Environmental DNA detection tracks established seasonal occurrence of blacktip sharks (Carcharhinus limbatus) in a semi-enclosed subtropical bay

The integration of eDNA analysis into the population assessment and monitoring of sharks could greatly improve temporal and spatial data used for management purposes. This study aimed to compare eDNA detection against well-established seasonal changes in blacktip shark ( Carcharhinus limbatus ) abun...

Full description

Saved in:

Bibliographic Details
Published in:	Scientific reports Vol. 10; no. 1; p. 11847
Main Authors:	Postaire, Bautisse D., Bakker, Judith, Gardiner, Jayne, Wiley, Tonya R., Chapman, Demian D.
Format:	Journal Article
Language:	English
Published:	London Nature Publishing Group UK 16.07.2020 Nature Publishing Group
Subjects:	631/158/2039 631/158/2452 631/158/672 Animals Aquatic habitats Bays Carcharhinus limbatus Carcharhinus melanopterus Environmental assessment Environmental DNA Environmental DNA - analysis Environmental DNA - genetics Environmental monitoring Female Fish populations Florida Humanities and Social Sciences Male multidisciplinary Polymerase chain reaction Population Dynamics Real-Time Polymerase Chain Reaction Reproduction - genetics Sampling Science Science (multidisciplinary) Seasonal variations Seasons Seawater - analysis Sharks Sharks - genetics Species Specificity Tropical Climate Water analysis Water sampling Water temperature Florida
ISSN:	2045-2322, 2045-2322
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Abstract	The integration of eDNA analysis into the population assessment and monitoring of sharks could greatly improve temporal and spatial data used for management purposes. This study aimed to compare eDNA detection against well-established seasonal changes in blacktip shark ( Carcharhinus limbatus ) abundance in Terra Ceia Bay (FL, USA). We used a species-specific real-time PCR approach to detect C. limbatus eDNA in the bay on a near monthly basis from spring through mid-fall in 2018 and 2019. Previous studies have shown that C. limbatus give birth in the bay in early summer and immature sharks occur there until late fall, when decreasing water temperatures cause them to move offshore and southwards. Water samples (2 L) were collected (4–6 per month) and filtered in the field, with each then being subjected to real-time PCR. Carcharhinus limbatus ‘positive’ filters were significantly more commonly collected during the April-July sampling period than during the August-October sampling period. While following the predicted pattern, eDNA concentration was generally too low for accurate quantification. Our results show that C. limbatus eDNA detection follows known seasonal residency patterns consistently over 2 years of monitoring. Species-specific eDNA analysis using real-time PCR could therefore represent a cost-effective, scalable sampling tool to facilitate improved shark population monitoring in semi-enclosed marine habitats.
AbstractList	The integration of eDNA analysis into the population assessment and monitoring of sharks could greatly improve temporal and spatial data used for management purposes. This study aimed to compare eDNA detection against well-established seasonal changes in blacktip shark ( Carcharhinus limbatus ) abundance in Terra Ceia Bay (FL, USA). We used a species-specific real-time PCR approach to detect C. limbatus eDNA in the bay on a near monthly basis from spring through mid-fall in 2018 and 2019. Previous studies have shown that C. limbatus give birth in the bay in early summer and immature sharks occur there until late fall, when decreasing water temperatures cause them to move offshore and southwards. Water samples (2 L) were collected (4–6 per month) and filtered in the field, with each then being subjected to real-time PCR. Carcharhinus limbatus ‘positive’ filters were significantly more commonly collected during the April-July sampling period than during the August-October sampling period. While following the predicted pattern, eDNA concentration was generally too low for accurate quantification. Our results show that C. limbatus eDNA detection follows known seasonal residency patterns consistently over 2 years of monitoring. Species-specific eDNA analysis using real-time PCR could therefore represent a cost-effective, scalable sampling tool to facilitate improved shark population monitoring in semi-enclosed marine habitats. The integration of eDNA analysis into the population assessment and monitoring of sharks could greatly improve temporal and spatial data used for management purposes. This study aimed to compare eDNA detection against well-established seasonal changes in blacktip shark (Carcharhinus limbatus) abundance in Terra Ceia Bay (FL, USA). We used a species-specific real-time PCR approach to detect C. limbatus eDNA in the bay on a near monthly basis from spring through mid-fall in 2018 and 2019. Previous studies have shown that C. limbatus give birth in the bay in early summer and immature sharks occur there until late fall, when decreasing water temperatures cause them to move offshore and southwards. Water samples (2 L) were collected (4-6 per month) and filtered in the field, with each then being subjected to real-time PCR. Carcharhinus limbatus 'positive' filters were significantly more commonly collected during the April-July sampling period than during the August-October sampling period. While following the predicted pattern, eDNA concentration was generally too low for accurate quantification. Our results show that C. limbatus eDNA detection follows known seasonal residency patterns consistently over 2 years of monitoring. Species-specific eDNA analysis using real-time PCR could therefore represent a cost-effective, scalable sampling tool to facilitate improved shark population monitoring in semi-enclosed marine habitats.The integration of eDNA analysis into the population assessment and monitoring of sharks could greatly improve temporal and spatial data used for management purposes. This study aimed to compare eDNA detection against well-established seasonal changes in blacktip shark (Carcharhinus limbatus) abundance in Terra Ceia Bay (FL, USA). We used a species-specific real-time PCR approach to detect C. limbatus eDNA in the bay on a near monthly basis from spring through mid-fall in 2018 and 2019. Previous studies have shown that C. limbatus give birth in the bay in early summer and immature sharks occur there until late fall, when decreasing water temperatures cause them to move offshore and southwards. Water samples (2 L) were collected (4-6 per month) and filtered in the field, with each then being subjected to real-time PCR. Carcharhinus limbatus 'positive' filters were significantly more commonly collected during the April-July sampling period than during the August-October sampling period. While following the predicted pattern, eDNA concentration was generally too low for accurate quantification. Our results show that C. limbatus eDNA detection follows known seasonal residency patterns consistently over 2 years of monitoring. Species-specific eDNA analysis using real-time PCR could therefore represent a cost-effective, scalable sampling tool to facilitate improved shark population monitoring in semi-enclosed marine habitats. The integration of eDNA analysis into the population assessment and monitoring of sharks could greatly improve temporal and spatial data used for management purposes. This study aimed to compare eDNA detection against well-established seasonal changes in blacktip shark (Carcharhinus limbatus) abundance in Terra Ceia Bay (FL, USA). We used a species-specific real-time PCR approach to detect C. limbatus eDNA in the bay on a near monthly basis from spring through mid-fall in 2018 and 2019. Previous studies have shown that C. limbatus give birth in the bay in early summer and immature sharks occur there until late fall, when decreasing water temperatures cause them to move offshore and southwards. Water samples (2 L) were collected (4-6 per month) and filtered in the field, with each then being subjected to real-time PCR. Carcharhinus limbatus 'positive' filters were significantly more commonly collected during the April-July sampling period than during the August-October sampling period. While following the predicted pattern, eDNA concentration was generally too low for accurate quantification. Our results show that C. limbatus eDNA detection follows known seasonal residency patterns consistently over 2 years of monitoring. Species-specific eDNA analysis using real-time PCR could therefore represent a cost-effective, scalable sampling tool to facilitate improved shark population monitoring in semi-enclosed marine habitats. The integration of eDNA analysis into the population assessment and monitoring of sharks could greatly improve temporal and spatial data used for management purposes. This study aimed to compare eDNA detection against well-established seasonal changes in blacktip shark (Carcharhinus limbatus) abundance in Terra Ceia Bay (FL, USA). We used a species-specific real-time PCR approach to detect C. limbatus eDNA in the bay on a near monthly basis from spring through mid-fall in 2018 and 2019. Previous studies have shown that C. limbatus give birth in the bay in early summer and immature sharks occur there until late fall, when decreasing water temperatures cause them to move offshore and southwards. Water samples (2 L) were collected (4–6 per month) and filtered in the field, with each then being subjected to real-time PCR. Carcharhinus limbatus ‘positive’ filters were significantly more commonly collected during the April-July sampling period than during the August-October sampling period. While following the predicted pattern, eDNA concentration was generally too low for accurate quantification. Our results show that C. limbatus eDNA detection follows known seasonal residency patterns consistently over 2 years of monitoring. Species-specific eDNA analysis using real-time PCR could therefore represent a cost-effective, scalable sampling tool to facilitate improved shark population monitoring in semi-enclosed marine habitats.
