A 3D printed microfluidic device for production of functionalized hydrogel microcapsules for culture and differentiation of human Neuronal Stem Cells (hNSC)

We present here a microfluidic device that generates sub-millimetric hollow hydrogel spheres, encapsulating cells and coated internally with a layer of reconstituted extracellular matrix (ECM) of a few microns thick. The spherical capsules, composed of alginate hydrogel, originate from the spontaneo...

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Bibliographic Details
Published in:	Lab on a chip Vol. 16; no. 9; p. 1593
Main Authors:	Alessandri, Kevin, Feyeux, Maxime, Gurchenkov, Basile, Delgado, Christophe, Trushko, Anastasiya, Krause, Karl-Heinz, Vignjević, Daniela, Nassoy, Pierre, Roux, Aurélien
Format:	Journal Article
Language:	English
Published:	England 01.01.2016
Subjects:	Alginates - chemistry Automation, Laboratory Cell Culture Techniques - instrumentation Cell Line Cell Survival Cells, Immobilized - cytology Cells, Immobilized - metabolism Cellular Microenvironment Collagen - chemistry Drug Combinations Extracellular Matrix - chemistry Glucuronic Acid - chemistry Hexuronic Acids - chemistry High-Throughput Screening Assays - instrumentation Humans Hydrogels Induced Pluripotent Stem Cells - cytology Induced Pluripotent Stem Cells - metabolism Lab-On-A-Chip Devices Laminin - chemistry Nerve Tissue Proteins - metabolism Neural Stem Cells - cytology Neural Stem Cells - metabolism Neurogenesis Neurons - cytology Neurons - metabolism Printing, Three-Dimensional Proof of Concept Study Proteoglycans - chemistry Stem Cell Niche Surface Properties
ISSN:	1473-0189, 1473-0189
Online Access:	Get more information
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