A Review on the Mechanism and Applications of CRISPR/Cas9/Cas12/Cas13/Cas14 Proteins Utilized for Genome Engineering

The clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated protein (CRISPR/Cas) system has altered life science research offering enormous options in manipulating, detecting, imaging, and annotating specific DNA or RNA sequences of diverse organisms. This system inc...

Celý popis

Uložené v:

Podrobná bibliografia
Vydané v:	Molecular biotechnology Ročník 65; číslo 3; s. 311 - 325
Hlavní autori:	Hillary, V. Edwin, Ceasar, S. Antony
Médium:	Journal Article
Jazyk:	English
Vydavateľské údaje:	New York Springer US 01.03.2023 Springer Nature B.V
Predmet:	Arrays Biochemistry Biological Techniques Biotechnology Cell Biology Chemistry Chemistry and Materials Science Coronaviruses COVID-19 CRISPR CRISPR-associated proteins CRISPR-Cas Systems Deoxyribonucleic acid DNA Gene Editing - methods Gene sequencing genome Genome editing Genomes gRNA Human Genetics Humans Nucleic acids Nucleotide sequence Protein Science Proteins researchers Review Paper Ribonucleic acid RNA SARS-CoV-2 - genetics Severe acute respiratory syndrome coronavirus 2 Spacers Viral diseases CRISPR DETECTR Genome engineering SHERLOCK Cas system
ISSN:	1073-6085, 1559-0305, 1559-0305
On-line prístup:	Získať plný text
Tagy:	Pridať tag Žiadne tagy, Buďte prvý, kto otaguje tento záznam!

Abstract	The clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated protein (CRISPR/Cas) system has altered life science research offering enormous options in manipulating, detecting, imaging, and annotating specific DNA or RNA sequences of diverse organisms. This system incorporates fragments of foreign DNA (spacers) into CRISPR cassettes, which are further transcribed into the CRISPR arrays and then processed to make guide RNA (gRNA). The CRISPR arrays are genes that encode Cas proteins. Cas proteins provide the enzymatic machinery required for acquiring new spacers targeting invading elements. Due to programmable sequence specificity, numerous Cas proteins such as Cas9, Cas12, Cas13, and Cas14 have been exploited to develop new tools for genome engineering. Cas variants stimulated genetic research and propelled the CRISPR/Cas tool for manipulating and editing nucleic acid sequences of living cells of diverse organisms. This review aims to provide detail on two classes (class 1 and 2) of the CRISPR/Cas system, and the mechanisms of all Cas proteins, including Cas12, Cas13, and Cas14 discovered so far. In addition, we also discuss the pros and cons and recent applications of various Cas proteins in diverse fields, including those used to detect viruses like severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). This review enables the researcher to gain knowledge on various Cas proteins and their applications, which have the potential to be used in next-generation precise genome engineering.
AbstractList	The clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated protein (CRISPR/Cas) system has altered life science research offering enormous options in manipulating, detecting, imaging, and annotating specific DNA or RNA sequences of diverse organisms. This system incorporates fragments of foreign DNA (spacers) into CRISPR cassettes, which are further transcribed into the CRISPR arrays and then processed to make guide RNA (gRNA). The CRISPR arrays are genes that encode Cas proteins. Cas proteins provide the enzymatic machinery required for acquiring new spacers targeting invading elements. Due to programmable sequence specificity, numerous Cas proteins such as Cas9, Cas12, Cas13, and Cas14 have been exploited to develop new tools for genome engineering. Cas variants stimulated genetic research and propelled the CRISPR/Cas tool for manipulating and editing nucleic acid sequences of living cells of diverse organisms. This review aims to provide detail on two classes (class 1 and 2) of the CRISPR/Cas system, and the mechanisms of all Cas proteins, including Cas12, Cas13, and Cas14 discovered so far. In addition, we also discuss the pros and cons and recent applications of various Cas proteins in diverse fields, including those used to detect viruses like severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). This review enables the researcher to gain knowledge on various Cas proteins and their applications, which have the potential to be used in next-generation precise genome engineering. The clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated protein (CRISPR/Cas) system has altered life science research offering enormous options in manipulating, detecting, imaging, and annotating specific DNA or RNA sequences of diverse organisms. This system incorporates fragments of foreign DNA (spacers) into CRISPR cassettes, which are further transcribed into the CRISPR arrays and then processed to make guide RNA (gRNA). The CRISPR arrays are genes that encode Cas proteins. Cas proteins provide the enzymatic machinery required for acquiring new spacers targeting invading elements. Due to programmable sequence specificity, numerous Cas proteins such as Cas9, Cas12, Cas13, and Cas14 have been exploited to develop new tools for genome engineering. Cas variants stimulated genetic research and propelled the CRISPR/Cas tool for manipulating and editing nucleic acid sequences of living cells of diverse organisms. This review aims to provide detail on two classes (class 1 and 2) of the CRISPR/Cas system, and the mechanisms of all Cas proteins, including Cas12, Cas13, and Cas14 discovered so far. In addition, we also discuss the pros and cons and recent applications of various Cas proteins in diverse fields, including those used to detect viruses like severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). This review enables the researcher to gain knowledge on various Cas proteins and their applications, which have the potential to be used in next-generation precise genome engineering.The clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated protein (CRISPR/Cas) system has altered life science research offering enormous options in manipulating, detecting, imaging, and annotating specific DNA or RNA sequences of diverse organisms. This system incorporates fragments of foreign DNA (spacers) into CRISPR cassettes, which are further transcribed into the CRISPR arrays and then processed to make guide RNA (gRNA). The CRISPR arrays are genes that encode Cas proteins. Cas proteins provide the enzymatic machinery required for acquiring new spacers targeting invading elements. Due to programmable sequence specificity, numerous Cas proteins such as Cas9, Cas12, Cas13, and Cas14 have been exploited to develop new tools for genome engineering. Cas variants stimulated genetic research and propelled the CRISPR/Cas tool for manipulating and editing nucleic acid sequences of living cells of diverse organisms. This review aims to provide detail on two classes (class 1 and 2) of the CRISPR/Cas system, and the mechanisms of all Cas proteins, including Cas12, Cas13, and Cas14 discovered so far. In addition, we also discuss the pros and cons and recent applications of various Cas proteins in diverse fields, including those used to detect viruses like severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). This review enables the researcher to gain knowledge on various Cas proteins and their applications, which have the potential to be used in next-generation precise genome engineering.