ArticleNumber	11847
Author	Bakker, Judith Postaire, Bautisse D. Chapman, Demian D. Gardiner, Jayne Wiley, Tonya R.
Author_xml	– sequence: 1 givenname: Bautisse D. surname: Postaire fullname: Postaire, Bautisse D. email: postaireb@gmail.com organization: Department of Biological Sciences, Florida International University – sequence: 2 givenname: Judith surname: Bakker fullname: Bakker, Judith organization: Department of Biological Sciences, Florida International University – sequence: 3 givenname: Jayne surname: Gardiner fullname: Gardiner, Jayne organization: Division of Natural Sciences, New College of Florida – sequence: 4 givenname: Tonya R. surname: Wiley fullname: Wiley, Tonya R. organization: Havenworth Coastal Conservation – sequence: 5 givenname: Demian D. surname: Chapman fullname: Chapman, Demian D. organization: Department of Biological Sciences, Florida International University
BackLink	https://www.ncbi.nlm.nih.gov/pubmed/32678294$$D View this record in MEDLINE/PubMed
BookMark	eNp9kk1vFSEUhompsR_2D7gwJG7axSifM8PGpLnWj6TRja4Jw3B7qQxcgWnSf9Gf7LlOq7WLsgHC8755Oeccor2YokPoFSVvKeH9uyKoVH1DGGnavhe8Ic_QASNCNowztvfgvI-OS7kisCRTgqoXaJ-ztuvhcoBuz-O1zylOLlYT8IevZ3h01dnqU8Q1G_uzYFeqGYIvGzfi4kxJEchk7Zyzi9bhtMZDALL6LS4bk0FysjLZwnHj41xw8NNg6lxOsY_YgMfkG1CGVHaO81Bz2noLpoO5eYmer00o7vhuP0I_Pp5_X31uLr59-rI6u2is7FRtmLSjE1J1VkklBie569ddOyju2qGn0nSEdXyUko7SCmlUz5ld065lrWOCMH6E3i--23mY3Gjh_9kEvc1-MvlGJ-P1_y_Rb_RlutYdbzvWKzA4uTPI6dcMNdKTL9aFYKJLc9FMMKGUoqoF9M0j9CrNGaq4UHxHUqBeP0z0N8p9swDoF8DmVEp2a219NbtOQUAfNCV6Nxp6GQ0No6H_jIYmIGWPpPfuT4r4IioAx0uX_8V-QvUbGJnNhA
CitedBy_id	crossref_primary_10_1002_nafm_10937 crossref_primary_10_1016_j_ecolind_2023_110795 crossref_primary_10_3897_mbmg_8_128689 crossref_primary_10_1051_e3sconf_202344201021 crossref_primary_10_1016_j_ecolind_2021_107649 crossref_primary_10_1111_jfb_15406 crossref_primary_10_1016_j_biocon_2022_109881 crossref_primary_10_24319_jtpk_16_313_322 crossref_primary_10_1016_j_ecolind_2023_110718 crossref_primary_10_3389_fmicb_2022_1026596 crossref_primary_10_1007_s00227_023_04193_5 crossref_primary_10_3389_fmars_2022_890756 crossref_primary_10_1016_j_fishres_2022_106259 crossref_primary_10_1016_j_rsma_2022_102661 crossref_primary_10_1016_j_marenvres_2023_106239 crossref_primary_10_3389_fmars_2022_945758 crossref_primary_10_1002_aqc_3591 crossref_primary_10_3389_fmars_2024_1282091 crossref_primary_10_1016_j_marpolbul_2025_118026 crossref_primary_10_7717_peerj_14071 crossref_primary_10_1093_mcfafs_vtaf007 crossref_primary_10_1002_edn3_201 crossref_primary_10_1002_aqc_3954 crossref_primary_10_3389_fmars_2023_1033258 crossref_primary_10_1111_csp2_407
Cites_doi	10.1101/765321 10.1002/edn3.29 10.1002/edn3.49 10.1038/s41598-017-17150-2 10.1007/978-94-017-1402-0_10 10.7554/eLife.00590 10.1016/j.jembe.2018.09.004 10.1007/s00227-017-3141-x 10.3389/fmars.2018.00355 10.1111/1755-0998.12483 10.1021/ac202028g 10.1111/cobi.13043 10.1016/j.biocon.2014.11.017 10.1111/cobi.13183 10.1038/srep40368 10.1038/srep46294 10.1126/science.303.5656.285 10.1111/2041-210X.13201 10.1111/ele.12162 10.1111/j.1365-294X.2012.05542.x 10.1111/1755-0998.12982 10.1111/j.1365-294X.2006.03036.x 10.1098/rsos.150088 10.1111/fwb.12846 10.1111/mec.13481 10.1038/s41598-018-34663-6 10.1126/sciadv.aap9661 10.1093/nar/gkh340 10.1038/s42003-019-0696-8 10.1016/j.fishres.2014.08.022 10.1016/j.marpol.2012.12.034 10.1016/j.cub.2006.09.044 10.1111/j.1365-294X.2012.05600.x 10.1371/journal.pone.0011968 10.1023/B:EBFI.0000045710.18997.f7 10.3389/fmicb.2017.00108 10.3354/esr00731 10.1111/1755-0998.12619 10.1111/1755-0998.12522 10.1111/1755-0998.12987 10.1139/f02-036 10.1080/19425120.2012.758204 10.7717/peerj.3044 10.3389/fmars.2018.00133
ContentType	Journal Article
Copyright	The Author(s) 2020 The Author(s) 2020. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.
Copyright_xml	– notice: The Author(s) 2020 – notice: The Author(s) 2020. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.
