Use of a high platelet-to-RBC ratio of 2:1 is more effective in correcting trauma-induced coagulopathy than a ratio of 1:1 in a rat multiple trauma transfusion model

Background Platelet dysfunction importantly contributes to trauma-induced coagulopathy (TIC). Our aim was to examine the impact of transfusing platelets (PLTs) in a 2:1 PLT-to-red blood cell (RBC) ratio versus the standard 1:1 ratio on transfusion requirements, correction of TIC, and organ damage in...

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Vydané v:Intensive care medicine experimental Ročník 7; číslo Suppl 1; s. 42 - 12
Hlavní autori: Kleinveld, Derek J. B., Wirtz, Mathijs R., van den Brink, Daan P., Maas, M. Adrie W., Roelofs, Joris J. T. H., Goslings, J. Carel, Hollmann, Markus W., Juffermans, Nicole P.
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: Cham Springer International Publishing 25.07.2019
Springer Nature B.V
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Abstract Background Platelet dysfunction importantly contributes to trauma-induced coagulopathy (TIC). Our aim was to examine the impact of transfusing platelets (PLTs) in a 2:1 PLT-to-red blood cell (RBC) ratio versus the standard 1:1 ratio on transfusion requirements, correction of TIC, and organ damage in a rat multiple trauma transfusion model. Methods Mechanically ventilated male Sprague Dawley rats were traumatized by crush injury to the small intestine and liver and a fracture of the femur, followed by exsanguination until a mean arterial pressure (MAP) of 40 mmHg. Animals were randomly assigned to receive resuscitation in a high PLT dose (PLT to plasma to RBC in a ratio of 2:1:1) or a standard PLT dose (ratio of 1:1:1) until a MAP of 60 mmHg was reached ( n  = 8 per group). Blood samples were taken for biochemical and thromboelastometry (ROTEM) assessment. Organs were harvested for histopathology.Outcome measures were transfusion requirements needed to reach a pretargeted MAP, as well as ROTEM correction and organ failure. Results Trauma resulted in coagulopathy as assessed by deranged ROTEM results. Mortality rate was 19%, with all deaths occurring in the standard dose group. The severity of hypovolemic shock as assessed by lactate and base excess was not different in both groups. The volume of transfusion needed to reach the MAP target was lower in the high PLT dose group compared to the standard dose, albeit not statistically significant ( p  = 0.054). Transfusion with a high PLT dose resulted in significant stronger clot firmness compared to the standard dose at all time points following trauma, while platelet counts were similar. Organ failure as assessed by biochemical analysis and histopathology was not different between groups, nor were there any thromboembolic events recorded. Conclusions Resuscitation with a high (2:1) PLT-to-RBC ratio was more effective compared to standard (1:1) PLT-to-RBC ratio in treating TIC, with a trend towards reduced transfusion volumes. Also, high PLT dose did not aggravate organ damage. Transfusion strategies using higher PLT dose regiments might be a feasible treatment option in hemorrhaging trauma patients for the correction of TIC.
AbstractList Background Platelet dysfunction importantly contributes to trauma-induced coagulopathy (TIC). Our aim was to examine the impact of transfusing platelets (PLTs) in a 2:1 PLT-to-red blood cell (RBC) ratio versus the standard 1:1 ratio on transfusion requirements, correction of TIC, and organ damage in a rat multiple trauma transfusion model. Methods Mechanically ventilated male Sprague Dawley rats were traumatized by crush injury to the small intestine and liver and a fracture of the femur, followed by exsanguination until a mean arterial pressure (MAP) of 40 mmHg. Animals were randomly assigned to receive resuscitation in a high PLT dose (PLT to plasma to RBC in a ratio of 2:1:1) or a standard PLT dose (ratio of 1:1:1) until a MAP of 60 mmHg was reached ( n  = 8 per group). Blood samples were taken for biochemical and thromboelastometry (ROTEM) assessment. Organs were harvested for histopathology.Outcome measures were transfusion requirements needed to reach a pretargeted MAP, as well as ROTEM correction and organ failure. Results Trauma resulted in coagulopathy as assessed by deranged ROTEM results. Mortality rate was 19%, with all deaths occurring in the standard dose group. The severity of hypovolemic shock as assessed by lactate and base excess was not different in both groups. The volume of transfusion needed to reach the MAP target was lower in the high PLT dose group compared to the standard dose, albeit not statistically significant ( p  = 0.054). Transfusion with a high PLT dose resulted in significant stronger clot firmness compared to the standard dose at all time points following trauma, while platelet counts were similar. Organ failure as assessed by biochemical analysis and histopathology was not different between groups, nor were there any thromboembolic events recorded. Conclusions Resuscitation with a high (2:1) PLT-to-RBC ratio was more effective compared to standard (1:1) PLT-to-RBC ratio in treating TIC, with a trend towards reduced transfusion volumes. Also, high PLT dose did not aggravate organ damage. Transfusion strategies using higher PLT dose regiments might be a feasible treatment option in hemorrhaging trauma patients for the correction of TIC.
