The Long Non-Coding Antisense RNA JHDM1D-AS1 Regulates Inflammatory Responses in Human Monocytes

Monocytes are key players in innate immunity, with their ability to regulate inflammatory responses and combat invading pathogens. There is a growing body of evidence indicating that long non-coding RNA (lncRNA) participate in various cellular biological processes, including the innate immune respon...

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Vydáno v:	Frontiers in cellular and infection microbiology Ročník 12; s. 934313
Hlavní autoři:	Malmström, Erik, Khan, Hina N., Veer, Cornelis van ‘t, Stunnenberg, Melissa, Meijer, Mariska T., Matsumoto, Hisatake, Otto, Natasja A., Geijtenbeek, Teunis B. H., de Vos, Alex F., Poll, Tom van der, Scicluna, Brendon P.
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	Switzerland Frontiers Media SA 12.07.2022 Frontiers Media S.A
Témata:	Antisense RNA Basic Medicine Cell and Molecular Biology Cell- och molekylärbiologi Cells Cellular and Infection Microbiology Cytokines Down-regulation Enzymes Gene expression Humans Immune response Immunoregulation Inflammation Innate immunity Lipopolysaccharides Lipopolysaccharides - metabolism long non-coding RNA Macrophages Macrophages - metabolism Medical and Health Sciences Medical research Medicin och hälsovetenskap Medicinska och farmaceutiska grundvetenskaper MicroRNAs monocyte Monocytes Non-coding RNA Pathogens RNA, Antisense - metabolism RNA, Long Noncoding - metabolism Sepsis Toll-like receptors Tumor necrosis factor-TNF Amarillo Texas United Kingdom > UK United States > US Germany monocyte long non-coding RNA toll-like receptors inflammation sepsis
ISSN:	2235-2988, 2235-2988
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Shrnutí:	Monocytes are key players in innate immunity, with their ability to regulate inflammatory responses and combat invading pathogens. There is a growing body of evidence indicating that long non-coding RNA (lncRNA) participate in various cellular biological processes, including the innate immune response. The immunoregulatory properties of numerous lncRNAs discovered in monocytes remain largely unexplored. Here, by RNA sequencing, we identified a lncRNA JHDM1D-AS1, which was upregulated in blood monocytes obtained from patients with sepsis relative to healthy controls. JHDM1D-AS1 expression was induced in primary human monocytes exposed to Toll-like receptor ligands, such as lipopolysaccharide (LPS), or bacteria. The inducibility of JHDM1D-AS1 expression in monocytes depended, at least in part, on nuclear factor–κB activation. JHDM1D-AS1 knockdown experiments in human monocyte-derived macrophages revealed significantly enhanced expression of inflammatory mediators, before and after exposure to LPS, relative to control cells. Specifically, genes involved in inflammatory responses were upregulated (e.g., CXCL2 , CXCL8 , IL1RN , TREM1 , TNF , and IL6 ), whereas genes involved in anti-inflammatory pathways were downregulated (e.g., SOCS1 and IL10RA ). JHDM1D-AS1 overexpression in a pro-monocytic cell line revealed diminished pro-inflammatory responses subsequent to LPS challenge. Collectively, these findings identify JHDM1D-AS1 as a potential anti-inflammatory mediator induced in response to inflammatory stimuli.
Bibliografie:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 This article was submitted to Clinical Microbiology, a section of the journal Frontiers in Cellular and Infection Microbiology Reviewed by: Sarantis Korniotis, Oncologie (INSERM), France; Justyna Agier, Medical University of Lodz, Poland Edited by: Tie-Ning Zhang, ShengJing Hospital of China Medical University, China
ISSN:	2235-2988 2235-2988
DOI:	10.3389/fcimb.2022.934313