUjcjy15ghtvYC_Bbl_7tX8zfntXvvfPtt1uNqE2eLNclzcgA0sb8KlZeHNxS0oeA_gKRrxwZYBImyKnSkL8IpEjKq8XtC11BX7dWG9ueXWqkCRl2KIXESlFlUmKHoWw4UmTrSa1_NCzObUpueTqs5rwWQ7wdPb8PFc3vUOdMqqxHsgAt9kGYaZcn1UnpsmAaKrTEC_U4M9twsvWhTEulFl5-Igk9hmZzKMC_o2sQUNpWldeL4yP1nKkfzL8C1DamXEKuL2RjX9HDekFJsspdhEen6YSNaxSyhXwFSywn_iGU2dbLWgihtqq-NfiOrCk1UzkRKvNCUlVvM6tjp_XP1VnmETqD5Fk9RXF-4uMb56WsmiRJRJdyFYQ__a66y3lPkXK46uPFYQ9O6f_eiP4fLe4fAgPhiM9h_AFYqCld3V2duCzmw6x4dwUX-b5fX0UTPCBXw679HxEyZKiiE |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Relaxed+Selection+Drives+a+Noisy+Noncoding+Transcriptome+in+Members+of+the+Mycobacterium+tuberculosis+Complex&rft.jtitle=mBio&rft.au=Dinan%2C+Adam+M.&rft.au=Tong%2C+Pin&rft.au=Lohan%2C+Amanda+J.&rft.au=Conlon%2C+Kevin+M.&rft.date=2014-08-05&rft.issn=2161-2129&rft.eissn=2150-7511&rft.volume=5&rft.issue=4&rft_id=info:doi/10.1128%2FmBio.01169-14&rft.externalDBID=n%2Fa&rft.externalDocID=10_1128_mBio_01169_14 |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=2161-2129&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=2161-2129&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=2161-2129&client=summon |