A genetically encoded sensor for measuring serotonin dynamics

Serotonin (5-HT) is a phylogenetically conserved monoamine neurotransmitter modulating important processes in the brain. To directly visualize the release of 5-HT, we developed a genetically encoded G-protein-coupled receptor (GPCR)-activation-based 5-HT (GRAB 5-HT ) sensor with high sensitivity, hi...

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Vydáno v:	Nature neuroscience Ročník 24; číslo 5; s. 746 - 752
Hlavní autoři:	Wan, Jinxia, Peng, Wanling, Li, Xuelin, Qian, Tongrui, Song, Kun, Zeng, Jianzhi, Deng, Fei, Hao, Suyu, Feng, Jiesi, Zhang, Peng, Zhang, Yajun, Zou, Jing, Pan, Sunlei, Shin, Mimi, Venton, B. Jill, Zhu, J. Julius, Jing, Miao, Xu, Min, Li, Yulong
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	New York Nature Publishing Group US 01.05.2021 Nature Publishing Group
Témata:	631/1647/1888 631/378 631/443 96/35 Animal Genetics and Genomics Animals Behavioral Sciences Biological Techniques Biomedical and Life Sciences Biomedicine Biosensors Brain research Female G protein-coupled receptors Genetic code Genetic engineering Health aspects HEK293 Cells Humans Male Methods Mice Molecular dynamics Monoamines Neurobiology Neurons - metabolism Neurosciences Neurotransmitters Phylogeny Physiological aspects Rats Receptors, G-Protein-Coupled - metabolism Selectivity Sensitivity Sensors Serotonergic Neurons - metabolism Serotonin Serotonin - metabolism Signal Transduction - physiology technical-report China
ISSN:	1097-6256, 1546-1726, 1546-1726
On-line přístup:	Získat plný text
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Popis
Shrnutí:	Serotonin (5-HT) is a phylogenetically conserved monoamine neurotransmitter modulating important processes in the brain. To directly visualize the release of 5-HT, we developed a genetically encoded G-protein-coupled receptor (GPCR)-activation-based 5-HT (GRAB 5-HT ) sensor with high sensitivity, high selectivity, subsecond kinetics and subcellular resolution. GRAB 5-HT detects 5-HT release in multiple physiological and pathological conditions in both flies and mice and provides new insights into the dynamics and mechanisms of 5-HT signaling. The authors develop a genetically encoded GPCR-based sensor to image serotonin dynamics in behaving animals with high specificity, sensitivity and spatiotemporal resolution.
Bibliografie:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 Y.L. conceived and supervised the project. J.W., M.J., F.D., S.H., J.F., J. Zou, and S.P. performed the experiments related to developing, optimizing, and characterizing the sensor in cultured HEK293T cells and neurons. T.Q. performed the experiments using AAVs in slices. P.Z., Y.Z. and M.S. performed the simultaneous imaging and FSCV experiments using Sindbis virus in slices under the supervision of J.J.Z and B.J.V. X.L. and J. Zeng performed the Drosophila experiments. W.P. and K.S. performed the fiber-photometry recordings in behaving mice under the supervision of M.X. J.W. performed the two-photon imaging in head-fixed mice. All authors contributed to the data interpretation and analysis. Y.L. and J.W. wrote the manuscript with input from all other authors. Author contributions
ISSN:	1097-6256 1546-1726 1546-1726
DOI:	10.1038/s41593-021-00823-7