Defining quantification methods and optimizing protocols for microarray hybridization of circulating microRNAs

MicroRNAs (miRNAs) have emerged as promising biomarkers of disease. Their potential use in clinical practice requires standardized protocols with very low miRNA concentrations, particularly in plasma samples. Here we tested the most appropriate method for miRNA quantification and validated the perfo...

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Vydané v:	Scientific reports Ročník 7; číslo 1; s. 7725 - 14
Hlavní autori:	Garcia-Elias, Anna, Alloza, Leonor, Puigdecanet, Eulàlia, Nonell, Lara, Tajes, Marta, Curado, Joao, Enjuanes, Cristina, Díaz, Oscar, Bruguera, Jordi, Martí-Almor, Julio, Comín-Colet, Josep, Benito, Begoña
Médium:	Journal Article
Jazyk:	English
Vydavateľské údaje:	London Nature Publishing Group UK 10.08.2017 Nature Publishing Group Nature Portfolio
Predmet:	38/39 38/47 38/61 38/90 45 631/1647/2217/748 692/53/2423 Biochemical markers Biomarkers Blood plasma Circulating MicroRNA Computational Biology - methods Contaminants Expressió gènica Gene expression Gene Expression Profiling - instrumentation Gene Expression Profiling - methods Humanities and Social Sciences Humans Hybridization Marcadors bioquímics Medical protocols Micro RNAs MicroRNAs miRNA multidisciplinary Nucleic Acid Hybridization - methods Plasma Plasma sanguini Protocols clínics Reproducibility of Results Science Science (multidisciplinary) Spectrophotometers Transcriptome
ISSN:	2045-2322, 2045-2322
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Abstract	MicroRNAs (miRNAs) have emerged as promising biomarkers of disease. Their potential use in clinical practice requires standardized protocols with very low miRNA concentrations, particularly in plasma samples. Here we tested the most appropriate method for miRNA quantification and validated the performance of a hybridization platform using lower amounts of starting RNA. miRNAs isolated from human plasma and from a reference sample were quantified using four platforms and profiled with hybridization arrays and RNA sequencing (RNA-seq). Our results indicate that the Infinite ® 200 PRO Nanoquant and Nanodrop 2000 spectrophotometers magnified the miRNA concentration by detecting contaminants, proteins, and other forms of RNA. The Agilent 2100 Bioanalyzer PicoChip and SmallChip gave valuable information on RNA profile but were not a reliable quantification method for plasma samples. The Qubit ® 2.0 Fluorometer provided the most accurate quantification of miRNA content, although RNA-seq confirmed that only ~58% of small RNAs in plasma are true miRNAs. On the other hand, reducing the starting RNA to 70% of the recommended amount for miRNA profiling with arrays yielded results comparable to those obtained with the full amount, whereas a 50% reduction did not. These findings provide important clues for miRNA determination in human plasma samples.
AbstractList	MicroRNAs (miRNAs) have emerged as promising biomarkers of disease. Their potential use in clinical practice requires standardized protocols with very low miRNA concentrations, particularly in plasma samples. Here we tested the most appropriate method for miRNA quantification and validated the performance of a hybridization platform using lower amounts of starting RNA. miRNAs isolated from human plasma and from a reference sample were quantified using four platforms and profiled with hybridization arrays and RNA sequencing (RNA-seq). Our results indicate that the Infinite ® 200 PRO Nanoquant and Nanodrop 2000 spectrophotometers magnified the miRNA concentration by detecting contaminants, proteins, and other forms of RNA. The Agilent 2100 Bioanalyzer PicoChip and SmallChip gave valuable information on RNA profile but were not a reliable quantification method for plasma samples. The Qubit ® 2.0 Fluorometer provided the