DBID	C6C AAYXX CITATION CGR CUY CVF ECM EIF NPM 3V. 7X7 7XB 88A 88E 88I 8FE 8FH 8FI 8FJ 8FK ABUWG AEUYN AFKRA AZQEC BBNVY BENPR BHPHI CCPQU DWQXO FYUFA GHDGH GNUQQ HCIFZ K9. LK8 M0S M1P M2P M7P PHGZM PHGZT PIMPY PJZUB PKEHL PPXIY PQEST PQGLB PQQKQ PQUKI Q9U 7X8 5PM
DOI	10.1038/s41598-020-68843-0
DatabaseName	Springer Nature OA Free Journals CrossRef Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed ProQuest Central (Corporate) Health & Medical Collection ProQuest Central (purchase pre-March 2016) Biology Database (Alumni Edition) Medical Database (Alumni Edition) Science Database (Alumni Edition) ProQuest SciTech Collection ProQuest Natural Science Collection ProQuest Hospital Collection Hospital Premium Collection (Alumni Edition) ProQuest Central (Alumni) (purchase pre-March 2016) ProQuest Central (Alumni) ProQuest One Sustainability ProQuest Central UK/Ireland ProQuest Central Essentials - QC Biological Science Collection ProQuest Central Natural Science Collection ProQuest One ProQuest Central Proquest Health Research Premium Collection Health Research Premium Collection (Alumni) ProQuest Central Student SciTech Premium Collection (via ProQuest) ProQuest Health & Medical Complete (Alumni) Biological Sciences ProQuest Health & Medical Collection Medical Database Science Database (via ProQuest SciTech Premium Collection) Biological Science Database ProQuest Central Premium ProQuest One Academic (New) Publicly Available Content Database ProQuest Health & Medical Research Collection ProQuest One Academic Middle East (New) ProQuest One Health & Nursing ProQuest One Academic Eastern Edition (DO NOT USE) ProQuest One Applied & Life Sciences ProQuest One Academic (retired) ProQuest One Academic UKI Edition ProQuest Central Basic MEDLINE - Academic PubMed Central (Full Participant titles)
DatabaseTitle	CrossRef MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) Publicly Available Content Database ProQuest Central Student ProQuest One Academic Middle East (New) ProQuest Central Essentials ProQuest Health & Medical Complete (Alumni) ProQuest Central (Alumni Edition) SciTech Premium Collection ProQuest One Community College ProQuest One Health & Nursing ProQuest Natural Science Collection ProQuest Biology Journals (Alumni Edition) ProQuest Central ProQuest One Applied & Life Sciences ProQuest One Sustainability ProQuest Health & Medical Research Collection Health Research Premium Collection Health and Medicine Complete (Alumni Edition) Natural Science Collection ProQuest Central Korea Health & Medical Research Collection Biological Science Collection ProQuest Central (New) ProQuest Medical Library (Alumni) ProQuest Science Journals (Alumni Edition) ProQuest Biological Science Collection ProQuest Central Basic ProQuest Science Journals ProQuest One Academic Eastern Edition ProQuest Hospital Collection Health Research Premium Collection (Alumni) Biological Science Database ProQuest SciTech Collection ProQuest Hospital Collection (Alumni) ProQuest Health & Medical Complete ProQuest Medical Library ProQuest One Academic UKI Edition ProQuest One Academic ProQuest One Academic (New) ProQuest Central (Alumni) MEDLINE - Academic
DatabaseTitleList	CrossRef MEDLINE - Academic MEDLINE Publicly Available Content Database
Database_xml	– sequence: 1 dbid: NPM name: PubMed url: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: PIMPY name: Publicly Available Content Database url: http://search.proquest.com/publiccontent sourceTypes: Aggregation Database
DeliveryMethod	fulltext_linktorsrc
Discipline	Biology
EISSN	2045-2322
ExternalDocumentID	PMC7367289 32678294 10_1038_s41598_020_68843_0
Genre	Research Support, Non-U.S. Gov't Journal Article
GeographicLocations	Florida
GeographicLocations_xml	– name: Florida
GrantInformation_xml	– fundername: Paul G. Allen Family Foundation funderid: http://dx.doi.org/10.13039/100000952 – fundername: Shark Conservation Fund – fundername: ;
GroupedDBID	0R~ 3V. 4.4 53G 5VS 7X7 88A 88E 88I 8FE 8FH 8FI 8FJ AAFWJ AAJSJ AAKDD ABDBF ABUWG ACGFS ACSMW ACUHS ADBBV ADRAZ AENEX AEUYN AFKRA AJTQC ALIPV ALMA_UNASSIGNED_HOLDINGS AOIJS AZQEC BAWUL BBNVY BCNDV BENPR BHPHI BPHCQ BVXVI C6C CCPQU DIK DWQXO EBD EBLON EBS ESX FYUFA GNUQQ GROUPED_DOAJ GX1 HCIFZ HH5 HMCUK HYE KQ8 LK8 M0L M1P M2P M48 M7P M~E NAO OK1 PIMPY PQQKQ PROAC PSQYO RNT RNTTT RPM SNYQT UKHRP AASML AAYXX AFFHD AFPKN CITATION PHGZM PHGZT PJZUB PPXIY PQGLB CGR CUY CVF ECM EIF NPM 7XB 8FK K9. PKEHL PQEST PQUKI Q9U 7X8 PUEGO 5PM
ID	FETCH-LOGICAL-c579t-25cde4597c9594be53e8f76b93e6b815a70273d551d5c45a9832cf17626e24023
IEDL.DBID	M7P
ISICitedReferencesCount	25
ISICitedReferencesURI	http://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=Summon&SrcAuth=ProQuest&DestLinkType=CitingArticles&DestApp=WOS_CPL&KeyUT=000550057200160&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D
ISSN	2045-2322
IngestDate	Tue Nov 04 01:48:13 EST 2025 Thu Sep 04 16:42:44 EDT 2025 Tue Oct 07 07:37:59 EDT 2025 Thu Jan 02 22:58:02 EST 2025 Sat Nov 29 05:33:07 EST 2025 Tue Nov 18 22:06:33 EST 2025 Fri Feb 21 02:38:48 EST 2025
IsDoiOpenAccess	true
IsOpenAccess	true
IsPeerReviewed	true
IsScholarly	true
Issue	1
Language	English
License	Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
LinkModel	DirectLink
MergedId	FETCHMERGED-LOGICAL-c579t-25cde4597c9594be53e8f76b93e6b815a70273d551d5c45a9832cf17626e24023
Notes	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23
OpenAccessLink	https://www.proquest.com/docview/2424342491?pq-origsite=%requestingapplication%
PMID	32678294
PQID	2424342491
PQPubID	2041939
