Nanopore sensing at ultra-low concentrations using single-molecule dielectrophoretic trapping

Single-molecule techniques are being developed with the exciting prospect of revolutionizing the healthcare industry by generating vast amounts of genetic and proteomic data. One exceptionally promising route is in the use of nanopore sensors. However, a well-known complexity is that detection and c...

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Veröffentlicht in:Nature communications Jg. 7; H. 1; S. 10217 - 9
Hauptverfasser: Freedman, Kevin J., Otto, Lauren M., Ivanov, Aleksandar P., Barik, Avijit, Oh, Sang-Hyun, Edel, Joshua B.
Format: Journal Article
Sprache:Englisch
Veröffentlicht: London Nature Publishing Group UK 06.01.2016
Nature Publishing Group
Nature Portfolio
Schlagworte:
639/638/11
639/925/350/1058
9/10
9/74
Biosensing Techniques - instrumentation
Biosensing Techniques - methods
DNA - chemistry
DNA - genetics
Electrochemical Techniques
Electrodes
Experiments
Health care industry
Humanities and Social Sciences
Membranes, Artificial
multidisciplinary
Nanopores
Science
Science (multidisciplinary)
Trapping
United States > US
Minnesota
ISSN:2041-1723, 2041-1723
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Zusammenfassung:Single-molecule techniques are being developed with the exciting prospect of revolutionizing the healthcare industry by generating vast amounts of genetic and proteomic data. One exceptionally promising route is in the use of nanopore sensors. However, a well-known complexity is that detection and capture is predominantly diffusion limited. This problem is compounded when taking into account the capture volume of a nanopore, typically 10 8 –10 10 times smaller than the sample volume. To rectify this disproportionate ratio, we demonstrate a simple, yet powerful, method based on coupling single-molecule dielectrophoretic trapping to nanopore sensing. We show that DNA can be captured from a controllable, but typically much larger, volume and concentrated at the tip of a metallic nanopore. This enables the detection of single molecules at concentrations as low as 5 fM, which is approximately a 10 3 reduction in the limit of detection compared with existing methods, while still maintaining efficient throughput. Nanopore sensors have shown tremendous potential for biomolecule sensing, though the diffusion-controlled capture can limit the speed of analysis. Here, the authors report a dielectrophoretic method to concentrate DNA near the tip of a nanopore, reducing the limit of detection by three orders of magnitude.
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ISSN:2041-1723
2041-1723
DOI:10.1038/ncomms10217