BackgroundPlatelet dysfunction importantly contributes to trauma-induced coagulopathy (TIC). Our aim was to examine the impact of transfusing platelets (PLTs) in a 2:1 PLT-to-red blood cell (RBC) ratio versus the standard 1:1 ratio on transfusion requirements, correction of TIC, and organ damage in a rat multiple trauma transfusion model.MethodsMechanically ventilated male Sprague Dawley rats were traumatized by crush injury to the small intestine and liver and a fracture of the femur, followed by exsanguination until a mean arterial pressure (MAP) of 40 mmHg. Animals were randomly assigned to receive resuscitation in a high PLT dose (PLT to plasma to RBC in a ratio of 2:1:1) or a standard PLT dose (ratio of 1:1:1) until a MAP of 60 mmHg was reached (n = 8 per group). Blood samples were taken for biochemical and thromboelastometry (ROTEM) assessment. Organs were harvested for histopathology.Outcome measures were transfusion requirements needed to reach a pretargeted MAP, as well as ROTEM correction and organ failure.ResultsTrauma resulted in coagulopathy as assessed by deranged ROTEM results. Mortality rate was 19%, with all deaths occurring in the standard dose group. The severity of hypovolemic shock as assessed by lactate and base excess was not different in both groups. The volume of transfusion needed to reach the MAP target was lower in the high PLT dose group compared to the standard dose, albeit not statistically significant (p = 0.054). Transfusion with a high PLT dose resulted in significant stronger clot firmness compared to the standard dose at all time points following trauma, while platelet counts were similar. Organ failure as assessed by biochemical analysis and histopathology was not different between groups, nor were there any thromboembolic events recorded.ConclusionsResuscitation with a high (2:1) PLT-to-RBC ratio was more effective compared to standard (1:1) PLT-to-RBC ratio in treating TIC, with a trend towards reduced transfusion volumes. Also, high PLT dose did not aggravate organ damage. Transfusion strategies using higher PLT dose regiments might be a feasible treatment option in hemorrhaging trauma patients for the correction of TIC.
Platelet dysfunction importantly contributes to trauma-induced coagulopathy (TIC). Our aim was to examine the impact of transfusing platelets (PLTs) in a 2:1 PLT-to-red blood cell (RBC) ratio versus the standard 1:1 ratio on transfusion requirements, correction of TIC, and organ damage in a rat multiple trauma transfusion model. Mechanically ventilated male Sprague Dawley rats were traumatized by crush injury to the small intestine and liver and a fracture of the femur, followed by exsanguination until a mean arterial pressure (MAP) of 40 mmHg. Animals were randomly assigned to receive resuscitation in a high PLT dose (PLT to plasma to RBC in a ratio of 2:1:1) or a standard PLT dose (ratio of 1:1:1) until a MAP of 60 mmHg was reached (n = 8 per group). Blood samples were taken for biochemical and thromboelastometry (ROTEM) assessment. Organs were harvested for histopathology.Outcome measures were transfusion requirements needed to reach a pretargeted MAP, as well as ROTEM correction and organ failure. Trauma resulted in coagulopathy as assessed by deranged ROTEM results. Mortality rate was 19%, with all deaths occurring in the standard dose group. The severity of hypovolemic shock as assessed by lactate and base excess was not different in both groups. The volume of transfusion needed to reach the MAP target was lower in the high PLT dose group compared to the standard dose, albeit not statistically significant (p = 0.054). Transfusion with a high PLT dose resulted in significant stronger clot firmness compared to the standard dose at all time points following trauma, while platelet counts were similar. Organ failure as assessed by biochemical analysis and histopathology was not different between groups, nor were there any thromboembolic events recorded. Resuscitation with a high (2:1) PLT-to-RBC ratio was more effective compared to standard (1:1) PLT-to-RBC ratio in treating TIC, with a trend towards reduced transfusion volumes. Also, high PLT dose did not aggravate organ damage. Transfusion strategies using higher PLT dose regiments might be a feasible treatment option in hemorrhaging trauma patients for the correction of TIC.
Abstract Background Platelet dysfunction importantly contributes to trauma-induced coagulopathy (TIC). Our aim was to examine the impact of transfusing platelets (PLTs) in a 2:1 PLT-to-red blood cell (RBC) ratio versus the standard 1:1 ratio on transfusion requirements, correction of TIC, and organ damage in a rat multiple trauma transfusion model. Methods Mechanically ventilated male Sprague Dawley rats were traumatized by crush injury to the small intestine and liver and a fracture of the femur, followed by exsanguination until a mean arterial pressure (MAP) of 40 mmHg. Animals were randomly assigned to receive resuscitation in a high PLT dose (PLT to plasma to RBC in a ratio of 2:1:1) or a standard PLT dose (ratio of 1:1:1) until a MAP of 60 mmHg was reached (n = 8 per group). Blood samples were taken for biochemical and thromboelastometry (ROTEM) assessment. Organs were harvested for histopathology.Outcome measures were transfusion requirements needed to reach a pretargeted MAP, as well as ROTEM correction and organ failure. Results Trauma resulted in coagulopathy as assessed by deranged ROTEM results. Mortality rate was 19%, with all deaths occurring in the standard dose group. The severity of hypovolemic shock as assessed by lactate and base excess was not different in both groups. The volume of transfusion needed to reach the MAP target was lower in the high PLT dose group compared to the standard dose, albeit not statistically significant (p = 0.054). Transfusion with a high PLT dose resulted in significant stronger clot firmness compared to the standard dose at all time points following trauma, while platelet counts were similar. Organ failure as assessed by biochemical analysis and histopathology was not different between groups, nor were there any thromboembolic events recorded. Conclusions Resuscitation with a high (2:1) PLT-to-RBC ratio was more effective compared to standard (1:1) PLT-to-RBC ratio in treating TIC, with a trend towards reduced transfusion volumes. Also, high PLT dose did not aggravate organ damage. Transfusion strategies using higher PLT dose regiments might be a feasible treatment option in hemorrhaging trauma patients for the correction of TIC.