ParticipantIDs	pubmedcentral_primary_oai_pubmedcentral_nih_gov_7367289 proquest_miscellaneous_2424999196 proquest_journals_2424342491 pubmed_primary_32678294 crossref_citationtrail_10_1038_s41598_020_68843_0 crossref_primary_10_1038_s41598_020_68843_0 springer_journals_10_1038_s41598_020_68843_0
PublicationCentury	2000
PublicationDate	2020-07-16
PublicationDateYYYYMMDD	2020-07-16
PublicationDate_xml	– month: 07 year: 2020 text: 2020-07-16 day: 16
PublicationDecade	2020
PublicationPlace	London
PublicationPlace_xml	– name: London – name: England
PublicationTitle	Scientific reports
PublicationTitleAbbrev	Sci Rep
PublicationTitleAlternate	Sci Rep
PublicationYear	2020
Publisher	Nature Publishing Group UK Nature Publishing Group
Publisher_xml	– name: Nature Publishing Group UK – name: Nature Publishing Group
References	Worm (CR5) 2013; 40 Killam, Parsons (CR40) 1989; 87 Ji (CR14) 2013; 16 Ward-Paige (CR18) 2010; 5 Compagno, Dando, Fowler (CR35) 2005 CR33 Sigsgaard (CR24) 2016; 1 Cardeñosa, Quinlan, Shea, Chapman (CR38) 2018; 8 Truelove, Andruszkiewicz, Block (CR25) 2019; 10 O’Donnell (CR47) 2017; 5 Jerde (CR17) 2019; 13 Turner, Uy, Everhart (CR43) 2015; 183 Miya (CR12) 2015; 2 Salter, Joensen, Kristiansen, Steingrund, Vestergaard (CR32) 2019; 2 Edgar (CR45) 2004; 32 Yamamoto (CR48) 2017; 7 Castro (CR34) 1996; 59 Klymus (CR46) 2019 Lehman (CR55) 2019 Lacoursiere-Roussel, Rosabal, Bernatchez (CR16) 2016; 16 Weltz (CR21) 2017; 12 Keeney, Heist (CR36) 2006; 15 Lafferty, Benesh, Mahon, Jerde, Lowe (CR23) 2018; 5 Heupel, Hueter, Sibert, Nielsen (CR42) 2001 Buxton (CR28) 2017; 7 Fields (CR37) 2018; 32 Yamamoto (CR30) 2016; 11 Levi (CR31) 2019; 19 Hunter (CR52) 2017; 17 Camhi, Valenti, Fordham, Fowler, Gibson (CR3) 2009 Heupel, Simpfendorfer, Hueter (CR41) 2004; 71 Robbins, Hisano, Connolly, Choat (CR2) 2006; 16 Simpfendorfer (CR10) 2016; 30 Knudsen (CR44) 2019; 510 Stat (CR50) 2019; 33 Hindson (CR54) 2011; 83 Wheeler (CR8) 2004; 303 Boussarie (CR27) 2018; 4 Baremore, Passerotti (CR39) 2013; 5 Lodge (CR9) 2012; 21 Bakker (CR26) 2017 Doi (CR29) 2017; 62 Jeunen (CR49) 2019; 19 Baker, Steel, Nieukirk, Klinck (CR11) 2018; 5 Dulvy (CR7) 2014; 3 Spaet, Berumen (CR6) 2015; 161 Port (CR13) 2016; 25 Ward-Paige (CR4) 2010; 5 Schweiss, Lehman, Drymon, Phillips (CR20) 2019; 2 Kralik, Ricchi (CR53) 2017; 8 Jeunen (CR51) 2019 Gargan (CR22) 2017; 164 Taberlet, Coissac, Hajibabaei, Rieseberg (CR15) 2012; 21 Heupel, Simpfendorfer (CR1) 2002; 59 Furlan, Gleeson, Hardy, Duncan (CR19) 2016; 16 MD Camhi (68843_CR3) 2009 CS Baker (68843_CR11) 2018; 5 DB Keeney (68843_CR36) 2006; 15 68843_CR33 KE Schweiss (68843_CR20) 2019; 2 MR Heupel (68843_CR41) 2004; 71 J Bakker (68843_CR26) 2017 Y Ji (68843_CR14) 2013; 16 AT Fields (68843_CR37) 2018; 32 NK Dulvy (68843_CR7) 2014; 3 B Worm (68843_CR5) 2013; 40 LM Gargan (68843_CR22) 2017; 164 KA Killam (68843_CR40) 1989; 87 ME Hunter (68843_CR52) 2017; 17 RC Edgar (68843_CR45) 2004; 32 IE Baremore (68843_CR39) 2013; 5 NK Truelove (68843_CR25) 2019; 10 EM Furlan (68843_CR19) 2016; 16 S Yamamoto (68843_CR48) 2017; 7 JLY Spaet (68843_CR6) 2015; 161 CA Ward-Paige (68843_CR4) 2010; 5 P Taberlet (68843_CR15) 2012; 21 M Miya (68843_CR12) 2015; 2 CL Jerde (68843_CR17) 2019; 13 P Kralik (68843_CR53) 2017; 8 KD Lafferty (68843_CR23) 2018; 5 QD Wheeler (68843_CR8) 2004; 303 GJ Jeunen (68843_CR49) 2019; 19 DM Lodge (68843_CR9) 2012; 21 S Yamamoto (68843_CR30) 2016; 11 I Salter (68843_CR32) 2019; 2 G Boussarie (68843_CR27) 2018; 4 SW Knudsen (68843_CR44) 2019; 510 WD Robbins (68843_CR2) 2006; 16 A Lacoursiere-Roussel (68843_CR16) 2016; 16 H Doi (68843_CR29) 2017; 62 CA Simpfendorfer (68843_CR10) 2016; 30 CR Turner (68843_CR43) 2015; 183 BJ Hindson (68843_CR54) 2011; 83 RN Lehman (68843_CR55) 2019 MR Heupel (68843_CR1) 2002; 59 MR Heupel (68843_CR42) 2001 JL O’Donnell (68843_CR47) 2017; 5 T Levi (68843_CR31) 2019; 19 D Cardeñosa (68843_CR38) 2018; 8 JI Castro (68843_CR34) 1996; 59 JA Port (68843_CR13) 2016; 25 EE Sigsgaard (68843_CR24) 2016; 1 M Stat (68843_CR50) 2019; 33 LJV Compagno (68843_CR35) 2005 AS Buxton (68843_CR28) 2017; 7 K Weltz (68843_CR21) 2017; 12 CA Ward-Paige (68843_CR18) 2010; 5 KE Klymus (68843_CR46) 2019 G Jeunen (68843_CR51) 2019
References_xml	– volume: 2 start-page: 20 year: 2015 ident: CR12 article-title: MiFish, a set of universal PCR primers for metabarcoding environmental DNA from fishes: Detection of more than 230 subtropical marine species publication-title: R. Soc. Open Sci. – volume: 5 start-page: e11968 year: 2010 ident: CR4 article-title: Large-scale absence of sharks on reefs in the greater-Caribbean: A footprint of human pressures publication-title: PLoS One – volume: 303 start-page: 285 year: 2004 end-page: 285 ident: CR8 article-title: Taxonomy: Impediment or expedient? publication-title: Science – volume: 8 start-page: 1 year: 2018 end-page: 10 ident: CR38 article-title: Multiplex real-time PCR assay to detect illegal trade of CITES-listed shark species publication-title: Sci. Rep. – volume: 16 start-page: 641 year: 2016 end-page: 654 ident: CR19 article-title: A framework for estimating the sensitivity of eDNA surveys publication-title: Mol. Ecol. Resour. – volume: 40 start-page: 194 year: 2013 end-page: 204 ident: CR5 article-title: Global catches, exploitation rates, and rebuilding options for sharks publication-title: Mar. Policy – year: 2019 ident: CR55 article-title: An environmental DNA tool for monitoring the status of the Critically Endangered Smalltooth Sawfish, , in the Western Atlantic publication-title: Conserv. Genet. Resour. doi: 10.1101/765321 – volume: 11 start-page: 1 year: 2016 end-page: 18 ident: CR30 article-title: Environmental DNA as a ‘snapshot’ of fish distribution: A case study of Japanese jack mackerel in Maizuru Bay, Sea of Japan publication-title: PLoS One – year: 2005 ident: CR35 publication-title: A Field Guide to the Sharks of the World – volume: 1 start-page: 1 year: 2016 end-page: 4 ident: CR24 article-title: Population characteristics of a large whale shark aggregation inferred from seawater environmental DNA publication-title: Nat. Publ. Gr. – volume: 3 start-page: e00590 year: 2014 ident: CR7 article-title: Extinction risk and conservation of the world’s sharks and rays publication-title: Elife – volume: 32 start-page: 1792 year: 2004 end-page: 1797 ident: CR45 article-title: MUSCLE: Multiple sequence alignment with high accuracy and high throughput publication-title: Nucleic Acids Res. – volume: 5 start-page: e3044 year: 2017 ident: CR47 article-title: Spatial distribution of environmental DNA in a nearshore marine habitat publication-title: PeerJ – volume: 33 start-page: 196 year: 2019 end-page: 205 ident: CR50 article-title: Combined use of eDNA metabarcoding and video surveillance for the assessment of fish biodiversity publication-title: Conserv. Biol. – volume: 161 start-page: 356 year: 2015 end-page: 364 ident: CR6 article-title: Fish market surveys indicate unsustainable elasmobranch fisheries in the Saudi Arabian Red Sea publication-title: Fish. Res. – year: 2019 ident: CR46 article-title: Reporting the limits of detection and quantification for environmental DNA assays publication-title: Environ. DNA doi: 10.1002/edn3.29 – volume: 17 start-page: 221 year: 2017 end-page: 229 ident: CR52 article-title: Detection limits of quantitative and digital PCR assays and their influence in presence-absence surveys of environmental DNA publication-title: Mol. Ecol. Resour. – volume: 5 start-page: 1 year: 2018 end-page: 11 ident: CR11 article-title: Environmental DNA (eDNA) from the wake of the whales: Droplet digital PCR for detection and species identification publication-title: Front. Mar. Sci. – volume: 71 start-page: 135 year: 2004 end-page: 142 ident: CR41 article-title: Estimation of shark home ranges using passive monitoring techniques publication-title: Environ. Biol. Fish. – volume: 62 start-page: 30 year: 2017 end-page: 39 ident: CR29 article-title: Environmental DNA analysis for estimating the abundance and biomass of stream fish publication-title: Freshw. Biol. – volume: 15 start-page: 3669 year: 2006 end-page: 3679 ident: CR36 article-title: Worldwide phylogeography of the blacktip shark ( ) inferred from mitochondrial DNA reveals isolation of western Atlantic populations coupled with recent Pacific dispersal publication-title: Mol. Ecol. – volume: 5 start-page: 127 year: 2013 end-page: 138 ident: CR39 article-title: Reproduction of the Blacktip Shark in the Gulf of Mexico publication-title: Mar. Coast. Fish. – volume: 5 start-page: 20 year: 2010 ident: CR18 article-title: Large-scale absence of sharks on reefs in the greater-Caribbean: A footprint of human pressures publication-title: PLoS One – volume: 7 start-page: 40368 year: 2017 ident: CR48 article-title: Environmental DNA metabarcoding reveals local fish communities in a species-rich coastal sea publication-title: Sci. Rep. – volume: 59 start-page: 508 year: 1996 end-page: 522 ident: CR34 article-title: Biology of the blacktip shark, , off the southeastern United States publication-title: Bull. Mar. Sci. – year: 2019 ident: CR51 article-title: Water stratification in the marine biome restricts vertical environmental DNA (eDNA) signal dispersal publication-title: Environ. DNA doi: 10.1002/edn3.49 – volume: 19 start-page: 426 year: 2019 end-page: 438 ident: CR49 article-title: Environmental DNA (eDNA) metabarcoding reveals strong discrimination among diverse marine habitats connected by water movement publication-title: Mol. Ecol. Resour. – volume: 5 start-page: 1 year: 2018 end-page: 6 ident: CR23 article-title: Detecting Southern California’s white sharks with environmental DNA publication-title: Front. Mar. Sci. – volume: 19 start-page: 597 year: 2019 end-page: 608 ident: CR31 article-title: Environmental DNA for the enumeration and management of Pacific salmon publication-title: Mol. Ecol. Resour. – volume: 16 start-page: 1245 year: 2013 end-page: 1257 ident: CR14 article-title: Reliable, verifiable and efficient monitoring of biodiversity via metabarcoding publication-title: Ecol. Lett. – volume: 4 start-page: 20 year: 2018 ident: CR27 article-title: Environmental DNA illuminates the dark diversity of sharks publication-title: Sci. Adv. – volume: 83 start-page: 8604 year: 2011 end-page: 8610 ident: CR54 article-title: High-throughput droplet digital PCR system for absolute quantitation of DNA copy number publication-title: Anal. Chem. – volume: 16 start-page: 1401 year: 2016 end-page: 1414 ident: CR16 article-title: Estimating fish abundance and biomass from eDNA concentrations: Variability among capture methods and environmental conditions publication-title: Mol. Ecol. Resour. – year: 2017 ident: CR26 article-title: Environmental DNA reveals tropical shark diversity and abundance in contrasting levels of anthropogenic impact publication-title: Sci. Rep. doi: 10.1038/s41598-017-17150-2 – volume: 87 start-page: 845 year: 1989 end-page: 857 ident: CR40 article-title: Age and growth of the blacktip shark, , near Tampa Bay, Florida publication-title: Fish. Bull. – volume: 16 start-page: 2314 year: 2006 end-page: 2319 ident: CR2 article-title: Ongoing collapse of coral-reef shark populations publication-title: Curr. Biol. – volume: 164 start-page: 112 year: 2017 ident: CR22 article-title: Development of a sensitive