Exploring antimicrobial resistance to beta-lactams, aminoglycosides and fluoroquinolones in E. coli and K. pneumoniae using proteogenomics

Antimicrobial resistance is mostly studied by means of phenotypic growth inhibition determinations, in combination with PCR confirmations or further characterization by means of whole genome sequencing (WGS). However, the actual proteins that cause resistance such as enzymes and a lack of porins can...

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Bibliographic Details
Published in:	Scientific reports Vol. 11; no. 1; pp. 12472 - 18
Main Authors:	Foudraine, Dimard E., Strepis, Nikolaos, Stingl, Christoph, ten Kate, Marian T., Verbon, Annelies, Klaassen, Corné H. W., Goessens, Wil H. F., Luider, Theo M., Dekker, Lennard J. M.
Format:	Journal Article
Language:	English
Published:	London Nature Publishing Group UK 14.06.2021 Nature Publishing Group Nature Portfolio
Subjects:	631/326/22/1434 631/337/475 Acetyltransferases - analysis Acetyltransferases - genetics Acetyltransferases - metabolism Amino Acid Sequence Aminoglycoside antibiotics Aminoglycosides - pharmacology Aminoglycosides - therapeutic use Anti-Bacterial Agents - therapeutic use Antibiotic resistance Antibiotics Antimicrobial agents Antimicrobial resistance Bacterial Proteins - analysis Bacterial Proteins - genetics Bacterial Proteins - metabolism beta-Lactamases - analysis beta-Lactamases - genetics beta-Lactamases - metabolism beta-Lactams - pharmacology beta-Lactams - therapeutic use Chromatography Chromatography, High Pressure Liquid - methods DNA, Bacterial - genetics DNA, Bacterial - isolation & purification Drug resistance Drug Resistance, Bacterial - genetics E coli Escherichia coli - drug effects Escherichia coli - enzymology Escherichia coli - genetics Escherichia coli - isolation & purification Fluoroquinolones Fluoroquinolones - pharmacology Fluoroquinolones - therapeutic use Gene Expression Regulation, Bacterial Genomes Humanities and Social Sciences Klebsiella pneumoniae - drug effects Klebsiella pneumoniae - enzymology Klebsiella pneumoniae - genetics Klebsiella pneumoniae - isolation & purification Liquid chromatography Mass spectrometry Mass spectroscopy Methyltransferases - analysis Methyltransferases - genetics Methyltransferases - metabolism multidisciplinary Phenotypes Porins Proteins Proteogenomics - methods Proteomes RNA, Ribosomal, 16S - metabolism rRNA 16S Science Science (multidisciplinary) Scientific imaging Tandem Mass Spectrometry - methods Whole Genome Sequencing β-Lactam antibiotics
ISSN:	2045-2322, 2045-2322
Online Access:	Get full text
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