Platelet dysfunction importantly contributes to trauma-induced coagulopathy (TIC). Our aim was to examine the impact of transfusing platelets (PLTs) in a 2:1 PLT-to-red blood cell (RBC) ratio versus the standard 1:1 ratio on transfusion requirements, correction of TIC, and organ damage in a rat multiple trauma transfusion model.BACKGROUNDPlatelet dysfunction importantly contributes to trauma-induced coagulopathy (TIC). Our aim was to examine the impact of transfusing platelets (PLTs) in a 2:1 PLT-to-red blood cell (RBC) ratio versus the standard 1:1 ratio on transfusion requirements, correction of TIC, and organ damage in a rat multiple trauma transfusion model.Mechanically ventilated male Sprague Dawley rats were traumatized by crush injury to the small intestine and liver and a fracture of the femur, followed by exsanguination until a mean arterial pressure (MAP) of 40 mmHg. Animals were randomly assigned to receive resuscitation in a high PLT dose (PLT to plasma to RBC in a ratio of 2:1:1) or a standard PLT dose (ratio of 1:1:1) until a MAP of 60 mmHg was reached (n = 8 per group). Blood samples were taken for biochemical and thromboelastometry (ROTEM) assessment. Organs were harvested for histopathology.Outcome measures were transfusion requirements needed to reach a pretargeted MAP, as well as ROTEM correction and organ failure.METHODSMechanically ventilated male Sprague Dawley rats were traumatized by crush injury to the small intestine and liver and a fracture of the femur, followed by exsanguination until a mean arterial pressure (MAP) of 40 mmHg. Animals were randomly assigned to receive resuscitation in a high PLT dose (PLT to plasma to RBC in a ratio of 2:1:1) or a standard PLT dose (ratio of 1:1:1) until a MAP of 60 mmHg was reached (n = 8 per group). Blood samples were taken for biochemical and thromboelastometry (ROTEM) assessment. Organs were harvested for histopathology.Outcome measures were transfusion requirements needed to reach a pretargeted MAP, as well as ROTEM correction and organ failure.Trauma resulted in coagulopathy as assessed by deranged ROTEM results. Mortality rate was 19%, with all deaths occurring in the standard dose group. The severity of hypovolemic shock as assessed by lactate and base excess was not different in both groups. The volume of transfusion needed to reach the MAP target was lower in the high PLT dose group compared to the standard dose, albeit not statistically significant (p = 0.054). Transfusion with a high PLT dose resulted in significant stronger clot firmness compared to the standard dose at all time points following trauma, while platelet counts were similar. Organ failure as assessed by biochemical analysis and histopathology was not different between groups, nor were there any thromboembolic events recorded.RESULTSTrauma resulted in coagulopathy as assessed by deranged ROTEM results. Mortality rate was 19%, with all deaths occurring in the standard dose group. The severity of hypovolemic shock as assessed by lactate and base excess was not different in both groups. The volume of transfusion needed to reach the MAP target was lower in the high PLT dose group compared to the standard dose, albeit not statistically significant (p = 0.054). Transfusion with a high PLT dose resulted in significant stronger clot firmness compared to the standard dose at all time points following trauma, while platelet counts were similar. Organ failure as assessed by biochemical analysis and histopathology was not different between groups, nor were there any thromboembolic events recorded.Resuscitation with a high (2:1) PLT-to-RBC ratio was more effective compared to standard (1:1) PLT-to-RBC ratio in treating TIC, with a trend towards reduced transfusion volumes. Also, high PLT dose did not aggravate organ damage. Transfusion strategies using higher PLT dose regiments might be a feasible treatment option in hemorrhaging trauma patients for the correction of TIC.CONCLUSIONSResuscitation with a high (2:1) PLT-to-RBC ratio was more effective compared to standard (1:1) PLT-to-RBC ratio in treating TIC, with a trend towards reduced transfusion volumes. Also, high PLT dose did not aggravate organ damage. Transfusion strategies using higher PLT dose regiments might be a feasible treatment option in hemorrhaging trauma patients for the correction of TIC.
ArticleNumber 42
Author Hollmann, Markus W.
Wirtz, Mathijs R.
Kleinveld, Derek J. B.
Roelofs, Joris J. T. H.
Juffermans, Nicole P.
Maas, M. Adrie W.
van den Brink, Daan P.