Author	Hillary, V. Edwin Ceasar, S. Antony
Author_xml	– sequence: 1 givenname: V. Edwin orcidid: 0000-0002-6208-5332 surname: Hillary fullname: Hillary, V. Edwin organization: Department of Biosciences, Rajagiri College of Social Sciences – sequence: 2 givenname: S. Antony orcidid: 0000-0003-4106-1531 surname: Ceasar fullname: Ceasar, S. Antony email: antony_sm2003@yahoo.co.in organization: Department of Biosciences, Rajagiri College of Social Sciences
BackLink	https://www.ncbi.nlm.nih.gov/pubmed/36163606$$D View this record in MEDLINE/PubMed
BookMark	eNqFkl9vFCEUxYmpsX_0C_hgSHzpy7QXGGB4Mdlsam1SY7PaZ0IZZpdmFkaYrdFPL7PbVu1DfbmQ8Dsn93LPIdoLMTiE3hI4IQDyNBMKjFVAaQXAhazgBTognKsKGPC9cgfJKgEN30eHOd8CUMJr9grtM0EEEyAO0DjDC3fn3Q8cAx5XDn92dmWCz2tsQotnw9B7a0YfQ8axw_PFxderxencZDUVQreVbWuNr1IcnS_k9eh7_8u1uIsJn7sQ1w6fhaUPziUflq_Ry8702b25P4_Q9cezb_NP1eWX84v57LKyXLKx6siN5C1YBkooaLk1jHJWAygjuBBUKVs31JbDyqZuCHcUTAukkKTjomZH6MPOd9jcrF1rXRiT6fWQ_Nqknzoar_99CX6ll_FOK06oElAMju8NUvy-cXnUa5-t63sTXNxkTZtGCqIE5_9HJWkEYw2RBX3_BL2NmxTKTxRKykIQOhm--7v5x64fdleAZgfYFHNOrtPWj9tVlVl8rwnoKSZ6FxNdYqK3MdHTXPSJ9MH9WRHbifIwLdGlP20_o_oNkGvLqw
CitedBy_id	crossref_primary_10_1016_j_ibmb_2025_104300 crossref_primary_10_3390_microorganisms13040927 crossref_primary_10_1016_j_actbio_2025_03_032 crossref_primary_10_1016_j_envres_2023_116573 crossref_primary_10_1016_j_jfueco_2025_100142 crossref_primary_10_3389_fgeed_2025_1533197 crossref_primary_10_1016_j_foohum_2025_100679 crossref_primary_10_3390_plants13141942 crossref_primary_10_1016_j_fertnstert_2023_02_040 crossref_primary_10_1016_j_aaf_2024_05_005 crossref_primary_10_3390_plants12233961 crossref_primary_10_1007_s10555_023_10149_4 crossref_primary_10_1007_s10068_023_01481_0 crossref_primary_10_1007_s00284_024_04024_w crossref_primary_10_1177_09760016241290651 crossref_primary_10_1038_s41596_024_01138_0 crossref_primary_10_1186_s12864_024_11073_9 crossref_primary_10_1007_s44372_025_00334_w crossref_primary_10_1002_cmdc_202300359 crossref_primary_10_1186_s12935_025_03846_3 crossref_primary_10_3390_cells13211794 crossref_primary_10_1016_j_trac_2025_118174 crossref_primary_10_1016_j_heliyon_2024_e34692 crossref_primary_10_21802_e_GMJ2025_A13 crossref_primary_10_1016_j_eti_2024_103625 crossref_primary_10_1016_j_microc_2025_115427 crossref_primary_10_1007_s10142_024_01421_z crossref_primary_10_3390_cells13141214 crossref_primary_10_1515_opag_2025_0458 crossref_primary_10_1016_j_fbr_2024_100366 crossref_primary_10_1016_j_snr_2025_100358 crossref_primary_10_1007_s10142_024_01314_1 crossref_primary_10_1002_biot_202300298 crossref_primary_10_1093_biolre_ioaf195 crossref_primary_10_1109_TMBMC_2025_3554671 crossref_primary_10_1002_ps_8597 crossref_primary_10_3390_insects15050339 crossref_primary_10_1038_s41417_024_00771_x crossref_primary_10_1270_jsbbs_23049 crossref_primary_10_1038_s41585_024_00901_y crossref_primary_10_1016_j_omtn_2025_102634 crossref_primary_10_11144_Javeriana_SC291_gewc crossref_primary_10_1016_j_pbiomolbio_2025_04_003 crossref_primary_10_1016_j_virol_2024_110160 crossref_primary_10_3389_fmicb_2024_1461227 crossref_primary_10_3389_ffgc_2025_1542459 crossref_primary_10_1089_crispr_2023_0058 crossref_primary_10_3390_plants13141884 crossref_primary_10_1007_s12672_025_03383_5 crossref_primary_10_1002_cyto_a_24815 crossref_primary_10_1007_s11105_025_01601_6 crossref_primary_10_1016_j_ijbiomac_2024_135065 crossref_primary_10_1016_j_cca_2024_119701 crossref_primary_10_1016_j_fbr_2024_100394 crossref_primary_10_1016_j_microc_2024_112624 crossref_primary_10_3390_microorganisms13010172 crossref_primary_10_1186_s12879_024_09451_5 crossref_primary_10_1016_j_microc_2024_111498 crossref_primary_10_1007_s00439_025_02757_7 crossref_primary_10_1016_j_bcab_2025_103528 crossref_primary_10_1016_j_visres_2023_108317 crossref_primary_10_1093_jambio_lxad114 crossref_primary_10_1186_s12864_024_11039_x crossref_primary_10_3390_biology12071037 crossref_primary_10_1016_j_exphem_2024_104669 crossref_primary_10_3390_jof9070719 crossref_primary_10_3390_agriculture15030350 crossref_primary_10_1016_j_aca_2024_343603 crossref_primary_10_3390_md23010039 crossref_primary_10_1186_s13059_024_03336_1 crossref_primary_10_1016_j_biotechadv_2025_108561 crossref_primary_10_3390_cells13070596 crossref_primary_10_1016_j_biopha_2023_115049 crossref_primary_10_3389_fmicb_2023_1186510 crossref_primary_10_1038_s12276_025_01434_z crossref_primary_10_1007_s12274_024_6748_5 crossref_primary_10_1039_D5BM00711A crossref_primary_10_12688_wellcomeopenres_23656_1 crossref_primary_10_1371_journal_pgen_1010930 crossref_primary_10_3389_fbioe_2023_1273988 crossref_primary_10_3390_microorganisms12051028 crossref_primary_10_1007_s00335_025_10143_x crossref_primary_10_1016_j_hlife_2024_06_002 crossref_primary_10_1016_j_ajps_2025_101068 crossref_primary_10_1186_s40364_024_00701_x crossref_primary_10_1016_j_fsi_2025_110220 crossref_primary_10_1016_j_ygeno_2025_111014 crossref_primary_10_15212_ZOONOSES_2023_0008 crossref_primary_10_1002_adtp_202400231 crossref_primary_10_1007_s11033_024_10205_4 crossref_primary_10_1089_hum_2023_115 crossref_primary_10_1186_s44330_024_00003_6 crossref_primary_10_1371_journal_pone_0325330 crossref_primary_10_38124_ijisrt_25mar736 crossref_primary_10_1016_j_mib_2024_102577 crossref_primary_10_35118_apjmbb_2025_033_3_07 crossref_primary_10_1016_j_synbio_2025_08_002 crossref_primary_10_1016_j_pbiomolbio_2024_09_001 crossref_primary_10_1007_s41060_025_00773_4 crossref_primary_10_3892_mmr_2023_13005 crossref_primary_10_3390_foods13203222 crossref_primary_10_1016_j_talanta_2025_128210 crossref_primary_10_1073_pnas_2414207122 crossref_primary_10_1016_j_omtn_2024_102370 