Actinidia chlorotic ringspot‐associated virus: a novel emaravirus infecting kiwifruit plants

Summary By integrating next‐generation sequencing (NGS), bioinformatics, electron microscopy and conventional molecular biology tools, a new virus infecting kiwifruit vines has been identified and characterized. Being associated with double‐membrane‐bound bodies in infected tissues and having a geno...

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Published in:Molecular plant pathology Vol. 18; no. 4; pp. 569 - 581
Main Authors: Zheng, Yazhou, Navarro, Beatriz, Wang, Guoping, Wang, Yanxiang, Yang, Zuokun, Xu, Wenxing, Zhu, Chenxi, Wang, Liping, Serio, Francesco Di, Hong, Ni
Format: Journal Article
Language:English
Published: England John Wiley & Sons, Inc 01.05.2017
John Wiley and Sons Inc
Subjects:
AcCRaV
Actinidia
Actinidia - virology
Base Sequence
Bioassays
biogenesis
Bioinformatics
Cell Membrane - metabolism
China
chlorotic ringspot
electron microscopy
Emaravirus
family Bunyaviridae
Gene Library
Genes
genome
Genome, Viral
genomics
genus Emaravirus
High-Throughput Nucleotide Sequencing
kiwifruit
leaves
Molecular biology
new species
Nicotiana - virology
Nicotiana benthamiana
nucleotides
open reading frames
Original
Phylogeny
Plant Diseases - virology
plant diseases and disorders
Plant Leaves - ultrastructure
Plant Leaves - virology
plant viruses
Plant Viruses - genetics
Plant Viruses - physiology
proteins
Reproducibility of Results
RNA
RNA interference
RNA, Viral - genetics
surveys
tissues
vines
viruses
China
chlorotic ringspot
family Bunyaviridae
Actinidia
AcCRaV
genus Emaravirus
ISSN:1464-6722, 1364-3703, 1364-3703
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Summary:Summary By integrating next‐generation sequencing (NGS), bioinformatics, electron microscopy and conventional molecular biology tools, a new virus infecting kiwifruit vines has been identified and characterized. Being associated with double‐membrane‐bound bodies in infected tissues and having a genome composed of RNA segments, each one containing a single open reading frame in negative polarity, this virus shows the typical features of members of the genus Emaravirus. Five genomic RNA segments were identified. Additional molecular signatures in the viral RNAs and in the proteins they encode, together with data from phylogenetic analyses, support the proposal of creating a new species in the genus Emaravirus to classify the novel virus, which is tentatively named Actinidia chlorotic ringspot‐associated virus (AcCRaV). Bioassays showed that AcCRaV is mechanically transmissible to Nicotiana benthamiana plants which, in turn, may develop chlorotic spots and ringspots. Field surveys disclosed the presence of AcCRaV in four different species of kiwifruit vines in five different provinces of central and western China, and support the association of the novel virus with symptoms of leaf chlorotic ringspots in Actinidia. Data on the molecular features of small RNAs of 21–24 nucleotides, derived from AcCRaV RNAs targeted by host RNA silencing mechanisms, are also reported, and possible molecular pathways involved in their biogenesis are discussed.
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ISSN:1464-6722
1364-3703
1364-3703
DOI:10.1111/mpp.12421