Goslings, J. Carel
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  organization: Department of Intensive Care Medicine, Amsterdam UMC
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  organization: Department of Intensive Care Medicine, Amsterdam UMC, Laboratory of Experimental Intensive Care and Anesthesiology, Amsterdam UMC
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Keywords Transfusion
Platelets
Trauma
Experimental
Coagulopathy
Language English
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springer_journals_10_1186_s40635_019_0242_5
PublicationCentury 2000
PublicationDate 2019-07-25
PublicationDateYYYYMMDD 2019-07-25
PublicationDate_xml – month: 07
  year: 2019
  text: 2019-07-25
  day: 25
PublicationDecade 2010
PublicationPlace Cham
PublicationPlace_xml – name: Cham
– name: Germany
– name: Heidelberg
PublicationTitle Intensive care medicine experimental
PublicationTitleAbbrev ICMx
PublicationTitleAlternate Intensive Care Med Exp
PublicationYear 2019
Publisher Springer International Publishing
Springer Nature B.V
SpringerOpen
Publisher_xml – name: Springer International Publishing
– name: Springer Nature B.V
– name: SpringerOpen
References TraversoLWLeeWPLangfordMJFluid resuscitation after an otherwise fatal hemorrhage: I. Crystalloid solutionsJ Trauma19862621681751:STN:280:DyaL287hsFentw%3D%3D308060210.1097/00005373-198602000-00014
RamseyMTFabianTCShahanCPSharpeJPMabrySEWeinbergJAA prospective study of platelet function in trauma patientsJ Trauma Acute Care Surg20168057267321:CAS:528:DC%2BC28Xmsl2qurs%3D2689508810.1097/TA.0000000000001017discussion 32-3
WirtzMathijs R.JurgensJordyZuurbierCoert J.RoelofsJoris J.T.H.SpinellaPhilip C.MuszynskiJennifer A.Carel GoslingsJ.JuffermansNicole P.Washing or filtering of blood products does not improve outcome in a rat model of trauma and multiple transfusionTransfusion20185911341453046102510.1111/trf.15039
EndoAShiraishiAFushimiKMurataKOtomoYOutcomes of patients receiving a massive transfusion for major traumaBr J Surg201810514261:STN:280:DC%2BB3c%2FnsVOltA%3D%3D2999951810.1002/bjs.10905
VogelSBodensteinRChenQFeilSFeilRRheinlaenderJPlatelet-derived HMGB1 is a critical mediator of thrombosisJ Clin Investig201512512463846542655168110.1172/JCI81660
ChenQVogelSLoughranPZuckerbraunBSBilliarTNealMDPlatelet derived HMGB1 regulates thrombosis and organ injury following traumaShock.2015432410.1097/SHK.0000000000000265
WuXSchwachaMGDubickMACapAPDarlingtonDNTrauma-related acute lung injury develops rapidly irrespective of resuscitation strategy in the ratShock.2016463S1081142717215010.1097/SHK.0000000000000652
HolcombJBWadeCEMichalekJEChisholmGBZarzabalLASchreiberMAIncreased plasma and platelet to red blood cell ratios improves outcome in 466 massively transfused civilian trauma patients. [Erratum appears in Ann Surg. 2011 Feb;253(2):392]Ann Surg2008248344745818791365
AnderssonUYangHHarrisHHigh-mobility group box 1 protein (HMGB1) operates as an alarmin outside as well as inside cellsSemin Immunol201838401:CAS:528:DC%2BC1cXkt1Knt7c%3D2953041010.1016/j.smim.2018.02.011
TuinmanPRSchultzMJJuffermansNPCoagulopathy as a therapeutic target for TRALI: rationale and possible sites of actionCurr Pharm Des20121822326732721:CAS:528:DC%2BC38XhtVGls7nO2262126910.2174/1381612811209023267
HolcombJBTilleyBCBaraniukSFoxEEWadeCEPodbielskiJMTransfusion of plasma, platelets, and red blood cells in a 1:1:1 vs a 1:1:2 ratio and mortality in patients with severe trauma: the PROPPR randomized clinical trialJAMA.201531354714821:CAS:528:DC%2BC2MXisVClsbs%3D25647203437474410.1001/jama.2015.12
HagemoJSChristiaansSCStanworthSJBrohiKJohanssonPIGoslingsJCDetection of acute traumatic coagulopathy and massive transfusion requirements by means of rotational thromboelastometry: an international prospective validation studyCrit Care2015199725888032437441110.1186/s13054-015-0823-y
Kasotakis G, Starr N, Nelson E, Sarkar B, Burke PA, Remick DG et al (2018) Platelet transfusion increases risk for acute respiratory distress syndrome in non-massively transfused blunt trauma patients. Eur J Trauma Emerg Surg
AyalaAWangPBaZFPerrinMMErtelWChaudryIHDifferential alterations in plasma IL-6 and TNF levels after trauma and hemorrhageAm J Phys19912601 Pt 2R167R1711:CAS:528:DyaK3MXht1SjtLs%3D
TariketSSutCHamzeh-CognasseHLaradiSGarraudOCognasseFPlatelet and TRALI: from blood component to organismTransfus Clin Biol2018252041:STN:280:DC%2BC1MjgtlKquw%3D%3D2963196310.1016/j.tracli.2018.03.006
CabreraCPMansonJShepherdJMTorranceHDWatsonDLonghiMPSignatures of inflammation and impending multiple organ dysfunction in the hyperacute phase of trauma: a prospective cohort studyPLoS Med2017147e100235228715416551340010.1371/journal.pmed.1002352
Dohan EhrenfestDMAndiaIZumsteinMAZhangCQPintoNRBieleckiTClassification of platelet concentrates (platelet-rich plasma-PRP, platelet-rich fibrin-PRF) for topical and infiltrative use in orthopedic and sports medicine: current consensus, clinical implications and perspectivesMuscles Ligaments Tendons J2014413924932440404964710.32098/mltj.01.2014.02
CastilloTNPouliotMAKimHJDragooJLComparison of growth factor and platelet concentration from commercial platelet-rich plasma separation systemsAm J Sports Med20113922662712105142810.1177/0363546510387517
MacLeodJBALynnMMcKenneyMGCohnSMMurthaMEarly coagulopathy predicts mortality in traumaJ Trauma200355139441285587910.1097/01.TA.0000075338.21177.EF
Naumann DN, Hazeldine J, Davies DJ, Bishop J, Midwinter MJ, Belli A et al (2017) Endotheliopathy of trauma is an on-scene phenomenon, and is associated with multiple organ dysfunction syndrome: a prospective observational study. PLoS One 12(12):e0189870.