crossref_primary_10_1016_j_aquaculture_2025_742355 crossref_primary_10_1016_j_cca_2024_120071 crossref_primary_10_1002_cbin_70082 crossref_primary_10_1016_j_trac_2025_118251 crossref_primary_10_1186_s12929_024_01070_1 crossref_primary_10_3390_plants14131983 crossref_primary_10_1016_j_pmpp_2024_102402 crossref_primary_10_3390_bios13090885 crossref_primary_10_1093_jxb_erad175 crossref_primary_10_1002_advs_202414918 crossref_primary_10_1016_j_snr_2025_100318 crossref_primary_10_1016_j_trac_2024_117902 crossref_primary_10_1016_j_ijbiomac_2025_147825 crossref_primary_10_1021_acs_molpharmaceut_4c00925 crossref_primary_10_1016_j_scienta_2025_113957 crossref_primary_10_1007_s11033_024_09424_6 crossref_primary_10_21926_obm_genet_2502289 crossref_primary_10_29105_agricolis_v1i2_11 crossref_primary_10_1038_s41598_025_99999_2 crossref_primary_10_1016_j_diagmicrobio_2024_116252 crossref_primary_10_3390_genes14040850 crossref_primary_10_3390_pharmaceutics16091197 crossref_primary_10_3390_plants13162269 crossref_primary_10_1186_s12943_024_02222_5 crossref_primary_10_3389_fbioe_2023_1327498 crossref_primary_10_3389_fimmu_2024_1354825 crossref_primary_10_1111_febs_17368 crossref_primary_10_1016_j_trecan_2025_08_001 crossref_primary_10_1038_s44320_024_00067_0 crossref_primary_10_1016_j_fbio_2025_106553 crossref_primary_10_1007_s11240_025_03195_z crossref_primary_10_1016_j_stress_2024_100578 crossref_primary_10_1007_s11033_023_08521_2 crossref_primary_10_1002_cjoc_202401325 crossref_primary_10_1016_j_mattod_2024_09_017 crossref_primary_10_1007_s00425_023_04199_9 crossref_primary_10_1038_s41598_024_78647_1 crossref_primary_10_3389_fgeed_2023_1272687 crossref_primary_10_1038_s41598_025_15463_1 crossref_primary_10_3390_microorganisms11102404 crossref_primary_10_3389_fgeed_2025_1611040 crossref_primary_10_3390_bios15060379 crossref_primary_10_1002_adhm_202500192 crossref_primary_10_1016_j_pmpp_2025_102640 crossref_primary_10_1007_s00344_025_11829_x crossref_primary_10_3390_v16091401 crossref_primary_10_1007_s00299_024_03346_0 crossref_primary_10_1021_acsomega_4c11282 crossref_primary_10_1016_j_trac_2025_118397 crossref_primary_10_1016_j_aquaculture_2023_740440 crossref_primary_10_1016_j_critrevonc_2025_104916
Cites_doi	10.1038/nmeth.2681 10.1002/bies.202200032 10.1046/j.1365-2958.2002.02839.x 10.1371/journal.pcbi.0010060 10.1021/acsnano.0c08165 10.1038/celldisc.2015.7 10.1093/gbe/evx192 10.1128/jb.169.12.5429-5433.1987 10.1038/ncomms1937 10.1016/B978-0-12-818140-9.00018-0 10.1021/acssynbio.1c00107 10.1128/iai.59.8.2695-2705.1991 10.1038/s41596-020-0302-z 10.1016/j.csbj.2020.08.031 10.1016/j.bbamcr.2016.06.009 10.1126/science.aar6245 10.1038/s41579-019-0299-x 10.1038/nrg1619 10.1080/19768354.2020.1726462 10.1093/nar/gks216 10.1126/science.aaq0179 10.1046/j.1365-2958.2000.01838.x 10.1016/j.cell.2016.11.053 10.1186/1745-6150-4-42 10.1038/s41586-020-2007-4 10.1016/j.biotechadv.2022.107963 10.1016/j.cell.2015.09.038 10.1038/nature09886 10.1016/j.cell.2016.12.031 10.1016/j.str.2009.03.019 10.1126/science.1225829 10.1038/nature19802 10.1038/nrmicro2577 10.1126/science.aav4294 10.1126/science.1138140 10.1016/j.febslet.2010.07.057 10.1126/science.1165771 10.1248/bpb.b18-00222 10.1016/j.cell.2017.11.032 10.1073/pnas.1104144108 10.1038/nbt.2170 10.1007/s00239-004-0046-3 10.1016/j.cell.2016.04.003 10.1186/s13059-019-1620-8 10.1021/acs.analchem.6b04630 10.1016/j.scib.2020.06.014 10.1016/j.molcel.2016.12.023 10.1038/nature21059 10.1099/mic.0.28048-0 10.1126/science.aas8836 10.1111/j.1365-2958.2010.07465.x 10.1038/nature17945 10.1038/ncomms14500 10.1038/s41596-019-0210-2 10.2174/1874070701913010173 10.1016/j.mib.2017.05.008 10.1038/nature13579 10.1186/s13059-018-1586-y 10.1016/j.biotechadv.2019.03.016 10.1021/acssensors.0c02365 10.1038/nsmb.3289 10.1016/bs.pmbts.2020.12.017 10.1038/nrg908 10.1016/j.biotechadv.2017.11.008 10.1128/jb.171.6.3553-3556.1989 10.1126/science.1179555 10.1128/JB.01415-07 10.1126/science.aam9321 10.1080/14737159.2021.1970535 10.1093/genetics/122.3.519 10.1016/j.cell.2014.02.001 10.3389/fpls.2020.584151 10.1016/j.bios.2020.112068 10.1101/2020.02.26.967026 10.1017/S0033583505004063 10.1038/nmeth.1542 10.1126/science.1231143 10.1126/science.1159689 10.1038/s41587-020-0513-4 10.1016/j.cell.2018.02.033 10.1038/nature24049 10.1016/j.tree.2008.06.005 10.1016/j.biopha.2020.110738
ContentType	Journal Article
Copyright	The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2022 Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. 2022. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature. Copyright Springer Nature B.V. Mar 2023 The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2022, Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.
Copyright_xml	– notice: The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2022 Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. – notice: 2022. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature. – notice: Copyright Springer Nature B.V. Mar 2023 – notice: The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2022, Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.
DBID	AAYXX CITATION CGR CUY CVF ECM EIF NPM 3V. 7QL 7QO 7T7 7U9 7X7 7XB 88A 88E 88I 8AO 8FD 8FE 8FG 8FH 8FI 8FJ 8FK ABJCF ABUWG AEUYN AFKRA AZQEC BBNVY BENPR BGLVJ BHPHI C1K CCPQU DWQXO FR3 FYUFA GHDGH GNUQQ H94 HCIFZ K9. L6V LK8 M0S M1P M2P M7N M7P M7S P64 PHGZM PHGZT PJZUB PKEHL PPXIY PQEST PQGLB PQQKQ PQUKI PRINS PTHSS Q9U 7X8 7S9 L.6 5PM
DOI	10.1007/s12033-022-00567-0