most accurate quantification of miRNA content, although RNA-seq confirmed that only ~58% of small RNAs in plasma are true miRNAs. On the other hand, reducing the starting RNA to 70% of the recommended amount for miRNA profiling with arrays yielded results comparable to those obtained with the full amount, whereas a 50% reduction did not. These findings provide important clues for miRNA determination in human plasma samples. Abstract MicroRNAs (miRNAs) have emerged as promising biomarkers of disease. Their potential use in clinical practice requires standardized protocols with very low miRNA concentrations, particularly in plasma samples. Here we tested the most appropriate method for miRNA quantification and validated the performance of a hybridization platform using lower amounts of starting RNA. miRNAs isolated from human plasma and from a reference sample were quantified using four platforms and profiled with hybridization arrays and RNA sequencing (RNA-seq). Our results indicate that the Infinite® 200 PRO Nanoquant and Nanodrop 2000 spectrophotometers magnified the miRNA concentration by detecting contaminants, proteins, and other forms of RNA. The Agilent 2100 Bioanalyzer PicoChip and SmallChip gave valuable information on RNA profile but were not a reliable quantification method for plasma samples. The Qubit® 2.0 Fluorometer provided the most accurate quantification of miRNA content, although RNA-seq confirmed that only ~58% of small RNAs in plasma are true miRNAs. On the other hand, reducing the starting RNA to 70% of the recommended amount for miRNA profiling with arrays yielded results comparable to those obtained with the full amount, whereas a 50% reduction did not. These findings provide important clues for miRNA determination in human plasma samples. MicroRNAs (miRNAs) have emerged as promising biomarkers of disease. Their potential use in clinical practice requires standardized protocols with very low miRNA concentrations, particularly in plasma samples. Here we tested the most appropriate method for miRNA quantification and validated the performance of a hybridization platform using lower amounts of starting RNA. miRNAs isolated from human plasma and from a reference sample were quantified using four platforms and profiled with hybridization arrays and RNA sequencing (RNA-seq). Our results indicate that the Infinite® 200 PRO Nanoquant and Nanodrop 2000 spectrophotometers magnified the miRNA concentration by detecting contaminants, proteins, and other forms of RNA. The Agilent 2100 Bioanalyzer PicoChip and SmallChip gave valuable information on RNA profile but were not a reliable quantification method for plasma samples. The Qubit® 2.0 Fluorometer provided the most accurate quantification of miRNA content, although RNA-seq confirmed that only ~58% of small RNAs in plasma are true miRNAs. On the other hand, reducing the starting RNA to 70% of the recommended amount for miRNA profiling with arrays yielded results comparable to those obtained with the full amount, whereas a 50% reduction did not. These findings provide important clues for miRNA determination in human plasma samples. MicroRNAs (miRNAs) have emerged as promising biomarkers of disease. Their potential use in clinical practice requires standardized protocols with very low miRNA concentrations, particularly in plasma samples. Here we tested the most appropriate method for miRNA quantification and validated the performance of a hybridization platform using lower amounts of starting RNA. miRNAs isolated from human plasma and from a reference sample were quantified using four platforms and profiled with hybridization arrays and RNA sequencing (RNA-seq). Our results indicate that the Infinite® 200 PRO Nanoquant and Nanodrop 2000 spectrophotometers magnified the miRNA concentration by detecting contaminants, proteins, and other forms of RNA. The Agilent 