detection method to survey pelagic biodiversity using eDNA and quantitative PCR: A case study of devil ray at seamounts publication-title: Mar. Biol. – volume: 10 start-page: 1128 year: 2019 end-page: 1135 ident: CR25 article-title: A rapid environmental DNA method for detecting white sharks in the open ocean publication-title: Methods Ecol. Evol. – volume: 8 start-page: 1 year: 2017 end-page: 9 ident: CR53 article-title: A basic guide to real time PCR in microbial diagnostics: Definitions, parameters, and everything publication-title: Front. Microbiol. – volume: 21 start-page: 1789 year: 2012 end-page: 1793 ident: CR15 article-title: Environmental DNA publication-title: Mol. Ecol. – volume: 32 start-page: 376 year: 2018 end-page: 389 ident: CR37 article-title: Species composition of the international shark fin trade assessed through a retail-market survey in Hong Kong publication-title: Conserv. Biol. – volume: 7 start-page: 46294 year: 2017 ident: CR28 article-title: Seasonal variation in environmental DNA in relation to population size and environmental factors publication-title: Sci. Rep. – ident: CR33 – volume: 25 start-page: 527 year: 2016 end-page: 541 ident: CR13 article-title: Assessing vertebrate biodiversity in a kelp forest ecosystem using environmental DNA publication-title: Mol. Ecol. – volume: 2 start-page: 39 year: 2019 ident: CR20 article-title: Development of highly sensitive environmental DNA methods for the detection of Bull Sharks, (Müller and Henle, 1839), using Droplet Digital PCR publication-title: Environ. DNA – volume: 2 start-page: 461 year: 2019 ident: CR32 article-title: Environmental DNA concentrations are correlated with regional biomass of Atlantic cod in oceanic waters publication-title: Commun. Biol. – volume: 59 start-page: 624 year: 2002 end-page: 632 ident: CR1 article-title: Estimation of mortality of juvenile blacktip sharks, , within a nursery area using telemetry data publication-title: Can. J. Fish. Aquat. Sci. – volume: 183 start-page: 93 year: 2015 end-page: 102 ident: CR43 article-title: Fish environmental DNA is more concentrated in aquatic sediments than surface water publication-title: Biol. Conserv. – volume: 13 start-page: 287 year: 2019 end-page: 288 ident: CR17 article-title: Can we manage fisheries with the inherent uncertainty from eDNA? publication-title: J. Fish Biol. – volume: 510 start-page: 31 year: 2019 end-page: 45 ident: CR44 article-title: Species-specific detection and quantification of environmental DNA from marine fishes in the Baltic Sea publication-title: J. Exp. Mar. Bio. Ecol. – volume: 30 start-page: 109 year: 2016 end-page: 116 ident: CR10 article-title: Environmental DNA detects critically endangered largetooth sawfish in the wild publication-title: Endanger. Species Res. – start-page: 19 year: 2009 end-page: 23 ident: CR3 publication-title: The Conservation Status of Pelagic Sharks and Rays – volume: 21 start-page: 2555 year: 2012 end-page: 2558 ident: CR9 article-title: Conservation in a cup of water: Estimating biodiversity and population abundance from environmental DNA publication-title: Mol. Ecol. – start-page: 217 year: 2001 end-page: 236 ident: CR42 article-title: Use of an automated acoustic telemetry system to passively track Juvenile Blacktip Shark movements bib publication-title: Electronic Tagging and Tracking in Marine Fisheries doi: 10.1007/978-94-017-1402-0_10 – volume: 12 start-page: 1 year: 2017 end-page: 16 ident: CR21 article-title: Application of environmental DNA to detect an endangered marine skate species in the wild publication-title: PLoS One – volume: 3 start-page: e00590 year: 2014 ident: 68843_CR7 publication-title: Elife doi: 10.7554/eLife.00590 – volume: 510 start-page: 31 year: 2019 ident: 68843_CR44 publication-title: J. Exp. Mar. Bio. Ecol. doi: 10.1016/j.jembe.2018.09.004 – volume: 164 start-page: 112 year: 2017 ident: 68843_CR22 publication-title: Mar. Biol. doi: 10.1007/s00227-017-3141-x – volume: 2 start-page: 39 year: 2019 ident: 68843_CR20 publication-title: Environ. DNA – volume: 5 start-page: 1 year: 2018 ident: 68843_CR23 publication-title: Front. Mar. Sci. doi: 10.3389/fmars.2018.00355 – volume: 16 start-page: 641 year: 2016 ident: 68843_CR19 publication-title: Mol. Ecol. Resour. doi: 10.1111/1755-0998.12483 – volume: 83 start-page: 8604 year: 2011 ident: 68843_CR54 publication-title: Anal. Chem. doi: 10.1021/ac202028g – volume: 32 start-page: 376 year: 2018 ident: 68843_CR37 publication-title: Conserv. Biol. doi: 10.1111/cobi.13043 – volume: 183 start-page: 93 year: 2015 ident: 68843_CR43 publication-title: Biol. Conserv. doi: 10.1016/j.biocon.2014.11.017 – volume: 33 start-page: 196 year: 2019 ident: 68843_CR50 publication-title: Conserv. Biol. doi: 10.1111/cobi.13183 – year: 2017 ident: 68843_CR26 publication-title: Sci. Rep. doi: 10.1038/s41598-017-17150-2 – volume: 7 start-page: 40368 year: 2017 ident: 68843_CR48 publication-title: Sci. Rep. doi: 10.1038/srep40368 – volume: 7 start-page: 46294 year: 2017 ident: 68843_CR28 publication-title: Sci. Rep. doi: 10.1038/srep46294 – volume: 5 start-page: 20 year: 2010 ident: 68843_CR18 publication-title: PLoS One – volume: 303 start-page: 285 year: 2004 ident: 68843_CR8 publication-title: Science doi: 10.1126/science.303.5656.285 – volume: 1 start-page: 1 year: 2016 ident: 68843_CR24 publication-title: Nat. Publ. Gr. – volume: 13 start-page: 287 year: 2019 ident: 68843_CR17 publication-title: J. Fish Biol. – volume: 10 start-page: 1128 year: 2019 ident: 68843_CR25 publication-title: Methods Ecol. Evol. doi: 10.1111/2041-210X.13201 – volume: 16 start-page: 1245 year: 2013 ident: 