Serum microRNAs as diagnostic markers of endometriosis: a comprehensive array-based analysis

To investigate serum microRNAs (miRNAs) in women with endometriosis. Case-control study. University hospital. Women with (n = 24) and without (n = 24) endometriosis. Serum samples were obtained from surgically diagnosed subjects. miRNA from women with without endometriosis were used for microarray p...

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Vydáno v:Fertility and sterility Ročník 106; číslo 2; s. 402 - 409
Hlavní autoři: Cosar, Emine, Mamillapalli, Ramanaiah, Ersoy, Gulcin Sahin, Cho, SihYun, Seifer, Benjamin, Taylor, Hugh S
Médium: Journal Article
Jazyk:angličtina
Vydáno: United States 01.08.2016
Témata:
Adult
Area Under Curve
Case-Control Studies
Connecticut
Endometriosis - blood
Endometriosis - diagnosis
Endometriosis - genetics
Female
Gene Expression Profiling - methods
Genetic Markers
Genetic Predisposition to Disease
Hospitals, University
Humans
Logistic Models
MicroRNAs - blood
MicroRNAs - genetics
Oligonucleotide Array Sequence Analysis
Phenotype
Predictive Value of Tests
Real-Time Polymerase Chain Reaction
Reproducibility of Results
ROC Curve
Seoul
Endometriosis
miRNA
microarray
biomarker
serum
ISSN:1556-5653
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Abstract To investigate serum microRNAs (miRNAs) in women with endometriosis. Case-control study. University hospital. Women with (n = 24) and without (n = 24) endometriosis. Serum samples were obtained from surgically diagnosed subjects. miRNA from women with without endometriosis were used for microarray profiling and confirmed by means of quantitative real-time polymerase chain reaction (qRT-PCR). Receiver operating characteristic (ROC) analysis was performed on differentially expressed miRNAs. miR-3613-5p, miR-6755-3p were down-regulated and miR-125b-5p, miR-150-5p, miR-342-3p, miR-143-3p, miR-145-5p, miR-500a-3p, miR-451a, miR-18a-5p were up-regulated more than 10-fold in the microarray. These results were confirmed with the use of qRT-PCR. Among the differentially expressed miRNAs, miR-125b-5p expression levels had the highest area under the ROC curve (AUC). The maximum AUC score of 1.000 was achieved when combining miR-125b-5p, miR-451a, and miR-3613-5p with the use of a logistic regression model. We identified several miRNAs in serum that distinguished subjects with endometriosis from those without. miR-125b-5p had the greatest potential as a single diagnostic biomarker. A combination of that miRNA with miR-451a and miR-3613-5p further improved diagnostic performance.
AbstractList OBJECTIVETo investigate serum microRNAs (miRNAs) in women with endometriosis.DESIGNCase-control study.SETTINGUniversity hospital.PATIENT(S)Women with (n = 24) and without (n = 24) endometriosis.INTERVENTION(S)Serum samples were obtained from surgically diagnosed subjects.MAIN OUTCOME MEASURE(S)miRNA from women with without endometriosis were used for microarray profiling and confirmed by means of quantitative real-time polymerase chain reaction (qRT-PCR). Receiver operating characteristic (ROC) analysis was performed on differentially expressed miRNAs.RESULT(S)miR-3613-5p, miR-6755-3p were down-regulated and miR-125b-5p, miR-150-5p, miR-342-3p, miR-143-3p, miR-145-5p, miR-500a-3p, miR-451a, miR-18a-5p were up-regulated more than 10-fold in the microarray. These results were confirmed with the use of qRT-PCR. Among the differentially expressed miRNAs, miR-125b-5p expression levels had the highest area under the ROC curve (AUC). The maximum AUC score of 1.000 was achieved when combining miR-125b-5p, miR-451a, and miR-3613-5p with the use of a logistic regression model.CONCLUSION(S)We identified several miRNAs in serum that distinguished subjects with endometriosis from those without. miR-125b-5p had the greatest potential as a single diagnostic biomarker. A combination of that miRNA with miR-451a and miR-3613-5p further improved diagnostic performance.