2100 Bioanalyzer PicoChip and SmallChip gave valuable information on RNA profile but were not a reliable quantification method for plasma samples. The Qubit® 2.0 Fluorometer provided the most accurate quantification of miRNA content, although RNA-seq confirmed that only ~58% of small RNAs in plasma are true miRNAs. On the other hand, reducing the starting RNA to 70% of the recommended amount for miRNA profiling with arrays yielded results comparable to those obtained with the full amount, whereas a 50% reduction did not. These findings provide important clues for miRNA determination in human plasma samples.MicroRNAs (miRNAs) have emerged as promising biomarkers of disease. Their potential use in clinical practice requires standardized protocols with very low miRNA concentrations, particularly in plasma samples. Here we tested the most appropriate method for miRNA quantification and validated the performance of a hybridization platform using lower amounts of starting RNA. miRNAs isolated from human plasma and from a reference sample were quantified using four platforms and profiled with hybridization arrays and RNA sequencing (RNA-seq). Our results indicate that the Infinite® 200 PRO Nanoquant and Nanodrop 2000 spectrophotometers magnified the miRNA concentration by detecting contaminants, proteins, and other forms of RNA. The Agilent 2100 Bioanalyzer PicoChip and SmallChip gave valuable information on RNA profile but were not a reliable quantification method for plasma samples. The Qubit® 2.0 Fluorometer provided the most accurate quantification of miRNA content, although RNA-seq confirmed that only ~58% of small RNAs in plasma are true miRNAs. On the other hand, reducing the starting RNA to 70% of the recommended amount for miRNA profiling with arrays yielded results comparable to those obtained with the full amount, whereas a 50% reduction did not. These findings provide important clues for miRNA determination in human plasma samples. MicroRNAs (miRNAs) have emerged as promising biomarkers of disease. Their potential use in clinical practice requires standardized protocols with very low miRNA concentrations, particularly in plasma samples. Here we tested the most appropriate method for miRNA quantification and validated the performance of a hybridization platform using lower amounts of starting RNA. miRNAs isolated from human plasma and from a reference sample were quantified using four platforms and profiled with hybridization arrays and RNA sequencing (RNA-seq). Our results indicate that the Infinite 200 PRO Nanoquant and Nanodrop 2000 spectrophotometers magnified the miRNA concentration by detecting contaminants, proteins, and other forms of RNA. The Agilent 2100 Bioanalyzer PicoChip and SmallChip gave valuable information on RNA profile but were not a reliable quantification method for plasma samples. The Qubit 2.0 Fluorometer provided the most accurate quantification of miRNA content, although RNA-seq confirmed that only ~58% of small RNAs in plasma are true miRNAs. On the other hand, reducing the starting RNA to 70% of the recommended amount for miRNA profiling with arrays yielded results comparable to those obtained with the full amount, whereas a 50% reduction did not. These findings provide important clues for miRNA determination in human plasma samples.
ArticleNumber	7725
Author	Puigdecanet, Eulàlia Bruguera, Jordi Nonell, Lara Tajes, Marta Comín-Colet, Josep Curado, Joao Benito, Begoña Alloza, Leonor Enjuanes, Cristina Martí-Almor, Julio Díaz, Oscar Garcia-Elias, Anna
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BackLink	https://www.ncbi.nlm.nih.gov/pubmed/28798363$$D View this record in MEDLINE/PubMed
