Molecular characterization of Mycobacterium tuberculosis strains resistant to isoniazid

Abstract Objective/background Tuberculosis is a major public health problem and the emergence of drug resistance complicates the situation even more. It is therefore crucial to implement all conclusions from the studies that aim at a better understanding of the molecular mechanisms which govern the...

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Published in:International journal of mycobacteriology Vol. 5; no. 5; p. S151
Main Authors: Smaoui, Salma, Siala, Mariem, Hadj Fredj, Sondes, Kammoun, Sana, Marouane, Chema, Hachicha, Salma, Ghorbel, Asma, Gdoura, Radhouane, Slim, Leila, Ben Messaoud, Taieb, Messadi, Férièle
Format: Journal Article
Language:English
Published: Netherlands Elsevier Ltd 01.12.2016
Medknow Publications and Media Pvt. Ltd
Wolters Kluwer Medknow Publications
Subjects:
Drug resistance
Infectious Disease
Isoniazid
Medical Education
Mutation
Mycobacterium tuberculosis
Resistance
Tuberculosis
Resistance
Mutation
Mycobacterium tuberculosis
Isoniazid
ISSN:2212-5531, 2212-554X
Online Access:Get full text
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Summary:Abstract Objective/background Tuberculosis is a major public health problem and the emergence of drug resistance complicates the situation even more. It is therefore crucial to implement all conclusions from the studies that aim at a better understanding of the molecular mechanisms which govern the emergence and the evolution of drug resistance. The aim of this study is to assess the degree of involvement of the inhA and katG genes in the acquisition of isoniazid resistance in clinical strains of Mycobacterium tuberculosis. Methods The inhA and katG genes were sequenced in 21 strains of M. tuberculosis with different resistance profiles and from different regions. Results Analysis of the sequences obtained by comparison to those of the reference strain H37Rv showed that 95.2% had mutations. Kat G S315T was the most common mutation (85.7%). The mutation katG T275A was revealed in two strains (9.5%). Two different point mutations in the inhA gene and its promoter region were identified as C-15T and G56A at a frequency equal to 14% and 10%, respectively. The G56A mutation is a new silent mutation. Our study showed no correlation between found mutations and multidrug resistance. Among the 21 strains studied, only one strain showed no mutations. Conclusion In terms of this study, we characterized the mutations involved in resistance to isoniazid. katG S315T was by far the most frequent mutation, followed by C-15T. The frequency of these mutations was concordant with those reported in literature including those in intermediate tuberculosis endemic countries.
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ISSN:2212-5531
2212-554X
DOI:10.1016/j.ijmyco.2016.09.070