Contamination in Low Microbial Biomass Microbiome Studies: Issues and Recommendations

Next-generation sequencing approaches in microbiome research have allowed surveys of microbial communities, their genomes, and their functions with higher sensitivity than ever before. However, this sensitivity is a double-edged sword because these tools also efficiently detect contaminant DNA and c...

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Vydáno v:	Trends in microbiology (Regular ed.) Ročník 27; číslo 2; s. 105 - 117
Hlavní autoři:	Eisenhofer, Raphael, Minich, Jeremiah J., Marotz, Clarisse, Cooper, Alan, Knight, Rob, Weyrich, Laura S.
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	England Elsevier Ltd 01.02.2019 Elsevier Science Ltd
Témata:	Biomass Contaminants Contamination cross contamination Deoxyribonucleic acid DNA DNA Contamination DNA, Bacterial - analysis DNA, Bacterial - genetics genome Genomes High-Throughput Nucleotide Sequencing Humans laboratory contamination low biomass Metagenomics methodology Microbial activity microbial biomass microbial communities Microbial contamination Microbiological Techniques - methods Microbiological Techniques - standards microbiome Microbiomes microbiota Microbiota - genetics Microorganisms Next-generation sequencing Reproducibility of Results Sensitivity Specimen Handling surveys microbiota laboratory contamination DNA contamination microbiome methodology low biomass
ISSN:	0966-842X, 1878-4380, 1878-4380
On-line přístup:	Získat plný text
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