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Cites_doi	10.1016/j.ab.2012.09.007 10.1016/j.ab.2014.08.006 10.1146/annurev-biochem-060308-103103 10.1016/j.ymeth.2010.01.032 10.1016/j.cell.2009.01.002 10.1186/1471-2164-12-435 10.1186/1471-2164-15-395 10.1016/j.cell.2004.12.035 10.1073/pnas.0804549105 10.1161/CIRCRESAHA.111.247452 10.1016/j.ymeth.2012.09.015 10.1016/j.addr.2014.09.001 10.1371/journal.pone.0020769 10.1038/nrclinonc.2011.76 10.1038/nrg3198 10.1371/journal.pone.0082753 10.3390/molecules19021568 10.1038/nmeth.3014 10.1371/journal.pone.0041561 10.1261/rna.036863.112 10.1186/s12867-015-0039-3 10.1038/srep19529 10.1111/jcmm.12236 10.1371/journal.pone.0064795 10.1097/PDM.0b013e318269de3b 10.1373/clinchem.2009.141580 10.1007/s00216-015-8846-4 10.1373/clinchem.2014.221341 10.1007/s11684-014-0379-2
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References	Jensen (CR14) 2011; 12 Wang (CR21) 2012; 7 CR19 CR17 Ge (CR29) 2014; 19 Jung (CR13) 2010; 56 Cortez (CR8) 2011; 8 Cheng (CR16) 2015; 81C Deben (CR24) 2013; 22 Lewis, Burge, Bartel (CR3) 2005; 120 Moret (CR22) 2013; 8 Monleau (CR20) 2014; 15 Duttagupta, Jiang, Gollub, Getts, Jones (CR12) 2011; 6 Li, Bn-Dov, Mauro, Williams (CR27) 2015; 16 Bartel (CR1) 2009; 136 Pritchard, Cheng, Tewari (CR25) 2012; 13 Lee, Cheung, Leung (CR26) 2014; 465 Blondal (CR4) 2013; 59 Moldovan (CR6) 2014; 18 CR28 Creemers, Tijsen, Pinto (CR10) 2012; 110 Burgos (CR9) 2013; 19 El-Khoury, Pierson, Kaoma, Bernardin, Berchem (CR23) 2016; 6 Fabian, Sonenberg, Filipowicz (CR2) 2010; 79 Mitchell (CR11) 2008; 105 Kroh, Parkin, Mitchell, Tewari (CR7) 2010; 50 Cheng (CR18) 2013; 8 Mestdagh (CR15) 2014; 11 Li, Kowdley (CR5) 2012; 431 KL Burgos (8134_CR9) 2013; 19 PS Mitchell (8134_CR11) 2008; 105 K Wang (8134_CR21) 2012; 7 V El-Khoury (8134_CR23) 2016; 6 CC Pritchard (8134_CR25) 2012; 13 BP Lewis (8134_CR3) 2005; 120 M Jung (8134_CR13) 2010; 56 I Moret (8134_CR22) 2013; 8 R Duttagupta (8134_CR12) 2011; 6 P Mestdagh (8134_CR15) 2014; 11 M Monleau (8134_CR20) 2014; 15 8134_CR19 G Cheng (8134_CR16) 2015; 81C 8134_CR17 DP Bartel (8134_CR1) 2009; 136 X Li (8134_CR27) 2015; 16 MR Fabian (8134_CR2) 2010; 79 EE Creemers (8134_CR10) 2012; 110 Q Ge (8134_CR29) 2014; 19 L Moldovan (8134_CR6) 2014; 18 JTY Lee (8134_CR26) 2014; 465 SG Jensen (8134_CR14) 2011; 12 T Blondal (8134_CR4) 2013; 59 MA Cortez (8134_CR8) 2011; 8 Y Li (8134_CR5) 2012; 431 C Deben (8134_CR24) 2013; 22 HH Cheng (8134_CR18) 2013; 8 8134_CR28 EM Kroh (8134_CR7) 2010; 50
References_xml	– volume: 431 start-page: 69 year: 2012 end-page: 75 ident: CR5 article-title: Method for microRNA isolation from clinical serum samples publication-title: Anal. Biochem. doi: 10.1016/j.ab.2012.09.007 – volume: 465 start-page: 179 year: 2014 end-page: 86 ident: CR26 article-title: Correction for concentration overestimation of nucleic acids with phenol publication-title: Anal. Biochem. doi: 10.1016/j.ab.2014.08.006 – volume: 79 start-page: 351 year: 2010 end-page: 79 ident: CR2 article-title: Regulation of mRNA translation and stability by microRNAs publication-title: Annu. Rev. Biochem. doi: 10.1146/annurev-biochem-060308-103103 – volume: 50 start-page: 298 year: 2010 end-page: 301 ident: CR7 article-title: Analysis of circulating microRNA biomarkers in plasma and serum using quantitative reverse transcription-PCR (qRT-PCR) publication-title: Methods doi: 10.1016/j.ymeth.2010.01.032 – volume: 136 start-page: 215 year: 2009 end-page: 33 ident: CR1 article-title: MicroRNAs: target recognition and regulatory functions publication-title: Cell doi: 10.1016/j.cell.2009.01.002 – volume: 12 start-page: 435 year: 2011 ident: CR14 article-title: Evaluation of two commercial global miRNA expression profiling platforms for detection of less abundant miRNAs publication-title: BMC Genomics doi: 10.1186/1471-2164-12-435 – volume: 15 start-page: 395 year: 2014 ident: CR20 article-title: Comparison of different extraction techniques to profile microRNAs from human sera and peripheral blood mononuclear cells publication-title: BMC Genomics doi: 10.1186/1471-2164-15-395 – volume: 120 start-page: 15 year: 2005 end-page: 20 ident: CR3 article-title: Conserved seed pairing, often flanked by adenosines, indicates that thousands of human genes are microRNA targets publication-title: Cell doi: 10.1016/j.cell.2004.12.035 – volume: 105 start-page: 10513 year: 2008 end-page: 8 ident: CR11 article-title: Circulating microRNAs as stable blood-based markers for cancer detection publication-title: Proc. Natl. Acad. Sci. U. S. A. doi: 10.1073/pnas.0804549105 – volume: 110 start-page: 483 year: 2012 end-page: 95 ident: CR10 article-title: Circulating microRNAs: novel biomarkers and extracellular communicators in cardiovascular disease? publication-title: Circ. Res. doi: 10.1161/CIRCRESAHA.111.247452 – volume: 59 start-page: 164 year: 2013 end-page: 169 ident: CR4 article-title: Assessing sample and miRNA profile quality in serum and plasma or other biofluids publication-title: Methods doi: 10.1016/j.ymeth.2012.09.015 – volume: 81C start-page: 75 year: 2015 end-page: 93 ident: CR16 article-title: Circulating miRNAs: Roles in cancer diagnosis, prognosis and therapy publication-title: Adv. Drug Deliv. Rev. doi: 10.1016/j.addr.2014.09.001 – volume: 6 start-page: e20769 year: 2011 ident: CR12 article-title: Impact of cellular miRNAs on circulating miRNA biomarker signatures publication-title: PLoS One doi: 10.1371/journal.pone.0020769 – volume: 8 start-page: 467 year: 2011 end-page: 77 ident: CR8 article-title: MicroRNAs in body fluids–the mix of hormones and biomarkers publication-title: Nat. Rev. Clin. Oncol. doi: 10.1038/nrclinonc.2011.76 – volume: 13 start-page: 358 year: 2012 end-page: 369 ident: CR25 article-title: MicroRNA profiling: approaches and considerations publication-title: Nat. Rev. Genet. doi: 10.1038/nrg3198 – ident: CR19 – volume: 8 start-page: e82753 year: 2013 ident: CR22 article-title: Assessing an improved protocol for plasma microRNA extraction publication-title: PLoS One doi: 10.1371/journal.pone.0082753 – volume: 19 start-page: 1568 year: 2014 end-page: 1575 ident: CR29 article-title: MiRNA in plasma exosome is stable under different storage conditions publication-title: Molecules doi: 10.3390/molecules19021568 – ident: CR17 – volume: 11 start-page: 809 year: 2014 end-page: 815 ident: CR15 article-title: Evaluation of quantitative miRNA expression platforms in the microRNA quality control (miRQC) study publication-title: Nat. Methods doi: 10.1038/nmeth.3014 – volume: 7 start-page: e41561 year: 2012 ident: CR21 article-title: Comparing the MicroRNA spectrum between serum and plasma publication-title: PLoS One doi: 10.1371/journal.pone.0041561 – volume: 19 start-page: 712 year: 2013 end-page: 22 ident: CR9 article-title: Identification of extracellular miRNA in human cerebrospinal fluid by next-generation sequencing publication-title: RNA doi: 10.1261/rna.036863.112 – volume: 16 start-page: 9 year: 2015 ident: CR27 article-title: Lwering the quantification limit of the QubitTM RNA HS Assay using RNA spike-in publication-title: BMC Mol. Biol. doi: 10.1186/s12867-015-0039-3 – volume: 6 start-page: 19529 year: 2016 ident: CR23 article-title: Assessing cellular and circulating miRNA recovery: the impact of the RNA isolation method and the quantity of input material publication-title: Sci. Rep. doi: 10.1038/srep19529 – volume: 18 start-page: 371 year: 2014 end-page: 90 ident: CR6 article-title: Methodological challenges in utilizing miRNAs as circulating biomarkers publication-title: J. Cell. Mol. Med. doi: 10.1111/jcmm.12236 – ident: CR28 – volume: 8 start-page: e64795 year: 2013 ident: CR18 article-title: Plasma processing conditions substantially influence circulating microRNA biomarker levels publication-title: PLoS One doi: 10.1371/journal.pone.0064795 – volume: 22 start-page: 59 year: 2013 end-page: 64 ident: CR24 article-title: Expression analysis on archival material