To investigate serum microRNAs (miRNAs) in women with endometriosis. Case-control study. University hospital. Women with (n = 24) and without (n = 24) endometriosis. Serum samples were obtained from surgically diagnosed subjects. miRNA from women with without endometriosis were used for microarray profiling and confirmed by means of quantitative real-time polymerase chain reaction (qRT-PCR). Receiver operating characteristic (ROC) analysis was performed on differentially expressed miRNAs. miR-3613-5p, miR-6755-3p were down-regulated and miR-125b-5p, miR-150-5p, miR-342-3p, miR-143-3p, miR-145-5p, miR-500a-3p, miR-451a, miR-18a-5p were up-regulated more than 10-fold in the microarray. These results were confirmed with the use of qRT-PCR. Among the differentially expressed miRNAs, miR-125b-5p expression levels had the highest area under the ROC curve (AUC). The maximum AUC score of 1.000 was achieved when combining miR-125b-5p, miR-451a, and miR-3613-5p with the use of a logistic regression model. We identified several miRNAs in serum that distinguished subjects with endometriosis from those without. miR-125b-5p had the greatest potential as a single diagnostic biomarker. A combination of that miRNA with miR-451a and miR-3613-5p further improved diagnostic performance.
Author Taylor, Hugh S
Mamillapalli, Ramanaiah
Cosar, Emine
Ersoy, Gulcin Sahin
Cho, SihYun
Seifer, Benjamin
Author_xml – sequence: 1
  givenname: Emine
  surname: Cosar
  fullname: Cosar, Emine
  organization: Department of Obstetrics, Gynecology, and Reproductive Sciences, Yale School of Medicine, New Haven, Connecticut
– sequence: 2
  givenname: Ramanaiah
  surname: Mamillapalli
  fullname: Mamillapalli, Ramanaiah
  email: ramana.mamillapalli@yale.edu
  organization: Department of Obstetrics, Gynecology, and Reproductive Sciences, Yale School of Medicine, New Haven, Connecticut. Electronic address: ramana.mamillapalli@yale.edu
– sequence: 3
  givenname: Gulcin Sahin
  surname: Ersoy
  fullname: Ersoy, Gulcin Sahin
  organization: Department of Obstetrics, Gynecology, and Reproductive Sciences, Yale School of Medicine, New Haven, Connecticut
– sequence: 4
  givenname: SihYun
  surname: Cho
  fullname: Cho, SihYun
  organization: Department of Obstetrics, Gynecology, and Reproductive Sciences, Yale School of Medicine, New Haven, Connecticut
– sequence: 5
  givenname: Benjamin
  surname: Seifer
  fullname: Seifer, Benjamin
  organization: Department of Obstetrics, Gynecology, and Reproductive Sciences, Yale School of Medicine, New Haven, Connecticut
– sequence: 6
  givenname: Hugh S
  surname: Taylor
  fullname: Taylor, Hugh S
  organization: Department of Obstetrics, Gynecology, and Reproductive Sciences, Yale School of Medicine, New Haven, Connecticut
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Keywords Endometriosis
miRNA
microarray
biomarker
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License Copyright © 2016 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.
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Snippet To investigate serum microRNAs (miRNAs) in women with endometriosis. Case-control study. University hospital. Women with (n = 24) and without (n = 24)...
OBJECTIVETo investigate serum microRNAs (miRNAs) in women with endometriosis.DESIGNCase-control study.SETTINGUniversity hospital.PATIENT(S)Women with (n = 24)...
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SubjectTerms Adult
Area Under Curve
Case-Control Studies
Connecticut
Endometriosis - blood
Endometriosis - diagnosis
Endometriosis - genetics
Female
Gene Expression Profiling - methods
Genetic Markers
Genetic Predisposition to Disease
Hospitals, University
Humans
Logistic Models
MicroRNAs - blood
MicroRNAs - genetics
Oligonucleotide Array Sequence Analysis
Phenotype
Predictive Value of Tests
Real-Time Polymerase Chain Reaction
Reproducibility of Results
ROC Curve
Seoul
Title Serum microRNAs as diagnostic markers of endometriosis: a comprehensive array-based analysis
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