DatabaseName	CrossRef Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed ProQuest Central (Corporate) Bacteriology Abstracts (Microbiology B) Biotechnology Research Abstracts Industrial and Applied Microbiology Abstracts (Microbiology A) Virology and AIDS Abstracts Health & Medical Collection ProQuest Central (purchase pre-March 2016) Biology Database (Alumni Edition) Medical Database (Alumni Edition) Science Database (Alumni Edition) ProQuest Pharma Collection Technology Research Database ProQuest SciTech Collection ProQuest Technology Collection ProQuest Natural Science Collection Hospital Premium Collection Hospital Premium Collection (Alumni Edition) ProQuest Central (Alumni) (purchase pre-March 2016) Materials Science & Engineering Collection ProQuest Central (Alumni) ProQuest One Sustainability ProQuest Central UK/Ireland ProQuest Central Essentials - QC Biological Science Collection AUTh Library subscriptions: ProQuest Central Technology collection Natural Science Collection Environmental Sciences and Pollution Management ProQuest One Community College ProQuest Central Engineering Research Database Health Research Premium Collection Health Research Premium Collection (Alumni) ProQuest Central Student AIDS and Cancer Research Abstracts SciTech Premium Collection ProQuest Health & Medical Complete (Alumni) ProQuest Engineering Collection Biological Sciences Health & Medical Collection (Alumni) Medical Database Science Database Algology Mycology and Protozoology Abstracts (Microbiology C) Biological Science Database Engineering Database Biotechnology and BioEngineering Abstracts Proquest Central Premium ProQuest One Academic (New) ProQuest Health & Medical Research Collection ProQuest One Academic Middle East (New) ProQuest One Health & Nursing ProQuest One Academic Eastern Edition (DO NOT USE) ProQuest One Applied & Life Sciences ProQuest One Academic (retired) ProQuest One Academic UKI Edition ProQuest Central China Engineering collection ProQuest Central Basic MEDLINE - Academic AGRICOLA AGRICOLA - Academic PubMed Central (Full Participant titles)
DatabaseTitle	CrossRef MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) ProQuest Central Student ProQuest Central Essentials SciTech Premium Collection ProQuest Central China Environmental Sciences and Pollution Management ProQuest One Applied & Life Sciences ProQuest One Sustainability Health Research Premium Collection Natural Science Collection Health & Medical Research Collection Biological Science Collection Industrial and Applied Microbiology Abstracts (Microbiology A) ProQuest Central (New) ProQuest Medical Library (Alumni) Engineering Collection Engineering Database Virology and AIDS Abstracts ProQuest Science Journals (Alumni Edition) ProQuest Biological Science Collection ProQuest One Academic Eastern Edition ProQuest Hospital Collection ProQuest Technology Collection Health Research Premium Collection (Alumni) Biological Science Database ProQuest Hospital Collection (Alumni) Biotechnology and BioEngineering Abstracts ProQuest Health & Medical Complete ProQuest One Academic UKI Edition Engineering Research Database ProQuest One Academic ProQuest One Academic (New) Technology Collection Technology Research Database ProQuest One Academic Middle East (New) ProQuest Health & Medical Complete (Alumni) ProQuest Central (Alumni Edition) ProQuest One Community College ProQuest One Health & Nursing ProQuest Natural Science Collection ProQuest Pharma Collection ProQuest Biology Journals (Alumni Edition) ProQuest Central ProQuest Health & Medical Research Collection ProQuest Engineering Collection Biotechnology Research Abstracts Health and Medicine Complete (Alumni Edition) ProQuest Central Korea Bacteriology Abstracts (Microbiology B) Algology Mycology and Protozoology Abstracts (Microbiology C) AIDS and Cancer Research Abstracts ProQuest Central Basic ProQuest Science Journals ProQuest SciTech Collection ProQuest Medical Library Materials Science & Engineering Collection ProQuest Central (Alumni) MEDLINE - Academic AGRICOLA AGRICOLA - Academic
DatabaseTitleList	ProQuest Central Student AGRICOLA MEDLINE - Academic MEDLINE
Database_xml	– sequence: 1 dbid: NPM name: PubMed url: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: BENPR name: ProQuest Central url: https://www.proquest.com/central sourceTypes: Aggregation Database
DeliveryMethod	fulltext_linktorsrc
Discipline	Engineering Chemistry Biology
EISSN	1559-0305
EndPage	325
ExternalDocumentID	PMC9512960 36163606 10_1007_s12033_022_00567_0
Genre	Journal Article Review
GroupedDBID	--- -Y2 .86 .VR 06C 06D 0R~ 0VY 123 1N0 2.D 203 28- 29M 2J2 2JN 2JY 2KG 2KM 2LR 2VQ 2~H 30V 3SX 4.4 406 408 40D 40E 53G 5VS 67N 6NX 7X7 88E 88I 8AO 8FE 8FG 8FH 8FI 8FJ 8TC 8UJ 95- 95. 95~ 96X A8Z AAAVM AABHQ AACDK AAHBH AAHNG AAIAL AAIKT AAJBT AAJKR AANXM AANZL AAPKM AARHV AARTL AASML AATNV AATVU AAUYE AAWCG AAYIU AAYQN AAYTO AAYZH ABAKF ABBRH ABDBE ABDZT ABECU ABFTV ABHLI ABHQN ABJCF ABJNI ABJOX ABKCH ABMNI ABMQK ABNWP ABQBU ABQSL ABSXP ABTEG ABTHY ABTKH ABTMW ABUWG ABWNU ABXPI ACAOD ACBXY ACCUX ACDTI ACGFS ACGOD ACHSB ACHXU ACIWK ACKNC ACMDZ ACMLO ACOKC ACOMO ACPIV ACPRK ACZOJ ADBBV ADHHG ADHIR ADHKG ADKNI ADKPE ADMLS ADRFC ADTPH ADURQ ADYFF ADZKW AEBTG AEFQL AEGAL AEGNC AEJHL AEJRE AEKMD AEMSY AENEX AEOHA AEPYU AESKC AETLH AEUYN AEVLU AEXYK AFBBN AFDZB AFEXP AFGCZ AFKRA AFLOW AFQWF AFRAH AFWTZ AFZKB AGAYW AGDGC AGGDS AGJBK AGMZJ AGQEE AGQMX AGQPQ AGRTI AGWIL AGWZB AGYKE AHAVH AHBYD AHKAY AHMBA AHPBZ AHSBF AIAKS AIGIU AIIXL AILAN AITGF AJBLW AJRNO AJZVZ ALIPV ALMA_UNASSIGNED_HOLDINGS ALWAN AMKLP AMXSW AMYLF AMYQR AOCGG ARMRJ ASPBG AVWKF AXYYD AYFIA AYJHY AZFZN AZQEC B-. BA0 BBNVY BBWZM BDATZ BENPR BGLVJ BGNMA BHPHI BPHCQ BVXVI CAG CCPQU COF CS3 CSCUP DDRTE DNIVK DPUIP DU5 DWQXO EBD EBLON EBS EIOEI EJD EMOBN ESBYG F5P FEDTE FERAY FFXSO FIGPU FINBP FNLPD FRRFC FSGXE FWDCC FYUFA G-Y G-Z GGCAI GGRSB GJIRD GNUQQ GNWQR GQ7 H13 HCIFZ HF~ HG6 HMCUK HMJXF HRMNR HVGLF HZ~ I-F IJ- IKXTQ ITM IWAJR IZQ I~X I~Z J-C J0Z JBSCW JZLTJ KOV L6V LK8 LLZTM M1P M2P M4Y M7P M7S MA- ML0 N2Q NB0 NDZJH NF0 NPVJJ NQJWS NU0 O9- O93 O9G O9I O9J OVD P19 P2P PF0 PHGZT PQQKQ PROAC PSQYO PT4 PT5 PTHSS Q2X QOK QOR QOS R4E R89 R9I RHV RIG RNI ROL RPX RSV RZK S16 S1Z S26 S27 S28 S3A S3B SAP SBL SCG SCLPG SDH SDM SHX SISQX SJYHP SNE SNPRN SNX SOHCF SOJ SPISZ SRMVM SSLCW STPWE SV3 SZN T13 T16 TEORI TSG TSV TUC TUS U2A U9L UG4 UKHRP UOJIU UTJUX UZXMN VC2 VFIZW W48 WK6 WK8 YLTOR ZMTXR ~EX ~KM AAYXX ABFSG ABRTQ ACSTC AEZWR AFFHD AFHIU AFOHR AHWEU AIXLP ATHPR CITATION PHGZM PJZUB PPXIY PQGLB CGR CUY CVF ECM EIF NPM 3V. 7QL 7QO 7T7 7U9 7XB 88A 8FD 8FK C1K ESTFP FR3 H94 K9. M7N P64 PKEHL PQEST PQUKI PRINS Q9U 7X8 PUEGO 7S9 L.6 5PM
