MicroRNA profiling of the bovine alveolar macrophage response to Mycobacterium bovis infection suggests pathogen survival is enhanced by microRNA regulation of endocytosis and lysosome trafficking

Mycobacterium bovis, the causative agent of bovine tuberculosis, a major problem for global agriculture, spreads via an airborne route and is taken up by alveolar macrophages (AM) in the lung. Here, we describe the first next-generation sequencing (RNA-seq) approach to temporally profile miRNA expre...

Celý popis

Uloženo v:

Podrobná bibliografie
Vydáno v:	Tuberculosis (Edinburgh, Scotland) Ročník 95; číslo 1; s. 60 - 67
Hlavní autoři:	Vegh, Peter, Magee, David A., Nalpas, Nicolas C., Bryan, Kenneth, McCabe, Matthew S., Browne, John A., Conlon, Kevin M., Gordon, Stephen V., Bradley, Daniel G., MacHugh, David E., Lynn, David J.
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	Scotland Elsevier Ltd 01.01.2015
Témata:	Alveolar macrophage Animals Bos taurus Cattle Cells, Cultured Down-Regulation Endocytosis - immunology Gene Expression - genetics Gene Expression - immunology Gene Expression Profiling - methods Immunity, Innate - immunology Infectious Disease Interleukin-1 Receptor-Associated Kinases - antagonists & inhibitors Lysosomes - immunology Macrophages, Alveolar - immunology Male microRNA MicroRNAs - genetics MicroRNAs - immunology MicroRNAs - physiology Mycobacterium bovis Mycobacterium bovis - immunology Pulmonary/Respiratory Real-Time Polymerase Chain Reaction - methods RNA, Bacterial - genetics RNA, Bacterial - immunology RNA-seq Sequence Analysis, RNA - methods Transfection - methods Transforming Growth Factor beta2 - antagonists & inhibitors Tuberculosis Tuberculosis, Bovine - immunology Tuberculosis, Pulmonary - immunology Up-Regulation microRNA Tuberculosis Bos taurus RNA-seq Mycobacterium bovis Alveolar macrophage
ISSN:	1472-9792, 1873-281X, 1873-281X
On-line přístup:	Získat plný text
Tagy:	Přidat tag Žádné tagy, Buďte první, kdo vytvoří štítek k tomuto záznamu!

Popis
Shrnutí:	Mycobacterium bovis, the causative agent of bovine tuberculosis, a major problem for global agriculture, spreads via an airborne route and is taken up by alveolar macrophages (AM) in the lung. Here, we describe the first next-generation sequencing (RNA-seq) approach to temporally profile miRNA expression in primary bovine AMs post-infection with M. bovis. One, six, and forty miRNAs were identified as significantly differentially expressed at 2, 24 and 48 h post-infection, respectively. The differential expression of three miRNAs (bta-miR-142-5p, bta-miR-146a, and bta-miR-423-3p) was confirmed by RT-qPCR. Pathway analysis of the predicted mRNA targets of differentially expressed miRNAs suggests that these miRNAs preferentially target several pathways that are functionally relevant for mycobacterial pathogenesis, including endocytosis and lysosome trafficking, IL-1 signalling and the TGF-β pathway. Over-expression studies using a bovine macrophage cell-line (Bomac) reveal the targeting of two key genes in the innate immune response to M. bovis, IL-1 receptor-associated kinase 1 (IRAK1) and TGF-β receptor 2 (TGFBR2), by miR-146. Taken together, our study suggests that miRNAs play a key role in tuning the complex interplay between M. bovis survival strategies and the host immune response.
Bibliografie:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1472-9792 1873-281X 1873-281X
DOI:	10.1016/j.tube.2014.10.011