revisited: isolation and quantification of RNA extracted from FFPE samples publication-title: Diagn. Mol. Pathol. doi: 10.1097/PDM.0b013e318269de3b – volume: 56 start-page: 998 year: 2010 end-page: 1006 ident: CR13 article-title: Robust microRNA stability in degraded RNA preparations from human tissue and cell samples publication-title: Clin. Chem. doi: 10.1373/clinchem.2009.141580 – volume: 110 start-page: 483 year: 2012 ident: 8134_CR10 publication-title: Circ. Res. doi: 10.1161/CIRCRESAHA.111.247452 – ident: 8134_CR28 doi: 10.1007/s00216-015-8846-4 – volume: 465 start-page: 179 year: 2014 ident: 8134_CR26 publication-title: Anal. Biochem. doi: 10.1016/j.ab.2014.08.006 – volume: 50 start-page: 298 year: 2010 ident: 8134_CR7 publication-title: Methods doi: 10.1016/j.ymeth.2010.01.032 – volume: 19 start-page: 1568 year: 2014 ident: 8134_CR29 publication-title: Molecules doi: 10.3390/molecules19021568 – volume: 11 start-page: 809 year: 2014 ident: 8134_CR15 publication-title: Nat. Methods doi: 10.1038/nmeth.3014 – volume: 431 start-page: 69 year: 2012 ident: 8134_CR5 publication-title: Anal. Biochem. doi: 10.1016/j.ab.2012.09.007 – volume: 15 start-page: 395 year: 2014 ident: 8134_CR20 publication-title: BMC Genomics doi: 10.1186/1471-2164-15-395 – volume: 16 start-page: 9 year: 2015 ident: 8134_CR27 publication-title: BMC Mol. Biol. doi: 10.1186/s12867-015-0039-3 – volume: 19 start-page: 712 year: 2013 ident: 8134_CR9 publication-title: RNA doi: 10.1261/rna.036863.112 – volume: 12 start-page: 435 year: 2011 ident: 8134_CR14 publication-title: BMC Genomics doi: 10.1186/1471-2164-12-435 – ident: 8134_CR19 doi: 10.1373/clinchem.2014.221341 – ident: 8134_CR17 doi: 10.1007/s11684-014-0379-2 – volume: 13 start-page: 358 year: 2012 ident: 8134_CR25 publication-title: Nat. Rev. Genet. doi: 10.1038/nrg3198 – volume: 18 start-page: 371 year: 2014 ident: 8134_CR6 publication-title: J. Cell. Mol. Med. doi: 10.1111/jcmm.12236 – volume: 22 start-page: 59 year: 2013 ident: 8134_CR24 publication-title: Diagn. Mol. Pathol. doi: 10.1097/PDM.0b013e318269de3b – volume: 136 start-page: 215 year: 2009 ident: 8134_CR1 publication-title: Cell doi: 10.1016/j.cell.2009.01.002 – volume: 59 start-page: 164 year: 2013 ident: 8134_CR4 publication-title: Methods doi: 10.1016/j.ymeth.2012.09.015 – volume: 105 start-page: 10513 year: 2008 ident: 8134_CR11 publication-title: Proc. Natl. Acad. Sci. U. S. A. doi: 10.1073/pnas.0804549105 – volume: 6 start-page: e20769 year: 2011 ident: 8134_CR12 publication-title: PLoS One doi: 10.1371/journal.pone.0020769 – volume: 8 start-page: e64795 year: 2013 ident: 8134_CR18 publication-title: PLoS One doi: 10.1371/journal.pone.0064795 – volume: 8 start-page: 467 year: 2011 ident: 8134_CR8 publication-title: Nat. Rev. Clin. Oncol. doi: 10.1038/nrclinonc.2011.76 – volume: 79 start-page: 351 year: 2010 ident: 8134_CR2 publication-title: Annu. Rev. Biochem. doi: 10.1146/annurev-biochem-060308-103103 – volume: 7 start-page: e41561 year: 2012 ident: 8134_CR21 publication-title: PLoS One doi: 10.1371/journal.pone.0041561 – volume: 81C start-page: 75 year: 2015 ident: 8134_CR16 publication-title: Adv. Drug Deliv. Rev. doi: 10.1016/j.addr.2014.09.001 – volume: 120 start-page: 15 year: 2005 ident: 8134_CR3 publication-title: Cell doi: 10.1016/j.cell.2004.12.035 – volume: 6 start-page: 19529 year: 2016 ident: 8134_CR23 publication-title: Sci. Rep. doi: 10.1038/srep19529 – volume: 56 start-page: 998 year: 2010 ident: 8134_CR13 publication-title: Clin. Chem. doi: 10.1373/clinchem.2009.141580 – volume: 8 start-page: e82753 year: 2013 ident: 8134_CR22 publication-title: PLoS One doi: 10.1371/journal.pone.0082753
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Title	Defining quantification methods and optimizing protocols for microarray hybridization of circulating microRNAs
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