A 3D printed microfluidic device for production of functionalized hydrogel microcapsules for culture and differentiation of human Neuronal Stem Cells (hNSC)
We present here a microfluidic device that generates sub-millimetric hollow hydrogel spheres, encapsulating cells and coated internally with a layer of reconstituted extracellular matrix (ECM) of a few microns thick. The spherical capsules, composed of alginate hydrogel, originate from the spontaneo...
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| Veröffentlicht in: | Lab on a chip Jg. 16; H. 9; S. 1593 |
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| Sprache: | Englisch |
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01.01.2016
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| Abstract | We present here a microfluidic device that generates sub-millimetric hollow hydrogel spheres, encapsulating cells and coated internally with a layer of reconstituted extracellular matrix (ECM) of a few microns thick. The spherical capsules, composed of alginate hydrogel, originate from the spontaneous instability of a multi-layered jet formed by co-extrusion using a coaxial flow device. We provide a simple design to manufacture this device using a DLP (digital light processing) 3D printer. Then, we demonstrate how the inner wall of the capsules can be decorated with a continuous ECM layer that is anchored to the alginate gel and mimics the basal membrane of a cellular niche. Finally, we used this approach to encapsulate human Neural Stem Cells (hNSC) derived from human Induced Pluripotent Stem Cells (hIPSC), which were further differentiated into neurons within the capsules with negligible loss of viability. Altogether, we show that these capsules may serve as cell micro-containers compatible with complex cell culture conditions and applications. These developments widen the field of research and biomedical applications of the cell encapsulation technology. |
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| AbstractList | We present here a microfluidic device that generates sub-millimetric hollow hydrogel spheres, encapsulating cells and coated internally with a layer of reconstituted extracellular matrix (ECM) of a few microns thick. The spherical capsules, composed of alginate hydrogel, originate from the spontaneous instability of a multi-layered jet formed by co-extrusion using a coaxial flow device. We provide a simple design to manufacture this device using a DLP (digital light processing) 3D printer. Then, we demonstrate how the inner wall of the capsules can be decorated with a continuous ECM layer that is anchored to the alginate gel and mimics the basal membrane of a cellular niche. Finally, we used this approach to encapsulate human Neural Stem Cells (hNSC) derived from human Induced Pluripotent Stem Cells (hIPSC), which were further differentiated into neurons within the capsules with negligible loss of viability. Altogether, we show that these capsules may serve as cell micro-containers compatible with complex cell culture conditions and applications. These developments widen the field of research and biomedical applications of the cell encapsulation technology. We present here a microfluidic device that generates sub-millimetric hollow hydrogel spheres, encapsulating cells and coated internally with a layer of reconstituted extracellular matrix (ECM) of a few microns thick. The spherical capsules, composed of alginate hydrogel, originate from the spontaneous instability of a multi-layered jet formed by co-extrusion using a coaxial flow device. We provide a simple design to manufacture this device using a DLP (digital light processing) 3D printer. Then, we demonstrate how the inner wall of the capsules can be decorated with a continuous ECM layer that is anchored to the alginate gel and mimics the basal membrane of a cellular niche. Finally, we used this approach to encapsulate human Neural Stem Cells (hNSC) derived from human Induced Pluripotent Stem Cells (hIPSC), which were further differentiated into neurons within the capsules with negligible loss of viability. Altogether, we show that these capsules may serve as cell micro-containers compatible with complex cell culture conditions and applications. These developments widen the field of research and biomedical applications of the cell encapsulation technology.We present here a microfluidic device that generates sub-millimetric hollow hydrogel spheres, encapsulating cells and coated internally with a layer of reconstituted extracellular matrix (ECM) of a few microns thick. The spherical capsules, composed of alginate hydrogel, originate from the spontaneous instability of a multi-layered jet formed by co-extrusion using a coaxial flow device. We provide a simple design to manufacture this device using a DLP (digital light processing) 3D printer. Then, we demonstrate how the inner wall of the capsules can be decorated with a continuous ECM layer that is anchored to the alginate gel and mimics the basal membrane of a cellular niche. Finally, we used this approach to encapsulate human Neural Stem Cells (hNSC) derived from human Induced Pluripotent Stem Cells (hIPSC), which were further differentiated into neurons within the capsules with negligible loss of viability. Altogether, we show that these capsules may serve as cell micro-containers compatible with complex cell culture conditions and applications. These developments widen the field of research and biomedical applications of the cell encapsulation technology. |
