A 3D printed microfluidic device for production of functionalized hydrogel microcapsules for culture and differentiation of human Neuronal Stem Cells (hNSC)

We present here a microfluidic device that generates sub-millimetric hollow hydrogel spheres, encapsulating cells and coated internally with a layer of reconstituted extracellular matrix (ECM) of a few microns thick. The spherical capsules, composed of alginate hydrogel, originate from the spontaneo...

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Vydáno v:Lab on a chip Ročník 16; číslo 9; s. 1593
Hlavní autoři: Alessandri, Kevin, Feyeux, Maxime, Gurchenkov, Basile, Delgado, Christophe, Trushko, Anastasiya, Krause, Karl-Heinz, Vignjević, Daniela, Nassoy, Pierre, Roux, Aurélien
Médium: Journal Article
Jazyk:angličtina
Vydáno: England 01.01.2016
Témata:
Alginates - chemistry
Automation, Laboratory
Cell Culture Techniques - instrumentation
Cell Line
Cell Survival
Cells, Immobilized - cytology
Cells, Immobilized - metabolism
Cellular Microenvironment
Collagen - chemistry
Drug Combinations
Extracellular Matrix - chemistry
Glucuronic Acid - chemistry
Hexuronic Acids - chemistry
High-Throughput Screening Assays - instrumentation
Humans
Hydrogels
Induced Pluripotent Stem Cells - cytology
Induced Pluripotent Stem Cells - metabolism
Lab-On-A-Chip Devices
Laminin - chemistry
Nerve Tissue Proteins - metabolism
Neural Stem Cells - cytology
Neural Stem Cells - metabolism
Neurogenesis
Neurons - cytology
Neurons - metabolism
Printing, Three-Dimensional
Proof of Concept Study
Proteoglycans - chemistry
Stem Cell Niche
Surface Properties
ISSN:1473-0189, 1473-0189
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Popis
Shrnutí:We present here a microfluidic device that generates sub-millimetric hollow hydrogel spheres, encapsulating cells and coated internally with a layer of reconstituted extracellular matrix (ECM) of a few microns thick. The spherical capsules, composed of alginate hydrogel, originate from the spontaneous instability of a multi-layered jet formed by co-extrusion using a coaxial flow device. We provide a simple design to manufacture this device using a DLP (digital light processing) 3D printer. Then, we demonstrate how the inner wall of the capsules can be decorated with a continuous ECM layer that is anchored to the alginate gel and mimics the basal membrane of a cellular niche. Finally, we used this approach to encapsulate human Neural Stem Cells (hNSC) derived from human Induced Pluripotent Stem Cells (hIPSC), which were further differentiated into neurons within the capsules with negligible loss of viability. Altogether, we show that these capsules may serve as cell micro-containers compatible with complex cell culture conditions and applications. These developments widen the field of research and biomedical applications of the cell encapsulation technology.
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ISSN:1473-0189
1473-0189
DOI:10.1039/c6lc00133e