Agar with embedded channels to study root growth

Agar have long been used as a growth media for plants. Here, we made agar media with embedded fluidic channels to study the effect of exposure to nutrient solution on root growth and pull-out force. Black Eye bean ( Vigna Unguiculata ) and Mung bean ( Vigna Radiata ) were used in this study due to t...

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Veröffentlicht in:Scientific reports Jg. 10; H. 1; S. 14231 - 12
Hauptverfasser: Aziz, Azlan Abdul, Lim, Kai Boon, Rahman, Ena Kartina Abdul, Nurmawati, Muhammad Hanafiah, Zuruzi, Abu Samah
Format: Journal Article
Sprache:Englisch
Veröffentlicht: London Nature Publishing Group UK 28.08.2020
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Abstract Agar have long been used as a growth media for plants. Here, we made agar media with embedded fluidic channels to study the effect of exposure to nutrient solution on root growth and pull-out force. Black Eye bean ( Vigna Unguiculata ) and Mung bean ( Vigna Radiata ) were used in this study due to their rapid root development. Agar media were fabricated using casting process with removable cores to form channels which were subsequently filled with nutrient solution. Upon germination, beans were transplanted onto the agar media and allowed to grow. Pull-out force was determined at 96, 120 and 144 h after germination by applying a force on the hypocotyl above the gel surface. The effect of nutrients was investigated by comparing corresponding data obtained from control plants which have not been exposed to nutrient solution. Pull-out force of Black Eye bean plantlets grown in agar with nutrient solution in channels was greater than those grown in gel without nutrients and was 110% greater after 144 h of germination. Pull-out force of Mung bean plantlets grown in agar with and without nutrient solution was similar. Tap root lengths of Black Eye bean and Mung Bean plantlets grown in agar with nutrient solution are shorter than those grown without nutrient. 
AbstractList Abstract Agar have long been used as a growth media for plants. Here, we made agar media with embedded fluidic channels to study the effect of exposure to nutrient solution on root growth and pull-out force. Black Eye bean (Vigna Unguiculata) and Mung bean (Vigna Radiata) were used in this study due to their rapid root development. Agar media were fabricated using casting process with removable cores to form channels which were subsequently filled with nutrient solution. Upon germination, beans were transplanted onto the agar media and allowed to grow. Pull-out force was determined at 96, 120 and 144 h after germination by applying a force on the hypocotyl above the gel surface. The effect of nutrients was investigated by comparing corresponding data obtained from control plants which have not been exposed to nutrient solution. Pull-out force of Black Eye bean plantlets grown in agar with nutrient solution in channels was greater than those grown in gel without nutrients and was 110% greater after 144 h of germination. Pull-out force of Mung bean plantlets grown in agar with and without nutrient solution was similar. Tap root lengths of Black Eye bean and Mung Bean plantlets grown in agar with nutrient solution are shorter than those grown without nutrient.
Agar have long been used as a growth media for plants. Here, we made agar media with embedded fluidic channels to study the effect of exposure to nutrient solution on root growth and pull-out force. Black Eye bean (Vigna Unguiculata) and Mung bean (Vigna Radiata) were used in this study due to their rapid root development. Agar media were fabricated using casting process with removable cores to form channels which were subsequently filled with nutrient solution. Upon germination, beans were transplanted onto the agar media and allowed to grow. Pull-out force was determined at 96, 120 and 144 h after germination by applying a force on the hypocotyl above the gel surface. The effect of nutrients was investigated by comparing corresponding data obtained from control plants which have not been exposed to nutrient solution. Pull-out force of Black Eye bean plantlets grown in agar with nutrient solution in channels was greater than those grown in gel without nutrients and was 110% greater after 144 h of germination. Pull-out force of Mung bean plantlets grown in agar with and without nutrient solution was similar. Tap root lengths of Black Eye bean and Mung Bean plantlets grown in agar with nutrient solution are shorter than those grown without nutrient.
Agar have long been used as a growth media for plants. Here, we made agar media with embedded fluidic channels to study the effect of exposure to nutrient solution on root growth and pull-out force. Black Eye bean ( Vigna Unguiculata ) and Mung bean ( Vigna Radiata ) were used in this study due to their rapid root development. Agar media were fabricated using casting process with removable cores to form channels which were subsequently filled with nutrient solution. Upon germination, beans were transplanted onto the agar media and allowed to grow. Pull-out force was determined at 96, 120 and 144 h after germination by applying a force on the hypocotyl above the gel surface. The effect of nutrients was investigated by comparing corresponding data obtained from control plants which have not been exposed to nutrient solution. Pull-out force of Black Eye bean plantlets grown in agar with nutrient solution in channels was greater than those grown in gel without nutrients and was 110% greater after 144 h of germination. Pull-out force of Mung bean plantlets grown in agar with and without nutrient solution was similar. Tap root lengths of Black Eye bean and Mung Bean plantlets grown in agar with nutrient solution are shorter than those grown without nutrient. 
