Base editors for simultaneous introduction of C-to-T and A-to-G mutations

We describe base editors that combine both cytosine and adenine base-editing functions. A codon-optimized fusion of the cytosine deaminase PmCDA1, the adenosine deaminase TadA and a Cas9 nickase (Target-ACEmax) showed a high median simultaneous C-to-T and A-to-G editing activity at 47 genomic target...

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Bibliographic Details
Published in:	Nature biotechnology Vol. 38; no. 7; pp. 865 - 869
Main Authors:	Sakata, Rina C, Ishiguro, Soh, Mori, Hideto, Tanaka, Mamoru, Tatsuno, Kenji, Ueda, Hiroki, Yamamoto, Shogo, Seki, Motoaki, Masuyama, Nanami, Nishida, Keiji, Nishimasu, Hiroshi, Arakawa, Kazuharu, Kondo, Akihiko, Nureki, Osamu, Tomita, Masaru, Aburatani, Hiroyuki, Yachie, Nozomu
Format:	Journal Article
Language:	English
Published:	United States Nature Publishing Group 01.07.2020
Subjects:	Adenine Adenine - metabolism Adenosine Adenosine deaminase Adenosine Deaminase - genetics CRISPR-Associated Protein 9 - genetics CRISPR-Cas Systems - genetics Cytosine Cytosine - metabolism Cytosine deaminase Deoxyribonuclease I - genetics Deoxyribonucleic acid DNA DNA - genetics Editing Gene Editing Genome, Human - genetics Glycoproteins - genetics Guanine - metabolism HEK293 Cells Humans Mutation Mutation - genetics Nuclear Proteins - genetics Ribonucleic acid RNA RNA - genetics
ISSN:	1087-0156, 1546-1696, 1546-1696
Online Access:	Get full text
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