ID	FETCH-LOGICAL-c573t-f1b75d0c309690d5ca32534009a6566299c482c99cc784815e20ad01d5c1f5643
IEDL.DBID	RSV
ISICitedReferencesCount	175
ISICitedReferencesURI	http://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=Summon&SrcAuth=ProQuest&DestLinkType=CitingArticles&DestApp=WOS_CPL&KeyUT=000860374300001&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D
ISSN	1073-6085 1559-0305
IngestDate	Tue Nov 04 02:07:07 EST 2025 Mon Sep 08 15:04:50 EDT 2025 Sun Sep 28 09:13:07 EDT 2025 Wed Nov 05 03:11:00 EST 2025 Thu Apr 03 07:07:04 EDT 2025 Tue Nov 18 22:35:46 EST 2025 Sat Nov 29 08:01:31 EST 2025 Thu Apr 10 07:57:32 EDT 2025
IsDoiOpenAccess	true
IsOpenAccess	true
IsPeerReviewed	true
IsScholarly	true
Issue	3
Keywords	CRISPR DETECTR Genome engineering SHERLOCK Cas system
Language	English
License	2022. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature. This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
LinkModel	DirectLink
MergedId	FETCHMERGED-LOGICAL-c573t-f1b75d0c309690d5ca32534009a6566299c482c99cc784815e20ad01d5c1f5643
Notes	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 ObjectType-Review-3 content type line 23
ORCID	0000-0003-4106-1531 0000-0002-6208-5332
OpenAccessLink	https://pubmed.ncbi.nlm.nih.gov/PMC9512960
PMID	36163606
PQID	2777173125
PQPubID	55363
PageCount	15
ParticipantIDs	pubmedcentral_primary_oai_pubmedcentral_nih_gov_9512960 proquest_miscellaneous_2887619655 proquest_miscellaneous_2718633817 proquest_journals_2777173125 pubmed_primary_36163606 crossref_citationtrail_10_1007_s12033_022_00567_0 crossref_primary_10_1007_s12033_022_00567_0 springer_journals_10_1007_s12033_022_00567_0
PublicationCentury	2000
PublicationDate	2023-03-01
PublicationDateYYYYMMDD	2023-03-01
PublicationDate_xml	– month: 03 year: 2023 text: 2023-03-01 day: 01
PublicationDecade	2020
PublicationPlace	New York
PublicationPlace_xml	– name: New York – name: United States – name: Totowa
PublicationTitle	Molecular biotechnology
PublicationTitleAbbrev	Mol Biotechnol
PublicationTitleAlternate	Mol Biotechnol
PublicationYear	2023
Publisher	Springer US Springer Nature B.V
Publisher_xml	– name: Springer US – name: Springer Nature B.V
References	VE Hillary (567_CR67) 2022; 44 I Yosef (567_CR44) 2012; 40 JP Broughton (567_CR60) 2020; 38 R Barrangou (567_CR28) 2007; 315 B Wiedenheft (567_CR41) 2009; 17 N Rudin (567_CR3) 1989; 122 B Al-Shayeb (567_CR69) 2020; 578 D Burstein (567_CR84) 2017; 542 F Hille (567_CR10) 2018; 172 LB Harrington (567_CR40) 2018; 362 C Anders (567_CR47) 2014; 513 JS Gootenberg (567_CR73) 2018; 360 Q He (567_CR59) 2020; 154 A Bandyopadhyay (567_CR64) 2020; 11 L Liu (567_CR71) 2017; 168 LA Marraffini (567_CR24) 2008; 322 KS Makarova (567_CR58) 2020; 18 MJ Kellner (567_CR76) 2020; 15 M Jinek (567_CR8) 2012; 337 S Konermann (567_CR74) 2018; 173 D Reyon (567_CR7) 2012; 30 F Palaz (567_CR82) 2021; 10 JD Sander (567_CR6) 2011; 8 KS Makarova (567_CR12) 2011; 9 AA Smargon (567_CR75) 2017; 65 A Mahas (567_CR86) 2018; 36 H Nishimasu (567_CR46) 2014; 156 PW Hermans (567_CR16) 1991; 59 H Lee (567_CR52) 2020; 24 MJ Kellner (567_CR77) 2019; 14 FJM Mojica (567_CR17) 2000; 36 MR Capecchi (567_CR1) 2005; 6 E Deltcheva (567_CR27) 2011; 471 A East-Seletsky (567_CR70) 2016; 538 567_CR61 SA Ceasar (567_CR9) 2016; 1863 P Horvath (567_CR29) 2010; 327 KA Datsenko (567_CR42) 2012; 3 S Rauch (567_CR81) 2020; 11 F Wang (567_CR13) 2019; 37 SA Shabalina (567_CR22) 2008; 23 H Yang (567_CR38) 2016; 167 T Tian (567_CR78) 2020; 15 HC Metsky (567_CR80) 2020 EV Koonin (567_CR21) 2017; 9 VE Hillary (567_CR68) 2021; 21 C Myhrvold (567_CR79) 2018; 360 MT McManus (567_CR2) 2002; 3 M Feng (567_CR62) 2020; 65 M Babu (567_CR43) 2011; 79 VE Hillary (567_CR54) 2021; 179 JS Gootenberg (567_CR72) 2017; 356 EV Koonin (567_CR32) 2009; 4 I Fonfara (567_CR56) 2016; 532 M Lotfi (567_CR83) 2020; 131 SJJ Brouns (567_CR23) 2008; 321 E Semenova (567_CR30) 2011; 108 JS Chen (567_CR57) 2018; 360 T Yamano (567_CR39) 2016; 165 Y Xu (567_CR11) 2020; 18 P Horvath (567_CR36) 2008; 190 EV Koonin (567_CR20) 2017; 37 E Kim (567_CR37) 2017; 8 T Tsukamoto (567_CR65) 2018; 41 Y Ishino (567_CR14) 1987; 169 OO Abudayyeh (567_CR26) 2017; 550 Y Zhang (567_CR49) 2018; 19 S Nayak (567_CR85) 2017; 89 R Jansen (567_CR18) 2002; 43 FJM Mojica (567_CR33) 2005; 60 EM Kass (567_CR4) 2010; 584 BL Stoddard (567_CR5) 2005; 38 VE Hillary (567_CR53) 2020 DH Haft (567_CR19) 2005; 1 AV Wright (567_CR45) 2016; 23 A Nakata (567_CR15) 1989; 171 Y Jiang (567_CR63) 2021; 6 A Bolotin (567_CR31) 2005; 151 L Cong (567_CR34) 2013; 339 R Liu (567_CR48) 2015; 1 KM Esvelt (567_CR35) 2013; 10 VE Hillary (567_CR50) 2019; 13 DR Rodríguez-Rodríguez (567_CR55) 2019; 43 F Teng (567_CR66) 2019; 20 B Zetsche (567_CR25) 2015; 163 SA Ceasar (567_CR51) 2022; 59