SauaiaAMooreEEJohnsonJLChinTLBanerjeeASperryJLTemporal trends of postinjury multiple- organ failure: still resource intensive, morbid, and lethalJ Trauma Acute Care Surg201476358259224553523411608810.1097/TA.0000000000000147
CruzMVLukerJNCarneyBCBrummel-ZiedinsKEBravoMCOrfeoTReference ranges for rotational thromboelastometry in male Sprague Dawley ratsThromb J2017153129299031574709210.1186/s12959-017-0154-0
LeeCCLeeRPSubeqYMLeeCJChenTMHsuBGFluvastatin attenuates severe hemorrhagic shock-induced organ damage in ratsResuscitation.20098033723781:CAS:528:DC%2BD1MXhvFKjsb8%3D1915016610.1016/j.resuscitation.2008.12.003
VlaarAPHofstraJJKulikWvan LentheHNieuwlandRSchultzMJSupernatant of stored platelets causes lung inflammation and coagulopathy in a novel in vivo transfusion modelBlood.20101168136013681:CAS:528:DC%2BC3cXhtFersbfO2047928610.1182/blood-2009-10-248732
MorsingKSHPetersALvan BuulJDVlaarAPJThe role of endothelium in the onset of antibody-mediated TRALIBlood Rev2018321171:CAS:528:DC%2BC2sXhtlKmsrvK2882376310.1016/j.blre.2017.08.003
StettlerGRMooreEEMooreHBLawsonPJFragosoMNunnsGRThrombelastography indicates limitations of animal models of trauma-induced coagulopathyJ Surg Res201721720721228583756560336910.1016/j.jss.2017.05.027
MaegeleMSpinellaPCSchochlHThe acute coagulopathy of trauma: mechanisms and tools for risk stratificationShock.20123854504582304219210.1097/SHK.0b013e31826dbd23
TrowbridgeEAMartinJFSlaterDNKishkYTWarrenCWHarleyPJThe origin of platelet count and volumeClin Phys Physiol Meas1984531451701:STN:280:DyaL2M%2FisFyhsg%3D%3D648872210.1088/0143-0815/5/3/007
LetsonHLDobsonGPDifferential contributions of platelets and fibrinogen to early coagulopathy in a rat model of hemorrhagic shockThromb Res201614158651:CAS:528:DC%2BC28XktVCks7g%3D2697071410.1016/j.thromres.2016.03.007
BrownJBCohenMJMineiJPMaierRVWestMABilliarTRDebunking the survival bias myth: characterization of mortality during the initial 24 hours for patients requiring massive transfusionJ Trauma Acute Care Surg20127323583642284694010.1097/TA.0b013e31825889badiscussion 64
KutcherMERedickBJMcCreeryRCCraneIMGreenbergMDCacholaLMCharacterization of platelet dysfunction after traumaJ Trauma Acute Care Surg201273113191:CAS:528:DC%2BC38Xpsl2qtr8%3D22743367338738710.1097/TA.0b013e318256deab
VlaarAPHofstraJJLeviMKulikWNieuwlandRToolATSupernatant of aged erythrocytes causes lung inflammation and coagulopathy in a “two-hit” in vivo syngeneic transfusion modelAnesthesiology.20101131921032050849310.1097/ALN.0b013e3181de6f25
DarlingtonDWuXCapAPPolytrauma and hemorrhage affects platelet function in ratsTransfusion.20145420A21A
StraatMTuipAKleiTRLBeugerBMRoelofsJvan BruggenREndotoxemia results in trapping of transfused red blood cells in lungs with associated lung injuryShock.20174844844892891521810.1097/SHK.0000000000000875
GoodmanMDMakleyATHansemanDJPrittsTARobinsonBRAll the bang without the bucks: defining essential point-of-care testing for traumatic coagulopathyJ Trauma Acute Care Surg20157911171241:CAS:528:DC%2BC2MXhtValu7zI26091324555826410.1097/TA.0000000000000691discussion 24
LiYXiangMYuanYXiaoGZhangJJiangYHemorrhagic shock augments lung endothelial cell activation: role of temporal alterations of TLR4 and TLR2Am J Phys Regul Integr Comp Phys20092976R1670R16801:CAS:528:DC%2BD1MXhsFOltLfF
JuffermansNPVlaarAPPossible TRALI is a real entityTransfusion.20175710253925412869132110.1111/trf.14236
HolcombJBZarzabalLAMichalekJEKozarRASpinellaPCPerkinsJGIncreased platelet: RBC ratios are associated with improved survival after massive transfusionJ Trauma2011712 Suppl 3S318S3282181409910.1097/TA.0b013e318227edbb
MooreFAMcKinleyBAMooreEEThe next generation in shock resuscitationLancet.20043639425198819961519426010.1016/S0140-6736(04)16415-5
StanworthSJDavenportRCurryNSeeneyFEaglestoneSEdwardsAMortality from trauma haemorrhage and opportunities for improvement in transfusion practiceBr J Surg201610343573651:STN:280:DC%2BC28nnt12ntg%3D%3D2684172010.1002/bjs.10052
