Serum microRNAs as diagnostic markers of endometriosis: a comprehensive array-based analysis

To investigate serum microRNAs (miRNAs) in women with endometriosis. Case-control study. University hospital. Women with (n = 24) and without (n = 24) endometriosis. Serum samples were obtained from surgically diagnosed subjects. miRNA from women with without endometriosis were used for microarray p...

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Published in:Fertility and sterility Vol. 106; no. 2; pp. 402 - 409
Main Authors: Cosar, Emine, Mamillapalli, Ramanaiah, Ersoy, Gulcin Sahin, Cho, SihYun, Seifer, Benjamin, Taylor, Hugh S
Format: Journal Article
Language:English
Published: United States 01.08.2016
Subjects:
Adult
Area Under Curve
Case-Control Studies
Connecticut
Endometriosis - blood
Endometriosis - diagnosis
Endometriosis - genetics
Female
Gene Expression Profiling - methods
Genetic Markers
Genetic Predisposition to Disease
Hospitals, University
Humans
Logistic Models
MicroRNAs - blood
MicroRNAs - genetics
Oligonucleotide Array Sequence Analysis
Phenotype
Predictive Value of Tests
Real-Time Polymerase Chain Reaction
Reproducibility of Results
ROC Curve
Seoul
Endometriosis
miRNA
microarray
biomarker
serum
ISSN:1556-5653
Online Access:Get more information
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Summary:To investigate serum microRNAs (miRNAs) in women with endometriosis. Case-control study. University hospital. Women with (n = 24) and without (n = 24) endometriosis. Serum samples were obtained from surgically diagnosed subjects. miRNA from women with without endometriosis were used for microarray profiling and confirmed by means of quantitative real-time polymerase chain reaction (qRT-PCR). Receiver operating characteristic (ROC) analysis was performed on differentially expressed miRNAs. miR-3613-5p, miR-6755-3p were down-regulated and miR-125b-5p, miR-150-5p, miR-342-3p, miR-143-3p, miR-145-5p, miR-500a-3p, miR-451a, miR-18a-5p were up-regulated more than 10-fold in the microarray. These results were confirmed with the use of qRT-PCR. Among the differentially expressed miRNAs, miR-125b-5p expression levels had the highest area under the ROC curve (AUC). The maximum AUC score of 1.000 was achieved when combining miR-125b-5p, miR-451a, and miR-3613-5p with the use of a logistic regression model. We identified several miRNAs in serum that distinguished subjects with endometriosis from those without. miR-125b-5p had the greatest potential as a single diagnostic biomarker. A combination of that miRNA with miR-451a and miR-3613-5p further improved diagnostic performance.
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ISSN:1556-5653
DOI:10.1016/j.fertnstert.2016.04.013