A Python script to merge Sanger sequences

Merging Sanger sequences is frequently needed during the gene cloning process. In this study, we provide a Python script that is able to assemble multiple overlapping Sanger sequences. The script utilizes the overlapping regions within the tandem Sanger sequences to merge the Sanger sequences. The r...

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Bibliographic Details
Published in:PeerJ (San Francisco, CA) Vol. 9; p. e11354
Main Authors: Chen, Cen, Lu, Bingguo, Huang, Xiaofang, Bi, Chuyun, Zhao, Lili, Hu, Yunzhuo, Chen, Xuanyang, Lin, Shiqiang, Huang, Kai
Format: Journal Article
Language:English
Published: United States PeerJ. Ltd 27.04.2021
PeerJ, Inc
PeerJ Inc
Subjects:
Algorithms
Bioinformatics
Cloning
Computational Science
DNA sequencing
Genes
Genetics
Genomes
Genomics
Merge
Molecular Biology
Nucleotide sequence
Nucleotide sequencing
Python script
Sanger sequencing
Software
United States > US
Merge
Python script
Sanger sequencing
ISSN:2167-8359, 2167-8359
Online Access:Get full text
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Summary:Merging Sanger sequences is frequently needed during the gene cloning process. In this study, we provide a Python script that is able to assemble multiple overlapping Sanger sequences. The script utilizes the overlapping regions within the tandem Sanger sequences to merge the Sanger sequences. The results demonstrate that the script can produce the merged sequence from the input Sanger sequences in a single run. The script offers a simple and free method for merging Sanger sequences and is useful for gene cloning.
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ISSN:2167-8359
2167-8359
DOI:10.7717/peerj.11354