PeraltaRVijayAEl-MenyarAConsunjiRAfifiIMahmoodIEarly high ratio platelet transfusion in trauma resuscitation and its outcomesInt J Crit Illn Inj Sci20166418819328149824522576210.4103/2229-5151.195448
HL Letson (242_CR24) 2016; 141
JBA MacLeod (242_CR4) 2003; 55
M Maegele (242_CR8) 2012; 38
LW Traverso (242_CR9) 1986; 26
EA Trowbridge (242_CR40) 1984; 5
KSH Morsing (242_CR31) 2018; 32
R Peralta (242_CR35) 2016; 6
MT Ramsey (242_CR14) 2016; 80
X Wu (242_CR22) 2016; 46
Y Li (242_CR39) 2009; 297
PR Tuinman (242_CR36) 2012; 18
AP Vlaar (242_CR20) 2010; 113
JS Hagemo (242_CR26) 2015; 19
242_CR34
ME Kutcher (242_CR15) 2012; 73
CC Lee (242_CR28) 2009; 80
JB Holcomb (242_CR13) 2008; 248
Mathijs R. Wirtz (242_CR21) 2018; 59
MV Cruz (242_CR25) 2017; 15
M Straat (242_CR27) 2017; 48
JB Holcomb (242_CR12) 2011; 71
U Andersson (242_CR18) 2018; 38
FA Moore (242_CR10) 2004; 363
JB Holcomb (242_CR11) 2015; 313
TN Castillo (242_CR30) 2011; 39
NP Juffermans (242_CR32) 2017; 57
A Sauaia (242_CR5) 2014; 76
CP Cabrera (242_CR2) 2017; 14
JB Brown (242_CR6) 2012; 73
AP Vlaar (242_CR19) 2010; 116
GR Stettler (242_CR41) 2017; 217
242_CR1
A Endo (242_CR16) 2018; 105
DM Dohan Ehrenfest (242_CR29) 2014; 4
D Darlington (242_CR23) 2014; 54
S Vogel (242_CR38) 2015; 125
Q Chen (242_CR17) 2015; 43
A Ayala (242_CR37) 1991; 260
SJ Stanworth (242_CR3) 2016; 103
MD Goodman (242_CR7) 2015; 79
S Tariket (242_CR33) 2018; 25
References_xml – reference: RamseyMTFabianTCShahanCPSharpeJPMabrySEWeinbergJAA prospective study of platelet function in trauma patientsJ Trauma Acute Care Surg20168057267321:CAS:528:DC%2BC28Xmsl2qurs%3D2689508810.1097/TA.0000000000001017discussion 32-3
– reference: StraatMTuipAKleiTRLBeugerBMRoelofsJvan BruggenREndotoxemia results in trapping of transfused red blood cells in lungs with associated lung injuryShock.20174844844892891521810.1097/SHK.0000000000000875
– reference: AyalaAWangPBaZFPerrinMMErtelWChaudryIHDifferential alterations in plasma IL-6 and TNF levels after trauma and hemorrhageAm J Phys19912601 Pt 2R167R1711:CAS:528:DyaK3MXht1SjtLs%3D
– reference: VlaarAPHofstraJJKulikWvan LentheHNieuwlandRSchultzMJSupernatant of stored platelets causes lung inflammation and coagulopathy in a novel in vivo transfusion modelBlood.20101168136013681:CAS:528:DC%2BC3cXhtFersbfO2047928610.1182/blood-2009-10-248732
– reference: MacLeodJBALynnMMcKenneyMGCohnSMMurthaMEarly coagulopathy predicts mortality in traumaJ Trauma200355139441285587910.1097/01.TA.0000075338.21177.EF
– reference: MaegeleMSpinellaPCSchochlHThe acute coagulopathy of trauma: mechanisms and tools for risk stratificationShock.20123854504582304219210.1097/SHK.0b013e31826dbd23
– reference: KutcherMERedickBJMcCreeryRCCraneIMGreenbergMDCacholaLMCharacterization of platelet dysfunction after traumaJ Trauma Acute Care Surg201273113191:CAS:528:DC%2BC38Xpsl2qtr8%3D22743367338738710.1097/TA.0b013e318256deab
– reference: LiYXiangMYuanYXiaoGZhangJJiangYHemorrhagic shock augments lung endothelial cell activation: role of temporal alterations of TLR4 and TLR2Am J Phys Regul Integr Comp Phys20092976R1670R16801:CAS:528:DC%2BD1MXhsFOltLfF
– reference: DarlingtonDWuXCapAPPolytrauma and hemorrhage affects platelet function in ratsTransfusion.20145420A21A
– reference: PeraltaRVijayAEl-MenyarAConsunjiRAfifiIMahmoodIEarly high ratio platelet transfusion in trauma resuscitation and its outcomesInt J Crit Illn Inj Sci20166418819328149824522576210.4103/2229-5151.195448
– reference: TrowbridgeEAMartinJFSlaterDNKishkYTWarrenCWHarleyPJThe origin of platelet count and volumeClin Phys Physiol Meas1984531451701:STN:280:DyaL2M%2FisFyhsg%3D%3D648872210.1088/0143-0815/5/3/007
– reference: HagemoJSChristiaansSCStanworthSJBrohiKJohanssonPIGoslingsJCDetection of acute traumatic coagulopathy and massive transfusion requirements by means of rotational thromboelastometry: an international prospective validation studyCrit Care2015199725888032437441110.1186/s13054-015-0823-y