References_xml	– volume: 10 start-page: 1116 issue: 11 year: 2013 ident: 567_CR35 publication-title: Nature Methods doi: 10.1038/nmeth.2681 – volume: 44 start-page: 2200032 year: 2022 ident: 567_CR67 publication-title: BioEssays doi: 10.1002/bies.202200032 – volume: 43 start-page: 1565 issue: 6 year: 2002 ident: 567_CR18 publication-title: Molecular Microbiology doi: 10.1046/j.1365-2958.2002.02839.x – volume: 1 start-page: e60 issue: 6 year: 2005 ident: 567_CR19 publication-title: PLoS Computational Biology doi: 10.1371/journal.pcbi.0010060 – volume: 15 start-page: 1167 year: 2020 ident: 567_CR78 publication-title: ACS Nano. doi: 10.1021/acsnano.0c08165 – volume: 1 start-page: 1 issue: 1 year: 2015 ident: 567_CR48 publication-title: Cell Discovery doi: 10.1038/celldisc.2015.7 – volume: 9 start-page: 2812 issue: 10 year: 2017 ident: 567_CR21 publication-title: Genome Biology and Evolution doi: 10.1093/gbe/evx192 – volume: 169 start-page: 5429 issue: 12 year: 1987 ident: 567_CR14 publication-title: Journal of Bacteriology doi: 10.1128/jb.169.12.5429-5433.1987 – volume: 3 start-page: 1 issue: 1 year: 2012 ident: 567_CR42 publication-title: Nature Communications doi: 10.1038/ncomms1937 – start-page: 219 volume-title: Genome engineering via CRISPR-Cas9 system year: 2020 ident: 567_CR53 doi: 10.1016/B978-0-12-818140-9.00018-0 – volume: 10 start-page: 1245 issue: 6 year: 2021 ident: 567_CR82 publication-title: ACS Synthetic Biology doi: 10.1021/acssynbio.1c00107 – volume: 59 start-page: 2695 issue: 8 year: 1991 ident: 567_CR16 publication-title: Infection and Immunity doi: 10.1128/iai.59.8.2695-2705.1991 – volume: 15 start-page: 1311 issue: 3 year: 2020 ident: 567_CR76 publication-title: Nature Protocols doi: 10.1038/s41596-020-0302-z – volume: 18 start-page: 2401 year: 2020 ident: 567_CR11 publication-title: Computational and Structural Biotechnology Journal doi: 10.1016/j.csbj.2020.08.031 – volume: 1863 start-page: 2333 issue: 9 year: 2016 ident: 567_CR9 publication-title: Biochimica et Biophysica Acta - Molecular Cell Research doi: 10.1016/j.bbamcr.2016.06.009 – volume: 11 start-page: 12 year: 2020 ident: 567_CR81 publication-title: bioRxiv. – volume: 360 start-page: 436 issue: 6387 year: 2018 ident: 567_CR57 publication-title: Science doi: 10.1126/science.aar6245 – volume: 18 start-page: 67 issue: 2 year: 2020 ident: 567_CR58 publication-title: Nature Reviews Microbiology doi: 10.1038/s41579-019-0299-x – volume: 6 start-page: 507 issue: 6 year: 2005 ident: 567_CR1 publication-title: Nature Reviews Genetics doi: 10.1038/nrg1619 – volume: 24 start-page: 8 issue: 1 year: 2020 ident: 567_CR52 publication-title: Animal Cells and Systems doi: 10.1080/19768354.2020.1726462 – volume: 40 start-page: 5569 issue: 12 year: 2012 ident: 567_CR44 publication-title: Nucleic Acids Research doi: 10.1093/nar/gks216 – volume: 360 start-page: 439 issue: 6387 year: 2018 ident: 567_CR73 publication-title: Science doi: 10.1126/science.aaq0179 – volume: 36 start-page: 244 issue: 1 year: 2000 ident: 567_CR17 publication-title: Molecular Microbiology doi: 10.1046/j.1365-2958.2000.01838.x – volume: 167 start-page: 1814 issue: 7 year: 2016 ident: 567_CR38 publication-title: Cell doi: 10.1016/j.cell.2016.11.053 – volume: 4 start-page: 1 issue: 1 year: 2009 ident: 567_CR32 publication-title: Biology Direct doi: 10.1186/1745-6150-4-42 – volume: 578 start-page: 425 issue: 7795 year: 2020 ident: 567_CR69 publication-title: Nature doi: 10.1038/s41586-020-2007-4 – volume: 59 start-page: 107963 year: 2022 ident: 567_CR51 publication-title: Biotechnology Advances doi: 10.1016/j.biotechadv.2022.107963 – volume: 163 start-page: 759 issue: 3 year: 2015 ident: 567_CR25 publication-title: Cell doi: 10.1016/j.cell.2015.09.038 – volume: 471 start-page: 602 issue: 7340 year: 2011 ident: 567_CR27 publication-title: Nature doi: 10.1038/nature09886 – volume: 168 start-page: 121 issue: 1–2 year: 2017 ident: 567_CR71 publication-title: Cell doi: 10.1016/j.cell.2016.12.031 – volume: 17 start-page: 904 issue: 6 year: 2009 ident: 567_CR41 publication-title: Structure doi: 10.1016/j.str.2009.03.019 – volume: 337 start-page: 816 issue: 6096 year: 2012 ident: 567_CR8 publication-title: Science doi: 10.1126/science.1225829 – volume: 538 start-page: 270 issue: 7624 year: 2016 ident: 567_CR70 publication-title: Nature doi: 10.1038/nature19802 – volume: 9 start-page: 467 issue: 6 year: 2011 ident: 567_CR12 publication-title: Nature Reviews Microbiology doi: 10.1038/nrmicro2577 – volume: 362 start-page: 839 issue: 6416 year: 2018 ident: 567_CR40 publication-title: Science doi: 10.1126/science.aav4294 – volume: 315 start-page: 1709 issue: 5819 year: 2007 ident: 567_CR28 publication-title: Science doi: 10.1126/science.1138140 – volume: 584 start-page: 3703 issue: 17 year: 2010 ident: 567_CR4 publication-title: FEBS Letters doi: 10.1016/j.febslet.2010.07.057 – volume: 322 start-page: 1843 issue: 5909 year: 2008 ident: 567_CR24 publication-title: Science doi: 10.1126/science.1165771 – volume: 41 start-page: 1089 issue: 7 year: 2018 ident: 567_CR65 publication-title: Biological and Pharmaceutical Bulletin doi: 10.1248/bpb.b18-00222 – volume: 172 start-page: 1239 issue: 6 year: 2018 ident: 567_CR10 publication-title: Cell doi: 10.1016/j.cell.2017.11.032 – volume: 108 start-page: 10098 issue: 25 year: 2011 ident: 567_CR30 publication-title: Proceedings of the National Academy of Sciences doi: 10.1073/pnas.1104144108 – volume: 30 start-page: 460 issue: 5 year: 2012 ident: 567_CR7 publication-title: Nature Biotechnology doi: 10.1038/nbt.2170 – volume: 60 start-page: 174 issue: 2 year: 2005 ident: 567_CR33 publication-title: Journal of Molecular Evolution doi: 10.1007/s00239-004-0046-3 – volume: 165 start-page: 949 issue: 4 year: 2016 ident: 567_CR39 publication-title: Cell doi: 10.1016/j.cell.2016.04.003 – volume: 20 start-page: 1 issue: 1 year: 2019 ident: 567_CR66 publication-title: Genome Biology doi: 10.1186/s13059-019-1620-8 – volume: 89 start-page: 102 issue: 1 year: 2017 ident: 567_CR85 publication-title: Analytical Chemistry doi: 10.1021/acs.analchem.6b04630 – volume: 65 start-page: 1604 issue: 19 year: 2020 ident: 567_CR62 publication-title: Science Bulletin doi: 10.1016/j.scib.2020.06.014 – volume: 65 start-page: 618 issue: 4 year: 2017 ident: 567_CR75 publication-title: Molecular Cell doi: 10.1016/j.molcel.2016.12.023 – volume: 542 start-page: 237 issue: 7640 year: 2017 ident: 567_CR84 publication-title: Nature doi: 10.1038/nature21059 – volume: 151 start-page: 2551 issue: 8 year: 2005 ident: 567_CR31 publication-title: Microbiology doi: 10.1099/mic.0.28048-0 – volume: 360 start-page: 444 issue: 6387 year: 2018 ident: 567_CR79 publication-title: Science doi: 10.1126/science.aas8836 – volume: 79 start-page: 484 issue: 2 year: 2011 ident: 567_CR43 publication-title: Molecular Microbiology doi: 10.1111/j.1365-2958.2010.07465.x – volume: 532 start-page: 517 issue: 7600 year: 2016 ident: 567_CR56 publication-title: Nature doi: 10.1038/nature17945 – volume: 8 issue: 1 year: 2017 ident: 567_CR37 publication-title: Nature Communications doi: 10.1038/ncomms14500 – volume: 14 start-page: 2986 issue: 10 year: 2019 ident: 567_CR77 publication-title: Nature Protocols doi: 10.1038/s41596-019-0210-2 – volume: 13 start-page: 173 issue: 1 year: 2019 ident: 567_CR50 publication-title: The Open Biotechnology Journal doi: 10.2174/1874070701913010173 – volume: 37 start-page: 67 year: 2017 ident: 567_CR20 publication-title: Current Opinion in Microbiology doi: 10.1016/j.mib.2017.05.008 – volume: 513 start-page: 569 issue: 7519 year: 2014 ident: 567_CR47 publication-title: Nature doi: 10.1038/nature13579 – volume: 19 start-page: 1 issue: 1 year: 2018 ident: 567_CR49 publication-title: Genome Biology doi: 10.1186/s13059-018-1586-y – ident: 567_CR61 – volume: 37 start-page: 708 issue: 5 year: 2019 ident: 567_CR13 publication-title: Biotechnology Advances doi: 10.1016/j.biotechadv.2019.03.016 – volume: 6 start-page: 1086 issue: 3 year: 2021 ident: 567_CR63 publication-title: ACS Sensors doi: 10.1021/acssensors.0c02365 – volume: 23 start-page: 876 issue: 10 year: 2016 ident: 567_CR45 publication-title: Nature Structural & Molecular Biology doi: 10.1038/nsmb.3289 – volume: 179 start-page: 197 year: 2021 ident: 567_CR54 publication-title: Progress in Molecular Biology and Translational Science doi: 10.1016/bs.pmbts.2020.12.017 – volume: 3 start-page: 737 issue: 10 year: 2002 ident: 567_CR2 publication-title: Nature Reviews Genetics doi: 10.1038/nrg908 – volume: 36 start-page: 295 issue: 1 year: 2018 ident: 567_CR86 publication-title: Biotechnology Advances doi: 10.1016/j.biotechadv.2017.11.008 – volume: 171 start-page: 3553 issue: 6 year: 1989 ident: 567_CR15 publication-title: Journal of Bacteriology doi: 10.1128/jb.171.6.3553-3556.1989 – volume: 327 start-page: 167 issue: 5962 year: 2010 ident: 567_CR29 publication-title: Science doi: 10.1126/science.1179555 – volume: 190 start-page: 1401 issue: 4 year: 2008 ident: 567_CR36 publication-title: Journal of Bacteriology doi: 10.1128/JB.01415-07 – volume: 356 start-page: 438 issue: 6336 year: 2017 ident: 567_CR72 publication-title: Science doi: 10.1126/science.aam9321 – volume: 21 start-page: 1179 issue: 11 year: 2021 ident: 567_CR68 publication-title: Expert Review of Molecular Diagnostics doi: 10.1080/14737159.2021.1970535 – volume: 122 start-page: 519 issue: 3 year: 1989 ident: 567_CR3 publication-title: Genetics doi: 10.1093/genetics/122.3.519 – volume: 156 start-page: 935 issue: 5 year: 2014 ident: 567_CR46 publication-title: Cell doi: 10.1016/j.cell.2014.02.001 – volume: 11 start-page: 1589 year: 2020 ident: 567_CR64 publication-title: Frontiers in Plant Science doi: 10.3389/fpls.2020.584151 – volume: 154 start-page: 112068 year: 2020 ident: 567_CR59 publication-title: Biosensors and Bioelectronics doi: 10.1016/j.bios.2020.112068 – year: 2020 ident: 567_CR80 publication-title: BioRxiv doi: 10.1101/2020.02.26.967026 – volume: 38 start-page: 49 issue: 1 year: 2005 ident: 567_CR5 publication-title: Quarterly Reviews of Biophysics doi: 10.1017/S0033583505004063 – volume: 8 start-page: 67 issue: 1 year: 2011 ident: 567_CR6 publication-title: Nature Methods doi: 10.1038/nmeth.1542 – volume: 339 start-page: 819 issue: 6121 year: 2013 ident: 567_CR34 publication-title: Science doi: 10.1126/science.1231143 – volume: 321 start-page: 960 issue: 5891 year: 2008 ident: 567_CR23 publication-title: Science doi: 10.1126/science.1159689 – volume: 38 start-page: 870 issue: 7 year: 2020 ident: 567_CR60 publication-title: Nature Biotechnology doi: 10.1038/s41587-020-0513-4 – volume: 173 start-page: 665 issue: 3 year: 2018 ident: 567_CR74 publication-title: Cell doi: 10.1016/j.cell.2018.02.033 – volume: 550 start-page: 280 issue: 7675 year: 2017 ident: 567_CR26 publication-title: Nature doi: 10.1038/nature24049 – volume: 43 start-page: 1559 issue: 4 year: 2019 ident: 567_CR55 publication-title: International Journal of Molecular Medicine – volume: 23 start-page: 578 issue: 10 year: 2008 ident: 567_CR22 publication-title: Trends in Ecology & Evolution doi: 10.1016/j.tree.2008.06.005 – volume: 131 start-page: 110738 year: 2020 ident: 567_CR83 publication-title: Biomedicine & Pharmacotherapy doi: 10.1016/j.biopha.2020.110738
SSID	ssj0021543
Score	2.6628203
SecondaryResourceType	review_article
Snippet	The clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated protein (CRISPR/Cas) system has altered life science research...