| Author | Feyeux, Maxime Delgado, Christophe Nassoy, Pierre Roux, Aurélien Krause, Karl-Heinz Alessandri, Kevin Trushko, Anastasiya Vignjević, Daniela Gurchenkov, Basile |
| Author_xml | – sequence: 1 givenname: Kevin surname: Alessandri fullname: Alessandri, Kevin email: aurelien.roux@unige.ch organization: University of Geneva, Department of Biochemistry, quai Ernest Ansermet 30, CH-1211 Geneva 4, Switzerland. aurelien.roux@unige.ch and Institut Curie et Centre National de la Recherche Scientifique, Unité Mixte de Recherche 168, F-75248 Paris, France and Université Pierre et Marie Curie, F-75005 Paris, France – sequence: 2 givenname: Maxime surname: Feyeux fullname: Feyeux, Maxime organization: Department of Pathology and Immunology, Faculty of Medicine, University of Geneva, CH-1211 Geneva 4, Switzerland – sequence: 3 givenname: Basile surname: Gurchenkov fullname: Gurchenkov, Basile organization: Institut Curie et Centre National de la Recherche Scientifique, Unité Mixte de Recherche 168, F-75248 Paris, France and Université Pierre et Marie Curie, F-75005 Paris, France and ICI, IGBMC, CNRS, UMR7104, F-67404 Illkirch-Graffenstaden, France and INSERM, U964, Université de Strasbourg, F-67400 Illkirch-Graffenstaden, France and Institut Curie et Centre National de la Recherche Scientifique, Unité Mixte de Recherche 144, F-75248 Paris, France – sequence: 4 givenname: Christophe surname: Delgado fullname: Delgado, Christophe organization: Department of Pathology and Immunology, Faculty of Medicine, University of Geneva, CH-1211 Geneva 4, Switzerland – sequence: 5 givenname: Anastasiya surname: Trushko fullname: Trushko, Anastasiya email: aurelien.roux@unige.ch organization: University of Geneva, Department of Biochemistry, quai Ernest Ansermet 30, CH-1211 Geneva 4, Switzerland. aurelien.roux@unige.ch – sequence: 6 givenname: Karl-Heinz surname: Krause fullname: Krause, Karl-Heinz organization: Department of Pathology and Immunology, Faculty of Medicine, University of Geneva, CH-1211 Geneva 4, Switzerland – sequence: 7 givenname: Daniela surname: Vignjević fullname: Vignjević, Daniela organization: Institut Curie et Centre National de la Recherche Scientifique, Unité Mixte de Recherche 144, F-75248 Paris, France – sequence: 8 givenname: Pierre surname: Nassoy fullname: Nassoy, Pierre email: aurelien.roux@unige.ch organization: University of Geneva, Department of Biochemistry, quai Ernest Ansermet 30, CH-1211 Geneva 4, Switzerland. aurelien.roux@unige.ch and Institut Curie et Centre National de la Recherche Scientifique, Unité Mixte de Recherche 168, F-75248 Paris, France and Université de Bordeaux, LP2N, UMR 5298, F-33400 Talence, France and Institut d'Optique & CNRS, LP2N, UMR 5298, F-33400 Talence, France – sequence: 9 givenname: Aurélien surname: Roux fullname: Roux, Aurélien email: aurelien.roux@unige.ch organization: University of Geneva, Department of Biochemistry, quai Ernest Ansermet 30, CH-1211 Geneva 4, Switzerland. aurelien.roux@unige.ch and Swiss National Centre for Competence in Research Programme Chemical Biology, 1211 Geneva, Switzerland |
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| SubjectTerms | Alginates - chemistry Automation, Laboratory Cell Culture Techniques - instrumentation Cell Line Cell Survival Cells, Immobilized - cytology Cells, Immobilized - metabolism Cellular Microenvironment Collagen - chemistry Drug Combinations Extracellular Matrix - chemistry Glucuronic Acid - chemistry Hexuronic Acids - chemistry High-Throughput Screening Assays - instrumentation Humans Hydrogels Induced Pluripotent Stem Cells - cytology Induced Pluripotent Stem Cells - metabolism Lab-On-A-Chip Devices Laminin - chemistry Nerve Tissue Proteins - metabolism Neural Stem Cells - cytology Neural Stem Cells - metabolism Neurogenesis Neurons - cytology Neurons - metabolism Printing, Three-Dimensional Proof of Concept Study Proteoglycans - chemistry Stem Cell Niche Surface Properties |
| Title | A 3D printed microfluidic device for production of functionalized hydrogel microcapsules for culture and differentiation of human Neuronal Stem Cells (hNSC) |
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