68843_CR14 publication-title: Ecol. Lett. doi: 10.1111/ele.12162 – volume: 21 start-page: 1789 year: 2012 ident: 68843_CR15 publication-title: Mol. Ecol. doi: 10.1111/j.1365-294X.2012.05542.x – volume: 19 start-page: 426 year: 2019 ident: 68843_CR49 publication-title: Mol. Ecol. Resour. doi: 10.1111/1755-0998.12982 – volume: 15 start-page: 3669 year: 2006 ident: 68843_CR36 publication-title: Mol. Ecol. doi: 10.1111/j.1365-294X.2006.03036.x – volume: 2 start-page: 20 year: 2015 ident: 68843_CR12 publication-title: R. Soc. Open Sci. doi: 10.1098/rsos.150088 – volume: 62 start-page: 30 year: 2017 ident: 68843_CR29 publication-title: Freshw. Biol. doi: 10.1111/fwb.12846 – volume: 25 start-page: 527 year: 2016 ident: 68843_CR13 publication-title: Mol. Ecol. doi: 10.1111/mec.13481 – volume: 8 start-page: 1 year: 2018 ident: 68843_CR38 publication-title: Sci. Rep. doi: 10.1038/s41598-018-34663-6 – volume: 4 start-page: 20 year: 2018 ident: 68843_CR27 publication-title: Sci. Adv. doi: 10.1126/sciadv.aap9661 – volume: 32 start-page: 1792 year: 2004 ident: 68843_CR45 publication-title: Nucleic Acids Res. doi: 10.1093/nar/gkh340 – volume: 2 start-page: 461 year: 2019 ident: 68843_CR32 publication-title: Commun. Biol. doi: 10.1038/s42003-019-0696-8 – volume: 11 start-page: 1 year: 2016 ident: 68843_CR30 publication-title: PLoS One – ident: 68843_CR33 doi: 10.1007/978-94-017-1402-0_10 – volume: 161 start-page: 356 year: 2015 ident: 68843_CR6 publication-title: Fish. Res. doi: 10.1016/j.fishres.2014.08.022 – volume: 12 start-page: 1 year: 2017 ident: 68843_CR21 publication-title: PLoS One – volume: 40 start-page: 194 year: 2013 ident: 68843_CR5 publication-title: Mar. Policy doi: 10.1016/j.marpol.2012.12.034 – volume: 16 start-page: 2314 year: 2006 ident: 68843_CR2 publication-title: Curr. Biol. doi: 10.1016/j.cub.2006.09.044 – year: 2019 ident: 68843_CR46 publication-title: Environ. DNA doi: 10.1002/edn3.29 – start-page: 19 volume-title: The Conservation Status of Pelagic Sharks and Rays year: 2009 ident: 68843_CR3 – volume: 59 start-page: 508 year: 1996 ident: 68843_CR34 publication-title: Bull. Mar. Sci. – volume: 21 start-page: 2555 year: 2012 ident: 68843_CR9 publication-title: Mol. Ecol. doi: 10.1111/j.1365-294X.2012.05600.x – year: 2019 ident: 68843_CR51 publication-title: Environ. DNA doi: 10.1002/edn3.49 – volume: 5 start-page: e11968 year: 2010 ident: 68843_CR4 publication-title: PLoS One doi: 10.1371/journal.pone.0011968 – volume: 87 start-page: 845 year: 1989 ident: 68843_CR40 publication-title: Fish. Bull. – volume: 71 start-page: 135 year: 2004 ident: 68843_CR41 publication-title: Environ. Biol. Fish. doi: 10.1023/B:EBFI.0000045710.18997.f7 – volume: 8 start-page: 1 year: 2017 ident: 68843_CR53 publication-title: Front. Microbiol. doi: 10.3389/fmicb.2017.00108 – volume: 30 start-page: 109 year: 2016 ident: 68843_CR10 publication-title: Endanger. Species Res. doi: 10.3354/esr00731 – volume-title: A Field Guide to the Sharks of the World year: 2005 ident: 68843_CR35 – year: 2019 ident: 68843_CR55 publication-title: Conserv. Genet. Resour. doi: 10.1101/765321 – volume: 17 start-page: 221 year: 2017 ident: 68843_CR52 publication-title: Mol. Ecol. Resour. doi: 10.1111/1755-0998.12619 – volume: 16 start-page: 1401 year: 2016 ident: 68843_CR16 publication-title: Mol. Ecol. Resour. doi: 10.1111/1755-0998.12522 – start-page: 217 volume-title: Electronic Tagging and Tracking in Marine Fisheries year: 2001 ident: 68843_CR42 doi: 10.1007/978-94-017-1402-0_10 – volume: 19 start-page: 597 year: 2019 ident: 68843_CR31 publication-title: Mol. Ecol. Resour. doi: 10.1111/1755-0998.12987 – volume: 59 start-page: 624 year: 2002 ident: 68843_CR1 publication-title: Can. J. Fish. Aquat. Sci. doi: 10.1139/f02-036 – volume: 5 start-page: 127 year: 2013 ident: 68843_CR39 publication-title: Mar. Coast. Fish. doi: 10.1080/19425120.2012.758204 – volume: 5 start-page: e3044 year: 2017 ident: 68843_CR47 publication-title: PeerJ doi: 10.7717/peerj.3044 – volume: 5 start-page: 1 year: 2018 ident: 68843_CR11 publication-title: Front. Mar. Sci. doi: 10.3389/fmars.2018.00133
SSID	ssj0000529419
Score	2.4290116
Snippet	The integration of eDNA analysis into the population assessment and monitoring of sharks could greatly improve temporal and spatial data used for management...
SourceID	pubmedcentral proquest pubmed crossref springer
SourceType	Open Access Repository Aggregation Database Index Database Enrichment Source Publisher
StartPage	11847
SubjectTerms	631/158/2039 631/158/2452 631/158/672 Animals Aquatic habitats Bays Carcharhinus limbatus Carcharhinus melanopterus Environmental assessment Environmental DNA Environmental DNA - analysis Environmental DNA - genetics Environmental monitoring Female Fish populations Florida Humanities and Social Sciences Male multidisciplinary Polymerase chain reaction Population Dynamics Real-Time Polymerase Chain Reaction Reproduction - genetics Sampling Science Science (multidisciplinary) Seasonal variations Seasons Seawater - analysis Sharks Sharks - genetics Species Specificity Tropical Climate Water analysis Water sampling Water temperature
Title	Environmental DNA detection tracks established seasonal occurrence of blacktip sharks (Carcharhinus limbatus) in a semi-enclosed subtropical bay
URI	https://link.springer.com/article/10.1038/s41598-020-68843-0 https://www.ncbi.nlm.nih.gov/pubmed/32678294 https://www.proquest.com/docview/2424342491 https://www.proquest.com/docview/2424999196 https://pubmed.ncbi.nlm.nih.gov/PMC7367289
Volume	10
WOSCitedRecordID	wos000550057200160&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D