SourceID	pubmedcentral proquest pubmed crossref springer
SourceType	Open Access Repository Aggregation Database Index Database Enrichment Source Publisher
StartPage	311
SubjectTerms	Arrays Biochemistry Biological Techniques Biotechnology Cell Biology Chemistry Chemistry and Materials Science Coronaviruses COVID-19 CRISPR CRISPR-associated proteins CRISPR-Cas Systems Deoxyribonucleic acid DNA Gene Editing - methods Gene sequencing genome Genome editing Genomes gRNA Human Genetics Humans Nucleic acids Nucleotide sequence Protein Science Proteins researchers Review Paper Ribonucleic acid RNA SARS-CoV-2 - genetics Severe acute respiratory syndrome coronavirus 2 Spacers Viral diseases
SummonAdditionalLinks	– databaseName: Biological Science Database dbid: M7P link: http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV1Lb9QwEB5BAQkOPMproSAjcQOrjp3EyQmtVhQ4UK0KlXqLsn6okdqkNCkS_HpmnGx2l4q9cHEOnji2Zmx_zoy_AXgbG2W8zQQ3WWR4rJznpfaO69KKhTfxQngTkk3ow8Ps5CSfDz_c2iGscrkmhoXaNob-ke9LrclhjPvxh4sfnLJGkXd1SKFxE24RS4IKoXvz8cCF8KAPsNeKp4gthksz_dU5SVnMKJad2DA1F5sb0zW0eT1o8i_PadiQDh7871Aewv0BirJpbzuP4Iard-FOn5zy1y7cW6MqfAzdlPVuBNbUDEEj--roznDVnrOytmy65gdnjWezoy_f5kf7s7LNqYhkKFUoYzYncogKJY-76qz67SxD6Mw-ubo5d2zts0_g-ODj99lnPmRs4CbRquM-WujECqPwYJQLm5hSyUThMpGXhBtx6zNxJg0-jCYe_8RJgUYRoWTkEwRHT2Gnbmr3HJj0PvaxkS4RPjRgncqMknZRKm1jMYFoqa7CDHTmlFXjrFgRMZOKC1RxEVRc4DvvxncuejKPrdJ7S_UVw8Rui5XuJvBmrMYpSX6WsnbNFclEWaqI-nCLDC7uKbE5YjvPesMau6TSiFjc0gnoDZMbBYgSfLOmrk4DNXhO-C3Frr9fGueq6_8e6YvtI30JdyVCuz7ybg92ussr9wpum59d1V6-DlPuDw81Lzw priority: 102 providerName: ProQuest
Title	A Review on the Mechanism and Applications of CRISPR/Cas9/Cas12/Cas13/Cas14 Proteins Utilized for Genome Engineering
URI	https://link.springer.com/article/10.1007/s12033-022-00567-0 https://www.ncbi.nlm.nih.gov/pubmed/36163606 https://www.proquest.com/docview/2777173125 https://www.proquest.com/docview/2718633817 https://www.proquest.com/docview/2887619655 https://pubmed.ncbi.nlm.nih.gov/PMC9512960
Volume	65
WOSCitedRecordID	wos000860374300001&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D
hasFullText	1
inHoldings	1
isFullTextHit
isPrint
journalDatabaseRights	– providerCode: PRVAVX databaseName: SpringerLINK Contemporary 1997-Present customDbUrl: eissn: 1559-0305 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0021543 issn: 1073-6085 databaseCode: RSV dateStart: 19970101 isFulltext: true titleUrlDefault: https://link.springer.com/search?facet-content-type=%22Journal%22 providerName: Springer Nature
link	http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3db9MwED_BBgIe-ChfhVEZiTeIlthJnDyWagMeVkUtQ32LUscWkbYELRkS_PXcOR9tGUyCl4siXxzHOds_686_A3jjK6FMHrmOijzl-EIbJ5NGOzLL3bVR_to1yiabkPN5tFrFSXcorO6j3XuXpJ2pN4fdOOUdo-hz4q-UDm7U93G5i2g4LpZfhm0WgoI2rF4KJ0RE0R2V-XMdu8vRFYx5NVTyN3-pXYaOH_zfBzyE-x3sZNPWTh7BDV2O4HabiPLHCO7M-rxvI7i3RVH4GJopa90HrCoZgkV2oumscFGfs6zM2XTL_80qw2aLT8tkcTjL6piEx60UVvosIVKIAjVPm-Ks-KlzhpCZfdBlda7Z1mufwOnx0efZR6fL1OCoQIrGMd5aBrmrBG6IYjcPVCZ4IHB6iDPCi7jkKT_iCi9KEn9_oLmLxuChpmcCBEVPYa-sSv0cGDfGN77iOnCNrSDXIlKC5-tMyNx3x-D1PyxVHY05ZdM4SzcEzNTPKfZzavs5xWfeDs98a0k8rtU-6O0g7QZ0nXIpKV4B4eAYXg_F-GfIv5KVurokHS8KBVEeXqODk3pILI5Yz7PWtIYmidAj9rZwDHLH6AYFogLfLSmLr5YSPCbcFmLT3_Wmt2n637_0xb-pv4S7HCFeG4F3AHvNxaV-BbfU96aoLyZwU66kldEE9t8fzZMF3p3whKRs5XJih-ovS9MvKg
linkProvider	Springer Nature
linkToHtml	http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMw1V1Lb9NAEF6VAgIOPMorUGCR4FRWtXdtr31AKAqURm2jqLRSb8bZh7DU2qV2QeVH8RuZWcdOQkVuPXDZHDzerJ1vZ7_NzH5DyJtACWV17DEV-4oFwliWSWuYzLQ3sSqYeFa5YhNyNIqPjpLxCvndnoXBtMrWJzpHrUuF_5FvcikxYAzr8YfT7wyrRmF0tS2h0cBix1z8hC1b9X74EX7ft5xvfToYbLNpVQGmQilqZv2JDLWnBJD3xNOhygQPBUA5yZDbgHtWQcwVfCiJWvOh4R4M3AdL34awgEO_18h1oBE8dqmC426DB3SkSeiXgkXAZaaHdJqjehyrpmHuPKpvSuYtLoSX2O3lJM2_IrVuAdy697-9uvvk7pRq034zNx6QFVOskZtN8c2LNXJnTorxIan7tAmT0LKgQIrpnsEz0Xl1QrNC0_5cnJ-Wlg72h1_G-5uDrEqw8blrhWsDOkbxixwsD-v8OP9lNIWtAf1sivLE0LmvfUQOr-QFPCarRVmYp4RyawMbKG5Cz7oOtBGxElxPMiF14PWI38IjVVO5dqwacpzOhKYRUilAKnWQSuGeje6e00asZKn1eguXdOq4qnSGlR553V0Gl4NxpKww5Tna-HEkUNpxiQ0sXhGqVUI_Txogd0MSkY8qdVGPyAWIdwYoeb54pci_OenzBPlpBEN_106G2dD__aTPlj_pK3Jr-2BvN90djnaek9scaGyTZbhOVuuzc_OC3FA_6rw6e-mmOyVfr3qS_AG0H4iI
linkToPdf	http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMw1V1Lb9QwELZKeQgOPMproYCR4ATWJnYSJweEVlsWVoVVVKjUW8g6tojUJqVJQeWn8euYcR67S8XeeuDiHDJx7Ow34_HO-BtCXnhKKJOFDlOhq5gntGGpNJrJNHPmRnlzxyhbbELOZuHBQRRvkN_dWRhMq-xsojXUWanwP_IhlxIDxrAeD02bFhHvTN4ef2dYQQojrV05jQYiu_rsJ2zfqjfTHfitX3I-efdl_IG1FQaY8qWomXHn0s8cJcCRj5zMV6ngvgBYRyn6OWCqlRdyBRclkXfe19yBSbgg6RofFnPo9xK5LJG03KYNxv1mD1yTJrlfChaAX9Me2GmO7XGsoIZ59MjEKZmzuiie83TPJ2z-FbW1i-Hk1v_8GW-Tm60LTkeNztwhG7rYIlebopxnW-TGEkXjXVKPaBM-oWVBwVmmnzSelc6rI5oWGR0txf9paeh4b_o53huO0yrCxuW2Fbb1aIykGDlI7tf5Yf5LZxS2DPS9LsojTZdee4_sX8gHuE82i7LQDwnlxnjGU1z7jrEdZFqESvBsngqZec6AuB1UEtXSuGM1kcNkQUCN8EoAXomFVwLPvOqfOW5ITNZKb3fQSVqDViUL3AzI8_42mCKML6WFLk9Rxg0DgZSPa2RgUQuQxRL6edCAuh-SCFxkrwsGRK7AvRdAKvTVO0X-zVKiR-i3BjD0151iLIb-75k-Wj_TZ-Qa6EbycTrbfUyuc_Bum-TDbbJZn5zqJ-SK-lHn1clTq_mUfL1oHfkDHD2RRQ
openUrl	ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=A+Review+on+the+Mechanism+and+Applications+of+CRISPR%2FCas9%2FCas12%2FCas13%2FCas14+Proteins+Utilized+for+Genome+Engineering&rft.jtitle=Molecular+biotechnology&rft.au=Hillary%2C+V.+Edwin&rft.au=Ceasar%2C+S.+Antony&rft.date=2023-03-01&rft.pub=Springer+US&rft.issn=1073-6085&rft.eissn=1559-0305&rft.volume=65&rft.issue=3&rft.spage=311&rft.epage=325&rft_id=info:doi/10.1007%2Fs12033-022-00567-0&rft.externalDocID=10_1007_s12033_022_00567_0
thumbnail_l	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1073-6085&client=summon
thumbnail_m	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1073-6085&client=summon
thumbnail_s	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1073-6085&client=summon