Defining quantification methods and optimizing protocols for microarray hybridization of circulating microRNAs

MicroRNAs (miRNAs) have emerged as promising biomarkers of disease. Their potential use in clinical practice requires standardized protocols with very low miRNA concentrations, particularly in plasma samples. Here we tested the most appropriate method for miRNA quantification and validated the perfo...

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Bibliographic Details
Published in:	Scientific reports Vol. 7; no. 1; pp. 7725 - 14
Main Authors:	Garcia-Elias, Anna, Alloza, Leonor, Puigdecanet, Eulàlia, Nonell, Lara, Tajes, Marta, Curado, Joao, Enjuanes, Cristina, Díaz, Oscar, Bruguera, Jordi, Martí-Almor, Julio, Comín-Colet, Josep, Benito, Begoña
Format:	Journal Article
Language:	English
Published:	London Nature Publishing Group UK 10.08.2017 Nature Publishing Group Nature Portfolio
Subjects:	38/39 38/47 38/61 38/90 45 631/1647/2217/748 692/53/2423 Biochemical markers Biomarkers Blood plasma Circulating MicroRNA Computational Biology - methods Contaminants Expressió gènica Gene expression Gene Expression Profiling - instrumentation Gene Expression Profiling - methods Humanities and Social Sciences Humans Hybridization Marcadors bioquímics Medical protocols Micro RNAs MicroRNAs miRNA multidisciplinary Nucleic Acid Hybridization - methods Plasma Plasma sanguini Protocols clínics Reproducibility of Results Science Science (multidisciplinary) Spectrophotometers Transcriptome
ISSN:	2045-2322, 2045-2322
Online Access:	Get full text
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