Agar have long been used as a growth media for plants. Here, we made agar media with embedded fluidic channels to study the effect of exposure to nutrient solution on root growth and pull-out force. Black Eye bean (Vigna Unguiculata) and Mung bean (Vigna Radiata) were used in this study due to their rapid root development. Agar media were fabricated using casting process with removable cores to form channels which were subsequently filled with nutrient solution. Upon germination, beans were transplanted onto the agar media and allowed to grow. Pull-out force was determined at 96, 120 and 144 h after germination by applying a force on the hypocotyl above the gel surface. The effect of nutrients was investigated by comparing corresponding data obtained from control plants which have not been exposed to nutrient solution. Pull-out force of Black Eye bean plantlets grown in agar with nutrient solution in channels was greater than those grown in gel without nutrients and was 110% greater after 144 h of germination. Pull-out force of Mung bean plantlets grown in agar with and without nutrient solution was similar. Tap root lengths of Black Eye bean and Mung Bean plantlets grown in agar with nutrient solution are shorter than those grown without nutrient.Agar have long been used as a growth media for plants. Here, we made agar media with embedded fluidic channels to study the effect of exposure to nutrient solution on root growth and pull-out force. Black Eye bean (Vigna Unguiculata) and Mung bean (Vigna Radiata) were used in this study due to their rapid root development. Agar media were fabricated using casting process with removable cores to form channels which were subsequently filled with nutrient solution. Upon germination, beans were transplanted onto the agar media and allowed to grow. Pull-out force was determined at 96, 120 and 144 h after germination by applying a force on the hypocotyl above the gel surface. The effect of nutrients was investigated by comparing corresponding data obtained from control plants which have not been exposed to nutrient solution. Pull-out force of Black Eye bean plantlets grown in agar with nutrient solution in channels was greater than those grown in gel without nutrients and was 110% greater after 144 h of germination. Pull-out force of Mung bean plantlets grown in agar with and without nutrient solution was similar. Tap root lengths of Black Eye bean and Mung Bean plantlets grown in agar with nutrient solution are shorter than those grown without nutrient.
Agar have long been used as a growth media for plants. Here, we made agar media with embedded fluidic channels to study the effect of exposure to nutrient solution on root growth and pull-out force. Black Eye bean (Vigna Unguiculata) and Mung bean (Vigna Radiata) were used in this study due to their rapid root development. Agar media were fabricated using casting process with removable cores to form channels which were subsequently filled with nutrient solution. Upon germination, beans were transplanted onto the agar media and allowed to grow. Pull-out force was determined at 96, 120 and 144 h after germination by applying a force on the hypocotyl above the gel surface. The effect of nutrients was investigated by comparing corresponding data obtained from control plants which have not been exposed to nutrient solution. Pull-out force of Black Eye bean plantlets grown in agar with nutrient solution in channels was greater than those grown in gel without nutrients and was 110% greater after 144 h of germination. Pull-out force of Mung bean plantlets grown in agar with and without nutrient solution was similar. Tap root lengths of Black Eye bean and Mung Bean plantlets grown in agar with nutrient solution are shorter than those grown without nutrient. 
Agar have long been used as a growth media for plants. Here, we made agar media with embedded fluidic channels to study the effect of exposure to nutrient solution on root growth and pull-out force. Black Eye bean (Vigna Unguiculata) and Mung bean (Vigna Radiata) were used in this study due to their rapid root development. Agar media were fabricated using casting process with removable cores to form channels which were subsequently filled with nutrient solution. Upon germination, beans were transplanted onto the agar media and allowed to grow. Pull-out force was determined at 96, 120 and 144 h after germination by applying a force on the hypocotyl above the gel surface. The effect of nutrients was investigated by comparing corresponding data obtained from control plants which have not been exposed to nutrient solution. Pull-out force of Black Eye bean plantlets grown in agar with nutrient solution in channels was greater than those grown in gel without nutrients and was 110% greater after 144 h of germination. Pull-out force of Mung bean plantlets grown in agar with and without nutrient solution was similar. Tap root lengths of Black Eye bean and Mung Bean plantlets grown in agar with nutrient solution are shorter than those grown without nutrient.