– reference: Kasotakis G, Starr N, Nelson E, Sarkar B, Burke PA, Remick DG et al (2018) Platelet transfusion increases risk for acute respiratory distress syndrome in non-massively transfused blunt trauma patients. Eur J Trauma Emerg Surg
– reference: VogelSBodensteinRChenQFeilSFeilRRheinlaenderJPlatelet-derived HMGB1 is a critical mediator of thrombosisJ Clin Investig201512512463846542655168110.1172/JCI81660
– reference: JuffermansNPVlaarAPPossible TRALI is a real entityTransfusion.20175710253925412869132110.1111/trf.14236
– reference: SauaiaAMooreEEJohnsonJLChinTLBanerjeeASperryJLTemporal trends of postinjury multiple- organ failure: still resource intensive, morbid, and lethalJ Trauma Acute Care Surg201476358259224553523411608810.1097/TA.0000000000000147
– reference: AnderssonUYangHHarrisHHigh-mobility group box 1 protein (HMGB1) operates as an alarmin outside as well as inside cellsSemin Immunol201838401:CAS:528:DC%2BC1cXkt1Knt7c%3D2953041010.1016/j.smim.2018.02.011
– reference: Dohan EhrenfestDMAndiaIZumsteinMAZhangCQPintoNRBieleckiTClassification of platelet concentrates (platelet-rich plasma-PRP, platelet-rich fibrin-PRF) for topical and infiltrative use in orthopedic and sports medicine: current consensus, clinical implications and perspectivesMuscles Ligaments Tendons J2014413924932440404964710.32098/mltj.01.2014.02
– reference: CabreraCPMansonJShepherdJMTorranceHDWatsonDLonghiMPSignatures of inflammation and impending multiple organ dysfunction in the hyperacute phase of trauma: a prospective cohort studyPLoS Med2017147e100235228715416551340010.1371/journal.pmed.1002352
– reference: WirtzMathijs R.JurgensJordyZuurbierCoert J.RoelofsJoris J.T.H.SpinellaPhilip C.MuszynskiJennifer A.Carel GoslingsJ.JuffermansNicole P.Washing or filtering of blood products does not improve outcome in a rat model of trauma and multiple transfusionTransfusion20185911341453046102510.1111/trf.15039
– reference: HolcombJBTilleyBCBaraniukSFoxEEWadeCEPodbielskiJMTransfusion of plasma, platelets, and red blood cells in a 1:1:1 vs a 1:1:2 ratio and mortality in patients with severe trauma: the PROPPR randomized clinical trialJAMA.201531354714821:CAS:528:DC%2BC2MXisVClsbs%3D25647203437474410.1001/jama.2015.12
– reference: WuXSchwachaMGDubickMACapAPDarlingtonDNTrauma-related acute lung injury develops rapidly irrespective of resuscitation strategy in the ratShock.2016463S1081142717215010.1097/SHK.0000000000000652
– reference: GoodmanMDMakleyATHansemanDJPrittsTARobinsonBRAll the bang without the bucks: defining essential point-of-care testing for traumatic coagulopathyJ Trauma Acute Care Surg20157911171241:CAS:528:DC%2BC2MXhtValu7zI26091324555826410.1097/TA.0000000000000691discussion 24
– reference: BrownJBCohenMJMineiJPMaierRVWestMABilliarTRDebunking the survival bias myth: characterization of mortality during the initial 24 hours for patients requiring massive transfusionJ Trauma Acute Care Surg20127323583642284694010.1097/TA.0b013e31825889badiscussion 64
– reference: HolcombJBZarzabalLAMichalekJEKozarRASpinellaPCPerkinsJGIncreased platelet: RBC ratios are associated with improved survival after massive transfusionJ Trauma2011712 Suppl 3S318S3282181409910.1097/TA.0b013e318227edbb
– reference: LeeCCLeeRPSubeqYMLeeCJChenTMHsuBGFluvastatin attenuates severe hemorrhagic shock-induced organ damage in ratsResuscitation.20098033723781:CAS:528:DC%2BD1MXhvFKjsb8%3D1915016610.1016/j.resuscitation.2008.12.003
– reference: StanworthSJDavenportRCurryNSeeneyFEaglestoneSEdwardsAMortality from trauma haemorrhage and opportunities for improvement in transfusion practiceBr J Surg201610343573651:STN:280:DC%2BC28nnt12ntg%3D%3D2684172010.1002/bjs.10052
– reference: TariketSSutCHamzeh-CognasseHLaradiSGarraudOCognasseFPlatelet and TRALI: from blood component to organismTransfus Clin Biol2018252041:STN:280:DC%2BC1MjgtlKquw%3D%3D2963196310.1016/j.tracli.2018.03.006
