Functional Dynamics of the Gut Microbiome in Elderly People during Probiotic Consumption

A mechanistic understanding of the purported health benefits conferred by consumption of probiotic bacteria has been limited by our knowledge of the resident gut microbiota and its interaction with the host. Here, we detail the impact of a single-organism probiotic, Lactobacillus rhamnosus GG ATCC 5...

Celý popis

Uložené v:

Podrobná bibliografia
Vydané v:	mBio Ročník 6; číslo 2
Hlavní autori:	Eloe-Fadrosh, Emiley A., Brady, Arthur, Crabtree, Jonathan, Drabek, Elliott F., Ma, Bing, Mahurkar, Anup, Ravel, Jacques, Haverkamp, Miriam, Fiorino, Anne-Maria, Botelho, Christine, Andreyeva, Irina, Hibberd, Patricia L., Fraser, Claire M.
Médium:	Journal Article
Jazyk:	English
Vydavateľské údaje:	United States American Society for Microbiology 01.05.2015 American Society of Microbiology
Predmet:	adhesion Aged Aged, 80 and over Bacteria Bifidobacterium Biofilms Bioinformatics butyrates Chemotaxis clinical trials Cluster Analysis Community composition community structure Datasets Digestive system DNA, Bacterial - chemistry DNA, Bacterial - genetics DNA, Ribosomal - chemistry DNA, Ribosomal - genetics elderly Epithelium Eubacterium (genus) Feces Female Flagella Gastric motility Gastrointestinal Microbiome Gastrointestinal tract Gastrointestinal Tract - microbiology Gene expression Gene Expression Profiling genes Genomes Gut microbiota human health Humans Intestinal microflora intestinal microorganisms Kinases Lactobacillus rhamnosus Lactobacillus rhamnosus - growth & development Male Microbial Interactions microbiome Microbiomes Microbiota Microorganisms Molecular Sequence Data Older people people Phylogenetics Phylogeny Probiotics Probiotics - administration & dosage Proteins ribosomal RNA RNA, Ribosomal, 16S - genetics Roseburia rRNA 16S Sequence Analysis, DNA Transcription transcription (genetics) transcriptome Transcriptomes Variance analysis
ISSN:	2161-2129, 2150-7511, 2150-7511
On-line prístup:	Získať plný text
Tagy:	Pridať tag Žiadne tagy, Buďte prvý, kto otaguje tento záznam!

Popis
Shrnutí:	A mechanistic understanding of the purported health benefits conferred by consumption of probiotic bacteria has been limited by our knowledge of the resident gut microbiota and its interaction with the host. Here, we detail the impact of a single-organism probiotic, Lactobacillus rhamnosus GG ATCC 53103 (LGG), on the structure and functional dynamics (gene expression) of the gut microbiota in a study of 12 healthy individuals, 65 to 80 years old. The analysis revealed that while the overall community composition was stable as assessed by 16S rRNA profiling, the transcriptional response of the gut microbiota was modulated by probiotic treatment. Comparison of transcriptional profiles based on taxonomic composition yielded three distinct transcriptome groups that displayed considerable differences in functional dynamics. The transcriptional profile of LGG in vivo was remarkably concordant across study subjects despite the considerable interindividual nature of the gut microbiota. However, we identified genes involved in flagellar motility, chemotaxis, and adhesion from Bifidobacterium and the dominant butyrate producers Roseburia and Eubacterium whose expression was increased during probiotic consumption, suggesting that LGG may promote interactions between key constituents of the microbiota and the host epithelium. These results provide evidence for the discrete functional effects imparted by a specific single-organism probiotic and challenge the prevailing notion that probiotics substantially modify the resident microbiota within nondiseased individuals in an appreciable fashion. IMPORTANCE Probiotic bacteria have been used for over a century to promote digestive health. Many individuals report that probiotics alleviate a number of digestive issues, yet little evidence links how probiotic microbes influence human health. Here, we show how the resident microbes that inhabit the healthy human gut respond to a probiotic. The well-studied probiotic Lactobacillus rhamnosus GG ATCC 53103 (LGG) was administered in a clinical trial, and a suite of measurements of the resident microbes were taken to evaluate potential changes over the course of probiotic consumption. We found that LGG transiently enriches for functions to potentially promote anti-inflammatory pathways in the resident microbes. Probiotic bacteria have been used for over a century to promote digestive health. Many individuals report that probiotics alleviate a number of digestive issues, yet little evidence links how probiotic microbes influence human health. Here, we show how the resident microbes that inhabit the healthy human gut respond to a probiotic. The well-studied probiotic Lactobacillus rhamnosus GG ATCC 53103 (LGG) was administered in a clinical trial, and a suite of measurements of the resident microbes were taken to evaluate potential changes over the course of probiotic consumption. We found that LGG transiently enriches for functions to potentially promote anti-inflammatory pathways in the resident microbes.
Bibliografia:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 ObjectType-Article-2 ObjectType-Feature-1 content type line 23 This article is a direct contribution from a Fellow of the American Academy of Microbiology. P.L.H. and C.M.F. contributed equally to this work.
ISSN:	2161-2129 2150-7511 2150-7511
DOI:	10.1128/mBio.00231-15