ArticleNumber 14231
Author Aziz, Azlan Abdul
Lim, Kai Boon
Nurmawati, Muhammad Hanafiah
Rahman, Ena Kartina Abdul
Zuruzi, Abu Samah
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  givenname: Kai Boon
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  fullname: Rahman, Ena Kartina Abdul
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  givenname: Muhammad Hanafiah
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  fullname: Nurmawati, Muhammad Hanafiah
  organization: Singapore Accreditation Council
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  givenname: Abu Samah
  surname: Zuruzi
  fullname: Zuruzi, Abu Samah
  email: zsamah@alfaisal.edu
  organization: Department of Mechanical Engineering, College of Engineering, Alfaisal University
BackLink https://www.ncbi.nlm.nih.gov/pubmed/32859972$$D View this record in MEDLINE/PubMed
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YinZ-CWangY-LWangKA pH-responsive composite hydrogel beads based on agar and alginate for oral drug deliveryJ. Drug Deliv. Sci. Technol.20184312181:CAS:528:DC%2BC2sXhsFOgtbzE
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ArmisenRAThickening and Gelling Agents for Food1997BerlinSpringer
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CalbaHJaillardBFallavierPArvieuJ-CAgarose as a suitable substrate for use in the study of Al dynamics in the rhizospherePlant Soil199617867741:CAS:528:DyaK28Xhtlemtrw%3D
MurashigeTSkoogFA revised medium for rapid growth and bio assays with tobacco tissue culturesPhysiol. Plant.1962154734971:CAS:528:DyaF3sXksFKm
JocicSMestresGTenjeMFabrication of user-friendly and biomimetic 1,1′-carbonyldiimidazolecrosslinked gelatin/agar microfluidic devicesMater. Sci. Eng. C201776117511801:CAS:528:DC%2BC2sXls1Khu7o%3D
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BaiYLiuCHuangGLiWFengSA hyper-viscoelastic constitutive model for polyurea under uniaxial compressive loadingPolymers201681336432460
MaoBBentalebALoueratFDivouxTSnabrePHeat-induced aging of agar solutions: Impact on the structural and mechanical properties of agar gelsFood Hydrocolloids20176459691:CAS:528:DC%2BC28XhslKjsbzF
CervinoGFiorilloLHerfordASLainoLTroianoGAmorosoGCrimiSMatareseMD’AmicoCSiniscalchiENCicciùMAlginate materials and dental impression technique: a current state of the art and application to dental practiceMar. Drugs201917181:CAS:528:DC%2BC1MXhtFGmsLzP
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LK Wiersum (71076_CR41) 1958; 7
W Xu (71076_CR18) 2013; 3
Y Freile-Pelegrin (71076_CR31) 2007; 92
CA Schneider (71076_CR45) 2012; 9
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A Hodge (71076_CR44) 1992; 22
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SSID ssj0000529419
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Snippet Agar have long been used as a growth media for plants. Here, we made agar media with embedded fluidic channels to study the effect of exposure to nutrient...
Abstract Agar have long been used as a growth media for plants. Here, we made agar media with embedded fluidic channels to study the effect of exposure to...
SourceID doaj
pubmedcentral
proquest
pubmed
crossref
springer
SourceType Open Website
Open Access Repository
Aggregation Database
Index Database
Enrichment Source
Publisher
StartPage 14231
SubjectTerms 631/449/2653
639/166/988
639/301/923
639/301/930
639/638/298
Agar
Agar - pharmacology
Beans
Diffusion Chambers, Culture
Drug Delivery Systems - methods
Food
Germination
Germination - drug effects
Growth media
Humanities and Social Sciences
Hydrogels
Mechanical Phenomena
Mechanical properties
multidisciplinary
Nanomaterials
Nanoparticles
Nutrients
Nutrients - metabolism
Nutrients - pharmacology
Phytotoxicity
Plant growth
Plant Roots - growth & development
Plantlets
Root development
Science
Science (multidisciplinary)
Vigna - growth & development
Vigna radiata
Vigna unguiculata
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Title Agar with embedded channels to study root growth
URI https://link.springer.com/article/10.1038/s41598-020-71076-w
https://www.ncbi.nlm.nih.gov/pubmed/32859972
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Volume 10
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