– reference: LetsonHLDobsonGPDifferential contributions of platelets and fibrinogen to early coagulopathy in a rat model of hemorrhagic shockThromb Res201614158651:CAS:528:DC%2BC28XktVCks7g%3D2697071410.1016/j.thromres.2016.03.007
– reference: StettlerGRMooreEEMooreHBLawsonPJFragosoMNunnsGRThrombelastography indicates limitations of animal models of trauma-induced coagulopathyJ Surg Res201721720721228583756560336910.1016/j.jss.2017.05.027
– reference: TraversoLWLeeWPLangfordMJFluid resuscitation after an otherwise fatal hemorrhage: I. Crystalloid solutionsJ Trauma19862621681751:STN:280:DyaL287hsFentw%3D%3D308060210.1097/00005373-198602000-00014
– reference: TuinmanPRSchultzMJJuffermansNPCoagulopathy as a therapeutic target for TRALI: rationale and possible sites of actionCurr Pharm Des20121822326732721:CAS:528:DC%2BC38XhtVGls7nO2262126910.2174/1381612811209023267
– reference: HolcombJBWadeCEMichalekJEChisholmGBZarzabalLASchreiberMAIncreased plasma and platelet to red blood cell ratios improves outcome in 466 massively transfused civilian trauma patients. [Erratum appears in Ann Surg. 2011 Feb;253(2):392]Ann Surg2008248344745818791365
– reference: Naumann DN, Hazeldine J, Davies DJ, Bishop J, Midwinter MJ, Belli A et al (2017) Endotheliopathy of trauma is an on-scene phenomenon, and is associated with multiple organ dysfunction syndrome: a prospective observational study. PLoS One 12(12):e0189870.
– reference: MooreFAMcKinleyBAMooreEEThe next generation in shock resuscitationLancet.20043639425198819961519426010.1016/S0140-6736(04)16415-5
– reference: ChenQVogelSLoughranPZuckerbraunBSBilliarTNealMDPlatelet derived HMGB1 regulates thrombosis and organ injury following traumaShock.2015432410.1097/SHK.0000000000000265
– reference: CruzMVLukerJNCarneyBCBrummel-ZiedinsKEBravoMCOrfeoTReference ranges for rotational thromboelastometry in male Sprague Dawley ratsThromb J2017153129299031574709210.1186/s12959-017-0154-0
– reference: EndoAShiraishiAFushimiKMurataKOtomoYOutcomes of patients receiving a massive transfusion for major traumaBr J Surg201810514261:STN:280:DC%2BB3c%2FnsVOltA%3D%3D2999951810.1002/bjs.10905
– reference: MorsingKSHPetersALvan BuulJDVlaarAPJThe role of endothelium in the onset of antibody-mediated TRALIBlood Rev2018321171:CAS:528:DC%2BC2sXhtlKmsrvK2882376310.1016/j.blre.2017.08.003
– reference: CastilloTNPouliotMAKimHJDragooJLComparison of growth factor and platelet concentration from commercial platelet-rich plasma separation systemsAm J Sports Med20113922662712105142810.1177/0363546510387517
– reference: VlaarAPHofstraJJLeviMKulikWNieuwlandRToolATSupernatant of aged erythrocytes causes lung inflammation and coagulopathy in a “two-hit” in vivo syngeneic transfusion modelAnesthesiology.20101131921032050849310.1097/ALN.0b013e3181de6f25
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Snippet Background Platelet dysfunction importantly contributes to trauma-induced coagulopathy (TIC). Our aim was to examine the impact of transfusing platelets (PLTs)...
Platelet dysfunction importantly contributes to trauma-induced coagulopathy (TIC). Our aim was to examine the impact of transfusing platelets (PLTs) in a 2:1...
BackgroundPlatelet dysfunction importantly contributes to trauma-induced coagulopathy (TIC). Our aim was to examine the impact of transfusing platelets (PLTs)...
Abstract Background Platelet dysfunction importantly contributes to trauma-induced coagulopathy (TIC). Our aim was to examine the impact of transfusing...
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StartPage 42
SubjectTerms Blood platelets
Blood transfusions
Coagulation
Coagulopathy
Critical Care Medicine
Experimental
Hemorrhage
Histopathology
Intensive
Intensive care
Medicine
Medicine & Public Health
Platelets
Rodents
Transfusion
Trauma
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Title Use of a high platelet-to-RBC ratio of 2:1 is more effective in correcting trauma-induced coagulopathy than a ratio of 1:1 in a rat multiple trauma transfusion model
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