hasFullText	1
inHoldings	1
isFullTextHit
isPrint
journalDatabaseRights	– providerCode: PRVAON databaseName: DOAJ Directory of Open Access Journals customDbUrl: eissn: 2045-2322 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0000529419 issn: 2045-2322 databaseCode: DOA dateStart: 20110101 isFulltext: true titleUrlDefault: https://www.doaj.org/ providerName: Directory of Open Access Journals – providerCode: PRVHPJ databaseName: ROAD: Directory of Open Access Scholarly Resources customDbUrl: eissn: 2045-2322 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0000529419 issn: 2045-2322 databaseCode: M~E dateStart: 20110101 isFulltext: true titleUrlDefault: https://road.issn.org providerName: ISSN International Centre – providerCode: PRVPQU databaseName: Biological Science Database (ProQuest) customDbUrl: eissn: 2045-2322 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0000529419 issn: 2045-2322 databaseCode: M7P dateStart: 20110101 isFulltext: true titleUrlDefault: http://search.proquest.com/biologicalscijournals providerName: ProQuest – providerCode: PRVPQU databaseName: Health & Medical Collection customDbUrl: eissn: 2045-2322 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0000529419 issn: 2045-2322 databaseCode: 7X7 dateStart: 20110101 isFulltext: true titleUrlDefault: https://search.proquest.com/healthcomplete providerName: ProQuest – providerCode: PRVPQU databaseName: ProQuest Central customDbUrl: eissn: 2045-2322 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0000529419 issn: 2045-2322 databaseCode: BENPR dateStart: 20110101 isFulltext: true titleUrlDefault: https://www.proquest.com/central providerName: ProQuest – providerCode: PRVPQU databaseName: Publicly Available Content Database customDbUrl: eissn: 2045-2322 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0000529419 issn: 2045-2322 databaseCode: PIMPY dateStart: 20110101 isFulltext: true titleUrlDefault: http://search.proquest.com/publiccontent providerName: ProQuest – providerCode: PRVPQU databaseName: Science Database customDbUrl: eissn: 2045-2322 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0000529419 issn: 2045-2322 databaseCode: M2P dateStart: 20110101 isFulltext: true titleUrlDefault: https://search.proquest.com/sciencejournals providerName: ProQuest
link	http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV3db9MwED-xDSRe-IYVRmUkHkAQramT2H5CY3SCh1URAqk8RY7jqBFd0jUJ0v4L_mTunLRVmdgLD_GLP2Lrzr7z3fl3AK-55hkKamRe1K29YJxpT-V-7uUm81EccD8VXbIJMZ3K2UzFvcGt7sMq12eiO6izypCN_JieMXD8lP9heelR1ijyrvYpNPbggFASuAvdizc2FvJiBb7q38qMuDyuUV7RmzK6M0kZcG-0K4-uKZnXYyX_cpg6OXR2_39X8ADu9RooO-lY5iHcsuUjuNPlpLx6DL8n26dv2OzT9IRltnEBWyXDuZmfNcN_6i6aPmNkZCRtnlXGOKwnY1mVs5QMg02xZPVcr7DLm9MOlWlelG3NFsVFqpu2fsuKkmkc46LwsOeiqmnENm1W1ZK4h6X66gl8P5t8O_3s9WkbPBMK1Xjj0GQ2wIuKUaEKUhtyK3MRpYrbKJV-qAVh6GSoqmWhCUKt8FAxuY-ncmTJ18Ofwn5ZlfYQWCS5NCFXubV5gGylySlqLQ-UTKUYBwPw18RLTI9pTqk1FonzrXOZdARPkOCJI3gyGsC7TZ9lh-hxY-ujNTGTfnfXyZaSA3i1qcZ9Sc4WXdqq7dqQ8q2iATzrWGjzO1SZUTFTOH2xw1ybBoT5vVtTFnOH_S14JPCOPID3azbcTuvfq3h-8ypewN0xbQnCC42OYL9ZtfYl3Da_mqJeDWFPzIQr5RAOPk6m8dehM11geT6Oh27PYU385Tz-8QdJSjEg
linkProvider	ProQuest
linkToHtml	http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMw1V1Jb9QwFH4qBQQX9iVQwEgggSDqJM5iHxCquqhVy6iHIvWWOo6jiZgmwyQBzb_gl_AbeS_baKjorQcOc4rtiZ3vbX4bwBuueIKCGsGLurXtuYmyZeqkdqoTB8UBd-KwbTYRjsfi9FQer8HvPheGwip7ntgw6qTQdEe-SWkMHH_S-Tz7blPXKPKu9i00WlgcmsVPNNnKTwc7-H3fuu7e7sn2vt11FbC1H8rKdn2dGA_1aC196cXG50akYRBLboJYOL4KqcRLgppE4mvPVxIxr1MHmUZgyBXBcd1rcB3VCFc0oYLHw50Oec08R3a5OSMuNkuUj5TDRjaaEB63R6vy74JSezE28y8HbSP39u7-byd2D-50GjbbakniPqyZ_AHcbHtuLh7Cr91lah8O2xlvscRUTUBazvAs9LeS4R5Vmy2QMLpEJWuFFVo3tay0YUXKYrr4rLIZKydqjlPebbdVpyZZXpdsmp3HqqrL9yzLmcI1zjMbZ06Lklas42pezIg6WKwWj-DrlRzHY1jPi9w8BRYILrTPZWpM6iHZKHL6GsM9KWIRup4FTg-WSHc126l1yDRqYge4iFqARQiwqAFYNLLgwzBn1lYsuXT0Rg-eqONeZbREjgWvh8fId8iZpHJT1O0YMi5kYMGTFrLD36FJgIqnxNcPV8A8DKCa5qtP8mzS1DYPeRC6QlrwsYf98rX-vYtnl-_iFdzaP_lyFB0djA-fw22XyJFqowYbsF7Na_MCbugfVVbOXzb0zODsqsnhD8JbhFM
linkToPdf	http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMw1V1Jb9NAFH4qZREX9sVQYJBAAoGV2ONl5oBQ1TSiKopyAKk3dzweKxapHWIblH_B7-HX8Z6XRKGitx445OSZiWf8vW3eBvCKK56goEbwom5te26ibJk6qZ3qxEFxwJ04bJtNhJOJODmR0x343efCUFhlzxMbRp0Umu7IB5TGwPEnnUHahUVMR-OPi-82dZAiT2vfTqOFyLFZ_UTzrfxwNMJv_dp1x4dfDj7ZXYcBW_uhrGzX14nxUKfW0pdebHxuRBoGseQmiIXjq5DKvSSoVSS-9nwlEf86dZCBBIbcEhzXvQJXQypa3oQNTtf3O-RB8xzZ5ekMuRiUKCspn43sNSE8bg-3ZeE5Bfd8nOZfztpGBo5v_8-ndwdudZo3229J5S7smPweXG97ca7uw6_DTcofDhtN9lliqiZQLWd4LvpbyXC_qs0iSBhdrpIVwwqtmxpX2rAiZTFdiFbZgpUztcQpbw7aalSzLK9LNs_OYlXV5VuW5UzhGmeZjTPnRUkr1nG1LBZENSxWqwfw9VKO4yHs5kVuHgMLBBfa5zI1JvWQnBQ5g43hnhSxCF3PAqcHTqS7Wu7UUmQeNTEFXEQt2CIEW9SALRpa8G49Z9FWMrlw9F4PpKjjamW0QZEFL9ePkR-Rk0nlpqjbMWR0yMCCRy1813-HpgIqpBJfP9wC9noA1TrffpJns6bmeciD0BXSgvc9CWxe69-7eHLxLl7ADaSC6PPR5Pgp3HSJMqlkarAHu9WyNs_gmv5RZeXyeUPaDE4vmxr-AAqNjRA
openUrl	ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Environmental+DNA+detection+tracks+established+seasonal+occurrence+of+blacktip+sharks+%28Carcharhinus+limbatus%29+in+a+semi-enclosed+subtropical+bay&rft.jtitle=Scientific+reports&rft.au=Postaire%2C+Bautisse+D.&rft.au=Bakker%2C+Judith&rft.au=Gardiner%2C+Jayne&rft.au=Wiley%2C+Tonya+R.&rft.date=2020-07-16&rft.issn=2045-2322&rft.eissn=2045-2322&rft.volume=10&rft.issue=1&rft_id=info:doi/10.1038%2Fs41598-020-68843-0&rft.externalDBID=n%2Fa&rft.externalDocID=10_1038_s41598_020_68843_0
thumbnail_l	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=2045-2322&client=summon
thumbnail_m	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=2045-2322&client=summon
thumbnail_s	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=2045-2322&client=summon